(Linear) (Six-base) MAPSORT of: KIEE9252.seq  Check: 8574  from: 1  to: 264



KIEE9252.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:38  ..

AccI GT'mk_AC

Cuts at:      0      10     264 
   Size:         10     254
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      66     264 
   Size:         66     198
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     264 
   Size:        124     140
 
BtgI C'CryG_G

Cuts at:      0      54     264 
   Size:         54     210
 
ClaI AT'CG_AT

Cuts at:      0     156     264 
   Size:        156     108
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     124     264 
   Size:        124     140
 
EaeI y'GGCC_r

Cuts at:      0     227     264 
   Size:        227      37
 
EarI CTCTTCn'nnn_

Cuts at:      0     161     247     264 
   Size:        161      86      17

  Fragments arranged by size: 

                 161      86      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     264 
   Size:        124     140
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      90     264 
   Size:         90     174
 
HaeII r_GCGC'y

Cuts at:      0       7     264 
   Size:          7     257
 
HincII GTy'rAC

Cuts at:      0      11     264 
   Size:         11     253
 
NspI r_CATG'y

Cuts at:      0     223     264 
   Size:        223      41
 
PfoI T'CCnGG_A

Cuts at:      0     172     264 
   Size:        172      92
 
PshAI GACnn'nnGTC

Cuts at:      0      16     264 
   Size:         16     248
 
SalI G'TCGA_C

Cuts at:      0       9     264 
   Size:          9     255
 
SapI GCTCTTCn'nnn_

Cuts at:      0     247     264 
   Size:        247      17
 
TatI w'GTAC_w

Cuts at:      0     222     264 
   Size:        222      42
 

 Enzymes that do cut:

     AccI     BbsI     BpmI     BtgI     ClaI     DrdI     EaeI     EarI
  Eco57MI     FalI    HaeII   HincII     NspI     PfoI    PshAI     SalI
     SapI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI    CspCI     DraI   DraIII     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI