(Linear) (Six-base) MAPSORT of: KIEE9286.seq  Check: 4273  from: 1  to: 804



KIEE9286.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:29  ..

AvaI C'yCGr_G

Cuts at:      0     683     804 
   Size:        683     121
 
BamHI G'GATC_C

Cuts at:      0     294     804 
   Size:        294     510
 
BanII G_rGCy'C

Cuts at:      0     153     399     492     804 
   Size:        153     246      93     312

  Fragments arranged by size: 

                 312     246     153      93
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     526     804 
   Size:        526     278
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     139     804 
   Size:        139     665
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     545     804 
   Size:        383     162     259

  Fragments arranged by size: 

                 383     259     162
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     604     804 
   Size:        604     200
 
BsiHKAI G_wGCw'C

Cuts at:      0     153     399     492     804 
   Size:        153     246      93     312

  Fragments arranged by size: 

                 312     246     153      93
 
Bsp1286I G_dGCh'C

Cuts at:      0     153     399     492     804 
   Size:        153     246      93     312

  Fragments arranged by size: 

                 312     246     153      93
 
BsrDI GCAATG_nn'

Cuts at:      0     434     804 
   Size:        434     370
 
BstYI r'GATC_y

Cuts at:      0     294     804 
   Size:        294     510
 
EaeI y'GGCC_r

Cuts at:      0     663     804 
   Size:        663     141
 
EarI CTCTTCn'nnn_

Cuts at:      0     108     527     804 
   Size:        108     419     277

  Fragments arranged by size: 

                 419     277     108
 
EcoICRI GAG'CTC

Cuts at:      0     151     397     490     804 
   Size:        151     246      93     314

  Fragments arranged by size: 

                 314     246     151      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     383     545     804 
   Size:        383     162     259

  Fragments arranged by size: 

                 383     259     162
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     193     225     479     511     804 
   Size:        193      32     254      32     293

  Fragments arranged by size: 

                 293     254     193      32      32
 
HincII GTy'rAC

Cuts at:      0     283     540     804 
   Size:        283     257     264

  Fragments arranged by size: 

                 283     264     257
 
MscI TGG'CCA

Cuts at:      0     665     804 
   Size:        665     139
 
MslI CAynn'nnrTG

Cuts at:      0     173     200     479     556     649     804 
   Size:        173      27     279      77      93     155

  Fragments arranged by size: 

                 279     173     155      93      77      27
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     222     804 
   Size:        222     582
 
PfoI T'CCnGG_A

Cuts at:      0     399     804 
   Size:        399     405
 
SacI G_AGCT'C

Cuts at:      0     153     399     492     804 
   Size:        153     246      93     312

  Fragments arranged by size: 

                 312     246     153      93
 
StuI AGG'CCT

Cuts at:      0     376     804 
   Size:        376     428
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     268     500     804 
   Size:        268     232     304

  Fragments arranged by size: 

                 304     268     232
 
TatI w'GTAC_w

Cuts at:      0     614     804 
   Size:        614     190
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     673     804 
   Size:        673     131
 
XmnI GAAnn'nnTTC

Cuts at:      0      96     804 
   Size:         96     708
 

 Enzymes that do cut:

     AvaI    BamHI    BanII     BbsI     BplI     BpmI    BseRI  BsiHKAI
 Bsp1286I    BsrDI    BstYI     EaeI     EarI  EcoICRI  Eco57MI    Hin4I
   HincII     MscI     MslI    PflMI     PfoI     SacI     StuI    TaqII
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI