Restriction map for
pFN21AE6893
(Linear) (Six-base) MAPSORT of: KIEE6893.seq Check: 564 from: 1 to: 1095
KIEE6893.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AccI GT'mk_AC
Cuts at: 0 248 968 1095
Size: 248 720 127
Fragments arranged by size:
720 248 127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1027 1095
Size: 505 522 68
Fragments arranged by size:
522 505 68
AfeI AGC'GCT
Cuts at: 0 691 1095
Size: 691 404
AhdI GACnn_n'nnGTC
Cuts at: 0 447 1095
Size: 447 648
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 1095
Size: 397 698
AlwNI CAG_nnn'CTG
Cuts at: 0 997 1095
Size: 997 98
ApaI G_GGCC'C
Cuts at: 0 190 1095
Size: 190 905
AseI AT'TA_AT
Cuts at: 0 1055 1095
Size: 1055 40
AvaI C'yCGr_G
Cuts at: 0 1039 1095
Size: 1039 56
AvrII C'CTAG_G
Cuts at: 0 105 1095
Size: 105 990
BanI G'GyrC_C
Cuts at: 0 235 922 1095
Size: 235 687 173
Fragments arranged by size:
687 235 173
BanII G_rGCy'C
Cuts at: 0 190 1095
Size: 190 905
BbvCI CC'TCA_GC
Cuts at: 0 686 1095
Size: 686 409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 624 658 1095
Size: 624 34 437
Fragments arranged by size:
624 437 34
BfrBI ATG'CAT
Cuts at: 0 348 1095
Size: 348 747
BglI GCCn_nnn'nGGC
Cuts at: 0 358 949 1095
Size: 358 591 146
Fragments arranged by size:
591 358 146
BglII A'GATC_T
Cuts at: 0 1010 1095
Size: 1010 85
BlpI GC'TnA_GC
Cuts at: 0 1088 1095
Size: 1088 7
Bme1580I G_kGCm'C
Cuts at: 0 190 753 1095
Size: 190 563 342
Fragments arranged by size:
563 342 190
BmrI ACTGGGnnnn_n'
Cuts at: 0 145 1095
Size: 145 950
Bpu10I CC'TnA_GC
Cuts at: 0 686 1095
Size: 686 409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 1095
Size: 489 606
BsaAI yAC'GTr
Cuts at: 0 654 1095
Size: 654 441
BsaHI Gr'CG_yC
Cuts at: 0 647 1095
Size: 647 448
BsaWI w'CCGG_w
Cuts at: 0 8 1095
Size: 8 1087
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3 33 950 980 1095
Size: 3 30 917 30 115
Fragments arranged by size:
917 115 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 189 469 1095
Size: 189 280 626
Fragments arranged by size:
626 280 189
BseYI C'CCAG_C
Cuts at: 0 212 308 594 1095
Size: 212 96 286 501
Fragments arranged by size:
501 286 212 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 1095
Size: 544 551
BsiEI CG_ry'CG
Cuts at: 0 321 1095
Size: 321 774
Bsp1286I G_dGCh'C
Cuts at: 0 190 753 1095
Size: 190 563 342
Fragments arranged by size:
563 342 190
BspEI T'CCGG_A
Cuts at: 0 8 1095
Size: 8 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 666 1095
Size: 666 429
BsrFI r'CCGG_y
Cuts at: 0 260 1095
Size: 260 835
BstAPI GCAn_nnn'nTGC
Cuts at: 0 591 681 1095
Size: 591 90 414
Fragments arranged by size:
591 414 90
BstEII G'GTnAC_C
Cuts at: 0 777 1095
Size: 777 318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 1095
Size: 297 798
BstYI r'GATC_y
Cuts at: 0 474 539 1010 1095
Size: 474 65 471 85
Fragments arranged by size:
474 471 85 65
BtgI C'CryG_G
Cuts at: 0 418 660 734 868 1095
Size: 418 242 74 134 227
Fragments arranged by size:
418 242 227 134 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 196 845 863 1095
Size: 196 649 18 232
Fragments arranged by size:
649 232 196 18
DraIII CAC_nnn'GTG
Cuts at: 0 419 896 1095
Size: 419 477 199
Fragments arranged by size:
477 419 199
EaeI y'GGCC_r
Cuts at: 0 155 258 318 974 1095
Size: 155 103 60 656 121
Fragments arranged by size:
656 155 121 103 60
EagI C'GGCC_G
Cuts at: 0 318 1095
Size: 318 777
EarI CTCTTCn'nnn_
Cuts at: 0 207 572 1095
Size: 207 365 523
Fragments arranged by size:
523 365 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 595 971 1095
Size: 595 376 124
Fragments arranged by size:
595 376 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1027 1095
Size: 505 522 68
Fragments arranged by size:
522 505 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 727 1095
Size: 727 368
EcoO109I rG'GnC_Cy
Cuts at: 0 187 1095
Size: 187 908
HaeII r_GCGC'y
Cuts at: 0 693 719 1095
Size: 693 26 376
Fragments arranged by size:
693 376 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 108 140 433 465 950 982
Size: 3 32 73 32 293 32 485 32
Cuts at: 982 1095
Size: 113
Fragments arranged by size:
485 293 113 73 32 32 32 32
3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1095
Size: 431 664
MscI TGG'CCA
Cuts at: 0 157 976 1095
Size: 157 819 119
Fragments arranged by size:
819 157 119
MslI CAynn'nnrTG
Cuts at: 0 910 1095
Size: 910 185
NcoI C'CATG_G
Cuts at: 0 734 1095
Size: 734 361
NsiI A_TGCA'T
Cuts at: 0 350 1095
Size: 350 745
NspI r_CATG'y
Cuts at: 0 352 1095
Size: 352 743
PasI CC'CwG_GG
Cuts at: 0 825 1095
Size: 825 270
PflMI CCAn_nnn'nTGG
Cuts at: 0 740 1095
Size: 740 355
PfoI T'CCnGG_A
Cuts at: 0 530 1095
Size: 530 565
PmlI CAC'GTG
Cuts at: 0 654 1095
Size: 654 441
PspOMI G'GGCC_C
Cuts at: 0 186 1095
Size: 186 909
PstI C_TGCA'G
Cuts at: 0 434 677 1095
Size: 434 243 418
Fragments arranged by size:
434 418 243
SfcI C'TryA_G
Cuts at: 0 430 673 1095
Size: 430 243 422
Fragments arranged by size:
430 422 243
SmlI C'TyrA_G
Cuts at: 0 468 1095
Size: 468 627
SphI G_CATG'C
Cuts at: 0 352 1095
Size: 352 743
StuI AGG'CCT
Cuts at: 0 110 1095
Size: 110 985
StyI C'CwwG_G
Cuts at: 0 105 734 1095
Size: 105 629 361
Fragments arranged by size:
629 361 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1035 1095
Size: 1035 60
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 1095
Size: 208 32 855
Fragments arranged by size:
855 208 32
Tth111I GACn'n_nGTC
Cuts at: 0 964 1095
Size: 964 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 658 789 1095
Size: 658 131 306
Fragments arranged by size:
658 306 131
Enzymes that do cut:
AccI AcuI AfeI AhdI AlfI AlwNI ApaI AseI
AvaI AvrII BanI BanII BbvCI BcgI BfrBI BglI
BglII BlpI Bme1580I BmrI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspEI
BspMI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI
DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I MmeI MscI MslI NcoI NsiI NspI
PasI PflMI PfoI PmlI PspOMI PstI SfcI SmlI
SphI StuI StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AleI
AloI ApaLI ApoI AscI AsiSI BaeI BamHI BbeI
BbsI BciVI BclI BmgBI BmtI BplI BpmI BsaI
BsaBI BsiHKAI BsiWI BsmI BsmBI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DrdI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
XbaI XhoI XmaI XmnI ZraI