(Linear) (Six-base) MAPSORT of: KIEE9290.seq  Check: 4078  from: 1  to: 957



KIEE9290.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:29  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     287     957 
   Size:        287     670
 
ApoI r'AATT_y

Cuts at:      0      60      90     137     957 
   Size:         60      30      47     820

  Fragments arranged by size: 

                 820      60      47      30
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     771     945     957 
   Size:        771     174      12

  Fragments arranged by size: 

                 771     174      12
 
BbvCI CC'TCA_GC

Cuts at:      0     304     957 
   Size:        304     653
 
BclI T'GATC_A

Cuts at:      0     734     957 
   Size:        734     223
 
BglII A'GATC_T

Cuts at:      0     119     957 
   Size:        119     838
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     906     957 
   Size:        906      51
 
BmtI G_CTAG'C

Cuts at:      0     547     957 
   Size:        547     410
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     957 
   Size:        144     813
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     322     957 
   Size:        304      18     635

  Fragments arranged by size: 

                 635     304      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     632     957 
   Size:        601      31     325

  Fragments arranged by size: 

                 601     325      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     162     957 
   Size:        162     795
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     381     519     957 
   Size:        381     138     438

  Fragments arranged by size: 

                 438     381     138
 
BseYI C'CCAG_C

Cuts at:      0     655     957 
   Size:        655     302
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     925     957 
   Size:        925      32
 
BsrDI GCAATG_nn'

Cuts at:      0     518     957 
   Size:        518     439
 
BstYI r'GATC_y

Cuts at:      0     119     643     651     957 
   Size:        119     524       8     306

  Fragments arranged by size: 

                 524     306     119       8
 
BtgI C'CryG_G

Cuts at:      0     633     957 
   Size:        633     324
 
BtsI GCAGTG_nn'

Cuts at:      0     143     957 
   Size:        143     814
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     144     957 
   Size:        144     813
 
EcoO109I rG'GnC_Cy

Cuts at:      0     403     957 
   Size:        403     554
 
EcoRV GAT'ATC

Cuts at:      0     578     957 
   Size:        578     379
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     141     957 
   Size:        141     816
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     104     136     957 
   Size:        104      32     821

  Fragments arranged by size: 

                 821     104      32
 
HincII GTy'rAC

Cuts at:      0     132     957 
   Size:        132     825
 
HindIII A'AGCT_T

Cuts at:      0     677     957 
   Size:        677     280
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     957 
   Size:        365     592
 
MslI CAynn'nnrTG

Cuts at:      0     197     957 
   Size:        197     760
 
MspA1I CmG'CkG

Cuts at:      0     287     957 
   Size:        287     670
 
NcoI C'CATG_G

Cuts at:      0     633     957 
   Size:        633     324
 
NheI G'CTAG_C

Cuts at:      0     543     957 
   Size:        543     414
 
NspI r_CATG'y

Cuts at:      0     312     889     957 
   Size:        312     577      68

  Fragments arranged by size: 

                 577     312      68
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     101     957 
   Size:        101     856
 
PsiI TTA'TAA

Cuts at:      0     254     957 
   Size:        254     703
 
PvuII CAG'CTG

Cuts at:      0     287     957 
   Size:        287     670
 
SmlI C'TyrA_G

Cuts at:      0     616     647     957 
   Size:        616      31     310

  Fragments arranged by size: 

                 616     310      31
 
StyI C'CwwG_G

Cuts at:      0     633     662     957 
   Size:        633      29     295

  Fragments arranged by size: 

                 633     295      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     168     839     888     957 
   Size:        168     671      49      69

  Fragments arranged by size: 

                 671     168      69      49
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     957 
   Size:         36     921
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI    BbvCI     BclI    BglII     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BseYI    BsmBI    BsrDI
    BstYI     BtgI     BtsI  Eco57MI EcoO109I    EcoRV     FalI    Hin4I
   HincII  HindIII     MmeI     MslI   MspA1I     NcoI     NheI     NspI
    PflMI     PsiI    PvuII     SmlI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI