Restriction map for
pFN21AE9395
(Linear) (Six-base) MAPSORT of: KIEE9395.seq Check: 2318 from: 1 to: 1836
KIEE9395.orf
With 182 enzymes: SgfI *
January 29, 2014 12:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 258 1056 1836
Size: 258 798 780
Fragments arranged by size:
798 780 258
AclI AA'CG_TT
Cuts at: 0 279 1836
Size: 279 1557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1546 1836
Size: 1097 449 290
Fragments arranged by size:
1097 449 290
AflIII A'CryG_T
Cuts at: 0 859 1836
Size: 859 977
AleI CACnn'nnGTG
Cuts at: 0 260 1836
Size: 260 1576
ApoI r'AATT_y
Cuts at: 0 157 353 449 699 939 1018 1350 1836
Size: 157 196 96 250 240 79 332 486
Fragments arranged by size:
486 332 250 240 196 157 96 79
BanII G_rGCy'C
Cuts at: 0 48 1315 1836
Size: 48 1267 521
Fragments arranged by size:
1267 521 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 1138 1793 1836
Size: 14 1124 655 43
Fragments arranged by size:
1124 655 43 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 188 1292 1836
Size: 188 1104 544
Fragments arranged by size:
1104 544 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 554 1287 1836
Size: 446 108 733 549
Fragments arranged by size:
733 549 446 108
Bpu10I CC'TnA_GC
Cuts at: 0 1314 1836
Size: 1314 522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1467 1836
Size: 1467 369
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 527 557 1836
Size: 527 30 1279
Fragments arranged by size:
1279 527 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 1836
Size: 214 1622
BseYI C'CCAG_C
Cuts at: 0 13 324 1836
Size: 13 311 1512
Fragments arranged by size:
1512 311 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1019 1084 1836
Size: 709 310 65 752
Fragments arranged by size:
752 709 310 65
BsiEI CG_ry'CG
Cuts at: 0 33 1836
Size: 33 1803
BsmI GAATG_Cn'
Cuts at: 0 1706 1836
Size: 1706 130
Bsp1286I G_dGCh'C
Cuts at: 0 48 1315 1836
Size: 48 1267 521
Fragments arranged by size:
1267 521 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 61 258 1056 1656 1836
Size: 61 197 798 600 180
Fragments arranged by size:
798 600 197 180 61
BsrFI r'CCGG_y
Cuts at: 0 28 1836
Size: 28 1808
BstBI TT'CG_AA
Cuts at: 0 1608 1836
Size: 1608 228
BstYI r'GATC_y
Cuts at: 0 1242 1836
Size: 1242 594
BtsI GCAGTG_nn'
Cuts at: 0 654 1333 1836
Size: 654 679 503
Fragments arranged by size:
679 654 503
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 279 314 1836
Size: 279 35 1522
Fragments arranged by size:
1522 279 35
EaeI y'GGCC_r
Cuts at: 0 30 525 1161 1836
Size: 30 495 636 675
Fragments arranged by size:
675 636 495 30
EagI C'GGCC_G
Cuts at: 0 30 1836
Size: 30 1806
EarI CTCTTCn'nnn_
Cuts at: 0 209 476 1445 1565 1836
Size: 209 267 969 120 271
Fragments arranged by size:
969 271 267 209 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1836
Size: 19 1817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 554 1097 1287 1546 1836
Size: 446 108 543 190 259 290
Fragments arranged by size:
543 446 290 259 190 108
EcoRV GAT'ATC
Cuts at: 0 579 1836
Size: 579 1257
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 416 1110 1234 1836
Size: 416 694 124 602
Fragments arranged by size:
694 602 416 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 1836
Size: 1222 32 582
Fragments arranged by size:
1222 582 32
HincII GTy'rAC
Cuts at: 0 1622 1836
Size: 1622 214
HpaI GTT'AAC
Cuts at: 0 1622 1836
Size: 1622 214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1062 1836
Size: 1062 774
MscI TGG'CCA
Cuts at: 0 527 1163 1836
Size: 527 636 673
Fragments arranged by size:
673 636 527
MslI CAynn'nnrTG
Cuts at: 0 260 1836
Size: 260 1576
MspA1I CmG'CkG
Cuts at: 0 53 139 723 1823 1836
Size: 53 86 584 1100 13
Fragments arranged by size:
1100 584 86 53 13
NaeI GCC'GGC
Cuts at: 0 30 1836
Size: 30 1806
NgoMIV G'CCGG_C
Cuts at: 0 28 1836
Size: 28 1808
NspI r_CATG'y
Cuts at: 0 863 1836
Size: 863 973
PciI A'CATG_T
Cuts at: 0 859 1836
Size: 859 977
PflMI CCAn_nnn'nTGG
Cuts at: 0 1702 1836
Size: 1702 134
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 857 889 1836
Size: 857 32 947
Fragments arranged by size:
947 857 32
PvuII CAG'CTG
Cuts at: 0 139 723 1823 1836
Size: 139 584 1100 13
Fragments arranged by size:
1100 584 139 13
SapI GCTCTTCn'nnn_
Cuts at: 0 1445 1836
Size: 1445 391
ScaI AGT'ACT
Cuts at: 0 90 1836
Size: 90 1746
SfcI C'TryA_G
Cuts at: 0 258 1836
Size: 258 1578
SmlI C'TyrA_G
Cuts at: 0 1482 1836
Size: 1482 354
SspI AAT'ATT
Cuts at: 0 207 711 1635 1836
Size: 207 504 924 201
Fragments arranged by size:
924 504 207 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1408 1836
Size: 1408 428
TatI w'GTAC_w
Cuts at: 0 88 543 1836
Size: 88 455 1293
Fragments arranged by size:
1293 455 88
XbaI T'CTAG_A
Cuts at: 0 1749 1836
Size: 1749 87
Enzymes that do cut:
AarI AclI AcuI AflIII AleI ApoI BanII BbsI
BplI BpmI Bpu10I BpuEI BsaXI BseRI BseYI BsgI
BsiEI BsmI Bsp1286I BspMI BsrFI BstBI BstYI BtsI
CspCI EaeI EagI EarI EciI Eco57MI EcoRV FalI
Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PciI PflMI PsrI PvuII SapI ScaI
SfcI SmlI SspI TaqII TatI XbaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BsaI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
DraI DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI FseI
FspI FspAI HaeII HindIII KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI StyI SwaI TstI
Tth111I XcmI XhoI XmaI XmnI ZraI