Restriction map for
pFN21AE6783
(Linear) (Six-base) MAPSORT of: KIEE6783.seq Check: 5853 from: 1 to: 1830
KIEE6783.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 134 192 1830
Size: 134 58 1638
Fragments arranged by size:
1638 134 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1573 1593 1830
Size: 1573 20 237
Fragments arranged by size:
1573 237 20
AlwNI CAG_nnn'CTG
Cuts at: 0 63 207 441 881 1830
Size: 63 144 234 440 949
Fragments arranged by size:
949 440 234 144 63
ApaI G_GGCC'C
Cuts at: 0 652 1830
Size: 652 1178
ApaLI G'TGCA_C
Cuts at: 0 306 728 1830
Size: 306 422 1102
Fragments arranged by size:
1102 422 306
ApoI r'AATT_y
Cuts at: 0 379 449 523 561 931 1470 1830
Size: 379 70 74 38 370 539 360
Fragments arranged by size:
539 379 370 360 74 70 38
BamHI G'GATC_C
Cuts at: 0 762 1830
Size: 762 1068
BanI G'GyrC_C
Cuts at: 0 229 819 1492 1830
Size: 229 590 673 338
Fragments arranged by size:
673 590 338 229
BanII G_rGCy'C
Cuts at: 0 652 1352 1830
Size: 652 700 478
Fragments arranged by size:
700 652 478
BbeI G_GCGC'C
Cuts at: 0 233 1830
Size: 233 1597
BbsI GAAGACnn'nnnn_
Cuts at: 0 1048 1268 1328 1599 1830
Size: 1048 220 60 271 231
Fragments arranged by size:
1048 271 231 220 60
BbvCI CC'TCA_GC
Cuts at: 0 1633 1830
Size: 1633 197
BclI T'GATC_A
Cuts at: 0 1232 1830
Size: 1232 598
BfrBI ATG'CAT
Cuts at: 0 977 1750 1830
Size: 977 773 80
Fragments arranged by size:
977 773 80
BglII A'GATC_T
Cuts at: 0 15 1830
Size: 15 1815
BlpI GC'TnA_GC
Cuts at: 0 442 1364 1628 1830
Size: 442 922 264 202
Fragments arranged by size:
922 442 264 202
Bme1580I G_kGCm'C
Cuts at: 0 267 310 652 732 1201 1830
Size: 267 43 342 80 469 629
Fragments arranged by size:
629 469 342 267 80 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 261 818 1581 1830
Size: 174 87 557 763 249
Fragments arranged by size:
763 557 249 174 87
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 436 1830
Size: 436 1394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 368 410 860 941 1109 1753 1830
Size: 40 328 42 450 81 168 644 77
Fragments arranged by size:
644 450 328 168 81 77 42 40
Bpu10I CC'TnA_GC
Cuts at: 0 1633 1830
Size: 1633 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 1391 1830
Size: 13 1378 439
Fragments arranged by size:
1378 439 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 65 1120 1185 1830
Size: 65 1055 65 645
Fragments arranged by size:
1055 645 65 65
BsaAI yAC'GTr
Cuts at: 0 588 1250 1830
Size: 588 662 580
Fragments arranged by size:
662 588 580
BsaHI Gr'CG_yC
Cuts at: 0 230 1830
Size: 230 1600
BsaWI w'CCGG_w
Cuts at: 0 137 1830
Size: 137 1693
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 459 489 788 818 1051 1081 1830
Size: 459 30 299 30 233 30 749
Fragments arranged by size:
749 459 299 233 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 112 1830
Size: 109 3 1718
Fragments arranged by size:
1718 109 3
BseYI C'CCAG_C
Cuts at: 0 207 1154 1623 1830
Size: 207 947 469 207
Fragments arranged by size:
947 469 207 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 516 1810 1830
Size: 205 311 1294 20
Fragments arranged by size:
1294 311 205 20
BsiEI CG_ry'CG
Cuts at: 0 1692 1830
Size: 1692 138
BsiHKAI G_wGCw'C
Cuts at: 0 27 173 310 732 1352 1830
Size: 27 146 137 422 620 478
Fragments arranged by size:
620 478 422 146 137 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 672 1830
Size: 672 1158
Bsp1286I G_dGCh'C
Cuts at: 0 27 173 267 310 652 732 1201 1352
Size: 27 146 94 43 342 80 469 151
Cuts at: 1352 1830
Size: 478
Fragments arranged by size:
478 469 342 151 146 94 80 43
27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 192 503 1167 1830
Size: 134 58 311 664 663
Fragments arranged by size:
664 663 311 134 58
BsrBI CCG'CTC
Cuts at: 0 1296 1830
Size: 1296 534
BsrGI T'GTAC_A
Cuts at: 0 883 1830
Size: 883 947
BssHII G'CGCG_C
Cuts at: 0 190 1830
Size: 190 1640
BstAPI GCAn_nnn'nTGC
Cuts at: 0 314 423 1830
Size: 314 109 1407
Fragments arranged by size:
1407 314 109
BstEII G'GTnAC_C
Cuts at: 0 1161 1830
Size: 1161 669
BstYI r'GATC_y
Cuts at: 0 15 762 1063 1830
Size: 15 747 301 767
Fragments arranged by size:
767 747 301 15
Bsu36I CC'TnA_GG
Cuts at: 0 283 1567 1830
Size: 283 1284 263
Fragments arranged by size:
1284 283 263
BtgI C'CryG_G
Cuts at: 0 554 644 1106 1830
Size: 554 90 462 724
Fragments arranged by size:
724 554 462 90
BtsI GCAGTG_nn'
Cuts at: 0 664 695 1830
Size: 664 31 1135
Fragments arranged by size:
1135 664 31
DraIII CAC_nnn'GTG
Cuts at: 0 258 314 1830
Size: 258 56 1516
Fragments arranged by size:
1516 258 56
DrdI GACnn_nn'nnGTC
Cuts at: 0 331 700 1830
Size: 331 369 1130
Fragments arranged by size:
1130 369 331
EaeI y'GGCC_r
Cuts at: 0 1244 1483 1830
Size: 1244 239 347
Fragments arranged by size:
1244 347 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1324 1830
Size: 1324 506
EcoICRI GAG'CTC
Cuts at: 0 1350 1830
Size: 1350 480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 368 410 860 941 1109 1573 1593
Size: 40 328 42 450 81 168 464 20
Cuts at: 1593 1753 1830
Size: 160 77
Fragments arranged by size:
464 450 328 168 160 81 77 42
40 20
EcoO109I rG'GnC_Cy
Cuts at: 0 60 290 649 1671 1830
Size: 60 230 359 1022 159
Fragments arranged by size:
1022 359 230 159 60
HaeII r_GCGC'y
Cuts at: 0 233 1830
Size: 233 1597
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1217 1224 1249 1256 1411 1443
Size: 1051 32 134 7 25 7 155 32
Cuts at: 1443 1738 1770 1830
Size: 295 32 60
Fragments arranged by size:
1051 295 155 134 60 32 32 32
25 7 7
HincII GTy'rAC
Cuts at: 0 705 1441 1655 1830
Size: 705 736 214 175
Fragments arranged by size:
736 705 214 175
HpaI GTT'AAC
Cuts at: 0 1655 1830
Size: 1655 175
KasI G'GCGC_C
Cuts at: 0 229 1830
Size: 229 1601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 390 409 1830
Size: 390 19 1421
Fragments arranged by size:
1421 390 19
MscI TGG'CCA
Cuts at: 0 1246 1830
Size: 1246 584
MspA1I CmG'CkG
Cuts at: 0 1158 1173 1286 1632 1735 1830
Size: 1158 15 113 346 103 95
Fragments arranged by size:
1158 346 113 103 95 15
NarI GG'CG_CC
Cuts at: 0 230 1830
Size: 230 1600
NsiI A_TGCA'T
Cuts at: 0 979 1752 1830
Size: 979 773 78
Fragments arranged by size:
979 773 78
NspI r_CATG'y
Cuts at: 0 1754 1830
Size: 1754 76
PasI CC'CwG_GG
Cuts at: 0 1071 1830
Size: 1071 759
PflMI CCAn_nnn'nTGG
Cuts at: 0 1802 1830
Size: 1802 28
PmlI CAC'GTG
Cuts at: 0 588 1250 1830
Size: 588 662 580
Fragments arranged by size:
662 588 580
PpuMI rG'GwC_Cy
Cuts at: 0 60 290 1830
Size: 60 230 1540
Fragments arranged by size:
1540 230 60
PshAI GACnn'nnGTC
Cuts at: 0 1090 1554 1819 1830
Size: 1090 464 265 11
Fragments arranged by size:
1090 464 265 11
PspOMI G'GGCC_C
Cuts at: 0 648 1830
Size: 648 1182
PstI C_TGCA'G
Cuts at: 0 131 250 1178 1830
Size: 131 119 928 652
Fragments arranged by size:
928 652 131 119
PvuII CAG'CTG
Cuts at: 0 1158 1173 1632 1735 1830
Size: 1158 15 459 103 95
Fragments arranged by size:
1158 459 103 95 15
SacI G_AGCT'C
Cuts at: 0 1352 1830
Size: 1352 478
SanDI GG'GwC_CC
Cuts at: 0 290 1830
Size: 290 1540
SfcI C'TryA_G
Cuts at: 0 127 246 1174 1830
Size: 127 119 928 656
Fragments arranged by size:
928 656 127 119
SfoI GGC'GCC
Cuts at: 0 231 1830
Size: 231 1599
SmlI C'TyrA_G
Cuts at: 0 28 1406 1830
Size: 28 1378 424
Fragments arranged by size:
1378 424 28
SphI G_CATG'C
Cuts at: 0 1754 1830
Size: 1754 76
StuI AGG'CCT
Cuts at: 0 985 1830
Size: 985 845
StyI C'CwwG_G
Cuts at: 0 1304 1830
Size: 1304 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 837 1830
Size: 837 993
TatI w'GTAC_w
Cuts at: 0 883 1830
Size: 883 947
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 711 743 1265 1297 1830
Size: 711 32 522 32 533
Fragments arranged by size:
711 533 522 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 660 1830
Size: 660 1170
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApaLI ApoI BamHI BanI
BanII BbeI BbsI BbvCI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrGI BssHII BstAPI BstEII
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HpaI KasI
MmeI MscI MspA1I NarI NsiI NspI PasI PflMI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SfcI SfoI SmlI SphI StuI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvaI
AvrII BaeI BcgI BciVI BglI BmgBI BmtI BsaBI
BsiWI BsmI BspEI BspHI BsrDI BsrFI BssSI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI EagI EarI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PciI PfoI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI