Restriction map for
pFN21AE9284
(Linear) (Six-base) MAPSORT of: KIEE9284.seq Check: 2130 from: 1 to: 981
KIEE9284.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 189 411 981
Size: 189 222 570
Fragments arranged by size:
570 222 189
AccI GT'mk_AC
Cuts at: 0 373 599 981
Size: 373 226 382
Fragments arranged by size:
382 373 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 755 981
Size: 475 280 226
Fragments arranged by size:
475 280 226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 979 981
Size: 979 2
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 981
Size: 316 32 633
Fragments arranged by size:
633 316 32
BamHI G'GATC_C
Cuts at: 0 164 981
Size: 164 817
BanI G'GyrC_C
Cuts at: 0 877 981
Size: 877 104
BanII G_rGCy'C
Cuts at: 0 213 445 863 974 981
Size: 213 232 418 111 7
Fragments arranged by size:
418 232 213 111 7
BclI T'GATC_A
Cuts at: 0 840 981
Size: 840 141
BglII A'GATC_T
Cuts at: 0 483 981
Size: 483 498
BlpI GC'TnA_GC
Cuts at: 0 551 808 981
Size: 551 257 173
Fragments arranged by size:
551 257 173
BmgBI CAC'GTC
Cuts at: 0 411 855 981
Size: 411 444 126
Fragments arranged by size:
444 411 126
BmrI ACTGGGnnnn_n'
Cuts at: 0 116 981
Size: 116 865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 981
Size: 376 605
BsaAI yAC'GTr
Cuts at: 0 606 981
Size: 606 375
BsaWI w'CCGG_w
Cuts at: 0 40 981
Size: 40 941
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 343 346 373 981
Size: 316 27 3 27 608
Fragments arranged by size:
608 316 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 981
Size: 340 641
BseYI C'CCAG_C
Cuts at: 0 82 981
Size: 82 899
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 676 981
Size: 217 459 305
Fragments arranged by size:
459 305 217
BsiEI CG_ry'CG
Cuts at: 0 942 981
Size: 942 39
BsiHKAI G_wGCw'C
Cuts at: 0 809 981
Size: 809 172
BsmI GAATG_Cn'
Cuts at: 0 968 981
Size: 968 13
Bsp1286I G_dGCh'C
Cuts at: 0 213 445 809 863 974 981
Size: 213 232 364 54 111 7
Fragments arranged by size:
364 232 213 111 54 7
BspHI T'CATG_A
Cuts at: 0 759 981
Size: 759 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 411 981
Size: 189 222 570
Fragments arranged by size:
570 222 189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 981
Size: 90 891
BstYI r'GATC_y
Cuts at: 0 164 483 764 981
Size: 164 319 281 217
Fragments arranged by size:
319 281 217 164
BstZ17I GTA'TAC
Cuts at: 0 374 981
Size: 374 607
BtgI C'CryG_G
Cuts at: 0 278 981
Size: 278 703
BtsI GCAGTG_nn'
Cuts at: 0 636 981
Size: 636 345
DraIII CAC_nnn'GTG
Cuts at: 0 279 981
Size: 279 702
DrdI GACnn_nn'nnGTC
Cuts at: 0 409 981
Size: 409 572
EaeI y'GGCC_r
Cuts at: 0 569 981
Size: 569 412
EarI CTCTTCn'nnn_
Cuts at: 0 278 981
Size: 278 703
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 475 755 981
Size: 376 99 280 226
Fragments arranged by size:
376 280 226 99
EcoNI CCTnn'n_nnAGG
Cuts at: 0 772 981
Size: 772 209
EcoO109I rG'GnC_Cy
Cuts at: 0 747 981
Size: 747 234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 729 981
Size: 729 252
HaeII r_GCGC'y
Cuts at: 0 680 981
Size: 680 301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 195 227 253 285 549 581 935 967
Size: 195 32 26 32 264 32 354 32
Cuts at: 967 981
Size: 14
Fragments arranged by size:
354 264 195 32 32 32 32 26
14
MscI TGG'CCA
Cuts at: 0 571 981
Size: 571 410
MslI CAynn'nnrTG
Cuts at: 0 685 981
Size: 685 296
MspA1I CmG'CkG
Cuts at: 0 53 981
Size: 53 928
NspI r_CATG'y
Cuts at: 0 174 981
Size: 174 807
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 981
Size: 279 702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 981
Size: 316 32 633
Fragments arranged by size:
633 316 32
PpuMI rG'GwC_Cy
Cuts at: 0 747 981
Size: 747 234
PstI C_TGCA'G
Cuts at: 0 27 981
Size: 27 954
SfcI C'TryA_G
Cuts at: 0 23 981
Size: 23 958
SphI G_CATG'C
Cuts at: 0 174 981
Size: 174 807
SspI AAT'ATT
Cuts at: 0 310 981
Size: 310 671
StuI AGG'CCT
Cuts at: 0 730 778 981
Size: 730 48 203
Fragments arranged by size:
730 203 48
StyI C'CwwG_G
Cuts at: 0 320 981
Size: 320 661
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 956 981
Size: 956 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 471 503 981
Size: 471 32 478
Fragments arranged by size:
478 471 32
XmnI GAAnn'nnTTC
Cuts at: 0 922 981
Size: 922 59
Enzymes that do cut:
AarI AccI AcuI AlfI AloI BamHI BanI BanII
BclI BglII BlpI BmgBI BmrI BpmI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BstXI BstYI BstZ17I BtgI BtsI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I MscI MslI MspA1I NspI PflMI PpiI PpuMI
PstI SfcI SphI SspI StuI StyI TaqII TstI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI Bme1580I BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI
ClaI CspCI DraI EagI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI ZraI