Restriction map for
pFN21AE9425
(Linear) (Six-base) MAPSORT of: KIEE9425.seq Check: 190 from: 1 to: 3666
KIEE9425.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AccI GT'mk_AC
Cuts at: 0 1001 3666
Size: 1001 2665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1889 2281 3630 3666
Size: 1889 392 1349 36
Fragments arranged by size:
1889 1349 392 36
AflII C'TTAA_G
Cuts at: 0 456 1830 3666
Size: 456 1374 1836
Fragments arranged by size:
1836 1374 456
AflIII A'CryG_T
Cuts at: 0 1926 2369 2777 3666
Size: 1926 443 408 889
Fragments arranged by size:
1926 889 443 408
AgeI A'CCGG_T
Cuts at: 0 223 3666
Size: 223 3443
AhdI GACnn_n'nnGTC
Cuts at: 0 1130 3666
Size: 1130 2536
AleI CACnn'nnGTG
Cuts at: 0 1286 3666
Size: 1286 2380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2653 3666
Size: 2653 1013
AlwNI CAG_nnn'CTG
Cuts at: 0 2259 3666
Size: 2259 1407
ApaI G_GGCC'C
Cuts at: 0 1356 3666
Size: 1356 2310
ApoI r'AATT_y
Cuts at: 0 325 798 939 1692 1998 2109 2833 2947
Size: 325 473 141 753 306 111 724 114
Cuts at: 2947 3666
Size: 719
Fragments arranged by size:
753 724 719 473 325 306 141 114
111
BamHI G'GATC_C
Cuts at: 0 2067 2265 3666
Size: 2067 198 1401
Fragments arranged by size:
2067 1401 198
BanII G_rGCy'C
Cuts at: 0 219 539 787 883 1356 3666
Size: 219 320 248 96 473 2310
Fragments arranged by size:
2310 473 320 248 219 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 590 734 2005 3392 3666
Size: 590 144 1271 1387 274
Fragments arranged by size:
1387 1271 590 274 144
BbvCI CC'TCA_GC
Cuts at: 0 973 1722 3666
Size: 973 749 1944
Fragments arranged by size:
1944 973 749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 360 394 3666
Size: 360 34 3272
Fragments arranged by size:
3272 360 34
BfrBI ATG'CAT
Cuts at: 0 381 619 706 3666
Size: 381 238 87 2960
Fragments arranged by size:
2960 381 238 87
BglII A'GATC_T
Cuts at: 0 3053 3666
Size: 3053 613
Bme1580I G_kGCm'C
Cuts at: 0 1356 3666
Size: 1356 2310
BmgBI CAC'GTC
Cuts at: 0 2372 3666
Size: 2372 1294
BmrI ACTGGGnnnn_n'
Cuts at: 0 44 1489 1638 3666
Size: 44 1445 149 2028
Fragments arranged by size:
2028 1445 149 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1313 3666
Size: 1313 2353
Bpu10I CC'TnA_GC
Cuts at: 0 973 1060 1722 2626 3666
Size: 973 87 662 904 1040
Fragments arranged by size:
1040 973 904 662 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 1345 1470 1876 2125 3666
Size: 348 997 125 406 249 1541
Fragments arranged by size:
1541 997 406 348 249 125
BsaBI GATnn'nnATC
Cuts at: 0 3539 3666
Size: 3539 127
BsaWI w'CCGG_w
Cuts at: 0 223 3083 3666
Size: 223 2860 583
Fragments arranged by size:
2860 583 223
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3196 3226 3666
Size: 3196 30 440
Fragments arranged by size:
3196 440 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2644 3666
Size: 2644 1022
BseYI C'CCAG_C
Cuts at: 0 1089 2849 2992 3666
Size: 1089 1760 143 674
Fragments arranged by size:
1760 1089 674 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1133 2250 3070 3666
Size: 1133 1117 820 596
Fragments arranged by size:
1133 1117 820 596
BsiHKAI G_wGCw'C
Cuts at: 0 539 2148 2632 3666
Size: 539 1609 484 1034
Fragments arranged by size:
1609 1034 539 484
BsmI GAATG_Cn'
Cuts at: 0 957 3166 3666
Size: 957 2209 500
Fragments arranged by size:
2209 957 500
Bsp1286I G_dGCh'C
Cuts at: 0 219 539 787 883 1356 2148 2632 3666
Size: 219 320 248 96 473 792 484 1034
Fragments arranged by size:
1034 792 484 473 320 248 219 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3122 3666
Size: 3122 544
BsrBI CCG'CTC
Cuts at: 0 1086 3666
Size: 1086 2580
BsrDI GCAATG_nn'
Cuts at: 0 2705 2783 3023 3603 3666
Size: 2705 78 240 580 63
Fragments arranged by size:
2705 580 240 78 63
BsrFI r'CCGG_y
Cuts at: 0 223 3666
Size: 223 3443
BsrGI T'GTAC_A
Cuts at: 0 2780 3666
Size: 2780 886
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3172 3666
Size: 3172 494
BstEII G'GTnAC_C
Cuts at: 0 1620 3666
Size: 1620 2046
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1617 1743 3535 3666
Size: 1617 126 1792 131
Fragments arranged by size:
1792 1617 131 126
BstYI r'GATC_y
Cuts at: 0 2067 2265 2334 2964 3053 3666
Size: 2067 198 69 630 89 613
Fragments arranged by size:
2067 630 613 198 89 69
Bsu36I CC'TnA_GG
Cuts at: 0 1190 3666
Size: 1190 2476
BtgI C'CryG_G
Cuts at: 0 1496 3500 3666
Size: 1496 2004 166
Fragments arranged by size:
2004 1496 166
BtsI GCAGTG_nn'
Cuts at: 0 3126 3666
Size: 3126 540
DraI TTT'AAA
Cuts at: 0 1658 1691 1877 3666
Size: 1658 33 186 1789
Fragments arranged by size:
1789 1658 186 33
DraIII CAC_nnn'GTG
Cuts at: 0 102 3666
Size: 102 3564
DrdI GACnn_nn'nnGTC
Cuts at: 0 1481 3666
Size: 1481 2185
EaeI y'GGCC_r
Cuts at: 0 576 1103 1522 3666
Size: 576 527 419 2144
Fragments arranged by size:
2144 576 527 419
EarI CTCTTCn'nnn_
Cuts at: 0 2136 2662 3214 3666
Size: 2136 526 552 452
Fragments arranged by size:
2136 552 526 452
EcoICRI GAG'CTC
Cuts at: 0 537 3666
Size: 537 3129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1313 1889 2281 3630 3666
Size: 1313 576 392 1349 36
Fragments arranged by size:
1349 1313 576 392 36
EcoO109I rG'GnC_Cy
Cuts at: 0 149 1124 1353 1633 2191 3181 3666
Size: 149 975 229 280 558 990 485
Fragments arranged by size:
990 975 558 485 280 229 149
EcoRI G'AATT_C
Cuts at: 0 2947 3666
Size: 2947 719
EcoRV GAT'ATC
Cuts at: 0 1644 3666
Size: 1644 2022
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1590 2222 3666
Size: 1590 632 1444
Fragments arranged by size:
1590 1444 632
FspI TGC'GCA
Cuts at: 0 3118 3666
Size: 3118 548
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 1116 1148 2590 2622 2949 2981
Size: 280 32 804 32 1442 32 327 32
Cuts at: 2981 3532 3564 3666
Size: 551 32 102
Fragments arranged by size:
1442 804 551 327 280 102 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1002 3666
Size: 1002 2664
HindIII A'AGCT_T
Cuts at: 0 744 1871 3666
Size: 744 1127 1795
Fragments arranged by size:
1795 1127 744
MfeI C'AATT_G
Cuts at: 0 276 631 1883 3666
Size: 276 355 1252 1783
Fragments arranged by size:
1783 1252 355 276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 992 1557 1673 2935 3443 3666
Size: 161 831 565 116 1262 508 223
Fragments arranged by size:
1262 831 565 508 223 161 116
MscI TGG'CCA
Cuts at: 0 578 1105 1524 3666
Size: 578 527 419 2142
Fragments arranged by size:
2142 578 527 419
MslI CAynn'nnrTG
Cuts at: 0 1286 3028 3533 3666
Size: 1286 1742 505 133
Fragments arranged by size:
1742 1286 505 133
MspA1I CmG'CkG
Cuts at: 0 977 1109 3666
Size: 977 132 2557
Fragments arranged by size:
2557 977 132
NcoI C'CATG_G
Cuts at: 0 1496 3500 3666
Size: 1496 2004 166
Fragments arranged by size:
2004 1496 166
NdeI CA'TA_TG
Cuts at: 0 2571 3666
Size: 2571 1095
NsiI A_TGCA'T
Cuts at: 0 383 621 708 3666
Size: 383 238 87 2958
Fragments arranged by size:
2958 383 238 87
NspI r_CATG'y
Cuts at: 0 204 385 1930 2781 3666
Size: 204 181 1545 851 885
Fragments arranged by size:
1545 885 851 204 181
PciI A'CATG_T
Cuts at: 0 1926 2777 3666
Size: 1926 851 889
Fragments arranged by size:
1926 889 851
PfoI T'CCnGG_A
Cuts at: 0 2180 3666
Size: 2180 1486
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1705 1737 3666
Size: 1705 32 1929
Fragments arranged by size:
1929 1705 32
PpuMI rG'GwC_Cy
Cuts at: 0 149 1124 1633 2191 3181 3666
Size: 149 975 509 558 990 485
Fragments arranged by size:
990 975 558 509 485 149
PsiI TTA'TAA
Cuts at: 0 923 3666
Size: 923 2743
PspOMI G'GGCC_C
Cuts at: 0 1352 3666
Size: 1352 2314
PvuII CAG'CTG
Cuts at: 0 977 1109 3666
Size: 977 132 2557
Fragments arranged by size:
2557 977 132
SacI G_AGCT'C
Cuts at: 0 539 3666
Size: 539 3127
SalI G'TCGA_C
Cuts at: 0 1000 3666
Size: 1000 2666
SanDI GG'GwC_CC
Cuts at: 0 1124 3181 3666
Size: 1124 2057 485
Fragments arranged by size:
2057 1124 485
SapI GCTCTTCn'nnn_
Cuts at: 0 2136 3666
Size: 2136 1530
SexAI A'CCwGG_T
Cuts at: 0 500 3666
Size: 500 3166
SfcI C'TryA_G
Cuts at: 0 1149 3666
Size: 1149 2517
SmlI C'TyrA_G
Cuts at: 0 363 456 1324 1449 1830 1855 2104 3666
Size: 363 93 868 125 381 25 249 1562
Fragments arranged by size:
1562 868 381 363 249 125 93 25
SspI AAT'ATT
Cuts at: 0 1731 1935 3627 3666
Size: 1731 204 1692 39
Fragments arranged by size:
1731 1692 204 39
StuI AGG'CCT
Cuts at: 0 350 3666
Size: 350 3316
StyI C'CwwG_G
Cuts at: 0 1336 1436 1496 3500 3666
Size: 1336 100 60 2004 166
Fragments arranged by size:
2004 1336 166 100 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2516 3666
Size: 2516 1150
TatI w'GTAC_w
Cuts at: 0 1200 2780 3666
Size: 1200 1580 886
Fragments arranged by size:
1580 1200 886
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 3036 3666
Size: 123 2913 630
Fragments arranged by size:
2913 630 123
XmnI GAAnn'nnTTC
Cuts at: 0 421 821 1547 2771 3666
Size: 421 400 726 1224 895
Fragments arranged by size:
1224 895 726 421 400
Enzymes that do cut:
AccI AcuI AflII AflIII AgeI AhdI AleI AlfI
AlwNI ApaI ApoI BamHI BanII BbsI BbvCI BcgI
BfrBI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI Hin4I HincII HindIII MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PciI PfoI PpiI
PpuMI PsiI PspOMI PvuII SacI SalI SanDI SapI
SexAI SfcI SmlI SspI StuI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AloI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BciVI
BclI BglI BlpI BmtI BplI BsaI BsaAI BsaHI
BsiEI BsiWI BsmBI BspEI BspHI BssHII BssSI BstBI
BstZ17I BtgZI ClaI CspCI EagI EciI EcoNI FseI
FspAI HaeII HpaI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PflMI PmeI
PmlI PshAI PspXI PsrI PstI PvuI RsrII SacII
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI