Restriction map for
pFN21AE9434
(Linear) (Six-base) MAPSORT of: KIEE9434.seq Check: 8247 from: 1 to: 1011
KIEE9434.orf
With 182 enzymes: SgfI *
December 27, 2011 16:40 ..
AflIII A'CryG_T
Cuts at: 0 386 1011
Size: 386 625
AleI CACnn'nnGTG
Cuts at: 0 665 1011
Size: 665 346
ApaLI G'TGCA_C
Cuts at: 0 162 465 1011
Size: 162 303 546
Fragments arranged by size:
546 303 162
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 883 916 1011
Size: 883 33 95
Fragments arranged by size:
883 95 33
BanI G'GyrC_C
Cuts at: 0 117 520 804 1011
Size: 117 403 284 207
Fragments arranged by size:
403 284 207 117
BanII G_rGCy'C
Cuts at: 0 794 1011
Size: 794 217
BbeI G_GCGC'C
Cuts at: 0 121 524 1011
Size: 121 403 487
Fragments arranged by size:
487 403 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 318 504 1011
Size: 318 186 507
Fragments arranged by size:
507 318 186
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 597 631 1011
Size: 597 34 380
Fragments arranged by size:
597 380 34
BclI T'GATC_A
Cuts at: 0 623 773 1011
Size: 623 150 238
Fragments arranged by size:
623 238 150
BglI GCCn_nnn'nGGC
Cuts at: 0 971 1011
Size: 971 40
BlpI GC'TnA_GC
Cuts at: 0 283 298 334 1011
Size: 283 15 36 677
Fragments arranged by size:
677 283 36 15
Bme1580I G_kGCm'C
Cuts at: 0 43 166 469 809 1011
Size: 43 123 303 340 202
Fragments arranged by size:
340 303 202 123 43
BmgBI CAC'GTC
Cuts at: 0 621 1011
Size: 621 390
BmrI ACTGGGnnnn_n'
Cuts at: 0 659 831 1011
Size: 659 172 180
Fragments arranged by size:
659 180 172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 813 1011
Size: 813 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1011
Size: 706 305
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 571 1011
Size: 562 9 440
Fragments arranged by size:
562 440 9
BsaAI yAC'GTr
Cuts at: 0 146 389 572 1011
Size: 146 243 183 439
Fragments arranged by size:
439 243 183 146
BsaHI Gr'CG_yC
Cuts at: 0 50 118 197 521 1011
Size: 50 68 79 324 490
Fragments arranged by size:
490 324 79 68 50
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 931 961 1011
Size: 931 30 50
Fragments arranged by size:
931 50 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 948 1011
Size: 703 245 63
Fragments arranged by size:
703 245 63
BseYI C'CCAG_C
Cuts at: 0 311 411 1011
Size: 311 100 600
Fragments arranged by size:
600 311 100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 379 495 552 643 922 1011
Size: 76 303 116 57 91 279 89
Fragments arranged by size:
303 279 116 91 89 76 57
BsiEI CG_ry'CG
Cuts at: 0 228 309 1011
Size: 228 81 702
Fragments arranged by size:
702 228 81
BsiHKAI G_wGCw'C
Cuts at: 0 166 469 563 587 794 1011
Size: 166 303 94 24 207 217
Fragments arranged by size:
303 217 207 166 94 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 57 1011
Size: 57 954
Bsp1286I G_dGCh'C
Cuts at: 0 43 166 469 563 587 794 809 1011
Size: 43 123 303 94 24 207 15 202
Fragments arranged by size:
303 207 202 123 94 43 24 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 665 1011
Size: 164 501 346
Fragments arranged by size:
501 346 164
BsrBI CCG'CTC
Cuts at: 0 183 1011
Size: 183 828
BsrDI GCAATG_nn'
Cuts at: 0 603 1011
Size: 603 408
BsrFI r'CCGG_y
Cuts at: 0 120 193 216 479 999 1011
Size: 120 73 23 263 520 12
Fragments arranged by size:
520 263 120 73 23 12
BssSI C'ACGA_G
Cuts at: 0 6 462 640 1011
Size: 6 456 178 371
Fragments arranged by size:
456 371 178 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 473 911 1011
Size: 473 438 100
Fragments arranged by size:
473 438 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 72 786 843 961 1011
Size: 72 714 57 118 50
Fragments arranged by size:
714 118 72 57 50
BtgI C'CryG_G
Cuts at: 0 167 320 800 1011
Size: 167 153 480 211
Fragments arranged by size:
480 211 167 153
DraIII CAC_nnn'GTG
Cuts at: 0 473 1011
Size: 473 538
EaeI y'GGCC_r
Cuts at: 0 191 290 317 484 1011
Size: 191 99 27 167 527
Fragments arranged by size:
527 191 167 99 27
EarI CTCTTCn'nnn_
Cuts at: 0 133 799 1011
Size: 133 666 212
Fragments arranged by size:
666 212 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 97 1011
Size: 97 914
EcoICRI GAG'CTC
Cuts at: 0 792 1011
Size: 792 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1011
Size: 706 305
EcoO109I rG'GnC_Cy
Cuts at: 0 743 1011
Size: 743 268
HaeII r_GCGC'y
Cuts at: 0 121 127 373 498 524 1011
Size: 121 6 246 125 26 487
Fragments arranged by size:
487 246 125 121 26 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 935 967 1011
Size: 935 32 44
Fragments arranged by size:
935 44 32
HincII GTy'rAC
Cuts at: 0 174 1011
Size: 174 837
KasI G'GCGC_C
Cuts at: 0 117 520 1011
Size: 117 403 491
Fragments arranged by size:
491 403 117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 890 1011
Size: 89 801 121
Fragments arranged by size:
801 121 89
MslI CAynn'nnrTG
Cuts at: 0 619 665 1011
Size: 619 46 346
Fragments arranged by size:
619 346 46
MspA1I CmG'CkG
Cuts at: 0 210 311 333 453 1011
Size: 210 101 22 120 558
Fragments arranged by size:
558 210 120 101 22
NaeI GCC'GGC
Cuts at: 0 122 195 218 481 1011
Size: 122 73 23 263 530
Fragments arranged by size:
530 263 122 73 23
NarI GG'CG_CC
Cuts at: 0 118 521 1011
Size: 118 403 490
Fragments arranged by size:
490 403 118
NgoMIV G'CCGG_C
Cuts at: 0 120 193 216 479 1011
Size: 120 73 23 263 532
Fragments arranged by size:
532 263 120 73 23
NspI r_CATG'y
Cuts at: 0 180 1011
Size: 180 831
PmlI CAC'GTG
Cuts at: 0 146 389 1011
Size: 146 243 622
Fragments arranged by size:
622 243 146
PpuMI rG'GwC_Cy
Cuts at: 0 743 1011
Size: 743 268
PvuII CAG'CTG
Cuts at: 0 453 1011
Size: 453 558
SacI G_AGCT'C
Cuts at: 0 794 1011
Size: 794 217
SapI GCTCTTCn'nnn_
Cuts at: 0 133 799 1011
Size: 133 666 212
Fragments arranged by size:
666 212 133
SfoI GGC'GCC
Cuts at: 0 119 522 1011
Size: 119 403 489
Fragments arranged by size:
489 403 119
SgrAI Cr'CCGG_yG
Cuts at: 0 120 1011
Size: 120 891
SmlI C'TyrA_G
Cuts at: 0 577 586 1011
Size: 577 9 425
Fragments arranged by size:
577 425 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 303 1011
Size: 303 708
TatI w'GTAC_w
Cuts at: 0 878 1011
Size: 878 133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 931 963 1011
Size: 931 32 48
Fragments arranged by size:
931 48 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 1011
Size: 285 726
Enzymes that do cut:
AflIII AleI ApaLI BaeI BanI BanII BbeI BbsI
BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BtgI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PmlI PpuMI
PvuII SacI SapI SfoI SgrAI SmlI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvaI AvrII BamHI BbvCI BciVI BfrBI
BglII BmtI Bpu10I BsaI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrGI BssHII BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SgfI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI