Restriction map for
pFN21AE9426
(Linear) (Six-base) MAPSORT of: KIEE9426.seq Check: 4968 from: 1 to: 2391
KIEE9426.orf
With 182 enzymes: SgfI *
March 22, 2012 18:27 ..
AclI AA'CG_TT
Cuts at: 0 1316 2391
Size: 1316 1075
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 773 904 2085 2228 2391
Size: 506 267 131 1181 143 163
Fragments arranged by size:
1181 506 267 163 143 131
AfeI AGC'GCT
Cuts at: 0 1641 2391
Size: 1641 750
AflIII A'CryG_T
Cuts at: 0 310 2391
Size: 310 2081
AlwNI CAG_nnn'CTG
Cuts at: 0 1971 2391
Size: 1971 420
ApaLI G'TGCA_C
Cuts at: 0 1325 1575 2391
Size: 1325 250 816
Fragments arranged by size:
1325 816 250
ApoI r'AATT_y
Cuts at: 0 780 1123 1158 2144 2391
Size: 780 343 35 986 247
Fragments arranged by size:
986 780 343 247 35
AseI AT'TA_AT
Cuts at: 0 1261 1714 2391
Size: 1261 453 677
Fragments arranged by size:
1261 677 453
BamHI G'GATC_C
Cuts at: 0 104 2391
Size: 104 2287
BbsI GAAGACnn'nnnn_
Cuts at: 0 2091 2169 2391
Size: 2091 78 222
Fragments arranged by size:
2091 222 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1929 1963 2076 2110 2391
Size: 1929 34 113 34 281
Fragments arranged by size:
1929 281 113 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 315 2391
Size: 315 2076
BclI T'GATC_A
Cuts at: 0 98 2391
Size: 98 2293
Bme1580I G_kGCm'C
Cuts at: 0 1210 1329 1579 2391
Size: 1210 119 250 812
Fragments arranged by size:
1210 812 250 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 88 274 1185 2391
Size: 88 186 911 1206
Fragments arranged by size:
1206 911 186 88
BmtI G_CTAG'C
Cuts at: 0 845 2391
Size: 845 1546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 2391
Size: 108 2283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1029 2391
Size: 1029 1362
BsaBI GATnn'nnATC
Cuts at: 0 97 103 2391
Size: 97 6 2288
Fragments arranged by size:
2288 97 6
BsaWI w'CCGG_w
Cuts at: 0 353 2391
Size: 353 2038
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1438 2391
Size: 1438 953
BseYI C'CCAG_C
Cuts at: 0 1731 2391
Size: 1731 660
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 850 1582 1628 2391
Size: 381 469 732 46 763
Fragments arranged by size:
763 732 469 381 46
BsiEI CG_ry'CG
Cuts at: 0 2257 2391
Size: 2257 134
BsiHKAI G_wGCw'C
Cuts at: 0 1329 1579 2391
Size: 1329 250 812
Fragments arranged by size:
1329 812 250
Bsp1286I G_dGCh'C
Cuts at: 0 1210 1329 1579 2391
Size: 1210 119 250 812
Fragments arranged by size:
1210 812 250 119
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 435 2391
Size: 435 1956
BsrDI GCAATG_nn'
Cuts at: 0 423 1920 2236 2293 2391
Size: 423 1497 316 57 98
Fragments arranged by size:
1497 423 316 98 57
BsrFI r'CCGG_y
Cuts at: 0 1808 2391
Size: 1808 583
BsrGI T'GTAC_A
Cuts at: 0 193 220 2156 2391
Size: 193 27 1936 235
Fragments arranged by size:
1936 235 193 27
BstYI r'GATC_y
Cuts at: 0 104 2391
Size: 104 2287
Bsu36I CC'TnA_GG
Cuts at: 0 25 593 2287 2391
Size: 25 568 1694 104
Fragments arranged by size:
1694 568 104 25
BtsI GCAGTG_nn'
Cuts at: 0 1577 2391
Size: 1577 814
EaeI y'GGCC_r
Cuts at: 0 2254 2391
Size: 2254 137
EagI C'GGCC_G
Cuts at: 0 2254 2391
Size: 2254 137
EarI CTCTTCn'nnn_
Cuts at: 0 194 792 1688 2391
Size: 194 598 896 703
Fragments arranged by size:
896 703 598 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 108 506 773 904 2085 2228 2391
Size: 108 398 267 131 1181 143 163
Fragments arranged by size:
1181 398 267 163 143 131 108
EcoO109I rG'GnC_Cy
Cuts at: 0 805 2391
Size: 805 1586
HaeII r_GCGC'y
Cuts at: 0 1643 2391
Size: 1643 748
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 1294 1326 1712 1744 2391
Size: 68 32 1194 32 386 32 647
Fragments arranged by size:
1194 647 386 68 32 32 32
HincII GTy'rAC
Cuts at: 0 628 1089 2391
Size: 628 461 1302
Fragments arranged by size:
1302 628 461
HindIII A'AGCT_T
Cuts at: 0 988 2189 2391
Size: 988 1201 202
Fragments arranged by size:
1201 988 202
MfeI C'AATT_G
Cuts at: 0 224 2391
Size: 224 2167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 1299 1337 2391
Size: 537 762 38 1054
Fragments arranged by size:
1054 762 537 38
MslI CAynn'nnrTG
Cuts at: 0 881 1429 2391
Size: 881 548 962
Fragments arranged by size:
962 881 548
MspA1I CmG'CkG
Cuts at: 0 1232 1869 2391
Size: 1232 637 522
Fragments arranged by size:
1232 637 522
NdeI CA'TA_TG
Cuts at: 0 1185 2391
Size: 1185 1206
NheI G'CTAG_C
Cuts at: 0 841 2391
Size: 841 1550
NspI r_CATG'y
Cuts at: 0 314 962 2391
Size: 314 648 1429
Fragments arranged by size:
1429 648 314
PciI A'CATG_T
Cuts at: 0 310 2391
Size: 310 2081
PsiI TTA'TAA
Cuts at: 0 1858 2391
Size: 1858 533
PstI C_TGCA'G
Cuts at: 0 157 2391
Size: 157 2234
ScaI AGT'ACT
Cuts at: 0 2343 2391
Size: 2343 48
SfcI C'TryA_G
Cuts at: 0 143 153 1736 2391
Size: 143 10 1583 655
Fragments arranged by size:
1583 655 143 10
SmlI C'TyrA_G
Cuts at: 0 1044 2391
Size: 1044 1347
SpeI A'CTAG_T
Cuts at: 0 538 2391
Size: 538 1853
SspI AAT'ATT
Cuts at: 0 1515 2391
Size: 1515 876
TatI w'GTAC_w
Cuts at: 0 193 220 1109 2156 2341 2391
Size: 193 27 889 1047 185 50
Fragments arranged by size:
1047 889 193 185 50 27
Tth111I GACn'n_nGTC
Cuts at: 0 1093 2391
Size: 1093 1298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 279 2391
Size: 279 2112
XmnI GAAnn'nnTTC
Cuts at: 0 585 2391
Size: 585 1806
Enzymes that do cut:
AclI AcuI AfeI AflIII AlwNI ApaLI ApoI AseI
BamHI BbsI BcgI BciVI BclI Bme1580I BmrI BmtI
BpmI BpuEI BsaBI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BsrGI BstYI Bsu36I
BtsI EaeI EagI EarI Eco57MI EcoO109I HaeII Hin4I
HincII HindIII MfeI MmeI MslI MspA1I NdeI NheI
NspI PciI PsiI PstI ScaI SfcI SmlI SpeI
SspI TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BbvCI BfrBI BglI BglII BlpI
BmgBI BplI Bpu10I BsaI BsaAI BsaHI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NgoMIV NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI StuI StyI
SwaI TaqII TstI XbaI XhoI XmaI ZraI