Restriction map for
pFN21AE6791
(Linear) (Six-base) MAPSORT of: KIEE6791.seq Check: 2110 from: 1 to: 2328
KIEE6791.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AccI GT'mk_AC
Cuts at: 0 1545 2328
Size: 1545 783
AclI AA'CG_TT
Cuts at: 0 857 2328
Size: 857 1471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 1390 2328
Size: 751 639 938
Fragments arranged by size:
938 751 639
AflIII A'CryG_T
Cuts at: 0 1669 2280 2328
Size: 1669 611 48
Fragments arranged by size:
1669 611 48
AleI CACnn'nnGTG
Cuts at: 0 2238 2328
Size: 2238 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1540 2328
Size: 1540 788
ApaLI G'TGCA_C
Cuts at: 0 172 2328
Size: 172 2156
ApoI r'AATT_y
Cuts at: 0 1273 1519 2328
Size: 1273 246 809
Fragments arranged by size:
1273 809 246
BanI G'GyrC_C
Cuts at: 0 2124 2328
Size: 2124 204
BanII G_rGCy'C
Cuts at: 0 167 2328
Size: 167 2161
BbsI GAAGACnn'nnnn_
Cuts at: 0 1142 2328
Size: 1142 1186
BclI T'GATC_A
Cuts at: 0 293 2328
Size: 293 2035
BfrBI ATG'CAT
Cuts at: 0 1248 2328
Size: 1248 1080
BglII A'GATC_T
Cuts at: 0 2003 2328
Size: 2003 325
Bme1580I G_kGCm'C
Cuts at: 0 176 1930 2127 2328
Size: 176 1754 197 201
Fragments arranged by size:
1754 201 197 176
BmgBI CAC'GTC
Cuts at: 0 1670 2328
Size: 1670 658
BmrI ACTGGGnnnn_n'
Cuts at: 0 246 1206 2328
Size: 246 960 1122
Fragments arranged by size:
1122 960 246
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1745 2328
Size: 1745 583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 430 1139 2328
Size: 251 179 709 1189
Fragments arranged by size:
1189 709 251 179
BsaI GGTCTCn'nnnn_
Cuts at: 0 953 2328
Size: 953 1375
BsaBI GATnn'nnATC
Cuts at: 0 2008 2328
Size: 2008 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1112 1142 1618 1648 2328
Size: 1112 30 476 30 680
Fragments arranged by size:
1112 680 476 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 89 2328
Size: 89 2239
BseYI C'CCAG_C
Cuts at: 0 1922 2328
Size: 1922 406
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 859 1642 1912 2328
Size: 386 473 783 270 416
Fragments arranged by size:
783 473 416 386 270
BsiHKAI G_wGCw'C
Cuts at: 0 176 2328
Size: 176 2152
BsmI GAATG_Cn'
Cuts at: 0 557 1653 1881 2328
Size: 557 1096 228 447
Fragments arranged by size:
1096 557 447 228
Bsp1286I G_dGCh'C
Cuts at: 0 167 176 1930 2127 2328
Size: 167 9 1754 197 201
Fragments arranged by size:
1754 201 197 167 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2074 2328
Size: 2074 254
BsrDI GCAATG_nn'
Cuts at: 0 1590 2328
Size: 1590 738
BssSI C'ACGA_G
Cuts at: 0 1174 2328
Size: 1174 1154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 441 1514 1723 2328
Size: 441 1073 209 605
Fragments arranged by size:
1073 605 441 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1180 2328
Size: 1180 1148
BstYI r'GATC_y
Cuts at: 0 24 32 1443 1631 2003 2328
Size: 24 8 1411 188 372 325
Fragments arranged by size:
1411 372 325 188 24 8
BtgI C'CryG_G
Cuts at: 0 483 2328
Size: 483 1845
BtsI GCAGTG_nn'
Cuts at: 0 95 161 1438 2106 2328
Size: 95 66 1277 668 222
Fragments arranged by size:
1277 668 222 95 66
DraIII CAC_nnn'GTG
Cuts at: 0 1214 2328
Size: 1214 1114
EaeI y'GGCC_r
Cuts at: 0 228 1707 2328
Size: 228 1479 621
Fragments arranged by size:
1479 621 228
EarI CTCTTCn'nnn_
Cuts at: 0 114 194 674 2328
Size: 114 80 480 1654
Fragments arranged by size:
1654 480 114 80
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 430 751 1139 1390 2328
Size: 251 179 321 388 251 938
Fragments arranged by size:
938 388 321 251 251 179
EcoNI CCTnn'n_nnAGG
Cuts at: 0 445 2328
Size: 445 1883
EcoO109I rG'GnC_Cy
Cuts at: 0 245 501 2328
Size: 245 256 1827
Fragments arranged by size:
1827 256 245
EcoRV GAT'ATC
Cuts at: 0 808 1824 2328
Size: 808 1016 504
Fragments arranged by size:
1016 808 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 353 385 1581 1613 1618 1650 2328
Size: 353 32 1196 32 5 32 678
Fragments arranged by size:
1196 678 353 32 32 32 5
HincII GTy'rAC
Cuts at: 0 674 2328
Size: 674 1654
HindIII A'AGCT_T
Cuts at: 0 1069 2328
Size: 1069 1259
HpaI GTT'AAC
Cuts at: 0 674 2328
Size: 674 1654
MfeI C'AATT_G
Cuts at: 0 709 2328
Size: 709 1619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1792 2328
Size: 1792 536
MscI TGG'CCA
Cuts at: 0 230 1709 2328
Size: 230 1479 619
Fragments arranged by size:
1479 619 230
MslI CAynn'nnrTG
Cuts at: 0 488 1907 2238 2328
Size: 488 1419 331 90
Fragments arranged by size:
1419 488 331 90
MspA1I CmG'CkG
Cuts at: 0 768 2328
Size: 768 1560
NcoI C'CATG_G
Cuts at: 0 483 2328
Size: 483 1845
NdeI CA'TA_TG
Cuts at: 0 1800 2328
Size: 1800 528
NsiI A_TGCA'T
Cuts at: 0 1250 2328
Size: 1250 1078
NspI r_CATG'y
Cuts at: 0 439 1252 2284 2328
Size: 439 813 1032 44
Fragments arranged by size:
1032 813 439 44
PciI A'CATG_T
Cuts at: 0 2280 2328
Size: 2280 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 1922 2328
Size: 1922 406
PfoI T'CCnGG_A
Cuts at: 0 321 446 464 2328
Size: 321 125 18 1864
Fragments arranged by size:
1864 321 125 18
PpuMI rG'GwC_Cy
Cuts at: 0 501 2328
Size: 501 1827
PsiI TTA'TAA
Cuts at: 0 1004 2328
Size: 1004 1324
PstI C_TGCA'G
Cuts at: 0 1288 2328
Size: 1288 1040
SanDI GG'GwC_CC
Cuts at: 0 501 2328
Size: 501 1827
ScaI AGT'ACT
Cuts at: 0 1311 1375 2328
Size: 1311 64 953
Fragments arranged by size:
1311 953 64
SfcI C'TryA_G
Cuts at: 0 336 556 1231 1254 1284 1886 2328
Size: 336 220 675 23 30 602 442
Fragments arranged by size:
675 602 442 336 220 30 23
SphI G_CATG'C
Cuts at: 0 439 1252 2328
Size: 439 813 1076
Fragments arranged by size:
1076 813 439
SspI AAT'ATT
Cuts at: 0 801 1300 2328
Size: 801 499 1028
Fragments arranged by size:
1028 801 499
StyI C'CwwG_G
Cuts at: 0 483 512 1758 2328
Size: 483 29 1246 570
Fragments arranged by size:
1246 570 483 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 1225 1762 1771 2328
Size: 148 1077 537 9 557
Fragments arranged by size:
1077 557 537 148 9
TatI w'GTAC_w
Cuts at: 0 1309 1373 1465 2328
Size: 1309 64 92 863
Fragments arranged by size:
1309 863 92 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2192 2224 2328
Size: 2192 32 104
Fragments arranged by size:
2192 104 32
XbaI T'CTAG_A
Cuts at: 0 117 2328
Size: 117 2211
XmnI GAAnn'nnTTC
Cuts at: 0 1651 2328
Size: 1651 677
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AlfI ApaLI ApoI
BanI BanII BbsI BclI BfrBI BglII Bme1580I BmgBI
BmrI BplI BpmI BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BstAPI
BstXI BstYI BtgI BtsI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I EcoRV Hin4I HincII HindIII HpaI MfeI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PciI PflMI PfoI PpuMI PsiI PstI SanDI ScaI
SfcI SphI SspI StyI TaqII TatI TstI XbaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BglI BlpI BmtI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoRI FalI FseI FspI FspAI HaeII
KasI KpnI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI StuI SwaI Tth111I XcmI
XhoI XmaI ZraI