Restriction map for
pFN21AE9413
(Linear) (Six-base) MAPSORT of: KIEE9413.seq Check: 7816 from: 1 to: 1116
KIEE9413.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 928 1116
Size: 928 188
AccI GT'mk_AC
Cuts at: 0 854 968 1085 1116
Size: 854 114 117 31
Fragments arranged by size:
854 117 114 31
AfeI AGC'GCT
Cuts at: 0 883 1116
Size: 883 233
AflIII A'CryG_T
Cuts at: 0 644 1088 1116
Size: 644 444 28
Fragments arranged by size:
644 444 28
AhdI GACnn_n'nnGTC
Cuts at: 0 72 1116
Size: 72 1044
AleI CACnn'nnGTG
Cuts at: 0 595 1116
Size: 595 521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1027 1116
Size: 1027 89
AlwNI CAG_nnn'CTG
Cuts at: 0 920 1116
Size: 920 196
ApaI G_GGCC'C
Cuts at: 0 494 537 1116
Size: 494 43 579
Fragments arranged by size:
579 494 43
ApaLI G'TGCA_C
Cuts at: 0 685 1116
Size: 685 431
ApoI r'AATT_y
Cuts at: 0 988 1116
Size: 988 128
AvaI C'yCGr_G
Cuts at: 0 69 1116
Size: 69 1047
BamHI G'GATC_C
Cuts at: 0 1055 1116
Size: 1055 61
BanI G'GyrC_C
Cuts at: 0 522 740 1116
Size: 522 218 376
Fragments arranged by size:
522 376 218
BanII G_rGCy'C
Cuts at: 0 494 537 566 1116
Size: 494 43 29 550
Fragments arranged by size:
550 494 43 29
BbeI G_GCGC'C
Cuts at: 0 744 1116
Size: 744 372
BbsI GAAGACnn'nnnn_
Cuts at: 0 945 1116
Size: 945 171
BclI T'GATC_A
Cuts at: 0 449 1116
Size: 449 667
BglI GCCn_nnn'nGGC
Cuts at: 0 226 1116
Size: 226 890
Bme1580I G_kGCm'C
Cuts at: 0 494 537 689 1116
Size: 494 43 152 427
Fragments arranged by size:
494 427 152 43
BmgBI CAC'GTC
Cuts at: 0 17 1116
Size: 17 1099
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 1116
Size: 75 1041
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 996 1116
Size: 376 620 120
Fragments arranged by size:
620 376 120
Bpu10I CC'TnA_GC
Cuts at: 0 1049 1116
Size: 1049 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 795 928 1116
Size: 43 752 133 188
Fragments arranged by size:
752 188 133 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 465 1116
Size: 465 651
BsaAI yAC'GTr
Cuts at: 0 585 1089 1116
Size: 585 504 27
Fragments arranged by size:
585 504 27
BsaHI Gr'CG_yC
Cuts at: 0 626 741 1116
Size: 626 115 375
Fragments arranged by size:
626 375 115
BseYI C'CCAG_C
Cuts at: 0 549 1116
Size: 549 567
BsiHKAI G_wGCw'C
Cuts at: 0 566 689 1116
Size: 566 123 427
Fragments arranged by size:
566 427 123
Bsp1286I G_dGCh'C
Cuts at: 0 494 537 566 689 1116
Size: 494 43 29 123 427
Fragments arranged by size:
494 427 123 43 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 218 719 928 1116
Size: 47 171 501 209 188
Fragments arranged by size:
501 209 188 171 47
BsrBI CCG'CTC
Cuts at: 0 1053 1116
Size: 1053 63
BsrDI GCAATG_nn'
Cuts at: 0 1101 1116
Size: 1101 15
BsrFI r'CCGG_y
Cuts at: 0 186 525 1116
Size: 186 339 591
Fragments arranged by size:
591 339 186
BstEII G'GTnAC_C
Cuts at: 0 588 1116
Size: 588 528
BstYI r'GATC_y
Cuts at: 0 101 1055 1116
Size: 101 954 61
Fragments arranged by size:
954 101 61
Bsu36I CC'TnA_GG
Cuts at: 0 97 1116
Size: 97 1019
BtgI C'CryG_G
Cuts at: 0 134 1116
Size: 134 982
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 438 468 1116
Size: 438 30 648
Fragments arranged by size:
648 438 30
EaeI y'GGCC_r
Cuts at: 0 110 200 218 365 602 1116
Size: 110 90 18 147 237 514
Fragments arranged by size:
514 237 147 110 90 18
EarI CTCTTCn'nnn_
Cuts at: 0 820 1116
Size: 820 296
EcoICRI GAG'CTC
Cuts at: 0 564 1116
Size: 564 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 996 1116
Size: 376 620 120
Fragments arranged by size:
620 376 120
EcoO109I rG'GnC_Cy
Cuts at: 0 490 491 534 1116
Size: 490 1 43 582
Fragments arranged by size:
582 490 43 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 969 1116
Size: 969 147
HaeII r_GCGC'y
Cuts at: 0 417 744 885 1116
Size: 417 327 141 231
Fragments arranged by size:
417 327 231 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 1116
Size: 58 32 1026
Fragments arranged by size:
1026 58 32
KasI G'GCGC_C
Cuts at: 0 740 1116
Size: 740 376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 677 1116
Size: 260 417 439
Fragments arranged by size:
439 417 260
MscI TGG'CCA
Cuts at: 0 112 220 367 1116
Size: 112 108 147 749
Fragments arranged by size:
749 147 112 108
MslI CAynn'nnrTG
Cuts at: 0 118 172 289 385 595 1116
Size: 118 54 117 96 210 521
Fragments arranged by size:
521 210 118 117 96 54
MspA1I CmG'CkG
Cuts at: 0 571 750 1116
Size: 571 179 366
Fragments arranged by size:
571 366 179
NaeI GCC'GGC
Cuts at: 0 188 527 1116
Size: 188 339 589
Fragments arranged by size:
589 339 188
NarI GG'CG_CC
Cuts at: 0 741 1116
Size: 741 375
NgoMIV G'CCGG_C
Cuts at: 0 186 525 1116
Size: 186 339 591
Fragments arranged by size:
591 339 186
NspI r_CATG'y
Cuts at: 0 264 648 1116
Size: 264 384 468
Fragments arranged by size:
468 384 264
PciI A'CATG_T
Cuts at: 0 644 1116
Size: 644 472
PfoI T'CCnGG_A
Cuts at: 0 353 1116
Size: 353 763
PspOMI G'GGCC_C
Cuts at: 0 490 533 1116
Size: 490 43 583
Fragments arranged by size:
583 490 43
PstI C_TGCA'G
Cuts at: 0 749 1116
Size: 749 367
RsrII CG'GwC_CG
Cuts at: 0 663 1116
Size: 663 453
SacI G_AGCT'C
Cuts at: 0 566 1116
Size: 566 550
SfcI C'TryA_G
Cuts at: 0 745 855 1116
Size: 745 110 261
Fragments arranged by size:
745 261 110
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 226 1116
Size: 226 890
SfoI GGC'GCC
Cuts at: 0 742 1116
Size: 742 374
SmlI C'TyrA_G
Cuts at: 0 22 774 943 1116
Size: 22 752 169 173
Fragments arranged by size:
752 173 169 22
SphI G_CATG'C
Cuts at: 0 264 1116
Size: 264 852
StuI AGG'CCT
Cuts at: 0 793 1000 1116
Size: 793 207 116
Fragments arranged by size:
793 207 116
StyI C'CwwG_G
Cuts at: 0 107 443 593 788 1116
Size: 107 336 150 195 328
Fragments arranged by size:
336 328 195 150 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 456 1116
Size: 94 362 660
Fragments arranged by size:
660 362 94
Enzymes that do cut:
AarI AccI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI
BbsI BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BseYI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstEII BstYI Bsu36I BtgI BtgZI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PspOMI PstI RsrII SacI SfcI
SfiI SfoI SmlI SphI StuI StyI TaqII
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AgeI AloI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BfrBI
BglII BlpI BmtI BplI BsaBI BsaWI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI