Restriction map for
pFN21AE9283
(Linear) (Six-base) MAPSORT of: KIEE9283.seq Check: 2534 from: 1 to: 576
KIEE9283.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 528 576
Size: 528 48
ApoI r'AATT_y
Cuts at: 0 556 576
Size: 556 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 22 55 576
Size: 22 33 521
Fragments arranged by size:
521 33 22
BanI G'GyrC_C
Cuts at: 0 16 576
Size: 16 560
BbeI G_GCGC'C
Cuts at: 0 20 576
Size: 20 556
BglI GCCn_nnn'nGGC
Cuts at: 0 309 367 576
Size: 309 58 209
Fragments arranged by size:
309 209 58
BmrI ACTGGGnnnn_n'
Cuts at: 0 504 576
Size: 504 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 312 576
Size: 312 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 552 576
Size: 550 2 24
Fragments arranged by size:
550 24 2
Bpu10I CC'TnA_GC
Cuts at: 0 155 576
Size: 155 421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 576
Size: 124 452
BsaHI Gr'CG_yC
Cuts at: 0 17 576
Size: 17 559
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 523 553 576
Size: 92 30 401 30 23
Fragments arranged by size:
401 92 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 547 576
Size: 256 291 29
Fragments arranged by size:
291 256 29
BseYI C'CCAG_C
Cuts at: 0 9 233 299 425 576
Size: 9 224 66 126 151
Fragments arranged by size:
224 151 126 66 9
BsrBI CCG'CTC
Cuts at: 0 566 576
Size: 566 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 576
Size: 312 264
BtgI C'CryG_G
Cuts at: 0 22 510 576
Size: 22 488 66
Fragments arranged by size:
488 66 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 552 576
Size: 550 2 24
Fragments arranged by size:
550 24 2
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 576
Size: 228 348
HaeII r_GCGC'y
Cuts at: 0 20 85 528 576
Size: 20 65 443 48
Fragments arranged by size:
443 65 48 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 442 474 576
Size: 92 32 318 32 102
Fragments arranged by size:
318 102 92 32 32
KasI G'GCGC_C
Cuts at: 0 16 576
Size: 16 560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 521 576
Size: 380 141 55
Fragments arranged by size:
380 141 55
MspA1I CmG'CkG
Cuts at: 0 9 24 44 233 420 576
Size: 9 15 20 189 187 156
Fragments arranged by size:
189 187 156 20 15 9
NarI GG'CG_CC
Cuts at: 0 17 576
Size: 17 559
NcoI C'CATG_G
Cuts at: 0 510 576
Size: 510 66
PfoI T'CCnGG_A
Cuts at: 0 58 185 576
Size: 58 127 391
Fragments arranged by size:
391 127 58
PstI C_TGCA'G
Cuts at: 0 89 576
Size: 89 487
RsrII CG'GwC_CG
Cuts at: 0 384 576
Size: 384 192
SacII CC_GC'GG
Cuts at: 0 25 576
Size: 25 551
SfcI C'TryA_G
Cuts at: 0 85 576
Size: 85 491
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 367 576
Size: 367 209
SfoI GGC'GCC
Cuts at: 0 18 576
Size: 18 558
SmlI C'TyrA_G
Cuts at: 0 139 576
Size: 139 437
StuI AGG'CCT
Cuts at: 0 211 361 576
Size: 211 150 215
Fragments arranged by size:
215 211 150
StyI C'CwwG_G
Cuts at: 0 510 576
Size: 510 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 91 417 576
Size: 91 326 159
Fragments arranged by size:
326 159 91
Tth111I GACn'n_nGTC
Cuts at: 0 496 576
Size: 496 80
XmnI GAAnn'nnTTC
Cuts at: 0 67 576
Size: 67 509
Enzymes that do cut:
AlwNI ApoI BaeI BanI BbeI BglI BmrI BplI
BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsrBI
BstXI BtgI Eco57MI FalI HaeII Hin4I KasI MmeI
MspA1I NarI NcoI PfoI PstI RsrII SacII SfcI
SfiI SfoI SmlI StuI StyI TaqII Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BamHI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmtI BsaI BsaAI BsaBI BsaWI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI XbaI
XcmI XhoI XmaI ZraI