(Linear) (Six-base) MAPSORT of: KIEE9250.seq  Check: 9668  from: 1  to: 1059



KIEE9250.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     319     351    1059 
   Size:         94     225      32     708

  Fragments arranged by size: 

                 708     225      94      32
 
AflII C'TTAA_G

Cuts at:      0     601    1059 
   Size:        601     458
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     960    1059 
   Size:        960      99
 
AlwNI CAG_nnn'CTG

Cuts at:      0     534    1059 
   Size:        534     525
 
ApoI r'AATT_y

Cuts at:      0     117     315     329     793     820    1059 
   Size:        117     198      14     464      27     239

  Fragments arranged by size: 

                 464     239     198     117      27      14
 
AvaI C'yCGr_G

Cuts at:      0    1002    1059 
   Size:       1002      57
 
BamHI G'GATC_C

Cuts at:      0     950    1059 
   Size:        950     109
 
BbvCI CC'TCA_GC

Cuts at:      0     502    1059 
   Size:        502     557
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1058    1059 
   Size:       1058       1
 
BmgBI CAC'GTC

Cuts at:      0     488    1059 
   Size:        488     571
 
BmtI G_CTAG'C

Cuts at:      0     548    1059 
   Size:        548     511
 
Bpu10I CC'TnA_GC

Cuts at:      0     502     924    1059 
   Size:        502     422     135

  Fragments arranged by size: 

                 502     422     135
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740    1059 
   Size:        740     319
 
BsaAI yAC'GTr

Cuts at:      0     453    1059 
   Size:        453     606
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     394     483    1059 
   Size:        394      89     576

  Fragments arranged by size: 

                 576     394      89
 
BsiWI C'GTAC_G

Cuts at:      0     476    1059 
   Size:        476     583
 
BsmI GAATG_Cn'

Cuts at:      0     390    1059 
   Size:        390     669
 
BspHI T'CATG_A

Cuts at:      0     714    1059 
   Size:        714     345
 
BstBI TT'CG_AA

Cuts at:      0     399    1059 
   Size:        399     660
 
BstYI r'GATC_y

Cuts at:      0     950    1059 
   Size:        950     109
 
DraI TTT'AAA

Cuts at:      0     312     618     836    1059 
   Size:        312     306     218     223

  Fragments arranged by size: 

                 312     306     223     218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     319     351    1059 
   Size:         94     225      32     708

  Fragments arranged by size: 

                 708     225      94      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0     992     999    1059 
   Size:        992       7      60

  Fragments arranged by size: 

                 992      60       7
 
HaeII r_GCGC'y

Cuts at:      0     535    1059 
   Size:        535     524
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108    1059 
   Size:         76      32     951

  Fragments arranged by size: 

                 951      76      32
 
HincII GTy'rAC

Cuts at:      0     288    1059 
   Size:        288     771
 
HindIII A'AGCT_T

Cuts at:      0     808    1059 
   Size:        808     251
 
MfeI C'AATT_G

Cuts at:      0     539    1059 
   Size:        539     520
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     147     768    1059 
   Size:        147     621     291

  Fragments arranged by size: 

                 621     291     147
 
MslI CAynn'nnrTG

Cuts at:      0     164     217    1059 
   Size:        164      53     842

  Fragments arranged by size: 

                 842     164      53
 
MspA1I CmG'CkG

Cuts at:      0     934    1010    1059 
   Size:        934      76      49

  Fragments arranged by size: 

                 934      76      49
 
NheI G'CTAG_C

Cuts at:      0     544    1059 
   Size:        544     515
 
PpuMI rG'GwC_Cy

Cuts at:      0     992     999    1059 
   Size:        992       7      60

  Fragments arranged by size: 

                 992      60       7
 
PstI C_TGCA'G

Cuts at:      0     110    1059 
   Size:        110     949
 
PvuII CAG'CTG

Cuts at:      0    1010    1059 
   Size:       1010      49
 
SanDI GG'GwC_CC

Cuts at:      0     992    1059 
   Size:        992      67
 
SexAI A'CCwGG_T

Cuts at:      0     273    1059 
   Size:        273     786
 
SfcI C'TryA_G

Cuts at:      0     106     140     664    1059 
   Size:        106      34     524     395

  Fragments arranged by size: 

                 524     395     106      34
 
SmlI C'TyrA_G

Cuts at:      0     601     755    1059 
   Size:        601     154     304

  Fragments arranged by size: 

                 601     304     154
 
StuI AGG'CCT

Cuts at:      0      79    1059 
   Size:         79     980
 
StyI C'CwwG_G

Cuts at:      0     678    1059 
   Size:        678     381
 
TatI w'GTAC_w

Cuts at:      0     419     437     458    1059 
   Size:        419      18      21     601

  Fragments arranged by size: 

                 601     419      21      18
 

 Enzymes that do cut:

     AcuI    AflII     AlfI    AlwNI     ApoI     AvaI    BamHI    BbvCI
    BciVI    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI    BseRI    BsiWI
     BsmI    BspHI    BstBI    BstYI     DraI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII  HindIII     MfeI     MmeI     MslI   MspA1I     NheI
    PpuMI     PstI    PvuII    SanDI    SexAI     SfcI     SmlI     StuI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI    BanII     BbeI     BbsI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI