Restriction map for
pFN21AE6794
(Linear) (Six-base) MAPSORT of: KIEE6794.seq Check: 924 from: 1 to: 3396
KIEE6794.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AccI GT'mk_AC
Cuts at: 0 157 254 2781 2975 3396
Size: 157 97 2527 194 421
Fragments arranged by size:
2527 421 194 157 97
AclI AA'CG_TT
Cuts at: 0 849 3396
Size: 849 2547
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3055 3329 3396
Size: 3055 274 67
Fragments arranged by size:
3055 274 67
AhdI GACnn_n'nnGTC
Cuts at: 0 3346 3396
Size: 3346 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2200 2382 2872 3396
Size: 2200 182 490 524
Fragments arranged by size:
2200 524 490 182
AlwNI CAG_nnn'CTG
Cuts at: 0 31 2712 3396
Size: 31 2681 684
Fragments arranged by size:
2681 684 31
ApaI G_GGCC'C
Cuts at: 0 2872 3396
Size: 2872 524
ApoI r'AATT_y
Cuts at: 0 168 213 667 1108 1267 1929 2289 2418
Size: 168 45 454 441 159 662 360 129
Cuts at: 2418 2840 3396
Size: 422 556
Fragments arranged by size:
662 556 454 441 422 360 168 159
129 45
AseI AT'TA_AT
Cuts at: 0 872 1163 2254 3396
Size: 872 291 1091 1142
Fragments arranged by size:
1142 1091 872 291
BanI G'GyrC_C
Cuts at: 0 2379 2458 3396
Size: 2379 79 938
Fragments arranged by size:
2379 938 79
BanII G_rGCy'C
Cuts at: 0 1120 1176 2099 2872 3396
Size: 1120 56 923 773 524
Fragments arranged by size:
1120 923 773 524 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 1669 1961 3185 3396
Size: 1669 292 1224 211
Fragments arranged by size:
1669 1224 292 211
BciVI GTATCCnnnnn_n'
Cuts at: 0 3042 3210 3396
Size: 3042 168 186
Fragments arranged by size:
3042 186 168
Bme1580I G_kGCm'C
Cuts at: 0 2872 3396
Size: 2872 524
BmgBI CAC'GTC
Cuts at: 0 3065 3396
Size: 3065 331
BmrI ACTGGGnnnn_n'
Cuts at: 0 265 2039 3396
Size: 265 1774 1357
Fragments arranged by size:
1774 1357 265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 62 1805 3396
Size: 8 54 1743 1591
Fragments arranged by size:
1743 1591 54 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1298 1652 3136 3396
Size: 724 574 354 1484 260
Fragments arranged by size:
1484 724 574 354 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 1000 1975 3396
Size: 1000 975 1421
Fragments arranged by size:
1421 1000 975
BsaAI yAC'GTr
Cuts at: 0 580 1399 3396
Size: 580 819 1997
Fragments arranged by size:
1997 819 580
BsaBI GATnn'nnATC
Cuts at: 0 1376 1784 3396
Size: 1376 408 1612
Fragments arranged by size:
1612 1376 408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 776 806 1163 1193 3396
Size: 776 30 357 30 2203
Fragments arranged by size:
2203 776 357 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1476 3396
Size: 1476 1920
BsiHKAI G_wGCw'C
Cuts at: 0 2027 2099 2366 3396
Size: 2027 72 267 1030
Fragments arranged by size:
2027 1030 267 72
BsmI GAATG_Cn'
Cuts at: 0 1282 3396
Size: 1282 2114
Bsp1286I G_dGCh'C
Cuts at: 0 1120 1176 2027 2099 2366 2872 3396
Size: 1120 56 851 72 267 506 524
Fragments arranged by size:
1120 851 524 506 267 72 56
BspHI T'CATG_A
Cuts at: 0 1734 3396
Size: 1734 1662
BsrDI GCAATG_nn'
Cuts at: 0 113 587 1781 2856 3396
Size: 113 474 1194 1075 540
Fragments arranged by size:
1194 1075 540 474 113
BsrGI T'GTAC_A
Cuts at: 0 240 598 2628 2738 3396
Size: 240 358 2030 110 658
Fragments arranged by size:
2030 658 358 240 110
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 3210 3396
Size: 723 2487 186
Fragments arranged by size:
2487 723 186
BstYI r'GATC_y
Cuts at: 0 2820 3396
Size: 2820 576
BstZ17I GTA'TAC
Cuts at: 0 158 2976 3396
Size: 158 2818 420
Fragments arranged by size:
2818 420 158
BtsI GCAGTG_nn'
Cuts at: 0 1943 2366 3396
Size: 1943 423 1030
Fragments arranged by size:
1943 1030 423
ClaI AT'CG_AT
Cuts at: 0 614 2148 3396
Size: 614 1534 1248
Fragments arranged by size:
1534 1248 614
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1932 1967 2657 2692 3396
Size: 1932 35 690 35 704
Fragments arranged by size:
1932 704 690 35 35
DraI TTT'AAA
Cuts at: 0 1022 2220 2423 3396
Size: 1022 1198 203 973
Fragments arranged by size:
1198 1022 973 203
DraIII CAC_nnn'GTG
Cuts at: 0 1798 3081 3396
Size: 1798 1283 315
Fragments arranged by size:
1798 1283 315
DrdI GACnn_nn'nnGTC
Cuts at: 0 364 3396
Size: 364 3032
EaeI y'GGCC_r
Cuts at: 0 1290 3396
Size: 1290 2106
EarI CTCTTCn'nnn_
Cuts at: 0 41 1379 1494 3362 3396
Size: 41 1338 115 1868 34
Fragments arranged by size:
1868 1338 115 41 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3324 3396
Size: 3324 72
EcoICRI GAG'CTC
Cuts at: 0 2097 3396
Size: 2097 1299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 62 1805 3055 3329 3396
Size: 8 54 1743 1250 274 67
Fragments arranged by size:
1743 1250 274 67 54 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 3396
Size: 241 3155
EcoO109I rG'GnC_Cy
Cuts at: 0 727 2869 3396
Size: 727 2142 527
Fragments arranged by size:
2142 727 527
EcoRI G'AATT_C
Cuts at: 0 1267 3396
Size: 1267 2129
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3060 3396
Size: 3060 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 535 567 1027 1059 1315 1347 2157 2189
Size: 535 32 460 32 256 32 810 32
Cuts at: 2189 2223 2255 2805 2837 3316 3332 3348 3364
Size: 34 32 550 32 479 16 16 16
Cuts at: 3364 3396
Size: 32
Fragments arranged by size:
810 550 535 479 460 256 34 32
32 32 32 32 32 32 16 16
16
HincII GTy'rAC
Cuts at: 0 369 1182 1748 3396
Size: 369 813 566 1648
Fragments arranged by size:
1648 813 566 369
HindIII A'AGCT_T
Cuts at: 0 107 2214 3037 3396
Size: 107 2107 823 359
Fragments arranged by size:
2107 823 359 107
HpaI GTT'AAC
Cuts at: 0 1182 1748 3396
Size: 1182 566 1648
Fragments arranged by size:
1648 1182 566
MfeI C'AATT_G
Cuts at: 0 555 632 3396
Size: 555 77 2764
Fragments arranged by size:
2764 555 77
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2353 3396
Size: 2353 1043
MscI TGG'CCA
Cuts at: 0 1292 3396
Size: 1292 2104
MslI CAynn'nnrTG
Cuts at: 0 802 3396
Size: 802 2594
MspA1I CmG'CkG
Cuts at: 0 1468 3396
Size: 1468 1928
NspI r_CATG'y
Cuts at: 0 2002 2063 3396
Size: 2002 61 1333
Fragments arranged by size:
2002 1333 61
PpuMI rG'GwC_Cy
Cuts at: 0 727 3396
Size: 727 2669
PsiI TTA'TAA
Cuts at: 0 304 3396
Size: 304 3092
PspOMI G'GGCC_C
Cuts at: 0 2868 3396
Size: 2868 528
PstI C_TGCA'G
Cuts at: 0 921 1950 1980 2361 3396
Size: 921 1029 30 381 1035
Fragments arranged by size:
1035 1029 921 381 30
PvuII CAG'CTG
Cuts at: 0 1468 3396
Size: 1468 1928
SacI G_AGCT'C
Cuts at: 0 2099 3396
Size: 2099 1297
SapI GCTCTTCn'nnn_
Cuts at: 0 1379 3396
Size: 1379 2017
ScaI AGT'ACT
Cuts at: 0 2208 2635 3396
Size: 2208 427 761
Fragments arranged by size:
2208 761 427
SfcI C'TryA_G
Cuts at: 0 530 690 917 1421 1457 1946 1976 2357
Size: 530 160 227 504 36 489 30 381
Cuts at: 2357 2775 2860 3309 3396
Size: 418 85 449 87
Fragments arranged by size:
530 504 489 449 418 381 227 160
87 85 36 30
SmlI C'TyrA_G
Cuts at: 0 703 1313 1667 3151 3396
Size: 703 610 354 1484 245
Fragments arranged by size:
1484 703 610 354 245
SphI G_CATG'C
Cuts at: 0 2002 3396
Size: 2002 1394
SspI AAT'ATT
Cuts at: 0 3230 3396
Size: 3230 166
StuI AGG'CCT
Cuts at: 0 2009 3396
Size: 2009 1387
StyI C'CwwG_G
Cuts at: 0 831 973 3044 3396
Size: 831 142 2071 352
Fragments arranged by size:
2071 831 352 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2010 3396
Size: 2010 1386
TatI w'GTAC_w
Cuts at: 0 240 453 598 2206 2353 2628 2633 2738
Size: 240 213 145 1608 147 275 5 105
Cuts at: 2738 3396
Size: 658
Fragments arranged by size:
1608 658 275 240 213 147 145 105
5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1782 1814 3396
Size: 1782 32 1582
Fragments arranged by size:
1782 1582 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2689 2862 3396
Size: 2689 173 534
Fragments arranged by size:
2689 534 173
XmnI GAAnn'nnTTC
Cuts at: 0 470 1610 3396
Size: 470 1140 1786
Fragments arranged by size:
1786 1140 470
Enzymes that do cut:
AccI AclI AcuI AhdI AlfI AlwNI ApaI ApoI
AseI BanI BanII BbsI BciVI Bme1580I BmgBI BmrI
BpmI BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrGI BstXI BstYI BstZ17I
BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NspI PpuMI PsiI PspOMI PstI PvuII SacI SapI
ScaI SfcI SmlI SphI SspI StuI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AleI
AloI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmtI BplI Bpu10I BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII Bsu36I BtgI BtgZI EagI EcoRV
FseI FspI FspAI HaeII KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI