(Linear) (Six-base) MAPSORT of: KIEE9277.seq  Check: 8516  from: 1  to: 612



KIEE9277.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:29  ..

AccI GT'mk_AC

Cuts at:      0     458     612 
   Size:        458     154
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     566     612 
   Size:        112     454      46

  Fragments arranged by size: 

                 454     112      46
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     412     612 
   Size:        412     200
 
AlwNI CAG_nnn'CTG

Cuts at:      0      28     612 
   Size:         28     584
 
ApoI r'AATT_y

Cuts at:      0      49     612 
   Size:         49     563
 
AvaI C'yCGr_G

Cuts at:      0     532     612 
   Size:        532      80
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     247     612 
   Size:        247     365
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     451     612 
   Size:        451     161
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     535     612 
   Size:        535      77
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     293     612 
   Size:        293     319
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     244     612 
   Size:         14     230     368

  Fragments arranged by size: 

                 368     230      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     437     612 
   Size:        437     175
 
Bsp1286I G_dGCh'C

Cuts at:      0     437     612 
   Size:        437     175
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     278     370     444     612 
   Size:        278      92      74     168

  Fragments arranged by size: 

                 278     168      92      74
 
BsrBI CCG'CTC

Cuts at:      0     245     612 
   Size:        245     367
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     321     510     595     612 
   Size:        321     189      85      17

  Fragments arranged by size: 

                 321     189      85      17
 
BstYI r'GATC_y

Cuts at:      0     488     612 
   Size:        488     124
 
BtgI C'CryG_G

Cuts at:      0     539     612 
   Size:        539      73
 
BtsI GCAGTG_nn'

Cuts at:      0     152     612 
   Size:        152     460
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     196     612 
   Size:        196     416
 
EaeI y'GGCC_r

Cuts at:      0     332     410     500     612 
   Size:        332      78      90     112

  Fragments arranged by size: 

                 332     112      90      78
 
EarI CTCTTCn'nnn_

Cuts at:      0     107     232     406     589     612 
   Size:        107     125     174     183      23

  Fragments arranged by size: 

                 183     174     125     107      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     566     612 
   Size:        112     454      46

  Fragments arranged by size: 

                 454     112      46
 
HindIII A'AGCT_T

Cuts at:      0     115     612 
   Size:        115     497
 
MscI TGG'CCA

Cuts at:      0     334     502     612 
   Size:        334     168     110

  Fragments arranged by size: 

                 334     168     110
 
MslI CAynn'nnrTG

Cuts at:      0     424     612 
   Size:        424     188
 
MspA1I CmG'CkG

Cuts at:      0      25     612 
   Size:         25     587
 
NcoI C'CATG_G

Cuts at:      0     539     612 
   Size:        539      73
 
PvuII CAG'CTG

Cuts at:      0      25     612 
   Size:         25     587
 
SapI GCTCTTCn'nnn_

Cuts at:      0     232     406     612 
   Size:        232     174     206

  Fragments arranged by size: 

                 232     206     174
 
SfcI C'TryA_G

Cuts at:      0     177     207     612 
   Size:        177      30     405

  Fragments arranged by size: 

                 405     177      30
 
SmlI C'TyrA_G

Cuts at:      0     466     612 
   Size:        466     146
 
StuI AGG'CCT

Cuts at:      0      13     472     612 
   Size:         13     459     140

  Fragments arranged by size: 

                 459     140      13
 
StyI C'CwwG_G

Cuts at:      0     539     612 
   Size:        539      73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55     612 
   Size:         55     557
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     546     612 
   Size:        546      66
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI     AvaI     BmrI    BpuEI
     BsaI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BstXI
    BstYI     BtgI     BtsI     DrdI     EaeI     EarI  Eco57MI  HindIII
     MscI     MslI   MspA1I     NcoI    PvuII     SapI     SfcI     SmlI
     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI