Restriction map for
pFN21AE9258
(Linear) (Six-base) MAPSORT of: KIEE9258.seq Check: 943 from: 1 to: 858
KIEE9258.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AccI GT'mk_AC
Cuts at: 0 425 622 858
Size: 425 197 236
Fragments arranged by size:
425 236 197
AflIII A'CryG_T
Cuts at: 0 392 858
Size: 392 466
AgeI A'CCGG_T
Cuts at: 0 152 858
Size: 152 706
ApoI r'AATT_y
Cuts at: 0 557 858
Size: 557 301
BanI G'GyrC_C
Cuts at: 0 372 858
Size: 372 486
BbeI G_GCGC'C
Cuts at: 0 376 858
Size: 376 482
BbsI GAAGACnn'nnnn_
Cuts at: 0 697 858
Size: 697 161
BclI T'GATC_A
Cuts at: 0 713 858
Size: 713 145
BfrBI ATG'CAT
Cuts at: 0 777 858
Size: 777 81
BglI GCCn_nnn'nGGC
Cuts at: 0 59 858
Size: 59 799
BmrI ACTGGGnnnn_n'
Cuts at: 0 498 669 858
Size: 498 171 189
Fragments arranged by size:
498 189 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 429 760 858
Size: 362 67 331 98
Fragments arranged by size:
362 331 98 67
BsaHI Gr'CG_yC
Cuts at: 0 373 858
Size: 373 485
BsaWI w'CCGG_w
Cuts at: 0 152 858
Size: 152 706
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 401 431 858
Size: 401 30 427
Fragments arranged by size:
427 401 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 108 858
Size: 108 750
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 719 858
Size: 156 563 139
Fragments arranged by size:
563 156 139
BsiHKAI G_wGCw'C
Cuts at: 0 144 858
Size: 144 714
Bsp1286I G_dGCh'C
Cuts at: 0 144 858
Size: 144 714
BsrBI CCG'CTC
Cuts at: 0 553 858
Size: 553 305
BsrDI GCAATG_nn'
Cuts at: 0 813 858
Size: 813 45
BsrFI r'CCGG_y
Cuts at: 0 152 369 858
Size: 152 217 489
Fragments arranged by size:
489 217 152
BstZ17I GTA'TAC
Cuts at: 0 623 858
Size: 623 235
BtgI C'CryG_G
Cuts at: 0 57 858
Size: 57 801
DraI TTT'AAA
Cuts at: 0 509 858
Size: 509 349
DrdI GACnn_nn'nnGTC
Cuts at: 0 418 858
Size: 418 440
EaeI y'GGCC_r
Cuts at: 0 38 858
Size: 38 820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 429 760 858
Size: 362 67 331 98
Fragments arranged by size:
362 331 98 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 210 858
Size: 210 648
HaeII r_GCGC'y
Cuts at: 0 376 858
Size: 376 482
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 401 433 461 493 858
Size: 401 32 28 32 365
Fragments arranged by size:
401 365 32 32 28
KasI G'GCGC_C
Cuts at: 0 372 858
Size: 372 486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 858
Size: 652 206
MspA1I CmG'CkG
Cuts at: 0 59 858
Size: 59 799
NaeI GCC'GGC
Cuts at: 0 371 858
Size: 371 487
NarI GG'CG_CC
Cuts at: 0 373 858
Size: 373 485
NgoMIV G'CCGG_C
Cuts at: 0 369 858
Size: 369 489
NsiI A_TGCA'T
Cuts at: 0 779 858
Size: 779 79
NspI r_CATG'y
Cuts at: 0 781 858
Size: 781 77
PasI CC'CwG_GG
Cuts at: 0 753 858
Size: 753 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 310 759 858
Size: 310 449 99
Fragments arranged by size:
449 310 99
PshAI GACnn'nnGTC
Cuts at: 0 112 858
Size: 112 746
SacII CC_GC'GG
Cuts at: 0 60 858
Size: 60 798
SfoI GGC'GCC
Cuts at: 0 374 858
Size: 374 484
StuI AGG'CCT
Cuts at: 0 199 858
Size: 199 659
StyI C'CwwG_G
Cuts at: 0 35 858
Size: 35 823
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 674 858
Size: 674 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 681 713 754 786 858
Size: 681 32 41 32 72
Fragments arranged by size:
681 72 41 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 415 858
Size: 415 443
Enzymes that do cut:
AccI AflIII AgeI ApoI BanI BbeI BbsI BclI
BfrBI BglI BmrI BpmI BsaHI BsaWI BsaXI BseRI
BsgI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstZ17I BtgI
DraI DrdI EaeI Eco57MI EcoNI HaeII Hin4I KasI
MmeI MspA1I NaeI NarI NgoMIV NsiI NspI PasI
PflMI PshAI SacII SfoI StuI StyI TaqII TstI
Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BcgI
BciVI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI ClaI
CspCI DraIII EagI EarI EciI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XcmI XhoI
XmaI XmnI ZraI