Restriction map for
pFN21AE9418
(Linear) (Six-base) MAPSORT of: KIEE9418.seq Check: 3279 from: 1 to: 1134
KIEE9418.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AccI GT'mk_AC
Cuts at: 0 329 1134
Size: 329 805
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1134
Size: 994 140
AflIII A'CryG_T
Cuts at: 0 648 1134
Size: 648 486
AleI CACnn'nnGTG
Cuts at: 0 98 1134
Size: 98 1036
AlwNI CAG_nnn'CTG
Cuts at: 0 216 1134
Size: 216 918
ApaI G_GGCC'C
Cuts at: 0 831 1134
Size: 831 303
ApoI r'AATT_y
Cuts at: 0 14 1134
Size: 14 1120
BanI G'GyrC_C
Cuts at: 0 846 1134
Size: 846 288
BanII G_rGCy'C
Cuts at: 0 689 831 1134
Size: 689 142 303
Fragments arranged by size:
689 303 142
BbsI GAAGACnn'nnnn_
Cuts at: 0 279 1134
Size: 279 855
BglI GCCn_nnn'nGGC
Cuts at: 0 241 1134
Size: 241 893
BglII A'GATC_T
Cuts at: 0 800 1134
Size: 800 334
Bme1580I G_kGCm'C
Cuts at: 0 831 1134
Size: 831 303
BmgBI CAC'GTC
Cuts at: 0 357 1134
Size: 357 777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 749 1012 1134
Size: 749 263 122
Fragments arranged by size:
749 263 122
Bpu10I CC'TnA_GC
Cuts at: 0 838 1134
Size: 838 296
BsaI GGTCTCn'nnnn_
Cuts at: 0 258 1134
Size: 258 876
BsaHI Gr'CG_yC
Cuts at: 0 146 1134
Size: 146 988
BseYI C'CCAG_C
Cuts at: 0 761 1134
Size: 761 373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 817 1134
Size: 564 253 317
Fragments arranged by size:
564 317 253
BsiHKAI G_wGCw'C
Cuts at: 0 1115 1134
Size: 1115 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 157 1134
Size: 157 977
Bsp1286I G_dGCh'C
Cuts at: 0 689 831 1115 1134
Size: 689 142 284 19
Fragments arranged by size:
689 284 142 19
BsrBI CCG'CTC
Cuts at: 0 510 1134
Size: 510 624
BsrDI GCAATG_nn'
Cuts at: 0 230 1134
Size: 230 904
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 1134
Size: 216 918
BstEII G'GTnAC_C
Cuts at: 0 100 1134
Size: 100 1034
BstXI CCAn_nnnn'nTGG
Cuts at: 0 37 1134
Size: 37 1097
BstYI r'GATC_y
Cuts at: 0 800 1134
Size: 800 334
BstZ17I GTA'TAC
Cuts at: 0 330 1134
Size: 330 804
BtsI GCAGTG_nn'
Cuts at: 0 206 386 1134
Size: 206 180 748
Fragments arranged by size:
748 206 180
EaeI y'GGCC_r
Cuts at: 0 446 494 1134
Size: 446 48 640
Fragments arranged by size:
640 446 48
EarI CTCTTCn'nnn_
Cuts at: 0 532 694 1134
Size: 532 162 440
Fragments arranged by size:
532 440 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1134
Size: 994 140
EcoO109I rG'GnC_Cy
Cuts at: 0 213 594 828 1134
Size: 213 381 234 306
Fragments arranged by size:
381 306 234 213
EcoRV GAT'ATC
Cuts at: 0 68 1134
Size: 68 1066
HaeII r_GCGC'y
Cuts at: 0 926 1134
Size: 926 208
HindIII A'AGCT_T
Cuts at: 0 676 1134
Size: 676 458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1134
Size: 1023 111
MscI TGG'CCA
Cuts at: 0 448 496 1134
Size: 448 48 638
Fragments arranged by size:
638 448 48
MslI CAynn'nnrTG
Cuts at: 0 98 181 199 1134
Size: 98 83 18 935
Fragments arranged by size:
935 98 83 18
MspA1I CmG'CkG
Cuts at: 0 1048 1134
Size: 1048 86
NdeI CA'TA_TG
Cuts at: 0 182 1134
Size: 182 952
NspI r_CATG'y
Cuts at: 0 652 662 1090 1134
Size: 652 10 428 44
Fragments arranged by size:
652 428 44 10
PasI CC'CwG_GG
Cuts at: 0 1106 1134
Size: 1106 28
PciI A'CATG_T
Cuts at: 0 648 1134
Size: 648 486
PsiI TTA'TAA
Cuts at: 0 913 1134
Size: 913 221
PspOMI G'GGCC_C
Cuts at: 0 827 1134
Size: 827 307
PvuII CAG'CTG
Cuts at: 0 1048 1134
Size: 1048 86
SapI GCTCTTCn'nnn_
Cuts at: 0 694 1134
Size: 694 440
SexAI A'CCwGG_T
Cuts at: 0 125 341 1134
Size: 125 216 793
Fragments arranged by size:
793 216 125
StyI C'CwwG_G
Cuts at: 0 239 1134
Size: 239 895
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 1134
Size: 32 32 1070
Fragments arranged by size:
1070 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 1134
Size: 246 888
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaI ApoI BanI
BanII BbsI BglI BglII Bme1580I BmgBI BplI Bpu10I
BsaI BsaHI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrBI
BsrDI BstAPI BstEII BstXI BstYI BstZ17I BtsI EaeI
EarI Eco57MI EcoO109I EcoRV HaeII HindIII MmeI MscI
MslI MspA1I NdeI NspI PasI PciI PsiI PspOMI
PvuII SapI SexAI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI BmrI BmtI BpmI BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstBI Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI Hin4I HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI ZraI