Restriction map for
pFN21AE9432
(Linear) (Six-base) MAPSORT of: KIEE9432.seq Check: 2182 from: 1 to: 2442
KIEE9432.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1212 2442
Size: 1212 1230
AatII G_ACGT'C
Cuts at: 0 1229 2442
Size: 1229 1213
Acc65I G'GTAC_C
Cuts at: 0 2017 2442
Size: 2017 425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1322 1630 1725 1979 2442
Size: 955 367 308 95 254 463
Fragments arranged by size:
955 463 367 308 254 95
AfeI AGC'GCT
Cuts at: 0 303 2442
Size: 303 2139
AflIII A'CryG_T
Cuts at: 0 614 1905 2442
Size: 614 1291 537
Fragments arranged by size:
1291 614 537
AleI CACnn'nnGTG
Cuts at: 0 644 2437 2442
Size: 644 1793 5
Fragments arranged by size:
1793 644 5
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 715 2442
Size: 715 1727
AlwNI CAG_nnn'CTG
Cuts at: 0 54 495 2341 2442
Size: 54 441 1846 101
Fragments arranged by size:
1846 441 101 54
ApaI G_GGCC'C
Cuts at: 0 1346 1678 1824 2115 2391 2442
Size: 1346 332 146 291 276 51
Fragments arranged by size:
1346 332 291 276 146 51
ApaLI G'TGCA_C
Cuts at: 0 1170 1332 2442
Size: 1170 162 1110
Fragments arranged by size:
1170 1110 162
AvaI C'yCGr_G
Cuts at: 0 1050 1319 2053 2442
Size: 1050 269 734 389
Fragments arranged by size:
1050 734 389 269
BanI G'GyrC_C
Cuts at: 0 715 2017 2442
Size: 715 1302 425
Fragments arranged by size:
1302 715 425
BanII G_rGCy'C
Cuts at: 0 343 1346 1601 1678 1824 2054 2115 2308
Size: 343 1003 255 77 146 230 61 193
Cuts at: 2308 2391 2442
Size: 83 51
Fragments arranged by size:
1003 343 255 230 193 146 83 77
61 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 1971 2204 2442
Size: 1971 233 238
Fragments arranged by size:
1971 238 233
BclI T'GATC_A
Cuts at: 0 1440 2442
Size: 1440 1002
BglI GCCn_nnn'nGGC
Cuts at: 0 724 737 2442
Size: 724 13 1705
Fragments arranged by size:
1705 724 13
BglII A'GATC_T
Cuts at: 0 1662 2442
Size: 1662 780
BlpI GC'TnA_GC
Cuts at: 0 1814 2442
Size: 1814 628
Bme1580I G_kGCm'C
Cuts at: 0 1174 1336 1346 1678 1824 1893 2115 2192
Size: 1174 162 10 332 146 69 222 77
Cuts at: 2192 2391 2442
Size: 199 51
Fragments arranged by size:
1174 332 222 199 162 146 77 69
51 10
BmgBI CAC'GTC
Cuts at: 0 545 615 2442
Size: 545 70 1827
Fragments arranged by size:
1827 545 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 286 1017 1323 2179 2366 2442
Size: 286 731 306 856 187 76
Fragments arranged by size:
856 731 306 286 187 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 2442
Size: 1083 1359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 899 1371 1603 2044 2442
Size: 559 340 472 232 441 398
Fragments arranged by size:
559 472 441 398 340 232
Bpu10I CC'TnA_GC
Cuts at: 0 1265 2348 2442
Size: 1265 1083 94
Fragments arranged by size:
1265 1083 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 1988 2442
Size: 1387 601 454
Fragments arranged by size:
1387 601 454
BsaI GGTCTCn'nnnn_
Cuts at: 0 57 1499 2442
Size: 57 1442 943
Fragments arranged by size:
1442 943 57
BsaHI Gr'CG_yC
Cuts at: 0 1226 2442
Size: 1226 1216
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1496 1526 2442
Size: 1496 30 916
Fragments arranged by size:
1496 916 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1048 1051 1072 1338 1423 1517 1609 1812
Size: 1048 3 21 266 85 94 92 203
Cuts at: 1812 2442
Size: 630
Fragments arranged by size:
1048 630 266 203 94 92 85 21
3
BseYI C'CCAG_C
Cuts at: 0 495 811 929 1525 1773 1927 2442
Size: 495 316 118 596 248 154 515
Fragments arranged by size:
596 515 495 316 248 154 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 1829 1930 2442
Size: 606 1223 101 512
Fragments arranged by size:
1223 606 512 101
BsiHKAI G_wGCw'C
Cuts at: 0 343 1174 1336 2308 2442
Size: 343 831 162 972 134
Fragments arranged by size:
972 831 343 162 134
BsmI GAATG_Cn'
Cuts at: 0 843 2442
Size: 843 1599
Bsp1286I G_dGCh'C
Cuts at: 0 343 1174 1336 1346 1601 1678 1824 1893
Size: 343 831 162 10 255 77 146 69
Cuts at: 1893 2054 2115 2192 2308 2391 2442
Size: 161 61 77 116 83 51
Fragments arranged by size:
831 343 255 162 161 146 116 83
77 77 69 61 51 10
BspHI T'CATG_A
Cuts at: 0 72 539 2442
Size: 72 467 1903
Fragments arranged by size:
1903 467 72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 494 1212 2442
Size: 494 718 1230
Fragments arranged by size:
1230 718 494
BsrBI CCG'CTC
Cuts at: 0 1608 1826 2393 2442
Size: 1608 218 567 49
Fragments arranged by size:
1608 567 218 49
BsrDI GCAATG_nn'
Cuts at: 0 946 2442
Size: 946 1496
BsrFI r'CCGG_y
Cuts at: 0 2276 2442
Size: 2276 166
BsrGI T'GTAC_A
Cuts at: 0 895 2442
Size: 895 1547
BstEII G'GTnAC_C
Cuts at: 0 1279 2442
Size: 1279 1163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 783 1773 2098 2442
Size: 783 990 325 344
Fragments arranged by size:
990 783 344 325
BstYI r'GATC_y
Cuts at: 0 884 1560 1662 1998 2442
Size: 884 676 102 336 444
Fragments arranged by size:
884 676 444 336 102
Bsu36I CC'TnA_GG
Cuts at: 0 1679 2193 2442
Size: 1679 514 249
Fragments arranged by size:
1679 514 249
BtgI C'CryG_G
Cuts at: 0 1070 1471 2442
Size: 1070 401 971
Fragments arranged by size:
1070 971 401
BtsI GCAGTG_nn'
Cuts at: 0 216 2442
Size: 216 2226
DrdI GACnn_nn'nnGTC
Cuts at: 0 369 2442
Size: 369 2073
EaeI y'GGCC_r
Cuts at: 0 45 738 983 2106 2442
Size: 45 693 245 1123 336
Fragments arranged by size:
1123 693 336 245 45
EarI CTCTTCn'nnn_
Cuts at: 0 132 950 1215 2313 2442
Size: 132 818 265 1098 129
Fragments arranged by size:
1098 818 265 132 129
EcoICRI GAG'CTC
Cuts at: 0 341 2306 2442
Size: 341 1965 136
Fragments arranged by size:
1965 341 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 899 955 1322 1371 1603 1630 1725
Size: 559 340 56 367 49 232 27 95
Cuts at: 1725 1979 2044 2442
Size: 254 65 398
Fragments arranged by size:
559 398 367 340 254 232 95 65
56 49 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1186 1765 2442
Size: 1186 579 677
Fragments arranged by size:
1186 677 579
EcoO109I rG'GnC_Cy
Cuts at: 0 389 456 1270 1342 1343 1372 1675 2112
Size: 389 67 814 72 1 29 303 437
Cuts at: 2112 2442
Size: 330
Fragments arranged by size:
814 437 389 330 303 72 67 29
1
EcoRV GAT'ATC
Cuts at: 0 1418 2442
Size: 1418 1024
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1988 2442
Size: 1988 454
HaeII r_GCGC'y
Cuts at: 0 305 2442
Size: 305 2137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 461 493 1402 1434 1496 1528
Size: 234 32 195 32 909 32 62 32
Cuts at: 1528 1597 1629 2224 2256 2442
Size: 69 32 595 32 186
Fragments arranged by size:
909 595 234 195 186 69 62 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 792 2442
Size: 792 1650
HindIII A'AGCT_T
Cuts at: 0 1011 2442
Size: 1011 1431
KpnI G_GTAC'C
Cuts at: 0 2021 2442
Size: 2021 421
MscI TGG'CCA
Cuts at: 0 47 740 985 2442
Size: 47 693 245 1457
Fragments arranged by size:
1457 693 245 47
MslI CAynn'nnrTG
Cuts at: 0 644 781 2437 2442
Size: 644 137 1656 5
Fragments arranged by size:
1656 644 137 5
MspA1I CmG'CkG
Cuts at: 0 173 933 1165 1261 1818 2175 2270 2442
Size: 173 760 232 96 557 357 95 172
Fragments arranged by size:
760 557 357 232 173 172 96 95
NcoI C'CATG_G
Cuts at: 0 1070 1471 2442
Size: 1070 401 971
Fragments arranged by size:
1070 971 401
NdeI CA'TA_TG
Cuts at: 0 1353 2442
Size: 1353 1089
NspI r_CATG'y
Cuts at: 0 1909 2442
Size: 1909 533
PasI CC'CwG_GG
Cuts at: 0 1399 2442
Size: 1399 1043
PciI A'CATG_T
Cuts at: 0 1905 2442
Size: 1905 537
PflMI CCAn_nnn'nTGG
Cuts at: 0 54 1254 2442
Size: 54 1200 1188
Fragments arranged by size:
1200 1188 54
PfoI T'CCnGG_A
Cuts at: 0 458 1975 2442
Size: 458 1517 467
Fragments arranged by size:
1517 467 458
PpuMI rG'GwC_Cy
Cuts at: 0 389 456 2442
Size: 389 67 1986
Fragments arranged by size:
1986 389 67
PspOMI G'GGCC_C
Cuts at: 0 1342 1674 1820 2111 2387 2442
Size: 1342 332 146 291 276 55
Fragments arranged by size:
1342 332 291 276 146 55
PstI C_TGCA'G
Cuts at: 0 223 491 1118 2442
Size: 223 268 627 1324
Fragments arranged by size:
1324 627 268 223
PvuII CAG'CTG
Cuts at: 0 173 933 1165 1261 2175 2442
Size: 173 760 232 96 914 267
Fragments arranged by size:
914 760 267 232 173 96
SacI G_AGCT'C
Cuts at: 0 343 2308 2442
Size: 343 1965 134
Fragments arranged by size:
1965 343 134
SanDI GG'GwC_CC
Cuts at: 0 456 2442
Size: 456 1986
SapI GCTCTTCn'nnn_
Cuts at: 0 2313 2442
Size: 2313 129
SbfI CC_TGCA'GG
Cuts at: 0 1118 2442
Size: 1118 1324
SexAI A'CCwGG_T
Cuts at: 0 857 2013 2442
Size: 857 1156 429
Fragments arranged by size:
1156 857 429
SfcI C'TryA_G
Cuts at: 0 219 487 1114 2442
Size: 219 268 627 1328
Fragments arranged by size:
1328 627 268 219
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 737 2442
Size: 737 1705
SmaI CCC'GGG
Cuts at: 0 1321 2442
Size: 1321 1121
SmlI C'TyrA_G
Cuts at: 0 1366 2003 2442
Size: 1366 637 439
Fragments arranged by size:
1366 637 439
SspI AAT'ATT
Cuts at: 0 991 2442
Size: 991 1451
StyI C'CwwG_G
Cuts at: 0 1070 1471 1766 2442
Size: 1070 401 295 676
Fragments arranged by size:
1070 676 401 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 537 652 1233 2442
Size: 537 115 581 1209
Fragments arranged by size:
1209 581 537 115
TatI w'GTAC_w
Cuts at: 0 801 895 2442
Size: 801 94 1547
Fragments arranged by size:
1547 801 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1315 1347 1716 1748 2368 2400 2442
Size: 1315 32 369 32 620 32 42
Fragments arranged by size:
1315 620 369 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1510 2442
Size: 1510 932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1024 2442
Size: 1024 1418
XmaI C'CCGG_G
Cuts at: 0 1319 2442
Size: 1319 1123
XmnI GAAnn'nnTTC
Cuts at: 0 1968 2442
Size: 1968 474
ZraI GAC'GTC
Cuts at: 0 1227 2442
Size: 1227 1215
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbsI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII HindIII KpnI MscI MslI MspA1I
NcoI NdeI NspI PasI PciI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI
SfcI SfiI SmaI SmlI SspI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoRI FseI
FspI FspAI HpaI KasI MfeI MluI MmeI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI ScaI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI XbaI XhoI