Restriction map for
pFN21AE6819
(Linear) (Six-base) MAPSORT of: KIEE6819.seq Check: 9547 from: 1 to: 1026
KIEE6819.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 199 1026
Size: 199 827
AatII G_ACGT'C
Cuts at: 0 73 1026
Size: 73 953
AccI GT'mk_AC
Cuts at: 0 377 1026
Size: 377 649
AclI AA'CG_TT
Cuts at: 0 222 1026
Size: 222 804
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1026
Size: 366 660
AhdI GACnn_n'nnGTC
Cuts at: 0 74 1026
Size: 74 952
AlwNI CAG_nnn'CTG
Cuts at: 0 12 490 552 807 1026
Size: 12 478 62 255 219
Fragments arranged by size:
478 255 219 62 12
ApaLI G'TGCA_C
Cuts at: 0 159 1026
Size: 159 867
ApoI r'AATT_y
Cuts at: 0 329 1026
Size: 329 697
BanI G'GyrC_C
Cuts at: 0 22 318 1026
Size: 22 296 708
Fragments arranged by size:
708 296 22
BbeI G_GCGC'C
Cuts at: 0 26 1026
Size: 26 1000
BbsI GAAGACnn'nnnn_
Cuts at: 0 789 1026
Size: 789 237
BbvCI CC'TCA_GC
Cuts at: 0 58 833 1026
Size: 58 775 193
Fragments arranged by size:
775 193 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 648 682 1026
Size: 648 34 344
Fragments arranged by size:
648 344 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 441 1026
Size: 441 585
Bme1580I G_kGCm'C
Cuts at: 0 163 1026
Size: 163 863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 1026
Size: 574 452
Bpu10I CC'TnA_GC
Cuts at: 0 58 452 833 1026
Size: 58 394 381 193
Fragments arranged by size:
394 381 193 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1026
Size: 308 718
BsaHI Gr'CG_yC
Cuts at: 0 23 70 1026
Size: 23 47 956
Fragments arranged by size:
956 47 23
BsaWI w'CCGG_w
Cuts at: 0 73 1026
Size: 73 953
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 180 210 1026
Size: 180 30 816
Fragments arranged by size:
816 180 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 188 561 1026
Size: 75 113 373 465
Fragments arranged by size:
465 373 113 75
BseYI C'CCAG_C
Cuts at: 0 631 1026
Size: 631 395
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 389 1026
Size: 177 212 637
Fragments arranged by size:
637 212 177
BsiHKAI G_wGCw'C
Cuts at: 0 163 180 1026
Size: 163 17 846
Fragments arranged by size:
846 163 17
BsmI GAATG_Cn'
Cuts at: 0 541 1026
Size: 541 485
BsmBI CGTCTCn'nnnn_
Cuts at: 0 675 1026
Size: 675 351
Bsp1286I G_dGCh'C
Cuts at: 0 163 180 1026
Size: 163 17 846
Fragments arranged by size:
846 163 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 199 1026
Size: 199 827
BssSI C'ACGA_G
Cuts at: 0 724 1026
Size: 724 302
DraI TTT'AAA
Cuts at: 0 1013 1026
Size: 1013 13
EaeI y'GGCC_r
Cuts at: 0 903 1026
Size: 903 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 574 1026
Size: 366 208 452
Fragments arranged by size:
452 366 208
EcoNI CCTnn'n_nnAGG
Cuts at: 0 278 1026
Size: 278 748
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 1026
Size: 369 657
HaeII r_GCGC'y
Cuts at: 0 26 1026
Size: 26 1000
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 903 935 1026
Size: 60 32 811 32 91
Fragments arranged by size:
811 91 60 32 32
HindIII A'AGCT_T
Cuts at: 0 39 1007 1026
Size: 39 968 19
Fragments arranged by size:
968 39 19
KasI G'GCGC_C
Cuts at: 0 22 1026
Size: 22 1004
MfeI C'AATT_G
Cuts at: 0 839 1026
Size: 839 187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 989 1026
Size: 989 37
MscI TGG'CCA
Cuts at: 0 905 1026
Size: 905 121
MspA1I CmG'CkG
Cuts at: 0 166 807 1026
Size: 166 641 219
Fragments arranged by size:
641 219 166
NarI GG'CG_CC
Cuts at: 0 23 1026
Size: 23 1003
NspI r_CATG'y
Cuts at: 0 941 1026
Size: 941 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 807 1026
Size: 807 219
PstI C_TGCA'G
Cuts at: 0 142 503 1026
Size: 142 361 523
Fragments arranged by size:
523 361 142
PvuII CAG'CTG
Cuts at: 0 166 807 1026
Size: 166 641 219
Fragments arranged by size:
641 219 166
SfcI C'TryA_G
Cuts at: 0 131 138 499 1026
Size: 131 7 361 527
Fragments arranged by size:
527 361 131 7
SfoI GGC'GCC
Cuts at: 0 24 1026
Size: 24 1002
SmlI C'TyrA_G
Cuts at: 0 323 1026
Size: 323 703
StuI AGG'CCT
Cuts at: 0 261 1026
Size: 261 765
StyI C'CwwG_G
Cuts at: 0 625 1026
Size: 625 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 1026
Size: 163 863
TatI w'GTAC_w
Cuts at: 0 182 638 1026
Size: 182 456 388
Fragments arranged by size:
456 388 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 180 212 636 668 1026
Size: 180 32 424 32 358
Fragments arranged by size:
424 358 180 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 333 1026
Size: 333 693
ZraI GAC'GTC
Cuts at: 0 71 1026
Size: 71 955
Enzymes that do cut:
AarI AatII AccI AclI AcuI AhdI AlwNI ApaLI
ApoI BanI BbeI BbsI BbvCI BcgI BciVI Bme1580I
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BssSI DraI
EaeI Eco57MI EcoNI FalI HaeII Hin4I HindIII KasI
MfeI MmeI MscI MspA1I NarI NspI PflMI PstI
PvuII SfcI SfoI SmlI StuI StyI TaqII TatI
TstI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AgeI AleI AlfI AloI
ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanII BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI BsaI BsaAI BsaBI BsiEI BsiWI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EarI EciI EcoICRI
EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HpaI
KpnI MluI MslI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmaI