Restriction map for
pFN21AE9275
(Linear) (Six-base) MAPSORT of: KIEE9275.seq Check: 9223 from: 1 to: 804
KIEE9275.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AatII G_ACGT'C
Cuts at: 0 400 804
Size: 400 404
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 569 804
Size: 557 12 235
Fragments arranged by size:
557 235 12
AlwNI CAG_nnn'CTG
Cuts at: 0 592 804
Size: 592 212
ApoI r'AATT_y
Cuts at: 0 286 804
Size: 286 518
AseI AT'TA_AT
Cuts at: 0 269 804
Size: 269 535
AvaI C'yCGr_G
Cuts at: 0 52 804
Size: 52 752
BamHI G'GATC_C
Cuts at: 0 199 804
Size: 199 605
BanII G_rGCy'C
Cuts at: 0 441 601 804
Size: 441 160 203
Fragments arranged by size:
441 203 160
BbvCI CC'TCA_GC
Cuts at: 0 338 529 804
Size: 338 191 275
Fragments arranged by size:
338 275 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 398 804
Size: 364 34 406
Fragments arranged by size:
406 364 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 609 804
Size: 437 172 195
Fragments arranged by size:
437 195 172
Bpu10I CC'TnA_GC
Cuts at: 0 338 529 804
Size: 338 191 275
Fragments arranged by size:
338 275 191
BsaHI Gr'CG_yC
Cuts at: 0 397 804
Size: 397 407
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 155 185 372 402 435 465 607 637
Size: 155 30 187 30 33 30 142 30
Cuts at: 637 804
Size: 167
Fragments arranged by size:
187 167 155 142 33 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 650 804
Size: 650 154
BseYI C'CCAG_C
Cuts at: 0 651 804
Size: 651 153
BsiEI CG_ry'CG
Cuts at: 0 76 804
Size: 76 728
BsiHKAI G_wGCw'C
Cuts at: 0 441 601 804
Size: 441 160 203
Fragments arranged by size:
441 203 160
BsmI GAATG_Cn'
Cuts at: 0 314 804
Size: 314 490
Bsp1286I G_dGCh'C
Cuts at: 0 441 601 804
Size: 441 160 203
Fragments arranged by size:
441 203 160
BspHI T'CATG_A
Cuts at: 0 273 746 804
Size: 273 473 58
Fragments arranged by size:
473 273 58
BsrDI GCAATG_nn'
Cuts at: 0 39 804
Size: 39 765
BsrFI r'CCGG_y
Cuts at: 0 722 804
Size: 722 82
BstYI r'GATC_y
Cuts at: 0 199 804
Size: 199 605
Bsu36I CC'TnA_GG
Cuts at: 0 46 804
Size: 46 758
BtgI C'CryG_G
Cuts at: 0 76 804
Size: 76 728
BtsI GCAGTG_nn'
Cuts at: 0 576 636 795 804
Size: 576 60 159 9
Fragments arranged by size:
576 159 60 9
DraI TTT'AAA
Cuts at: 0 111 804
Size: 111 693
EaeI y'GGCC_r
Cuts at: 0 73 724 804
Size: 73 651 80
Fragments arranged by size:
651 80 73
EagI C'GGCC_G
Cuts at: 0 73 804
Size: 73 731
EarI CTCTTCn'nnn_
Cuts at: 0 588 606 804
Size: 588 18 198
Fragments arranged by size:
588 198 18
EcoICRI GAG'CTC
Cuts at: 0 439 599 804
Size: 439 160 205
Fragments arranged by size:
439 205 160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 557 569 609 804
Size: 437 120 12 40 195
Fragments arranged by size:
437 195 120 40 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 163 804
Size: 163 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 155 187 475 507 607 639 804
Size: 155 32 288 32 100 32 165
Fragments arranged by size:
288 165 155 100 32 32 32
HincII GTy'rAC
Cuts at: 0 543 804
Size: 543 261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 466 488 804
Size: 438 28 22 316
Fragments arranged by size:
438 316 28 22
MslI CAynn'nnrTG
Cuts at: 0 217 319 804
Size: 217 102 485
Fragments arranged by size:
485 217 102
MspA1I CmG'CkG
Cuts at: 0 21 78 804
Size: 21 57 726
Fragments arranged by size:
726 57 21
NaeI GCC'GGC
Cuts at: 0 724 804
Size: 724 80
NgoMIV G'CCGG_C
Cuts at: 0 722 804
Size: 722 82
NotI GC'GGCC_GC
Cuts at: 0 73 804
Size: 73 731
SacI G_AGCT'C
Cuts at: 0 441 601 804
Size: 441 160 203
Fragments arranged by size:
441 203 160
SacII CC_GC'GG
Cuts at: 0 79 804
Size: 79 725
SapI GCTCTTCn'nnn_
Cuts at: 0 588 606 804
Size: 588 18 198
Fragments arranged by size:
588 198 18
SfcI C'TryA_G
Cuts at: 0 122 804
Size: 122 682
StuI AGG'CCT
Cuts at: 0 699 804
Size: 699 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 804
Size: 225 579
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 804
Size: 372 32 400
Fragments arranged by size:
400 372 32
ZraI GAC'GTC
Cuts at: 0 398 804
Size: 398 406
Enzymes that do cut:
AatII AcuI AlwNI ApoI AseI AvaI BamHI BanII
BbvCI BcgI BpmI Bpu10I BsaHI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BstYI
Bsu36I BtgI BtsI DraI EaeI EagI EarI EcoICRI
Eco57MI FalI Hin4I HincII MmeI MslI MspA1I NaeI
NgoMIV NotI SacI SacII SapI SfcI StuI TaqII
TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BanI BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiWI BsmBI
BspEI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI
EciI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MscI
NarI NcoI NdeI NheI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StyI SwaI TatI Tth111I XbaI XcmI XhoI
XmaI XmnI