Restriction map for
pFN21AE9420
(Linear) (Six-base) MAPSORT of: KIEE9420.seq Check: 5090 from: 1 to: 1011
KIEE9420.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AccI GT'mk_AC
Cuts at: 0 821 1011
Size: 821 190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1011
Size: 47 964
AfeI AGC'GCT
Cuts at: 0 191 1011
Size: 191 820
AflIII A'CryG_T
Cuts at: 0 239 1011
Size: 239 772
AleI CACnn'nnGTG
Cuts at: 0 517 1011
Size: 517 494
AlwNI CAG_nnn'CTG
Cuts at: 0 546 582 1011
Size: 546 36 429
Fragments arranged by size:
546 429 36
ApoI r'AATT_y
Cuts at: 0 592 1011
Size: 592 419
BanI G'GyrC_C
Cuts at: 0 480 1011
Size: 480 531
BbsI GAAGACnn'nnnn_
Cuts at: 0 3 963 1011
Size: 3 960 48
Fragments arranged by size:
960 48 3
BbvCI CC'TCA_GC
Cuts at: 0 268 1011
Size: 268 743
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 479 513 1011
Size: 479 34 498
Fragments arranged by size:
498 479 34
BclI T'GATC_A
Cuts at: 0 644 1011
Size: 644 367
BglII A'GATC_T
Cuts at: 0 662 1011
Size: 662 349
BlpI GC'TnA_GC
Cuts at: 0 535 689 1011
Size: 535 154 322
Fragments arranged by size:
535 322 154
Bme1580I G_kGCm'C
Cuts at: 0 485 1011
Size: 485 526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 467 1011
Size: 467 544
Bpu10I CC'TnA_GC
Cuts at: 0 268 1011
Size: 268 743
BseYI C'CCAG_C
Cuts at: 0 721 1011
Size: 721 290
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 310 1011
Size: 143 167 701
Fragments arranged by size:
701 167 143
BsiHKAI G_wGCw'C
Cuts at: 0 35 1011
Size: 35 976
BsmI GAATG_Cn'
Cuts at: 0 43 1011
Size: 43 968
Bsp1286I G_dGCh'C
Cuts at: 0 35 485 1011
Size: 35 450 526
Fragments arranged by size:
526 450 35
BsrDI GCAATG_nn'
Cuts at: 0 764 1011
Size: 764 247
BstXI CCAn_nnnn'nTGG
Cuts at: 0 61 759 1011
Size: 61 698 252
Fragments arranged by size:
698 252 61
BstYI r'GATC_y
Cuts at: 0 662 1011
Size: 662 349
DrdI GACnn_nn'nnGTC
Cuts at: 0 817 1011
Size: 817 194
EarI CTCTTCn'nnn_
Cuts at: 0 23 352 545 1011
Size: 23 329 193 466
Fragments arranged by size:
466 329 193 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 1011
Size: 47 964
HaeII r_GCGC'y
Cuts at: 0 193 1011
Size: 193 818
HincII GTy'rAC
Cuts at: 0 822 1011
Size: 822 189
MslI CAynn'nnrTG
Cuts at: 0 517 769 1011
Size: 517 252 242
Fragments arranged by size:
517 252 242
MspA1I CmG'CkG
Cuts at: 0 579 1011
Size: 579 432
NspI r_CATG'y
Cuts at: 0 135 243 1011
Size: 135 108 768
Fragments arranged by size:
768 135 108
PasI CC'CwG_GG
Cuts at: 0 759 1011
Size: 759 252
PciI A'CATG_T
Cuts at: 0 239 1011
Size: 239 772
PflMI CCAn_nnn'nTGG
Cuts at: 0 546 1011
Size: 546 465
PvuII CAG'CTG
Cuts at: 0 579 1011
Size: 579 432
SalI G'TCGA_C
Cuts at: 0 820 1011
Size: 820 191
SapI GCTCTTCn'nnn_
Cuts at: 0 23 1011
Size: 23 988
SexAI A'CCwGG_T
Cuts at: 0 53 1011
Size: 53 958
SphI G_CATG'C
Cuts at: 0 135 1011
Size: 135 876
StyI C'CwwG_G
Cuts at: 0 18 1011
Size: 18 993
TatI w'GTAC_w
Cuts at: 0 81 1011
Size: 81 930
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 464 496 1011
Size: 464 32 515
Fragments arranged by size:
515 464 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 282 813 1011
Size: 282 531 198
Fragments arranged by size:
531 282 198
XmnI GAAnn'nnTTC
Cuts at: 0 970 1011
Size: 970 41
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlwNI ApoI BanI
BbsI BbvCI BcgI BclI BglII BlpI Bme1580I BplI
Bpu10I BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BstXI
BstYI DrdI EarI Eco57MI HaeII HincII MslI MspA1I
NspI PasI PciI PflMI PvuII SalI SapI SexAI
SphI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BciVI BfrBI BglI BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI