Restriction map for
pFN21AE9256
(Linear) (Six-base) MAPSORT of: KIEE9256.seq Check: 7161 from: 1 to: 2154
KIEE9256.orf
With 182 enzymes: SgfI *
July 3, 2012 19:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 812 1809 2087 2154
Size: 812 997 278 67
Fragments arranged by size:
997 812 278 67
AccI GT'mk_AC
Cuts at: 0 299 2154
Size: 299 1855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 505 796 1450 1459 1831 1858 2118
Size: 322 183 291 654 9 372 27 260
Cuts at: 2118 2154
Size: 36
Fragments arranged by size:
654 372 322 291 260 183 36 27
9
AleI CACnn'nnGTG
Cuts at: 0 1651 2154
Size: 1651 503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 448 2154
Size: 448 1706
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1454 1486 2026 2058 2154
Size: 1454 32 540 32 96
Fragments arranged by size:
1454 540 96 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 567 693 1104 1377 1668 2031 2154
Size: 567 126 411 273 291 363 123
Fragments arranged by size:
567 411 363 291 273 126 123
ApaLI G'TGCA_C
Cuts at: 0 1333 2074 2154
Size: 1333 741 80
Fragments arranged by size:
1333 741 80
AvaI C'yCGr_G
Cuts at: 0 242 1076 2010 2125 2154
Size: 242 834 934 115 29
Fragments arranged by size:
934 834 242 115 29
AvrII C'CTAG_G
Cuts at: 0 757 2154
Size: 757 1397
BanI G'GyrC_C
Cuts at: 0 872 1744 2084 2154
Size: 872 872 340 70
Fragments arranged by size:
872 872 340 70
BanII G_rGCy'C
Cuts at: 0 243 317 1118 1590 1721 2066 2132 2154
Size: 243 74 801 472 131 345 66 22
Fragments arranged by size:
801 472 345 243 131 74 66 22
BbeI G_GCGC'C
Cuts at: 0 876 2088 2154
Size: 876 1212 66
Fragments arranged by size:
1212 876 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 11 1001 2154
Size: 11 990 1153
Fragments arranged by size:
1153 990 11
BbvCI CC'TCA_GC
Cuts at: 0 1313 2110 2154
Size: 1313 797 44
Fragments arranged by size:
1313 797 44
BglII A'GATC_T
Cuts at: 0 43 2154
Size: 43 2111
BlpI GC'TnA_GC
Cuts at: 0 1361 2154
Size: 1361 793
Bme1580I G_kGCm'C
Cuts at: 0 1337 2078 2154
Size: 1337 741 76
Fragments arranged by size:
1337 741 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 2154
Size: 99 2055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 274 403 568 616 703 820 934
Size: 172 102 129 165 48 87 117 114
Cuts at: 934 974 1105 1174 1399 1438 1464 1564 1585
Size: 40 131 69 225 39 26 100 21
Cuts at: 1585 1597 2053 2154
Size: 12 456 101
Fragments arranged by size:
456 225 172 165 131 129 117 114
102 101 100 87 69 48 40 39
26 21 12
Bpu10I CC'TnA_GC
Cuts at: 0 976 1313 1501 2110 2154
Size: 976 337 188 609 44
Fragments arranged by size:
976 609 337 188 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1783 1837 2154
Size: 1783 54 317
Fragments arranged by size:
1783 317 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 463 814 1156 2154
Size: 463 351 342 998
Fragments arranged by size:
998 463 351 342
BsaBI GATnn'nnATC
Cuts at: 0 1924 2154
Size: 1924 230
BsaHI Gr'CG_yC
Cuts at: 0 873 2085 2154
Size: 873 1212 69
Fragments arranged by size:
1212 873 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1733 1763 1927 1957 2026 2056 2154
Size: 1733 30 164 30 69 30 98
Fragments arranged by size:
1733 164 98 69 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 805 1126 1159 1396 1501 1531 1561
Size: 376 429 321 33 237 105 30 30
Cuts at: 1561 1754 2154
Size: 193 400
Fragments arranged by size:
429 400 376 321 237 193 105 33
30 30
BseYI C'CCAG_C
Cuts at: 0 867 947 1286 1386 1752 2154
Size: 867 80 339 100 366 402
Fragments arranged by size:
867 402 366 339 100 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1612 1654 2154
Size: 1345 267 42 500
Fragments arranged by size:
1345 500 267 42
BsiEI CG_ry'CG
Cuts at: 0 303 1274 2154
Size: 303 971 880
Fragments arranged by size:
971 880 303
BsiHKAI G_wGCw'C
Cuts at: 0 317 1118 1337 1590 1973 2066 2078 2132
Size: 317 801 219 253 383 93 12 54
Cuts at: 2132 2154
Size: 22
Fragments arranged by size:
801 383 317 253 219 93 54 22
12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 886 2154
Size: 886 1268
Bsp1286I G_dGCh'C
Cuts at: 0 243 317 1118 1337 1590 1721 1973 2066
Size: 243 74 801 219 253 131 252 93
Cuts at: 2066 2078 2132 2154
Size: 12 54 22
Fragments arranged by size:
801 253 252 243 219 131 93 74
54 22 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 593 812 1113 1809 2087 2154
Size: 593 219 301 696 278 67
Fragments arranged by size:
696 593 301 278 219 67
BsrBI CCG'CTC
Cuts at: 0 204 345 1221 1533 1581 2154
Size: 204 141 876 312 48 573
Fragments arranged by size:
876 573 312 204 141 48
BsrFI r'CCGG_y
Cuts at: 0 1909 1946 2154
Size: 1909 37 208
Fragments arranged by size:
1909 208 37
BssHII G'CGCG_C
Cuts at: 0 797 1725 2154
Size: 797 928 429
Fragments arranged by size:
928 797 429
BssSI C'ACGA_G
Cuts at: 0 1168 1672 2154
Size: 1168 504 482
Fragments arranged by size:
1168 504 482
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 567 1104 1332 1668 1895 2154
Size: 144 423 537 228 336 227 259
Fragments arranged by size:
537 423 336 259 228 227 144
BstBI TT'CG_AA
Cuts at: 0 178 2154
Size: 178 1976
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1011 1653 1800 2154
Size: 1011 642 147 354
Fragments arranged by size:
1011 642 354 147
BstYI r'GATC_y
Cuts at: 0 43 170 494 678 1874 2154
Size: 43 127 324 184 1196 280
Fragments arranged by size:
1196 324 280 184 127 43
BtgI C'CryG_G
Cuts at: 0 23 968 1730 1931 2054 2147 2154
Size: 23 945 762 201 123 93 7
Fragments arranged by size:
945 762 201 123 93 23 7
BtsI GCAGTG_nn'
Cuts at: 0 403 514 2154
Size: 403 111 1640
Fragments arranged by size:
1640 403 111
DraIII CAC_nnn'GTG
Cuts at: 0 2055 2154
Size: 2055 99
EaeI y'GGCC_r
Cuts at: 0 932 1131 1271 1655 2154
Size: 932 199 140 384 499
Fragments arranged by size:
932 499 384 199 140
EagI C'GGCC_G
Cuts at: 0 1271 2154
Size: 1271 883
EarI CTCTTCn'nnn_
Cuts at: 0 836 2137 2154
Size: 836 1301 17
Fragments arranged by size:
1301 836 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 373 1507 1711 2154
Size: 373 1134 204 443
Fragments arranged by size:
1134 443 373 204
EcoICRI GAG'CTC
Cuts at: 0 315 1116 1588 2064 2130 2154
Size: 315 801 472 476 66 24
Fragments arranged by size:
801 476 472 315 66 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 274 322 403 505 568 616 703
Size: 172 102 48 81 102 63 48 87
Cuts at: 703 796 820 934 974 1105 1174 1399 1438
Size: 93 24 114 40 131 69 225 39
Cuts at: 1438 1450 1459 1464 1564 1585 1597 1831 1858
Size: 12 9 5 100 21 12 234 27
Cuts at: 1858 2053 2118 2154
Size: 195 65 36
Fragments arranged by size:
234 225 195 172 131 114 102 102
100 93 87 81 69 65 63 48
48 40 39 36 27 24 21 12
12 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 193 1672 2154
Size: 193 1479 482
Fragments arranged by size:
1479 482 193
EcoO109I rG'GnC_Cy
Cuts at: 0 1200 1247 1374 2154
Size: 1200 47 127 780
Fragments arranged by size:
1200 780 127 47
HaeII r_GCGC'y
Cuts at: 0 546 876 1061 1076 1177 1349 1668 2088
Size: 546 330 185 15 101 172 319 420
Cuts at: 2088 2154
Size: 66
Fragments arranged by size:
546 420 330 319 185 172 101 66
15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 663 695 1344 1376 1733 1765 2154
Size: 663 32 649 32 357 32 389
Fragments arranged by size:
663 649 389 357 32 32 32
HincII GTy'rAC
Cuts at: 0 300 2154
Size: 300 1854
KasI G'GCGC_C
Cuts at: 0 872 2084 2154
Size: 872 1212 70
Fragments arranged by size:
1212 872 70
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 1121 2154
Size: 533 588 1033
Fragments arranged by size:
1033 588 533
MscI TGG'CCA
Cuts at: 0 934 1657 2154
Size: 934 723 497
Fragments arranged by size:
934 723 497
MslI CAynn'nnrTG
Cuts at: 0 196 1651 2071 2154
Size: 196 1455 420 83
Fragments arranged by size:
1455 420 196 83
MspA1I CmG'CkG
Cuts at: 0 150 381 579 783 1104 1130 1137 1290
Size: 150 231 198 204 321 26 7 153
Cuts at: 1290 1365 1575 1689 1848 2154
Size: 75 210 114 159 306
Fragments arranged by size:
321 306 231 210 204 198 159 153
150 114 75 26 7
NarI GG'CG_CC
Cuts at: 0 873 2085 2154
Size: 873 1212 69
Fragments arranged by size:
1212 873 69
NcoI C'CATG_G
Cuts at: 0 23 968 1730 2154
Size: 23 945 762 424
Fragments arranged by size:
945 762 424 23
NspI r_CATG'y
Cuts at: 0 147 1305 1416 2154
Size: 147 1158 111 738
Fragments arranged by size:
1158 738 147 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 969 1140 2148 2154
Size: 969 171 1008 6
Fragments arranged by size:
1008 969 171 6
PfoI T'CCnGG_A
Cuts at: 0 623 836 1877 2154
Size: 623 213 1041 277
Fragments arranged by size:
1041 623 277 213
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2026 2058 2154
Size: 2026 32 96
Fragments arranged by size:
2026 96 32
PpuMI rG'GwC_Cy
Cuts at: 0 1200 1247 2154
Size: 1200 47 907
Fragments arranged by size:
1200 907 47
PspXI vC'TCGA_Gb
Cuts at: 0 242 2125 2154
Size: 242 1883 29
Fragments arranged by size:
1883 242 29
PstI C_TGCA'G
Cuts at: 0 125 509 572 860 1025 1109 1595 1694
Size: 125 384 63 288 165 84 486 99
Cuts at: 1694 2154
Size: 460
Fragments arranged by size:
486 460 384 288 165 125 99 84
63
PvuII CAG'CTG
Cuts at: 0 150 381 579 783 1104 1137 1290 1365
Size: 150 231 198 204 321 33 153 75
Cuts at: 1365 1575 1689 2154
Size: 210 114 465
Fragments arranged by size:
465 321 231 210 204 198 153 150
114 75 33
SacI G_AGCT'C
Cuts at: 0 317 1118 1590 2066 2132 2154
Size: 317 801 472 476 66 22
Fragments arranged by size:
801 476 472 317 66 22
SalI G'TCGA_C
Cuts at: 0 298 2154
Size: 298 1856
SapI GCTCTTCn'nnn_
Cuts at: 0 836 2137 2154
Size: 836 1301 17
Fragments arranged by size:
1301 836 17
ScaI AGT'ACT
Cuts at: 0 1279 2154
Size: 1279 875
SfcI C'TryA_G
Cuts at: 0 121 505 568 856 1021 1105 1591 1690
Size: 121 384 63 288 165 84 486 99
Cuts at: 1690 2154
Size: 464
Fragments arranged by size:
486 464 384 288 165 121 99 84
63
SfoI GGC'GCC
Cuts at: 0 874 2086 2154
Size: 874 1212 68
Fragments arranged by size:
1212 874 68
SmaI CCC'GGG
Cuts at: 0 1078 2154
Size: 1078 1076
SmlI C'TyrA_G
Cuts at: 0 242 1762 1852 2125 2154
Size: 242 1520 90 273 29
Fragments arranged by size:
1520 273 242 90 29
SphI G_CATG'C
Cuts at: 0 147 1305 2154
Size: 147 1158 849
Fragments arranged by size:
1158 849 147
StuI AGG'CCT
Cuts at: 0 1894 2154
Size: 1894 260
StyI C'CwwG_G
Cuts at: 0 23 757 968 1214 1730 1793 2154
Size: 23 734 211 246 516 63 361
Fragments arranged by size:
734 516 361 246 211 63 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1988 2154
Size: 1988 166
TatI w'GTAC_w
Cuts at: 0 1277 2154
Size: 1277 877
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 493 525 2154
Size: 493 32 1629
Fragments arranged by size:
1629 493 32
XhoI C'TCGA_G
Cuts at: 0 242 2125 2154
Size: 242 1883 29
Fragments arranged by size:
1883 242 29
XmaI C'CCGG_G
Cuts at: 0 1076 2154
Size: 1076 1078
XmnI GAAnn'nnTTC
Cuts at: 0 55 2154
Size: 55 2099
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AloI AlwNI ApaLI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BglII
BlpI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI BtgI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NspI
PflMI PfoI PpiI PpuMI PspXI PstI PvuII SacI
SalI SapI ScaI SfcI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
ApaI ApoI AscI AseI AsiSI BaeI BamHI BcgI
BciVI BclI BfrBI BglI BmgBI BmrI BmtI BsaAI
BsaWI BsiWI BsmI BspEI BspHI BsrDI BsrGI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SanDI SbfI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XcmI ZraI