Restriction map for
pFN21AE9254
(Linear) (Six-base) MAPSORT of: KIEE9254.seq Check: 9518 from: 1 to: 2244
KIEE9254.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 411 564 2244
Size: 411 153 1680
Fragments arranged by size:
1680 411 153
AccI GT'mk_AC
Cuts at: 0 1822 1947 2244
Size: 1822 125 297
Fragments arranged by size:
1822 297 125
AclI AA'CG_TT
Cuts at: 0 859 2244
Size: 859 1385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 2244
Size: 1414 830
AflII C'TTAA_G
Cuts at: 0 732 2244
Size: 732 1512
AflIII A'CryG_T
Cuts at: 0 525 1394 1984 2180 2244
Size: 525 869 590 196 64
Fragments arranged by size:
869 590 525 196 64
AleI CACnn'nnGTG
Cuts at: 0 142 2244
Size: 142 2102
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1554 2244
Size: 1554 690
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2014 2046 2244
Size: 2014 32 198
Fragments arranged by size:
2014 198 32
AlwNI CAG_nnn'CTG
Cuts at: 0 54 105 2244
Size: 54 51 2139
Fragments arranged by size:
2139 54 51
ApoI r'AATT_y
Cuts at: 0 191 1747 2244
Size: 191 1556 497
Fragments arranged by size:
1556 497 191
AvrII C'CTAG_G
Cuts at: 0 1509 2244
Size: 1509 735
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 567 600 2244
Size: 567 33 1644
Fragments arranged by size:
1644 567 33
BamHI G'GATC_C
Cuts at: 0 645 2244
Size: 645 1599
BanI G'GyrC_C
Cuts at: 0 1538 2095 2244
Size: 1538 557 149
Fragments arranged by size:
1538 557 149
BanII G_rGCy'C
Cuts at: 0 958 1957 2071 2244
Size: 958 999 114 173
Fragments arranged by size:
999 958 173 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 47 210 2244
Size: 47 163 2034
Fragments arranged by size:
2034 163 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1159 1193 2244
Size: 1159 34 1051
Fragments arranged by size:
1159 1051 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 568 2244
Size: 568 1676
BclI T'GATC_A
Cuts at: 0 1314 2244
Size: 1314 930
BglI GCCn_nnn'nGGC
Cuts at: 0 1433 2244
Size: 1433 811
BglII A'GATC_T
Cuts at: 0 1519 2244
Size: 1519 725
BmgBI CAC'GTC
Cuts at: 0 36 365 2183 2244
Size: 36 329 1818 61
Fragments arranged by size:
1818 329 61 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1960 2244
Size: 1960 284
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 327 2244
Size: 327 1917
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 867 1158 2244
Size: 576 291 291 1086
Fragments arranged by size:
1086 576 291 291
Bpu10I CC'TnA_GC
Cuts at: 0 64 302 396 2244
Size: 64 238 94 1848
Fragments arranged by size:
1848 238 94 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 2126 2244
Size: 706 1420 118
Fragments arranged by size:
1420 706 118
BsaAI yAC'GTr
Cuts at: 0 526 2244
Size: 526 1718
BsaBI GATnn'nnATC
Cuts at: 0 1303 2244
Size: 1303 941
BsaHI Gr'CG_yC
Cuts at: 0 175 2076 2244
Size: 175 1901 168
Fragments arranged by size:
1901 175 168
BsaWI w'CCGG_w
Cuts at: 0 2071 2244
Size: 2071 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 685 715 865 895 1156 1186 2244
Size: 685 30 150 30 261 30 1058
Fragments arranged by size:
1058 685 261 150 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 234 903 966 2186 2244
Size: 234 669 63 1220 58
Fragments arranged by size:
1220 669 234 63 58
BseYI C'CCAG_C
Cuts at: 0 3 612 1672 2244
Size: 3 609 1060 572
Fragments arranged by size:
1060 609 572 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 518 787 1956 2244
Size: 500 18 269 1169 288
Fragments arranged by size:
1169 500 288 269 18
BsiHKAI G_wGCw'C
Cuts at: 0 829 958 2244
Size: 829 129 1286
Fragments arranged by size:
1286 829 129
BsmBI CGTCTCn'nnnn_
Cuts at: 0 166 2244
Size: 166 2078
Bsp1286I G_dGCh'C
Cuts at: 0 829 958 1957 2071 2244
Size: 829 129 999 114 173
Fragments arranged by size:
999 829 173 129 114
BspEI T'CCGG_A
Cuts at: 0 2071 2244
Size: 2071 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 411 490 564 1522 2244
Size: 411 79 74 958 722
Fragments arranged by size:
958 722 411 79 74
BsrDI GCAATG_nn'
Cuts at: 0 774 2244
Size: 774 1470
BsrFI r'CCGG_y
Cuts at: 0 815 2244
Size: 815 1429
BsrGI T'GTAC_A
Cuts at: 0 1397 2244
Size: 1397 847
BssHII G'CGCG_C
Cuts at: 0 15 2244
Size: 15 2229
BssSI C'ACGA_G
Cuts at: 0 1994 2004 2016 2244
Size: 1994 10 12 228
Fragments arranged by size:
1994 228 12 10
BstEII G'GTnAC_C
Cuts at: 0 607 2244
Size: 607 1637
BstXI CCAn_nnnn'nTGG
Cuts at: 0 672 2244
Size: 672 1572
BstYI r'GATC_y
Cuts at: 0 233 645 1110 1519 2244
Size: 233 412 465 409 725
Fragments arranged by size:
725 465 412 409 233
BtgI C'CryG_G
Cuts at: 0 20 257 1275 1713 2244
Size: 20 237 1018 438 531
Fragments arranged by size:
1018 531 438 237 20
BtsI GCAGTG_nn'
Cuts at: 0 413 2244
Size: 413 1831
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 167 202 2244
Size: 167 35 2042
Fragments arranged by size:
2042 167 35
DraI TTT'AAA
Cuts at: 0 1058 2244
Size: 1058 1186
DrdI GACnn_nn'nnGTC
Cuts at: 0 2119 2244
Size: 2119 125
EaeI y'GGCC_r
Cuts at: 0 155 2244
Size: 155 2089
EarI CTCTTCn'nnn_
Cuts at: 0 371 2151 2159 2192 2244
Size: 371 1780 8 33 52
Fragments arranged by size:
1780 371 52 33 8
EcoICRI GAG'CTC
Cuts at: 0 956 2244
Size: 956 1288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 867 1158 1414 2244
Size: 576 291 291 256 830
Fragments arranged by size:
830 576 291 291 256
EcoNI CCTnn'n_nnAGG
Cuts at: 0 992 2244
Size: 992 1252
EcoO109I rG'GnC_Cy
Cuts at: 0 298 1408 2244
Size: 298 1110 836
Fragments arranged by size:
1110 836 298
EcoRV GAT'ATC
Cuts at: 0 1852 2244
Size: 1852 392
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 984 1093 1514 2244
Size: 984 109 421 730
Fragments arranged by size:
984 730 421 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 685 717 1082 1114 2010 2042
Size: 349 32 304 32 365 32 896 32
Cuts at: 2042 2167 2199 2244
Size: 125 32 45
Fragments arranged by size:
896 365 349 304 125 45 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1086 2244
Size: 1086 1158
HindIII A'AGCT_T
Cuts at: 0 1070 1756 2244
Size: 1070 686 488
Fragments arranged by size:
1070 686 488
MfeI C'AATT_G
Cuts at: 0 1964 2244
Size: 1964 280
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1099 2244
Size: 1099 1145
MscI TGG'CCA
Cuts at: 0 157 2244
Size: 157 2087
MslI CAynn'nnrTG
Cuts at: 0 142 670 1221 1393 1983 2244
Size: 142 528 551 172 590 261
Fragments arranged by size:
590 551 528 261 172 142
MspA1I CmG'CkG
Cuts at: 0 51 102 259 537 2244
Size: 51 51 157 278 1707
Fragments arranged by size:
1707 278 157 51 51
NaeI GCC'GGC
Cuts at: 0 817 2244
Size: 817 1427
NcoI C'CATG_G
Cuts at: 0 20 1275 2244
Size: 20 1255 969
Fragments arranged by size:
1255 969 20
NgoMIV G'CCGG_C
Cuts at: 0 815 2244
Size: 815 1429
NspI r_CATG'y
Cuts at: 0 99 1398 1988 2244
Size: 99 1299 590 256
Fragments arranged by size:
1299 590 256 99
PasI CC'CwG_GG
Cuts at: 0 1929 2064 2091 2244
Size: 1929 135 27 153
Fragments arranged by size:
1929 153 135 27
PciI A'CATG_T
Cuts at: 0 1394 1984 2244
Size: 1394 590 260
Fragments arranged by size:
1394 590 260
PflMI CCAn_nnn'nTGG
Cuts at: 0 641 2244
Size: 641 1603
PfoI T'CCnGG_A
Cuts at: 0 236 883 1373 2244
Size: 236 647 490 871
Fragments arranged by size:
871 647 490 236
PpuMI rG'GwC_Cy
Cuts at: 0 298 1408 2244
Size: 298 1110 836
Fragments arranged by size:
1110 836 298
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2170 2202 2244
Size: 2170 32 42
Fragments arranged by size:
2170 42 32
PstI C_TGCA'G
Cuts at: 0 59 291 408 583 2244
Size: 59 232 117 175 1661
Fragments arranged by size:
1661 232 175 117 59
PvuII CAG'CTG
Cuts at: 0 51 2244
Size: 51 2193
SacI G_AGCT'C
Cuts at: 0 958 2244
Size: 958 1286
SacII CC_GC'GG
Cuts at: 0 260 2244
Size: 260 1984
SapI GCTCTTCn'nnn_
Cuts at: 0 2151 2244
Size: 2151 93
SfcI C'TryA_G
Cuts at: 0 55 287 404 579 1823 2244
Size: 55 232 117 175 1244 421
Fragments arranged by size:
1244 421 232 175 117 55
SmlI C'TyrA_G
Cuts at: 0 732 2244
Size: 732 1512
SphI G_CATG'C
Cuts at: 0 99 2244
Size: 99 2145
SspI AAT'ATT
Cuts at: 0 1333 1652 2244
Size: 1333 319 592
Fragments arranged by size:
1333 592 319
StyI C'CwwG_G
Cuts at: 0 20 959 1275 1509 2244
Size: 20 939 316 234 735
Fragments arranged by size:
939 735 316 234 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1686 2083 2244
Size: 1686 397 161
Fragments arranged by size:
1686 397 161
TatI w'GTAC_w
Cuts at: 0 116 1397 2244
Size: 116 1281 847
Fragments arranged by size:
1281 847 116
XmnI GAAnn'nnTTC
Cuts at: 0 1641 2244
Size: 1641 603
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AleI AlfI
AloI AlwNI ApoI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI
BstYI BtgI BtsI CspCI DraI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PpuMI PsrI
PstI PvuII SacI SacII SapI SfcI SmlI SphI
SspI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI ApaI ApaLI AscI
AseI AsiSI AvaI BbeI BbvCI BfrBI BlpI Bme1580I
BmtI BpuEI BsiEI BsiWI BsmI BspHI BsrBI BstAPI
BstBI BstZ17I Bsu36I BtgZI ClaI DraIII EagI EciI
EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI