Restriction map for
pFN21AE9394
(Linear) (Six-base) MAPSORT of: KIEE9394.seq Check: 2629 from: 1 to: 3489
KIEE9394.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AccI GT'mk_AC
Cuts at: 0 1769 2819 3489
Size: 1769 1050 670
Fragments arranged by size:
1769 1050 670
Acc65I G'GTAC_C
Cuts at: 0 2132 3489
Size: 2132 1357
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1454 1745 1765 3067 3085 3115 3227
Size: 880 574 291 20 1302 18 30 112
Cuts at: 3227 3489
Size: 262
Fragments arranged by size:
1302 880 574 291 262 112 30 20
18
AhdI GACnn_n'nnGTC
Cuts at: 0 459 2504 3489
Size: 459 2045 985
Fragments arranged by size:
2045 985 459
AleI CACnn'nnGTG
Cuts at: 0 455 3489
Size: 455 3034
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2429 3489
Size: 2429 1060
AlwNI CAG_nnn'CTG
Cuts at: 0 928 1723 3453 3489
Size: 928 795 1730 36
Fragments arranged by size:
1730 928 795 36
ApaI G_GGCC'C
Cuts at: 0 3397 3489
Size: 3397 92
ApoI r'AATT_y
Cuts at: 0 59 276 1030 2361 3099 3323 3364 3489
Size: 59 217 754 1331 738 224 41 125
Fragments arranged by size:
1331 754 738 224 217 125 59 41
AvaI C'yCGr_G
Cuts at: 0 638 1384 2005 3489
Size: 638 746 621 1484
Fragments arranged by size:
1484 746 638 621
BanI G'GyrC_C
Cuts at: 0 1118 2132 2961 3489
Size: 1118 1014 829 528
Fragments arranged by size:
1118 1014 829 528
BanII G_rGCy'C
Cuts at: 0 3397 3489
Size: 3397 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 80 1175 1847 2504 2602 2614 3489
Size: 17 63 1095 672 657 98 12 875
Fragments arranged by size:
1095 875 672 657 98 63 17 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2470 2504 3445 3479 3489
Size: 2470 34 941 34 10
Fragments arranged by size:
2470 941 34 34 10
BclI T'GATC_A
Cuts at: 0 600 3489
Size: 600 2889
BfrBI ATG'CAT
Cuts at: 0 919 2791 2863 3489
Size: 919 1872 72 626
Fragments arranged by size:
1872 919 626 72
BglII A'GATC_T
Cuts at: 0 205 3426 3489
Size: 205 3221 63
Fragments arranged by size:
3221 205 63
Bme1580I G_kGCm'C
Cuts at: 0 1121 1531 3397 3489
Size: 1121 410 1866 92
Fragments arranged by size:
1866 1121 410 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 666 1541 3489
Size: 666 875 1948
Fragments arranged by size:
1948 875 666
BmtI G_CTAG'C
Cuts at: 0 1781 3489
Size: 1781 1708
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1397 1994 2270 2502 3489
Size: 1397 597 276 232 987
Fragments arranged by size:
1397 987 597 276 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1097 1254 1776 3489
Size: 311 786 157 522 1713
Fragments arranged by size:
1713 786 522 311 157
Bpu10I CC'TnA_GC
Cuts at: 0 2879 3489
Size: 2879 610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1481 1806 3101 3489
Size: 1481 325 1295 388
Fragments arranged by size:
1481 1295 388 325
BsaI GGTCTCn'nnnn_
Cuts at: 0 593 3489
Size: 593 2896
BsaHI Gr'CG_yC
Cuts at: 0 77 3489
Size: 77 3412
BsaWI w'CCGG_w
Cuts at: 0 14 3489
Size: 14 3475
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1837 1867 2560 2590 3489
Size: 1837 30 693 30 899
Fragments arranged by size:
1837 899 693 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 86 1224 2025 2646 3489
Size: 86 1138 801 621 843
Fragments arranged by size:
1138 843 801 621 86
BseYI C'CCAG_C
Cuts at: 0 343 865 2446 3489
Size: 343 522 1581 1043
Fragments arranged by size:
1581 1043 522 343
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1634 2660 3439 3489
Size: 1634 1026 779 50
Fragments arranged by size:
1634 1026 779 50
BsiEI CG_ry'CG
Cuts at: 0 456 3489
Size: 456 3033
BsiHKAI G_wGCw'C
Cuts at: 0 821 3092 3489
Size: 821 2271 397
Fragments arranged by size:
2271 821 397
BsmI GAATG_Cn'
Cuts at: 0 972 2865 2880 3489
Size: 972 1893 15 609
Fragments arranged by size:
1893 972 609 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2542 2908 3489
Size: 2542 366 581
Fragments arranged by size:
2542 581 366
Bsp1286I G_dGCh'C
Cuts at: 0 821 1121 1531 3092 3397 3489
Size: 821 300 410 1561 305 92
Fragments arranged by size:
1561 821 410 305 300 92
BspHI T'CATG_A
Cuts at: 0 3419 3489
Size: 3419 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 403 3489
Size: 403 3086
BssSI C'ACGA_G
Cuts at: 0 1733 2244 3489
Size: 1733 511 1245
Fragments arranged by size:
1733 1245 511
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2968 3453 3489
Size: 2968 485 36
Fragments arranged by size:
2968 485 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 838 3489
Size: 838 2651
BstYI r'GATC_y
Cuts at: 0 205 1297 1315 2466 2922 3263 3426 3489
Size: 205 1092 18 1151 456 341 163 63
Fragments arranged by size:
1151 1092 456 341 205 163 63 18
BstZ17I GTA'TAC
Cuts at: 0 2820 3489
Size: 2820 669
Bsu36I CC'TnA_GG
Cuts at: 0 372 3031 3489
Size: 372 2659 458
Fragments arranged by size:
2659 458 372
BtgI C'CryG_G
Cuts at: 0 776 794 837 3489
Size: 776 18 43 2652
Fragments arranged by size:
2652 776 43 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3024 3110 3489
Size: 3024 86 379
Fragments arranged by size:
3024 379 86
BtsI GCAGTG_nn'
Cuts at: 0 758 1170 3489
Size: 758 412 2319
Fragments arranged by size:
2319 758 412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 704 739 3489
Size: 704 35 2750
Fragments arranged by size:
2750 704 35
DraIII CAC_nnn'GTG
Cuts at: 0 1657 3489
Size: 1657 1832
EarI CTCTTCn'nnn_
Cuts at: 0 1473 1721 2084 2488 3489
Size: 1473 248 363 404 1001
Fragments arranged by size:
1473 1001 404 363 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 63 3489
Size: 63 3426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 880 1097 1254 1454 1745 1765 1776
Size: 311 569 217 157 200 291 20 11
Cuts at: 1776 3067 3085 3115 3227 3489
Size: 1291 18 30 112 262
Fragments arranged by size:
1291 569 311 291 262 217 200 157
112 30 20 18 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1736 3489
Size: 1736 1753
EcoO109I rG'GnC_Cy
Cuts at: 0 334 642 3394 3489
Size: 334 308 2752 95
Fragments arranged by size:
2752 334 308 95
EcoRI G'AATT_C
Cuts at: 0 2361 3099 3364 3489
Size: 2361 738 265 125
Fragments arranged by size:
2361 738 265 125
EcoRV GAT'ATC
Cuts at: 0 3485 3489
Size: 3485 4
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1922 2736 2973 3257 3489
Size: 1922 814 237 284 232
Fragments arranged by size:
1922 814 284 237 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 250 282 445 477 1837 1869
Size: 127 32 91 32 163 32 1360 32
Cuts at: 1869 1930 1962 2490 2522 2779 2811 2907 2939
Size: 61 32 528 32 257 32 96 32
Cuts at: 2939 3489
Size: 550
Fragments arranged by size:
1360 550 528 257 163 127 96 91
61 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1895 3489
Size: 1895 1594
HindIII A'AGCT_T
Cuts at: 0 217 2713 3489
Size: 217 2496 776
Fragments arranged by size:
2496 776 217
KpnI G_GTAC'C
Cuts at: 0 2136 3489
Size: 2136 1353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 2306 3326 3489
Size: 1066 1240 1020 163
Fragments arranged by size:
1240 1066 1020 163
MslI CAynn'nnrTG
Cuts at: 0 455 596 1250 2306 3489
Size: 455 141 654 1056 1183
Fragments arranged by size:
1183 1056 654 455 141
MspA1I CmG'CkG
Cuts at: 0 402 1720 2446 2977 3489
Size: 402 1318 726 531 512
Fragments arranged by size:
1318 726 531 512 402
NcoI C'CATG_G
Cuts at: 0 776 794 837 3489
Size: 776 18 43 2652
Fragments arranged by size:
2652 776 43 18
NheI G'CTAG_C
Cuts at: 0 1777 3489
Size: 1777 1712
NsiI A_TGCA'T
Cuts at: 0 921 2793 2865 3489
Size: 921 1872 72 624
Fragments arranged by size:
1872 921 624 72
NspI r_CATG'y
Cuts at: 0 1788 3489
Size: 1788 1701
PflMI CCAn_nnn'nTGG
Cuts at: 0 912 3130 3273 3489
Size: 912 2218 143 216
Fragments arranged by size:
2218 912 216 143
PfoI T'CCnGG_A
Cuts at: 0 1584 3489
Size: 1584 1905
PpuMI rG'GwC_Cy
Cuts at: 0 334 642 3489
Size: 334 308 2847
Fragments arranged by size:
2847 334 308
PshAI GACnn'nnGTC
Cuts at: 0 789 3489
Size: 789 2700
PsiI TTA'TAA
Cuts at: 0 3107 3489
Size: 3107 382
PspOMI G'GGCC_C
Cuts at: 0 3393 3489
Size: 3393 96
PstI C_TGCA'G
Cuts at: 0 2982 3489
Size: 2982 507
PvuII CAG'CTG
Cuts at: 0 1720 2977 3489
Size: 1720 1257 512
Fragments arranged by size:
1720 1257 512
SanDI GG'GwC_CC
Cuts at: 0 642 3489
Size: 642 2847
SapI GCTCTTCn'nnn_
Cuts at: 0 1721 3489
Size: 1721 1768
ScaI AGT'ACT
Cuts at: 0 2999 3070 3489
Size: 2999 71 419
Fragments arranged by size:
2999 419 71
SfcI C'TryA_G
Cuts at: 0 239 822 1682 1766 1868 2972 2978 3489
Size: 239 583 860 84 102 1104 6 511
Fragments arranged by size:
1104 860 583 511 239 102 84 6
SmlI C'TyrA_G
Cuts at: 0 1496 1821 3116 3489
Size: 1496 325 1295 373
Fragments arranged by size:
1496 1295 373 325
SphI G_CATG'C
Cuts at: 0 1788 3489
Size: 1788 1701
StuI AGG'CCT
Cuts at: 0 1742 3489
Size: 1742 1747
StyI C'CwwG_G
Cuts at: 0 776 794 837 2210 2624 3489
Size: 776 18 43 1373 414 865
Fragments arranged by size:
1373 865 776 414 43 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 357 2614 3489
Size: 357 2257 875
Fragments arranged by size:
2257 875 357
TatI w'GTAC_w
Cuts at: 0 1213 2997 3068 3489
Size: 1213 1784 71 421
Fragments arranged by size:
1784 1213 421 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3248 3280 3489
Size: 3248 32 209
Fragments arranged by size:
3248 209 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1409 3489
Size: 1409 2080
XmnI GAAnn'nnTTC
Cuts at: 0 190 2736 3204 3489
Size: 190 2546 468 285
Fragments arranged by size:
2546 468 285 190
Enzymes that do cut:
AccI Acc65I AcuI AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BcgI BclI BfrBI
BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BssSI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraIII EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HincII HindIII KpnI MmeI MslI MspA1I
NcoI NheI NsiI NspI PflMI PfoI PpuMI PshAI
PsiI PspOMI PstI PvuII SanDI SapI ScaI SfcI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BglI BlpI BmgBI BsaAI BsaBI BsiWI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII
ClaI DraI DrdI EaeI EagI EcoICRI FseI FspI
FspAI HaeII HpaI KasI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NotI NruI PacI PasI PciI
PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI ZraI