(Linear) (Six-base) MAPSORT of: KIEE9294.seq  Check: 7667  from: 1  to: 897



KIEE9294.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     800     897 
   Size:        800      97
 
AlwNI CAG_nnn'CTG

Cuts at:      0     287     897 
   Size:        287     610
 
ApoI r'AATT_y

Cuts at:      0      60      90     897 
   Size:         60      30     807

  Fragments arranged by size: 

                 807      60      30
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     518     551     897 
   Size:        518      33     346

  Fragments arranged by size: 

                 518     346      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     771     897 
   Size:        771     126
 
BbvCI CC'TCA_GC

Cuts at:      0     304     897 
   Size:        304     593
 
BglII A'GATC_T

Cuts at:      0     119     897 
   Size:        119     778
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     650     897 
   Size:        650     247
 
BmtI G_CTAG'C

Cuts at:      0     547     897 
   Size:        547     350
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     897 
   Size:        144     753
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     897 
   Size:        304     593
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     601     632     897 
   Size:        307     294      31     265

  Fragments arranged by size: 

                 307     294     265      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     162     897 
   Size:        162     735
 
BsaAI yAC'GTr

Cuts at:      0     712     897 
   Size:        712     185
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     381     897 
   Size:        381     516
 
BsrDI GCAATG_nn'

Cuts at:      0     518     897 
   Size:        518     379
 
BsrFI r'CCGG_y

Cuts at:      0     271     897 
   Size:        271     626
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     799     897 
   Size:        799      98
 
BstYI r'GATC_y

Cuts at:      0     119     643     651     897 
   Size:        119     524       8     246

  Fragments arranged by size: 

                 524     246     119       8
 
BtgI C'CryG_G

Cuts at:      0     633     897 
   Size:        633     264
 
BtsI GCAGTG_nn'

Cuts at:      0     143     897 
   Size:        143     754
 
DraIII CAC_nnn'GTG

Cuts at:      0     101     897 
   Size:        101     796
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     139     897 
   Size:        139     758
 
EaeI y'GGCC_r

Cuts at:      0     273     897 
   Size:        273     624
 
EarI CTCTTCn'nnn_

Cuts at:      0     103     897 
   Size:        103     794
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     800     897 
   Size:        144     656      97

  Fragments arranged by size: 

                 656     144      97
 
EcoO109I rG'GnC_Cy

Cuts at:      0     403     897 
   Size:        403     494
 
EcoRV GAT'ATC

Cuts at:      0     578     897 
   Size:        578     319
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     141     897 
   Size:        141     756
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     897 
   Size:        104      32     761

  Fragments arranged by size: 

                 761     104      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     897 
   Size:        365     532
 
MslI CAynn'nnrTG

Cuts at:      0     388     897 
   Size:        388     509
 
MspA1I CmG'CkG

Cuts at:      0     809     897 
   Size:        809      88
 
NcoI C'CATG_G

Cuts at:      0     633     897 
   Size:        633     264
 
NheI G'CTAG_C

Cuts at:      0     543     897 
   Size:        543     354
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     101     897 
   Size:        101     796
 
PsiI TTA'TAA

Cuts at:      0     254     897 
   Size:        254     643
 
PvuII CAG'CTG

Cuts at:      0     809     897 
   Size:        809      88
 
SmlI C'TyrA_G

Cuts at:      0     322     616     647     897 
   Size:        322     294      31     250

  Fragments arranged by size: 

                 322     294     250      31
 
StyI C'CwwG_G

Cuts at:      0     633     662     897 
   Size:        633      29     235

  Fragments arranged by size: 

                 633     235      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      93     168     897 
   Size:         93      75     729

  Fragments arranged by size: 

                 729      93      75
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     897 
   Size:         36     861
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BaeI     BbsI    BbvCI    BglII     BmrI
     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BseRI    BsrDI
    BsrFI    BstXI    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI
     EarI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I     MmeI     MslI
   MspA1I     NcoI     NheI    PflMI     PsiI    PvuII     SmlI     StyI
    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BplI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI     EagI     EciI  EcoICRI
    EcoNI    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI