Restriction map for
pFN21AE9289
(Linear) (Six-base) MAPSORT of: KIEE9289.seq Check: 1797 from: 1 to: 912
KIEE9289.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 209 912
Size: 209 703
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 912
Size: 735 177
AflIII A'CryG_T
Cuts at: 0 377 912
Size: 377 535
AleI CACnn'nnGTG
Cuts at: 0 154 912
Size: 154 758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 442 912
Size: 442 470
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 298 330 912
Size: 298 32 582
Fragments arranged by size:
582 298 32
AlwNI CAG_nnn'CTG
Cuts at: 0 67 912
Size: 67 845
BanII G_rGCy'C
Cuts at: 0 89 107 191 599 912
Size: 89 18 84 408 313
Fragments arranged by size:
408 313 89 84 18
BclI T'GATC_A
Cuts at: 0 173 912
Size: 173 739
BfrBI ATG'CAT
Cuts at: 0 329 912
Size: 329 583
Bme1580I G_kGCm'C
Cuts at: 0 887 912
Size: 887 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 591 912
Size: 91 500 321
Fragments arranged by size:
500 321 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 663 912
Size: 186 477 249
Fragments arranged by size:
477 249 186
BsaHI Gr'CG_yC
Cuts at: 0 664 912
Size: 664 248
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 298 328 912
Size: 298 30 584
Fragments arranged by size:
584 298 30
BseYI C'CCAG_C
Cuts at: 0 82 912
Size: 82 830
BsiHKAI G_wGCw'C
Cuts at: 0 107 912
Size: 107 805
BsmI GAATG_Cn'
Cuts at: 0 331 719 805 912
Size: 331 388 86 107
Fragments arranged by size:
388 331 107 86
Bsp1286I G_dGCh'C
Cuts at: 0 89 107 191 599 887 912
Size: 89 18 84 408 288 25
Fragments arranged by size:
408 288 89 84 25 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 728 824 912
Size: 209 519 96 88
Fragments arranged by size:
519 209 96 88
BsrDI GCAATG_nn'
Cuts at: 0 701 912
Size: 701 211
BsrFI r'CCGG_y
Cuts at: 0 517 912
Size: 517 395
BsrGI T'GTAC_A
Cuts at: 0 431 912
Size: 431 481
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 912
Size: 282 630
BstYI r'GATC_y
Cuts at: 0 502 912
Size: 502 410
BtgI C'CryG_G
Cuts at: 0 839 912
Size: 839 73
BtsI GCAGTG_nn'
Cuts at: 0 456 912
Size: 456 456
EarI CTCTTCn'nnn_
Cuts at: 0 124 586 912
Size: 124 462 326
Fragments arranged by size:
462 326 124
EcoICRI GAG'CTC
Cuts at: 0 105 912
Size: 105 807
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 591 735 912
Size: 91 500 144 177
Fragments arranged by size:
500 177 144 91
EcoRV GAT'ATC
Cuts at: 0 249 912
Size: 249 663
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 724 756 912
Size: 724 32 156
Fragments arranged by size:
724 156 32
MslI CAynn'nnrTG
Cuts at: 0 154 912
Size: 154 758
MspA1I CmG'CkG
Cuts at: 0 111 912
Size: 111 801
NcoI C'CATG_G
Cuts at: 0 839 912
Size: 839 73
NdeI CA'TA_TG
Cuts at: 0 396 912
Size: 396 516
NsiI A_TGCA'T
Cuts at: 0 331 912
Size: 331 581
NspI r_CATG'y
Cuts at: 0 381 912
Size: 381 531
PciI A'CATG_T
Cuts at: 0 377 912
Size: 377 535
PflMI CCAn_nnn'nTGG
Cuts at: 0 774 912
Size: 774 138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 912
Size: 298 32 582
Fragments arranged by size:
582 298 32
PstI C_TGCA'G
Cuts at: 0 421 804 821 912
Size: 421 383 17 91
Fragments arranged by size:
421 383 91 17
PvuII CAG'CTG
Cuts at: 0 111 912
Size: 111 801
SacI G_AGCT'C
Cuts at: 0 107 912
Size: 107 805
SfcI C'TryA_G
Cuts at: 0 417 800 817 905 912
Size: 417 383 17 88 7
Fragments arranged by size:
417 383 88 17 7
SmlI C'TyrA_G
Cuts at: 0 165 642 912
Size: 165 477 270
Fragments arranged by size:
477 270 165
StyI C'CwwG_G
Cuts at: 0 498 839 912
Size: 498 341 73
Fragments arranged by size:
498 341 73
TatI w'GTAC_w
Cuts at: 0 215 431 912
Size: 215 216 481
Fragments arranged by size:
481 216 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 912
Size: 128 32 752
Fragments arranged by size:
752 128 32
XmnI GAAnn'nnTTC
Cuts at: 0 54 912
Size: 54 858
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AloI AlwNI BanII
BclI BfrBI Bme1580I BpmI BpuEI BsaHI BsaXI BseYI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI
BstYI BtgI BtsI EarI EcoICRI Eco57MI EcoRV Hin4I
MslI MspA1I NcoI NdeI NsiI NspI PciI PflMI
PpiI PstI PvuII SacI SfcI SmlI StyI TatI
TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
XmaI ZraI