Restriction map for
pFN21AE9422
(Linear) (Six-base) MAPSORT of: KIEE9422.seq Check: 3915 from: 1 to: 2070
KIEE9422.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 983 995 1139 1338 2070
Size: 252 731 12 144 199 732
Fragments arranged by size:
732 731 252 199 144 12
AhdI GACnn_n'nnGTC
Cuts at: 0 77 2070
Size: 77 1993
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1539 2070
Size: 1539 531
ApaLI G'TGCA_C
Cuts at: 0 1915 2070
Size: 1915 155
ApoI r'AATT_y
Cuts at: 0 189 444 577 744 1361 1593 2070
Size: 189 255 133 167 617 232 477
Fragments arranged by size:
617 477 255 232 189 167 133
AseI AT'TA_AT
Cuts at: 0 377 2070
Size: 377 1693
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 605 638 2070
Size: 605 33 1432
Fragments arranged by size:
1432 605 33
BanI G'GyrC_C
Cuts at: 0 1882 2070
Size: 1882 188
BanII G_rGCy'C
Cuts at: 0 1024 1860 2070
Size: 1024 836 210
Fragments arranged by size:
1024 836 210
BbsI GAAGACnn'nnnn_
Cuts at: 0 1145 2070
Size: 1145 925
BciVI GTATCCnnnnn_n'
Cuts at: 0 606 1648 2070
Size: 606 1042 422
Fragments arranged by size:
1042 606 422
BfrBI ATG'CAT
Cuts at: 0 1334 2070
Size: 1334 736
BglII A'GATC_T
Cuts at: 0 456 2070
Size: 456 1614
BlpI GC'TnA_GC
Cuts at: 0 1690 2070
Size: 1690 380
Bme1580I G_kGCm'C
Cuts at: 0 1919 2070
Size: 1919 151
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1876 2070
Size: 1876 194
BsaI GGTCTCn'nnnn_
Cuts at: 0 1180 2070
Size: 1180 890
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 65 95 1092 1122 2070
Size: 65 30 997 30 948
Fragments arranged by size:
997 948 65 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 994 1868 2070
Size: 994 874 202
Fragments arranged by size:
994 874 202
BseYI C'CCAG_C
Cuts at: 0 1620 2070
Size: 1620 450
BsiHKAI G_wGCw'C
Cuts at: 0 1024 1919 2070
Size: 1024 895 151
Fragments arranged by size:
1024 895 151
BsmI GAATG_Cn'
Cuts at: 0 339 2070
Size: 339 1731
Bsp1286I G_dGCh'C
Cuts at: 0 1024 1860 1919 2070
Size: 1024 836 59 151
Fragments arranged by size:
1024 836 151 59
BsrBI CCG'CTC
Cuts at: 0 15 2070
Size: 15 2055
BsrDI GCAATG_nn'
Cuts at: 0 114 885 1475 1962 2070
Size: 114 771 590 487 108
Fragments arranged by size:
771 590 487 114 108
BstYI r'GATC_y
Cuts at: 0 271 400 456 1892 2070
Size: 271 129 56 1436 178
Fragments arranged by size:
1436 271 178 129 56
BtgI C'CryG_G
Cuts at: 0 1625 2070
Size: 1625 445
BtsI GCAGTG_nn'
Cuts at: 0 1796 2070
Size: 1796 274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 717 752 2070
Size: 717 35 1318
Fragments arranged by size:
1318 717 35
EarI CTCTTCn'nnn_
Cuts at: 0 971 1468 1871 1948 2070
Size: 971 497 403 77 122
Fragments arranged by size:
971 497 403 122 77
EcoICRI GAG'CTC
Cuts at: 0 1022 2070
Size: 1022 1048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 983 995 1139 1338 2070
Size: 252 731 12 144 199 732
Fragments arranged by size:
732 731 252 199 144 12
EcoRV GAT'ATC
Cuts at: 0 707 2070
Size: 707 1363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 622 645 654 677 900 932
Size: 63 32 527 23 9 23 223 32
Cuts at: 932 2070
Size: 1138
Fragments arranged by size:
1138 527 223 63 32 32 23 23
9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1614 2070
Size: 1614 456
MslI CAynn'nnrTG
Cuts at: 0 1521 1555 2070
Size: 1521 34 515
Fragments arranged by size:
1521 515 34
MspA1I CmG'CkG
Cuts at: 0 1148 1205 2070
Size: 1148 57 865
Fragments arranged by size:
1148 865 57
NdeI CA'TA_TG
Cuts at: 0 812 864 1330 2070
Size: 812 52 466 740
Fragments arranged by size:
812 740 466 52
NsiI A_TGCA'T
Cuts at: 0 1336 2070
Size: 1336 734
NspI r_CATG'y
Cuts at: 0 1736 2070
Size: 1736 334
PsiI TTA'TAA
Cuts at: 0 905 1271 2070
Size: 905 366 799
Fragments arranged by size:
905 799 366
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 648 680 2070
Size: 648 32 1390
Fragments arranged by size:
1390 648 32
PstI C_TGCA'G
Cuts at: 0 1803 2070
Size: 1803 267
PvuII CAG'CTG
Cuts at: 0 1148 2070
Size: 1148 922
SacI G_AGCT'C
Cuts at: 0 1024 2070
Size: 1024 1046
SapI GCTCTTCn'nnn_
Cuts at: 0 1468 1871 2070
Size: 1468 403 199
Fragments arranged by size:
1468 403 199
SfcI C'TryA_G
Cuts at: 0 438 1401 1799 2070
Size: 438 963 398 271
Fragments arranged by size:
963 438 398 271
SphI G_CATG'C
Cuts at: 0 1736 2070
Size: 1736 334
SspI AAT'ATT
Cuts at: 0 375 494 1275 2023 2070
Size: 375 119 781 748 47
Fragments arranged by size:
781 748 375 119 47
StyI C'CwwG_G
Cuts at: 0 545 2070
Size: 545 1525
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1047 1549 2070
Size: 1047 502 521
Fragments arranged by size:
1047 521 502
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 1092 1124 2070
Size: 65 32 995 32 946
Fragments arranged by size:
995 946 65 32 32
XbaI T'CTAG_A
Cuts at: 0 2002 2070
Size: 2002 68
Enzymes that do cut:
AcuI AhdI AlfI ApaLI ApoI AseI BaeI BanI
BanII BbsI BciVI BfrBI BglII BlpI Bme1580I BplI
BsaI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrBI
BsrDI BstYI BtgI BtsI CspCI EarI EcoICRI Eco57MI
EcoRV Hin4I MmeI MslI MspA1I NdeI NsiI NspI
PsiI PsrI PstI PvuII SacI SapI SfcI SphI
SspI StyI TaqII TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI AscI AsiSI AvaI
AvrII BamHI BbeI BbvCI BcgI BclI BglI BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI
TatI Tth111I XcmI XhoI XmaI XmnI ZraI