Restriction map for
pFN21AE9415
(Linear) (Six-base) MAPSORT of: KIEE9415.seq Check: 388 from: 1 to: 1086
KIEE9415.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AccI GT'mk_AC
Cuts at: 0 926 1086
Size: 926 160
AhdI GACnn_n'nnGTC
Cuts at: 0 419 1086
Size: 419 667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 670 739 1086
Size: 670 69 347
Fragments arranged by size:
670 347 69
ApaLI G'TGCA_C
Cuts at: 0 553 1086
Size: 553 533
AvrII C'CTAG_G
Cuts at: 0 588 1086
Size: 588 498
BanI G'GyrC_C
Cuts at: 0 402 758 798 865 1086
Size: 402 356 40 67 221
Fragments arranged by size:
402 356 221 67 40
BbeI G_GCGC'C
Cuts at: 0 762 802 869 1086
Size: 762 40 67 217
Fragments arranged by size:
762 217 67 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 702 1086
Size: 702 384
BclI T'GATC_A
Cuts at: 0 968 1086
Size: 968 118
Bme1580I G_kGCm'C
Cuts at: 0 407 557 859 1058 1086
Size: 407 150 302 199 28
Fragments arranged by size:
407 302 199 150 28
BmgBI CAC'GTC
Cuts at: 0 228 1086
Size: 228 858
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 1086
Size: 186 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 281 1086
Size: 181 100 805
Fragments arranged by size:
805 181 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 161 1076 1086
Size: 161 915 10
Fragments arranged by size:
915 161 10
BsaAI yAC'GTr
Cuts at: 0 678 735 1086
Size: 678 57 351
Fragments arranged by size:
678 351 57
BsaHI Gr'CG_yC
Cuts at: 0 759 799 866 1086
Size: 759 40 67 220
Fragments arranged by size:
759 220 67 40
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 182 212 1086
Size: 182 30 874
Fragments arranged by size:
874 182 30
BseYI C'CCAG_C
Cuts at: 0 184 612 852 1075 1086
Size: 184 428 240 223 11
Fragments arranged by size:
428 240 223 184 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1086
Size: 709 377
BsiEI CG_ry'CG
Cuts at: 0 53 1086
Size: 53 1033
BsiHKAI G_wGCw'C
Cuts at: 0 233 557 1086
Size: 233 324 529
Fragments arranged by size:
529 324 233
Bsp1286I G_dGCh'C
Cuts at: 0 233 407 557 859 1058 1086
Size: 233 174 150 302 199 28
Fragments arranged by size:
302 233 199 174 150 28
BsrBI CCG'CTC
Cuts at: 0 61 1086
Size: 61 1025
BsrFI r'CCGG_y
Cuts at: 0 136 1086
Size: 136 950
BssHII G'CGCG_C
Cuts at: 0 636 667 1011 1086
Size: 636 31 344 75
Fragments arranged by size:
636 344 75 31
BssSI C'ACGA_G
Cuts at: 0 442 1086
Size: 442 644
BstAPI GCAn_nnn'nTGC
Cuts at: 0 561 1086
Size: 561 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 1086
Size: 489 597
BstYI r'GATC_y
Cuts at: 0 1004 1086
Size: 1004 82
BtgI C'CryG_G
Cuts at: 0 220 1086
Size: 220 866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 273 1086
Size: 273 813
BtsI GCAGTG_nn'
Cuts at: 0 789 1086
Size: 789 297
DraIII CAC_nnn'GTG
Cuts at: 0 387 1086
Size: 387 699
DrdI GACnn_nn'nnGTC
Cuts at: 0 922 1086
Size: 922 164
EaeI y'GGCC_r
Cuts at: 0 50 71 341 479 1086
Size: 50 21 270 138 607
Fragments arranged by size:
607 270 138 50 21
EagI C'GGCC_G
Cuts at: 0 50 1086
Size: 50 1036
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 158 681 1086
Size: 158 523 405
Fragments arranged by size:
523 405 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 281 1086
Size: 181 100 805
Fragments arranged by size:
805 181 100
EcoO109I rG'GnC_Cy
Cuts at: 0 109 915 1086
Size: 109 806 171
Fragments arranged by size:
806 171 109
FspI TGC'GCA
Cuts at: 0 310 739 1086
Size: 310 429 347
Fragments arranged by size:
429 347 310
FspAI rTGC'GCAy
Cuts at: 0 739 1086
Size: 739 347
HaeII r_GCGC'y
Cuts at: 0 14 286 307 762 802 869 1086
Size: 14 272 21 455 40 67 217
Fragments arranged by size:
455 272 217 67 40 21 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 405 437 1086
Size: 405 32 649
Fragments arranged by size:
649 405 32
KasI G'GCGC_C
Cuts at: 0 758 798 865 1086
Size: 758 40 67 221
Fragments arranged by size:
758 221 67 40
MscI TGG'CCA
Cuts at: 0 343 481 1086
Size: 343 138 605
Fragments arranged by size:
605 343 138
MslI CAynn'nnrTG
Cuts at: 0 316 322 487 1086
Size: 316 6 165 599
Fragments arranged by size:
599 316 165 6
MspA1I CmG'CkG
Cuts at: 0 177 211 349 663 872 1086
Size: 177 34 138 314 209 214
Fragments arranged by size:
314 214 209 177 138 34
NaeI GCC'GGC
Cuts at: 0 138 1086
Size: 138 948
NarI GG'CG_CC
Cuts at: 0 759 799 866 1086
Size: 759 40 67 220
Fragments arranged by size:
759 220 67 40
NgoMIV G'CCGG_C
Cuts at: 0 136 1086
Size: 136 950
PflMI CCAn_nnn'nTGG
Cuts at: 0 39 399 1086
Size: 39 360 687
Fragments arranged by size:
687 360 39
PmlI CAC'GTG
Cuts at: 0 678 1086
Size: 678 408
PpuMI rG'GwC_Cy
Cuts at: 0 109 915 1086
Size: 109 806 171
Fragments arranged by size:
806 171 109
PstI C_TGCA'G
Cuts at: 0 649 785 1086
Size: 649 136 301
Fragments arranged by size:
649 301 136
PvuII CAG'CTG
Cuts at: 0 177 872 1086
Size: 177 695 214
Fragments arranged by size:
695 214 177
SanDI GG'GwC_CC
Cuts at: 0 109 1086
Size: 109 977
ScaI AGT'ACT
Cuts at: 0 397 1086
Size: 397 689
SfcI C'TryA_G
Cuts at: 0 645 781 1086
Size: 645 136 305
Fragments arranged by size:
645 305 136
SfoI GGC'GCC
Cuts at: 0 760 800 867 1086
Size: 760 40 67 219
Fragments arranged by size:
760 219 67 40
StuI AGG'CCT
Cuts at: 0 593 1086
Size: 593 493
StyI C'CwwG_G
Cuts at: 0 588 1086
Size: 588 498
TatI w'GTAC_w
Cuts at: 0 395 1086
Size: 395 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 182 214 1086
Size: 182 32 872
Fragments arranged by size:
872 182 32
Tth111I GACn'n_nGTC
Cuts at: 0 582 1086
Size: 582 504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 828 894 1086
Size: 324 504 66 192
Fragments arranged by size:
504 324 192 66
Enzymes that do cut:
AccI AhdI AlfI ApaLI AvrII BanI BbeI BbsI
BclI Bme1580I BmgBI BplI BpmI BsaI BsaAI BsaHI
BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI
BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EciI Eco57MI EcoO109I FspI
FspAI HaeII Hin4I KasI MscI MslI MspA1I NaeI
NarI NgoMIV PflMI PmlI PpuMI PstI PvuII SanDI
ScaI SfcI SfoI StuI StyI TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BanII BbvCI BcgI BciVI
BfrBI BglI BglII BlpI BmrI BmtI Bpu10I BpuEI
BsaBI BsaWI BseRI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI EarI EcoICRI EcoNI EcoRI EcoRV FalI
FseI HincII HindIII HpaI KpnI MfeI MluI MmeI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII XbaI XhoI
XmaI XmnI ZraI