Restriction map for
pFN21AE9433
(Linear) (Six-base) MAPSORT of: KIEE9433.seq Check: 546 from: 1 to: 1545
KIEE9433.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 580 1231 1545
Size: 580 651 314
Fragments arranged by size:
651 580 314
AccI GT'mk_AC
Cuts at: 0 860 1545
Size: 860 685
AfeI AGC'GCT
Cuts at: 0 731 1545
Size: 731 814
AflIII A'CryG_T
Cuts at: 0 50 1545
Size: 50 1495
AgeI A'CCGG_T
Cuts at: 0 1019 1545
Size: 1019 526
AleI CACnn'nnGTG
Cuts at: 0 136 208 1545
Size: 136 72 1337
Fragments arranged by size:
1337 136 72
AlwNI CAG_nnn'CTG
Cuts at: 0 1107 1315 1545
Size: 1107 208 230
Fragments arranged by size:
1107 230 208
ApaI G_GGCC'C
Cuts at: 0 504 693 1545
Size: 504 189 852
Fragments arranged by size:
852 504 189
ApoI r'AATT_y
Cuts at: 0 361 1545
Size: 361 1184
AvaI C'yCGr_G
Cuts at: 0 91 255 1092 1545
Size: 91 164 837 453
Fragments arranged by size:
837 453 164 91
BanI G'GyrC_C
Cuts at: 0 58 1302 1470 1545
Size: 58 1244 168 75
Fragments arranged by size:
1244 168 75 58
BanII G_rGCy'C
Cuts at: 0 504 693 1545
Size: 504 189 852
Fragments arranged by size:
852 504 189
BbeI G_GCGC'C
Cuts at: 0 62 1545
Size: 62 1483
BbsI GAAGACnn'nnnn_
Cuts at: 0 813 888 1545
Size: 813 75 657
Fragments arranged by size:
813 657 75
BbvCI CC'TCA_GC
Cuts at: 0 301 397 1064 1545
Size: 301 96 667 481
Fragments arranged by size:
667 481 301 96
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 72 106 1545
Size: 72 34 1439
Fragments arranged by size:
1439 72 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1302 1545
Size: 1302 243
BglII A'GATC_T
Cuts at: 0 1136 1545
Size: 1136 409
BlpI GC'TnA_GC
Cuts at: 0 1188 1545
Size: 1188 357
Bme1580I G_kGCm'C
Cuts at: 0 287 504 693 842 1475 1545
Size: 287 217 189 149 633 70
Fragments arranged by size:
633 287 217 189 149 70
BmgBI CAC'GTC
Cuts at: 0 566 1545
Size: 566 979
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 606 1129 1249 1545
Size: 469 137 523 120 296
Fragments arranged by size:
523 469 296 137 120
Bpu10I CC'TnA_GC
Cuts at: 0 301 397 1064 1273 1545
Size: 301 96 667 209 272
Fragments arranged by size:
667 301 272 209 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 1393 1545
Size: 1393 152
BsaBI GATnn'nnATC
Cuts at: 0 493 679 1545
Size: 493 186 866
Fragments arranged by size:
866 493 186
BsaHI Gr'CG_yC
Cuts at: 0 59 1545
Size: 59 1486
BsaWI w'CCGG_w
Cuts at: 0 734 1019 1545
Size: 734 285 526
Fragments arranged by size:
734 526 285
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1373 1545
Size: 1373 172
BseYI C'CCAG_C
Cuts at: 0 773 1081 1545
Size: 773 308 464
Fragments arranged by size:
773 464 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 558 775 1545
Size: 160 398 217 770
Fragments arranged by size:
770 398 217 160
BsiHKAI G_wGCw'C
Cuts at: 0 875 1279 1545
Size: 875 404 266
Fragments arranged by size:
875 404 266
Bsp1286I G_dGCh'C
Cuts at: 0 287 504 693 842 875 1279 1475 1545
Size: 287 217 189 149 33 404 196 70
Fragments arranged by size:
404 287 217 196 189 149 70 33
BspEI T'CCGG_A
Cuts at: 0 734 1545
Size: 734 811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 132 580 1231 1545
Size: 132 448 651 314
Fragments arranged by size:
651 448 314 132
BsrBI CCG'CTC
Cuts at: 0 1494 1545
Size: 1494 51
BsrFI r'CCGG_y
Cuts at: 0 1019 1305 1545
Size: 1019 286 240
Fragments arranged by size:
1019 286 240
BsrGI T'GTAC_A
Cuts at: 0 491 1545
Size: 491 1054
BssHII G'CGCG_C
Cuts at: 0 86 1545
Size: 86 1459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 816 1545
Size: 816 729
BstYI r'GATC_y
Cuts at: 0 20 329 1136 1545
Size: 20 309 807 409
Fragments arranged by size:
807 409 309 20
BtgI C'CryG_G
Cuts at: 0 635 809 1545
Size: 635 174 736
Fragments arranged by size:
736 635 174
BtsI GCAGTG_nn'
Cuts at: 0 515 908 1545
Size: 515 393 637
Fragments arranged by size:
637 515 393
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 624 659 1545
Size: 624 35 886
Fragments arranged by size:
886 624 35
DraIII CAC_nnn'GTG
Cuts at: 0 609 1545
Size: 609 936
DrdI GACnn_nn'nnGTC
Cuts at: 0 892 1545
Size: 892 653
EaeI y'GGCC_r
Cuts at: 0 515 1545
Size: 515 1030
EarI CTCTTCn'nnn_
Cuts at: 0 349 673 1157 1445 1545
Size: 349 324 484 288 100
Fragments arranged by size:
484 349 324 288 100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 606 1129 1249 1545
Size: 469 137 523 120 296
Fragments arranged by size:
523 469 296 137 120
EcoO109I rG'GnC_Cy
Cuts at: 0 690 998 1545
Size: 690 308 547
Fragments arranged by size:
690 547 308
HaeII r_GCGC'y
Cuts at: 0 62 733 1545
Size: 62 671 812
Fragments arranged by size:
812 671 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 1276 1308 1545
Size: 457 32 787 32 237
Fragments arranged by size:
787 457 237 32 32
HincII GTy'rAC
Cuts at: 0 194 1545
Size: 194 1351
KasI G'GCGC_C
Cuts at: 0 58 1545
Size: 58 1487
MluI A'CGCG_T
Cuts at: 0 50 1545
Size: 50 1495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1545
Size: 708 837
MscI TGG'CCA
Cuts at: 0 517 1545
Size: 517 1028
MslI CAynn'nnrTG
Cuts at: 0 136 208 814 1545
Size: 136 72 606 731
Fragments arranged by size:
731 606 136 72
MspA1I CmG'CkG
Cuts at: 0 114 777 1192 1545
Size: 114 663 415 353
Fragments arranged by size:
663 415 353 114
NarI GG'CG_CC
Cuts at: 0 59 1545
Size: 59 1486
NcoI C'CATG_G
Cuts at: 0 809 1545
Size: 809 736
PflMI CCAn_nnn'nTGG
Cuts at: 0 510 1107 1545
Size: 510 597 438
Fragments arranged by size:
597 510 438
PfoI T'CCnGG_A
Cuts at: 0 1106 1545
Size: 1106 439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 1545
Size: 739 32 774
Fragments arranged by size:
774 739 32
PpuMI rG'GwC_Cy
Cuts at: 0 998 1545
Size: 998 547
PspOMI G'GGCC_C
Cuts at: 0 500 689 1545
Size: 500 189 856
Fragments arranged by size:
856 500 189
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 109 141 1070 1102 1545
Size: 109 32 929 32 443
Fragments arranged by size:
929 443 109 32 32
PstI C_TGCA'G
Cuts at: 0 1355 1545
Size: 1355 190
PvuII CAG'CTG
Cuts at: 0 114 1192 1545
Size: 114 1078 353
Fragments arranged by size:
1078 353 114
SanDI GG'GwC_CC
Cuts at: 0 998 1545
Size: 998 547
SapI GCTCTTCn'nnn_
Cuts at: 0 349 1445 1545
Size: 349 1096 100
Fragments arranged by size:
1096 349 100
SexAI A'CCwGG_T
Cuts at: 0 605 1545
Size: 605 940
SfcI C'TryA_G
Cuts at: 0 1351 1545
Size: 1351 194
SfoI GGC'GCC
Cuts at: 0 60 1545
Size: 60 1485
StyI C'CwwG_G
Cuts at: 0 596 809 1205 1433 1475 1545
Size: 596 213 396 228 42 70
Fragments arranged by size:
596 396 228 213 70 42
TatI w'GTAC_w
Cuts at: 0 491 1045 1178 1545
Size: 491 554 133 367
Fragments arranged by size:
554 491 367 133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 1545
Size: 37 32 1476
Fragments arranged by size:
1476 37 32
Enzymes that do cut:
AarI AccI AfeI AflIII AgeI AleI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrGI BssHII BstXI BstYI
BtgI BtsI CspCI DraIII DrdI EaeI EarI Eco57MI
EcoO109I HaeII Hin4I HincII KasI MluI MmeI MscI
MslI MspA1I NarI NcoI PflMI PfoI PpiI PpuMI
PspOMI PsrI PstI PvuII SanDI SapI SexAI SfcI
SfoI StyI TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AhdI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BclI
BfrBI BglI BmrI BmtI BplI BpuEI BsaAI BsaXI
BsiEI BsiWI BsmI BsmBI BspHI BsrDI BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI