Restriction map for
pFN21AE6792
(Linear) (Six-base) MAPSORT of: KIEE6792.seq Check: 1679 from: 1 to: 2370
KIEE6792.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 505 830 2279 2370
Size: 122 383 325 1449 91
Fragments arranged by size:
1449 383 325 122 91
AflII C'TTAA_G
Cuts at: 0 67 519 1195 2370
Size: 67 452 676 1175
Fragments arranged by size:
1175 676 452 67
AflIII A'CryG_T
Cuts at: 0 468 786 1159 1364 2370
Size: 468 318 373 205 1006
Fragments arranged by size:
1006 468 373 318 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1322 1422 2370
Size: 1322 100 948
Fragments arranged by size:
1322 948 100
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1407 1439 2370
Size: 1407 32 931
Fragments arranged by size:
1407 931 32
ApoI r'AATT_y
Cuts at: 0 1416 1619 1626 1650 2370
Size: 1416 203 7 24 720
Fragments arranged by size:
1416 720 203 24 7
AseI AT'TA_AT
Cuts at: 0 2095 2370
Size: 2095 275
AvaI C'yCGr_G
Cuts at: 0 1919 2370
Size: 1919 451
AvrII C'CTAG_G
Cuts at: 0 1806 2370
Size: 1806 564
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1353 1386 2370
Size: 1353 33 984
Fragments arranged by size:
1353 984 33
BamHI G'GATC_C
Cuts at: 0 589 2370
Size: 589 1781
BanI G'GyrC_C
Cuts at: 0 1306 1825 1968 2370
Size: 1306 519 143 402
Fragments arranged by size:
1306 519 402 143
BanII G_rGCy'C
Cuts at: 0 343 443 1093 2370
Size: 343 100 650 1277
Fragments arranged by size:
1277 650 343 100
BciVI GTATCCnnnnn_n'
Cuts at: 0 734 1354 2370
Size: 734 620 1016
Fragments arranged by size:
1016 734 620
BclI T'GATC_A
Cuts at: 0 633 1590 2370
Size: 633 957 780
Fragments arranged by size:
957 780 633
BfrBI ATG'CAT
Cuts at: 0 386 2370
Size: 386 1984
BglI GCCn_nnn'nGGC
Cuts at: 0 1549 2370
Size: 1549 821
BglII A'GATC_T
Cuts at: 0 14 2370
Size: 14 2356
BlpI GC'TnA_GC
Cuts at: 0 145 2370
Size: 145 2225
Bme1580I G_kGCm'C
Cuts at: 0 879 1765 1971 2370
Size: 879 886 206 399
Fragments arranged by size:
886 879 399 206
BmgBI CAC'GTC
Cuts at: 0 219 2370
Size: 219 2151
BmrI ACTGGGnnnn_n'
Cuts at: 0 452 710 1112 2370
Size: 452 258 402 1258
Fragments arranged by size:
1258 452 402 258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 2370
Size: 132 2238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 2006 2370
Size: 319 1687 364
Fragments arranged by size:
1687 364 319
Bpu10I CC'TnA_GC
Cuts at: 0 574 1092 2370
Size: 574 518 1278
Fragments arranged by size:
1278 574 518
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 656 2370
Size: 430 226 1714
Fragments arranged by size:
1714 430 226
BsaI GGTCTCn'nnnn_
Cuts at: 0 400 2370
Size: 400 1970
BsaAI yAC'GTr
Cuts at: 0 1365 2370
Size: 1365 1005
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 325 355 2370
Size: 325 30 2015
Fragments arranged by size:
2015 325 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1270 2370
Size: 1270 1100
BseYI C'CCAG_C
Cuts at: 0 1017 1757 2370
Size: 1017 740 613
Fragments arranged by size:
1017 740 613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 184 1518 2370
Size: 31 153 1334 852
Fragments arranged by size:
1334 852 153 31
Bsp1286I G_dGCh'C
Cuts at: 0 343 443 879 1093 1765 1971 2370
Size: 343 100 436 214 672 206 399
Fragments arranged by size:
672 436 399 343 214 206 100
BspHI T'CATG_A
Cuts at: 0 1011 1335 2370
Size: 1011 324 1035
Fragments arranged by size:
1035 1011 324
BsrGI T'GTAC_A
Cuts at: 0 2217 2370
Size: 2217 153
BstBI TT'CG_AA
Cuts at: 0 1623 2370
Size: 1623 747
BstYI r'GATC_y
Cuts at: 0 14 589 1501 2370
Size: 14 575 912 869
Fragments arranged by size:
912 869 575 14
BtgI C'CryG_G
Cuts at: 0 1544 1790 2370
Size: 1544 246 580
Fragments arranged by size:
1544 580 246
BtsI GCAGTG_nn'
Cuts at: 0 1522 1950 2370
Size: 1522 428 420
Fragments arranged by size:
1522 428 420
DraI TTT'AAA
Cuts at: 0 1137 1632 1857 2370
Size: 1137 495 225 513
Fragments arranged by size:
1137 513 495 225
DrdI GACnn_nn'nnGTC
Cuts at: 0 1331 2370
Size: 1331 1039
EarI CTCTTCn'nnn_
Cuts at: 0 1152 2370
Size: 1152 1218
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 319 505 830 2006 2279 2370
Size: 122 197 186 325 1176 273 91
Fragments arranged by size:
1176 325 273 197 186 122 91
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1805 2370
Size: 1805 565
EcoRI G'AATT_C
Cuts at: 0 1416 1619 2370
Size: 1416 203 751
Fragments arranged by size:
1416 751 203
EcoRV GAT'ATC
Cuts at: 0 796 2370
Size: 796 1574
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 165 2370
Size: 165 2205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1813 1845 2370
Size: 1813 32 525
Fragments arranged by size:
1813 525 32
HincII GTy'rAC
Cuts at: 0 1578 2370
Size: 1578 792
HpaI GTT'AAC
Cuts at: 0 1578 2370
Size: 1578 792
MfeI C'AATT_G
Cuts at: 0 1113 2370
Size: 1113 1257
MluI A'CGCG_T
Cuts at: 0 786 2370
Size: 786 1584
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 771 2370
Size: 79 692 1599
Fragments arranged by size:
1599 692 79
MslI CAynn'nnrTG
Cuts at: 0 385 2074 2370
Size: 385 1689 296
Fragments arranged by size:
1689 385 296
NcoI C'CATG_G
Cuts at: 0 1544 1790 2370
Size: 1544 246 580
Fragments arranged by size:
1544 580 246
NsiI A_TGCA'T
Cuts at: 0 388 2370
Size: 388 1982
NspI r_CATG'y
Cuts at: 0 386 472 1163 1959 2370
Size: 386 86 691 796 411
Fragments arranged by size:
796 691 411 386 86
PciI A'CATG_T
Cuts at: 0 468 1159 2370
Size: 468 691 1211
Fragments arranged by size:
1211 691 468
PfoI T'CCnGG_A
Cuts at: 0 585 2022 2370
Size: 585 1437 348
Fragments arranged by size:
1437 585 348
PsiI TTA'TAA
Cuts at: 0 1445 2370
Size: 1445 925
PstI C_TGCA'G
Cuts at: 0 200 1889 2370
Size: 200 1689 481
Fragments arranged by size:
1689 481 200
ScaI AGT'ACT
Cuts at: 0 610 966 1405 2370
Size: 610 356 439 965
Fragments arranged by size:
965 610 439 356
SexAI A'CCwGG_T
Cuts at: 0 1000 1302 2370
Size: 1000 302 1068
Fragments arranged by size:
1068 1000 302
SfcI C'TryA_G
Cuts at: 0 196 1028 1314 1669 1885 2370
Size: 196 832 286 355 216 485
Fragments arranged by size:
832 485 355 286 216 196
SmaI CCC'GGG
Cuts at: 0 1921 2370
Size: 1921 449
SmlI C'TyrA_G
Cuts at: 0 67 445 519 671 1195 2370
Size: 67 378 74 152 524 1175
Fragments arranged by size:
1175 524 378 152 74 67
SpeI A'CTAG_T
Cuts at: 0 48 2370
Size: 48 2322
StuI AGG'CCT
Cuts at: 0 451 2370
Size: 451 1919
StyI C'CwwG_G
Cuts at: 0 1544 1790 1806 2370
Size: 1544 246 16 564
Fragments arranged by size:
1544 564 246 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 617 1952 2370
Size: 617 1335 418
Fragments arranged by size:
1335 617 418
TatI w'GTAC_w
Cuts at: 0 608 964 1403 2217 2370
Size: 608 356 439 814 153
Fragments arranged by size:
814 608 439 356 153
XbaI T'CTAG_A
Cuts at: 0 942 2370
Size: 942 1428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1528 1788 1957 2370
Size: 1528 260 169 413
Fragments arranged by size:
1528 413 260 169
XmaI C'CCGG_G
Cuts at: 0 1919 2370
Size: 1919 451
Enzymes that do cut:
AcuI AflII AflIII AlfI AloI ApoI AseI AvaI
AvrII BaeI BamHI BanI BanII BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI
Bsp1286I BspHI BsrGI BstBI BstYI BtgI BtsI DraI
DrdI EarI Eco57MI EcoNI EcoRI EcoRV FalI Hin4I
HincII HpaI MfeI MluI MmeI MslI NcoI NsiI
NspI PciI PfoI PsiI PstI ScaI SexAI SfcI
SmaI SmlI SpeI StuI StyI TaqII TatI XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlwNI ApaI ApaLI AscI AsiSI BbeI BbsI
BbvCI BcgI BmtI BsaBI BsaHI BsaWI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII EaeI EagI EciI EcoICRI EcoO109I
FseI FspI FspAI HaeII HindIII KasI KpnI MscI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SnaBI SphI SrfI SspI SwaI TstI Tth111I XhoI
XmnI ZraI