Restriction map for
pFN21AE9222
(Linear) (Six-base) MAPSORT of: KIEE9222.seq Check: 206 from: 1 to: 3135
KIEE9222.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AccI GT'mk_AC
Cuts at: 0 327 698 2393 3135
Size: 327 371 1695 742
Fragments arranged by size:
1695 742 371 327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 248 289 637 914 937 1406 1535
Size: 151 97 41 348 277 23 469 129
Cuts at: 1535 1618 2153 2362 2539 3135
Size: 83 535 209 177 596
Fragments arranged by size:
596 535 469 348 277 209 177 151
129 97 83 41 23
AflIII A'CryG_T
Cuts at: 0 508 1190 3135
Size: 508 682 1945
Fragments arranged by size:
1945 682 508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2972 3135
Size: 2972 163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2837 2869 3135
Size: 2837 32 266
Fragments arranged by size:
2837 266 32
ApaI G_GGCC'C
Cuts at: 0 2711 3135
Size: 2711 424
ApaLI G'TGCA_C
Cuts at: 0 1426 3135
Size: 1426 1709
ApoI r'AATT_y
Cuts at: 0 344 553 691 772 1142 1159 1259 1348
Size: 344 209 138 81 370 17 100 89
Cuts at: 1348 1614 3135
Size: 266 1521
Fragments arranged by size:
1521 370 344 266 209 138 100 89
81 17
AseI AT'TA_AT
Cuts at: 0 1943 2831 2876 3135
Size: 1943 888 45 259
Fragments arranged by size:
1943 888 259 45
AvaI C'yCGr_G
Cuts at: 0 54 3135
Size: 54 3081
AvrII C'CTAG_G
Cuts at: 0 2979 3135
Size: 2979 156
BanI G'GyrC_C
Cuts at: 0 1743 3135
Size: 1743 1392
BanII G_rGCy'C
Cuts at: 0 197 2711 3135
Size: 197 2514 424
Fragments arranged by size:
2514 424 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 567 1550 1624 3135
Size: 567 983 74 1511
Fragments arranged by size:
1511 983 567 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2738 2772 3135
Size: 2738 34 363
Fragments arranged by size:
2738 363 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 956 3135
Size: 956 2179
BclI T'GATC_A
Cuts at: 0 2808 2849 3135
Size: 2808 41 286
Fragments arranged by size:
2808 286 41
BfrBI ATG'CAT
Cuts at: 0 378 3135
Size: 378 2757
BglI GCCn_nnn'nGGC
Cuts at: 0 37 486 3135
Size: 37 449 2649
Fragments arranged by size:
2649 449 37
BglII A'GATC_T
Cuts at: 0 363 3135
Size: 363 2772
Bme1580I G_kGCm'C
Cuts at: 0 1430 2711 3135
Size: 1430 1281 424
Fragments arranged by size:
1430 1281 424
BmrI ACTGGGnnnn_n'
Cuts at: 0 191 3135
Size: 191 2944
BmtI G_CTAG'C
Cuts at: 0 820 3135
Size: 820 2315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 480 1064 2307 2785 3135
Size: 352 128 584 1243 478 350
Fragments arranged by size:
1243 584 478 352 350 128
Bpu10I CC'TnA_GC
Cuts at: 0 1299 3135
Size: 1299 1836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1086 1825 2014 2492 3135
Size: 1086 739 189 478 643
Fragments arranged by size:
1086 739 643 478 189
BsaI GGTCTCn'nnnn_
Cuts at: 0 925 2341 3135
Size: 925 1416 794
Fragments arranged by size:
1416 925 794
BsaBI GATnn'nnATC
Cuts at: 0 2462 3135
Size: 2462 673
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 770 800 3135
Size: 770 30 2335
Fragments arranged by size:
2335 770 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 477 1621 1659 2092 2124 3135
Size: 477 1144 38 433 32 1011
Fragments arranged by size:
1144 1011 477 433 38 32
BseYI C'CCAG_C
Cuts at: 0 1305 3135
Size: 1305 1830
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 3135
Size: 886 2249
BsiEI CG_ry'CG
Cuts at: 0 32 3135
Size: 32 3103
BsiHKAI G_wGCw'C
Cuts at: 0 1430 2938 3135
Size: 1430 1508 197
Fragments arranged by size:
1508 1430 197
BsmI GAATG_Cn'
Cuts at: 0 98 733 808 2305 2778 2861 3135
Size: 98 635 75 1497 473 83 274
Fragments arranged by size:
1497 635 473 274 98 83 75
Bsp1286I G_dGCh'C
Cuts at: 0 197 1430 2711 2938 3135
Size: 197 1233 1281 227 197
Fragments arranged by size:
1281 1233 227 197 197
BspHI T'CATG_A
Cuts at: 0 285 1773 2918 3135
Size: 285 1488 1145 217
Fragments arranged by size:
1488 1145 285 217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1374 3135
Size: 1374 1761
BsrBI CCG'CTC
Cuts at: 0 1320 2279 3135
Size: 1320 959 856
Fragments arranged by size:
1320 959 856
BsrDI GCAATG_nn'
Cuts at: 0 314 2691 3135
Size: 314 2377 444
Fragments arranged by size:
2377 444 314
BsrFI r'CCGG_y
Cuts at: 0 31 3135
Size: 31 3104
BsrGI T'GTAC_A
Cuts at: 0 1193 1937 3135
Size: 1193 744 1198
Fragments arranged by size:
1198 1193 744
BstAPI GCAn_nnn'nTGC
Cuts at: 0 391 3135
Size: 391 2744
BstBI TT'CG_AA
Cuts at: 0 558 1215 1236 3135
Size: 558 657 21 1899
Fragments arranged by size:
1899 657 558 21
BstYI r'GATC_y
Cuts at: 0 363 717 3006 3135
Size: 363 354 2289 129
Fragments arranged by size:
2289 363 354 129
BtgI C'CryG_G
Cuts at: 0 48 998 1562 3135
Size: 48 950 564 1573
Fragments arranged by size:
1573 950 564 48
BtsI GCAGTG_nn'
Cuts at: 0 1428 3135
Size: 1428 1707
DraI TTT'AAA
Cuts at: 0 1676 3135
Size: 1676 1459
DraIII CAC_nnn'GTG
Cuts at: 0 1041 2299 3135
Size: 1041 1258 836
Fragments arranged by size:
1258 1041 836
EaeI y'GGCC_r
Cuts at: 0 29 45 478 939 3135
Size: 29 16 433 461 2196
Fragments arranged by size:
2196 461 433 29 16
EagI C'GGCC_G
Cuts at: 0 29 3135
Size: 29 3106
EarI CTCTTCn'nnn_
Cuts at: 0 354 956 1485 2084 2184 2269 2546 3135
Size: 354 602 529 599 100 85 277 589
Fragments arranged by size:
602 599 589 529 354 277 100 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 248 289 352 480 637 914 937
Size: 151 97 41 63 128 157 277 23
Cuts at: 937 1064 1406 1535 1618 2153 2307 2362 2539
Size: 127 342 129 83 535 154 55 177
Cuts at: 2539 2785 3135
Size: 246 350
Fragments arranged by size:
535 350 342 277 246 177 157 154
151 129 128 127 97 83 63 55
41 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1738 3135
Size: 1738 1397
EcoO109I rG'GnC_Cy
Cuts at: 0 105 2707 2708 3135
Size: 105 2602 1 427
Fragments arranged by size:
2602 427 105 1
EcoRI G'AATT_C
Cuts at: 0 772 3135
Size: 772 2363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 319 2671 3135
Size: 319 2352 464
Fragments arranged by size:
2352 464 319
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 554 586 1435 1467 1536 1568 1984 2016
Size: 554 32 849 32 69 32 416 32
Cuts at: 2016 2467 2499 2797 2829 3135
Size: 451 32 298 32 306
Fragments arranged by size:
849 554 451 416 306 298 69 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 831 1088 3135
Size: 831 257 2047
Fragments arranged by size:
2047 831 257
HpaI GTT'AAC
Cuts at: 0 831 3135
Size: 831 2304
MfeI C'AATT_G
Cuts at: 0 544 3135
Size: 544 2591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3067 3135
Size: 3067 68
MscI TGG'CCA
Cuts at: 0 480 3135
Size: 480 2655
MslI CAynn'nnrTG
Cuts at: 0 640 1402 1567 2545 2833 3135
Size: 640 762 165 978 288 302
Fragments arranged by size:
978 762 640 302 288 165
MspA1I CmG'CkG
Cuts at: 0 870 2973 3091 3135
Size: 870 2103 118 44
Fragments arranged by size:
2103 870 118 44
NaeI GCC'GGC
Cuts at: 0 33 3135
Size: 33 3102
NcoI C'CATG_G
Cuts at: 0 998 1562 3135
Size: 998 564 1573
Fragments arranged by size:
1573 998 564
NdeI CA'TA_TG
Cuts at: 0 1403 3135
Size: 1403 1732
NgoMIV G'CCGG_C
Cuts at: 0 31 3135
Size: 31 3104
NheI G'CTAG_C
Cuts at: 0 816 3135
Size: 816 2319
NsiI A_TGCA'T
Cuts at: 0 380 3135
Size: 380 2755
NspI r_CATG'y
Cuts at: 0 512 1194 1332 1762 2215 2386 3135
Size: 512 682 138 430 453 171 749
Fragments arranged by size:
749 682 512 453 430 171 138
PciI A'CATG_T
Cuts at: 0 508 1190 3135
Size: 508 682 1945
Fragments arranged by size:
1945 682 508
PflMI CCAn_nnn'nTGG
Cuts at: 0 458 3135
Size: 458 2677
PfoI T'CCnGG_A
Cuts at: 0 788 1322 3135
Size: 788 534 1813
Fragments arranged by size:
1813 788 534
PpuMI rG'GwC_Cy
Cuts at: 0 105 3135
Size: 105 3030
PspOMI G'GGCC_C
Cuts at: 0 2707 3135
Size: 2707 428
PspXI vC'TCGA_Gb
Cuts at: 0 54 3135
Size: 54 3081
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1649 1681 3135
Size: 1649 32 1454
Fragments arranged by size:
1649 1454 32
PstI C_TGCA'G
Cuts at: 0 17 396 711 1268 2972 3018 3135
Size: 17 379 315 557 1704 46 117
Fragments arranged by size:
1704 557 379 315 117 46 17
PvuII CAG'CTG
Cuts at: 0 870 2973 3091 3135
Size: 870 2103 118 44
Fragments arranged by size:
2103 870 118 44
SapI GCTCTTCn'nnn_
Cuts at: 0 1485 2084 2269 3135
Size: 1485 599 185 866
Fragments arranged by size:
1485 866 599 185
ScaI AGT'ACT
Cuts at: 0 1695 3135
Size: 1695 1440
SexAI A'CCwGG_T
Cuts at: 0 2663 3135
Size: 2663 472
SfcI C'TryA_G
Cuts at: 0 13 215 392 707 1264 2968 3014 3135
Size: 13 202 177 315 557 1704 46 121
Fragments arranged by size:
1704 557 315 202 177 121 46 13
SmlI C'TyrA_G
Cuts at: 0 54 1101 1840 2029 2507 3135
Size: 54 1047 739 189 478 628
Fragments arranged by size:
1047 739 628 478 189 54
SphI G_CATG'C
Cuts at: 0 1762 2215 3135
Size: 1762 453 920
Fragments arranged by size:
1762 920 453
StuI AGG'CCT
Cuts at: 0 1907 2618 3135
Size: 1907 711 517
Fragments arranged by size:
1907 711 517
StyI C'CwwG_G
Cuts at: 0 998 1125 1454 1562 2891 2943 2979 3135
Size: 998 127 329 108 1329 52 36 156
Fragments arranged by size:
1329 998 329 156 127 108 52 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1544 2430 3135
Size: 1544 886 705
Fragments arranged by size:
1544 886 705
TatI w'GTAC_w
Cuts at: 0 594 1193 1357 1462 1468 1693 1718 1844
Size: 594 599 164 105 6 225 25 126
Cuts at: 1844 1937 2555 3135
Size: 93 618 580
Fragments arranged by size:
618 599 594 580 225 164 126 105
93 25 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 770 802 3135
Size: 770 32 2333
Fragments arranged by size:
2333 770 32
XbaI T'CTAG_A
Cuts at: 0 3003 3135
Size: 3003 132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2898 3135
Size: 2898 237
XhoI C'TCGA_G
Cuts at: 0 54 3135
Size: 54 3081
XmnI GAAnn'nnTTC
Cuts at: 0 899 3135
Size: 899 2236
Enzymes that do cut:
AccI AcuI AflIII AlfI AloI ApaI ApaLI ApoI
AseI AvaI AvrII BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstBI BstYI BtgI BtsI DraI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I HincII HpaI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PpuMI PspOMI PspXI PsrI PstI
PvuII SapI ScaI SexAI SfcI SmlI SphI StuI
StyI TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlwNI AscI AsiSI BaeI BamHI BbeI BbvCI
BlpI BmgBI BplI BsaAI BsaHI BsaWI BsiWI BsmBI
BspEI BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DrdI EciI EcoICRI EcoRV FseI FspI
FspAI HaeII HindIII KasI KpnI MluI NarI NotI
NruI PacI PasI PmeI PmlI PpiI PshAI PsiI
PvuI RsrII SacI SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XmaI ZraI