Restriction map for
pFN21AE6789
(Linear) (Six-base) MAPSORT of: KIEE6789.seq Check: 8068 from: 1 to: 1740
KIEE6789.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
Acc65I G'GTAC_C
Cuts at: 0 725 1740
Size: 725 1015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1564 1740
Size: 679 885 176
Fragments arranged by size:
885 679 176
AleI CACnn'nnGTG
Cuts at: 0 1351 1600 1740
Size: 1351 249 140
Fragments arranged by size:
1351 249 140
AlwNI CAG_nnn'CTG
Cuts at: 0 177 1740
Size: 177 1563
ApaI G_GGCC'C
Cuts at: 0 757 1041 1740
Size: 757 284 699
Fragments arranged by size:
757 699 284
ApaLI G'TGCA_C
Cuts at: 0 204 599 1740
Size: 204 395 1141
Fragments arranged by size:
1141 395 204
AvaI C'yCGr_G
Cuts at: 0 1318 1740
Size: 1318 422
AvrII C'CTAG_G
Cuts at: 0 849 1740
Size: 849 891
BanI G'GyrC_C
Cuts at: 0 725 1466 1486 1740
Size: 725 741 20 254
Fragments arranged by size:
741 725 254 20
BanII G_rGCy'C
Cuts at: 0 325 757 1041 1091 1740
Size: 325 432 284 50 649
Fragments arranged by size:
649 432 325 284 50
BbeI G_GCGC'C
Cuts at: 0 1490 1740
Size: 1490 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 667 955 1740
Size: 667 288 785
Fragments arranged by size:
785 667 288
BbvCI CC'TCA_GC
Cuts at: 0 112 269 1740
Size: 112 157 1471
Fragments arranged by size:
1471 157 112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 150 184 1740
Size: 150 34 1556
Fragments arranged by size:
1556 150 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1282 1740
Size: 1282 458
BfrBI ATG'CAT
Cuts at: 0 1032 1740
Size: 1032 708
BglI GCCn_nnn'nGGC
Cuts at: 0 562 1495 1740
Size: 562 933 245
Fragments arranged by size:
933 562 245
BlpI GC'TnA_GC
Cuts at: 0 625 1259 1740
Size: 625 634 481
Fragments arranged by size:
634 625 481
Bme1580I G_kGCm'C
Cuts at: 0 208 603 654 757 1041 1740
Size: 208 395 51 103 284 699
Fragments arranged by size:
699 395 284 208 103 51
BmgBI CAC'GTC
Cuts at: 0 453 498 1740
Size: 453 45 1242
Fragments arranged by size:
1242 453 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 988 1147 1740
Size: 115 873 159 593
Fragments arranged by size:
873 593 159 115
Bpu10I CC'TnA_GC
Cuts at: 0 112 269 1214 1740
Size: 112 157 945 526
Fragments arranged by size:
945 526 157 112
BsaAI yAC'GTr
Cuts at: 0 1602 1740
Size: 1602 138
BsaHI Gr'CG_yC
Cuts at: 0 1474 1487 1740
Size: 1474 13 253
Fragments arranged by size:
1474 253 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 1450 1480 1546 1576 1740
Size: 307 30 1113 30 66 30 164
Fragments arranged by size:
1113 307 164 66 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 115 286 871 1740
Size: 112 3 171 585 869
Fragments arranged by size:
869 585 171 112 3
BseYI C'CCAG_C
Cuts at: 0 138 587 1040 1740
Size: 138 449 453 700
Fragments arranged by size:
700 453 449 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1740
Size: 763 977
BsiHKAI G_wGCw'C
Cuts at: 0 208 603 1091 1740
Size: 208 395 488 649
Fragments arranged by size:
649 488 395 208
BsmBI CGTCTCn'nnnn_
Cuts at: 0 780 1740
Size: 780 960
Bsp1286I G_dGCh'C
Cuts at: 0 208 325 603 654 757 1041 1091 1740
Size: 208 117 278 51 103 284 50 649
Fragments arranged by size:
649 284 278 208 117 103 51 50
BspHI T'CATG_A
Cuts at: 0 504 809 1740
Size: 504 305 931
Fragments arranged by size:
931 504 305
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 521 1740
Size: 521 1219
BsrBI CCG'CTC
Cuts at: 0 1218 1740
Size: 1218 522
BsrDI GCAATG_nn'
Cuts at: 0 789 1014 1740
Size: 789 225 726
Fragments arranged by size:
789 726 225
BsrFI r'CCGG_y
Cuts at: 0 435 671 1400 1740
Size: 435 236 729 340
Fragments arranged by size:
729 435 340 236
BssSI C'ACGA_G
Cuts at: 0 559 1740
Size: 559 1181
BstEII G'GTnAC_C
Cuts at: 0 1047 1740
Size: 1047 693
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1668 1740
Size: 1668 72
BstYI r'GATC_y
Cuts at: 0 173 788 1520 1697 1740
Size: 173 615 732 177 43
Fragments arranged by size:
732 615 177 173 43
BtgI C'CryG_G
Cuts at: 0 288 362 1068 1294 1411 1525 1740
Size: 288 74 706 226 117 114 215
Fragments arranged by size:
706 288 226 215 117 114 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 447 1740
Size: 447 1293
BtsI GCAGTG_nn'
Cuts at: 0 166 1740
Size: 166 1574
DraIII CAC_nnn'GTG
Cuts at: 0 201 1740
Size: 201 1539
EaeI y'GGCC_r
Cuts at: 0 1139 1402 1740
Size: 1139 263 338
Fragments arranged by size:
1139 338 263
EarI CTCTTCn'nnn_
Cuts at: 0 529 577 1063 1096 1333 1740
Size: 529 48 486 33 237 407
Fragments arranged by size:
529 486 407 237 48 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1194 1740
Size: 1194 546
EcoICRI GAG'CTC
Cuts at: 0 1089 1740
Size: 1089 651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 679 988 1147 1564 1740
Size: 115 564 309 159 417 176
Fragments arranged by size:
564 417 309 176 159 115
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 853 1740
Size: 181 672 887
Fragments arranged by size:
887 672 181
EcoO109I rG'GnC_Cy
Cuts at: 0 753 1037 1290 1708 1740
Size: 753 284 253 418 32
Fragments arranged by size:
753 418 284 253 32
FseI GG_CCGG'CC
Cuts at: 0 1404 1740
Size: 1404 336
HaeII r_GCGC'y
Cuts at: 0 333 1490 1740
Size: 333 1157 250
Fragments arranged by size:
1157 333 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1348 1380 1740
Size: 1348 32 360
Fragments arranged by size:
1348 360 32
KasI G'GCGC_C
Cuts at: 0 1486 1740
Size: 1486 254
KpnI G_GTAC'C
Cuts at: 0 729 1740
Size: 729 1011
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1740
Size: 284 1456
MscI TGG'CCA
Cuts at: 0 1141 1740
Size: 1141 599
MslI CAynn'nnrTG
Cuts at: 0 196 1351 1600 1740
Size: 196 1155 249 140
Fragments arranged by size:
1155 249 196 140
MspA1I CmG'CkG
Cuts at: 0 49 657 1044 1682 1740
Size: 49 608 387 638 58
Fragments arranged by size:
638 608 387 58 49
NaeI GCC'GGC
Cuts at: 0 673 1402 1740
Size: 673 729 338
Fragments arranged by size:
729 673 338
NarI GG'CG_CC
Cuts at: 0 1487 1740
Size: 1487 253
NcoI C'CATG_G
Cuts at: 0 288 1525 1740
Size: 288 1237 215
Fragments arranged by size:
1237 288 215
NdeI CA'TA_TG
Cuts at: 0 1098 1740
Size: 1098 642
NgoMIV G'CCGG_C
Cuts at: 0 671 1400 1740
Size: 671 729 340
Fragments arranged by size:
729 671 340
NsiI A_TGCA'T
Cuts at: 0 1034 1740
Size: 1034 706
NspI r_CATG'y
Cuts at: 0 67 85 1032 1740
Size: 67 18 947 708
Fragments arranged by size:
947 708 67 18
PasI CC'CwG_GG
Cuts at: 0 666 750 1223 1740
Size: 666 84 473 517
Fragments arranged by size:
666 517 473 84
PpuMI rG'GwC_Cy
Cuts at: 0 1290 1708 1740
Size: 1290 418 32
Fragments arranged by size:
1290 418 32
PspOMI G'GGCC_C
Cuts at: 0 753 1037 1740
Size: 753 284 703
Fragments arranged by size:
753 703 284
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1522 1554 1740
Size: 1522 32 186
Fragments arranged by size:
1522 186 32
PstI C_TGCA'G
Cuts at: 0 173 1580 1625 1740
Size: 173 1407 45 115
Fragments arranged by size:
1407 173 115 45
PvuII CAG'CTG
Cuts at: 0 657 1044 1740
Size: 657 387 696
Fragments arranged by size:
696 657 387
SacI G_AGCT'C
Cuts at: 0 1091 1740
Size: 1091 649
SanDI GG'GwC_CC
Cuts at: 0 1708 1740
Size: 1708 32
SapI GCTCTTCn'nnn_
Cuts at: 0 577 1096 1333 1740
Size: 577 519 237 407
Fragments arranged by size:
577 519 407 237
ScaI AGT'ACT
Cuts at: 0 385 1740
Size: 385 1355
SexAI A'CCwGG_T
Cuts at: 0 721 1667 1740
Size: 721 946 73
Fragments arranged by size:
946 721 73
SfcI C'TryA_G
Cuts at: 0 169 1576 1621 1740
Size: 169 1407 45 119
Fragments arranged by size:
1407 169 119 45
SfoI GGC'GCC
Cuts at: 0 1488 1740
Size: 1488 252
SphI G_CATG'C
Cuts at: 0 85 1032 1740
Size: 85 947 708
Fragments arranged by size:
947 708 85
StyI C'CwwG_G
Cuts at: 0 288 849 1349 1525 1740
Size: 288 561 500 176 215
Fragments arranged by size:
561 500 288 215 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1258 1740
Size: 1258 482
TatI w'GTAC_w
Cuts at: 0 383 860 1740
Size: 383 477 880
Fragments arranged by size:
880 477 383
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1450 1482 1740
Size: 158 32 1260 32 258
Fragments arranged by size:
1260 258 158 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 960 1740
Size: 960 780
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1386 1740
Size: 1386 354
Enzymes that do cut:
Acc65I AcuI AleI AlwNI ApaI ApaLI AvaI AvrII
BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstEII BstXI BstYI
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PpuMI PspOMI PsrI PstI
PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI
SphI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI BaeI
BamHI BclI BglII BmrI BmtI BplI BpuEI BsaI
BsaBI BsaWI BsiEI BsiWI BsmI BspEI BsrGI BssHII
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EagI EcoRI EcoRV FalI FspI FspAI HincII HindIII
HpaI MfeI MluI NheI NotI NruI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI RsrII SacII SalI SbfI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
XbaI XhoI XmaI XmnI ZraI