Restriction map for
pFN21AE9404
(Linear) (Six-base) MAPSORT of: KIEE9404.seq Check: 6418 from: 1 to: 1041
KIEE9404.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
Acc65I G'GTAC_C
Cuts at: 0 863 1041
Size: 863 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1041
Size: 728 313
AleI CACnn'nnGTG
Cuts at: 0 829 1041
Size: 829 212
AvaI C'yCGr_G
Cuts at: 0 770 1041
Size: 770 271
BanI G'GyrC_C
Cuts at: 0 15 129 622 781 863 1016 1041
Size: 15 114 493 159 82 153 25
Fragments arranged by size:
493 159 153 114 82 25 15
BanII G_rGCy'C
Cuts at: 0 251 1041
Size: 251 790
BbsI GAAGACnn'nnnn_
Cuts at: 0 375 1041
Size: 375 666
BclI T'GATC_A
Cuts at: 0 806 1041
Size: 806 235
BglI GCCn_nnn'nGGC
Cuts at: 0 29 190 335 499 1041
Size: 29 161 145 164 542
Fragments arranged by size:
542 164 161 145 29
BlpI GC'TnA_GC
Cuts at: 0 556 1041
Size: 556 485
Bme1580I G_kGCm'C
Cuts at: 0 34 134 625 784 1041
Size: 34 100 491 159 257
Fragments arranged by size:
491 257 159 100 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 601 738 1041
Size: 306 295 137 303
Fragments arranged by size:
306 303 295 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 1041
Size: 150 891
BsaAI yAC'GTr
Cuts at: 0 819 1041
Size: 819 222
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 309 598 1041
Size: 292 17 289 443
Fragments arranged by size:
443 292 289 17
BseYI C'CCAG_C
Cuts at: 0 133 338 345 575 626 1041
Size: 133 205 7 230 51 415
Fragments arranged by size:
415 230 205 133 51 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 415 675 1041
Size: 378 37 260 366
Fragments arranged by size:
378 366 260 37
BsiHKAI G_wGCw'C
Cuts at: 0 826 1041
Size: 826 215
BsmI GAATG_Cn'
Cuts at: 0 365 1041
Size: 365 676
Bsp1286I G_dGCh'C
Cuts at: 0 34 134 251 625 784 826 1041
Size: 34 100 117 374 159 42 215
Fragments arranged by size:
374 215 159 117 100 42 34
BspHI T'CATG_A
Cuts at: 0 923 1041
Size: 923 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 968 1041
Size: 266 702 73
Fragments arranged by size:
702 266 73
BsrDI GCAATG_nn'
Cuts at: 0 701 1041
Size: 701 340
BsrFI r'CCGG_y
Cuts at: 0 45 971 1041
Size: 45 926 70
Fragments arranged by size:
926 70 45
BstEII G'GTnAC_C
Cuts at: 0 213 1041
Size: 213 828
BstXI CCAn_nnnn'nTGG
Cuts at: 0 330 1041
Size: 330 711
BstYI r'GATC_y
Cuts at: 0 722 1041
Size: 722 319
Bsu36I CC'TnA_GG
Cuts at: 0 124 1041
Size: 124 917
BtgI C'CryG_G
Cuts at: 0 483 1041
Size: 483 558
BtsI GCAGTG_nn'
Cuts at: 0 457 509 1041
Size: 457 52 532
Fragments arranged by size:
532 457 52
DraIII CAC_nnn'GTG
Cuts at: 0 801 1041
Size: 801 240
EaeI y'GGCC_r
Cuts at: 0 43 1041
Size: 43 998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 601 728 738 1041
Size: 306 295 127 10 303
Fragments arranged by size:
306 303 295 127 10
EcoO109I rG'GnC_Cy
Cuts at: 0 120 1041
Size: 120 921
FseI GG_CCGG'CC
Cuts at: 0 49 1041
Size: 49 992
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 626 658 1041
Size: 626 32 383
Fragments arranged by size:
626 383 32
KpnI G_GTAC'C
Cuts at: 0 867 1041
Size: 867 174
MslI CAynn'nnrTG
Cuts at: 0 829 1041
Size: 829 212
MspA1I CmG'CkG
Cuts at: 0 579 978 1041
Size: 579 399 63
Fragments arranged by size:
579 399 63
NaeI GCC'GGC
Cuts at: 0 47 1041
Size: 47 994
NcoI C'CATG_G
Cuts at: 0 483 1041
Size: 483 558
NgoMIV G'CCGG_C
Cuts at: 0 45 1041
Size: 45 996
NspI r_CATG'y
Cuts at: 0 84 312 1041
Size: 84 228 729
Fragments arranged by size:
729 228 84
PfoI T'CCnGG_A
Cuts at: 0 1009 1041
Size: 1009 32
PpuMI rG'GwC_Cy
Cuts at: 0 120 1041
Size: 120 921
PstI C_TGCA'G
Cuts at: 0 183 1041
Size: 183 858
PvuII CAG'CTG
Cuts at: 0 579 978 1041
Size: 579 399 63
Fragments arranged by size:
579 399 63
SexAI A'CCwGG_T
Cuts at: 0 859 1041
Size: 859 182
SfcI C'TryA_G
Cuts at: 0 35 179 1041
Size: 35 144 862
Fragments arranged by size:
862 144 35
SmaI CCC'GGG
Cuts at: 0 772 1041
Size: 772 269
SmlI C'TyrA_G
Cuts at: 0 165 1041
Size: 165 876
SphI G_CATG'C
Cuts at: 0 84 1041
Size: 84 957
StyI C'CwwG_G
Cuts at: 0 107 483 1041
Size: 107 376 558
Fragments arranged by size:
558 376 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 322 640 1041
Size: 322 318 401
Fragments arranged by size:
401 322 318
TatI w'GTAC_w
Cuts at: 0 146 1041
Size: 146 895
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1041
Size: 991 32 18
Fragments arranged by size:
991 32 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 634 877 1041
Size: 634 243 164
Fragments arranged by size:
634 243 164
XmaI C'CCGG_G
Cuts at: 0 770 1041
Size: 770 271
Enzymes that do cut:
Acc65I AcuI AleI AvaI BanI BanII BbsI BclI
BglI BlpI Bme1580I BpmI BpuEI BsaAI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
Eco57MI EcoO109I FseI Hin4I KpnI MslI MspA1I NaeI
NcoI NgoMIV NspI PfoI PpuMI PstI PvuII SexAI
SfcI SmaI SmlI SphI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmnI ZraI