(Linear) (Six-base) MAPSORT of: KIEE6781.seq Check: 6446 from: 1 to: 1701 KIEE6781.orf With 182 enzymes: SgfI * March 16, 2015 11:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1446 1701 Size: 1446 255 AatII G_ACGT'C Cuts at: 0 28 1701 Size: 28 1673 Acc65I G'GTAC_C Cuts at: 0 322 1701 Size: 322 1379 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 94 266 1664 1701 Size: 34 60 172 1398 37 Fragments arranged by size: 1398 172 60 37 34 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 753 1303 1701 Size: 753 550 398 Fragments arranged by size: 753 550 398 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 266 298 1701 Size: 266 32 1403 Fragments arranged by size: 1403 266 32 AlwNI CAG_nnn'CTG Cuts at: 0 72 165 225 1128 1536 1701 Size: 72 93 60 903 408 165 Fragments arranged by size: 903 408 165 93 72 60 AvaI C'yCGr_G Cuts at: 0 377 1701 Size: 377 1324 AvrII C'CTAG_G Cuts at: 0 705 1701 Size: 705 996 BanI G'GyrC_C Cuts at: 0 322 329 602 1022 1148 1701 Size: 322 7 273 420 126 553 Fragments arranged by size: 553 420 322 273 126 7 BanII G_rGCy'C Cuts at: 0 368 392 805 1701 Size: 368 24 413 896 Fragments arranged by size: 896 413 368 24 BbeI G_GCGC'C Cuts at: 0 606 1701 Size: 606 1095 BbsI GAAGACnn'nnnn_ Cuts at: 0 28 1701 Size: 28 1673 BglI GCCn_nnn'nGGC Cuts at: 0 97 463 1016 1701 Size: 97 366 553 685 Fragments arranged by size: 685 553 366 97 BglII A'GATC_T Cuts at: 0 815 1701 Size: 815 886 Bme1580I G_kGCm'C Cuts at: 0 399 476 1182 1701 Size: 399 77 706 519 Fragments arranged by size: 706 519 399 77 BmrI ACTGGGnnnn_n' Cuts at: 0 286 1701 Size: 286 1415 BmtI G_CTAG'C Cuts at: 0 493 1701 Size: 493 1208 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 232 1701 Size: 232 1469 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 247 307 406 427 655 789 1150 Size: 202 45 60 99 21 228 134 361 Cuts at: 1150 1312 1543 1561 1701 Size: 162 231 18 140 Fragments arranged by size: 361 231 228 202 162 140 134 99 60 45 21 18 Bpu10I CC'TnA_GC Cuts at: 0 362 1701 Size: 362 1339 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1216 1291 1483 1701 Size: 1216 75 192 218 Fragments arranged by size: 1216 218 192 75 BsaI GGTCTCn'nnnn_ Cuts at: 0 1201 1701 Size: 1201 500 BsaBI GATnn'nnATC Cuts at: 0 814 1701 Size: 814 887 BsaHI Gr'CG_yC Cuts at: 0 9 25 603 1508 1701 Size: 9 16 578 905 193 Fragments arranged by size: 905 578 193 16 9 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1006 1036 1701 Size: 1006 30 665 Fragments arranged by size: 1006 665 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1600 1701 Size: 1600 101 BseYI C'CCAG_C Cuts at: 0 434 566 578 886 1701 Size: 434 132 12 308 815 Fragments arranged by size: 815 434 308 132 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 664 1321 1424 1701 Size: 556 108 657 103 277 Fragments arranged by size: 657 556 277 108 103 BsiHKAI G_wGCw'C Cuts at: 0 218 392 1307 1367 1577 1701 Size: 218 174 915 60 210 124 Fragments arranged by size: 915 218 210 174 124 60 BsmI GAATG_Cn' Cuts at: 0 654 994 1701 Size: 654 340 707 Fragments arranged by size: 707 654 340 Bsp1286I G_dGCh'C Cuts at: 0 218 368 392 399 476 805 1182 1307 Size: 218 150 24 7 77 329 377 125 Cuts at: 1307 1367 1577 1701 Size: 60 210 124 Fragments arranged by size: 377 329 218 210 150 125 124 77 60 24 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 917 1280 1446 1625 1701 Size: 917 363 166 179 76 Fragments arranged by size: 917 363 179 166 76 BsrBI CCG'CTC Cuts at: 0 420 551 1701 Size: 420 131 1150 Fragments arranged by size: 1150 420 131 BsrFI r'CCGG_y Cuts at: 0 881 1701 Size: 881 820 BstAPI GCAn_nnn'nTGC Cuts at: 0 1447 1536 1701 Size: 1447 89 165 Fragments arranged by size: 1447 165 89 BstEII G'GTnAC_C Cuts at: 0 1392 1624 1701 Size: 1392 232 77 Fragments arranged by size: 1392 232 77 BstYI r'GATC_y Cuts at: 0 815 1353 1701 Size: 815 538 348 Fragments arranged by size: 815 538 348 Bsu36I CC'TnA_GG Cuts at: 0 611 1701 Size: 611 1090 BtgI C'CryG_G Cuts at: 0 1238 1701 Size: 1238 463 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 989 1701 Size: 989 712 BtsI GCAGTG_nn' Cuts at: 0 429 730 1577 1701 Size: 429 301 847 124 Fragments arranged by size: 847 429 301 124 DraI TTT'AAA Cuts at: 0 1001 1701 Size: 1001 700 DrdI GACnn_nn'nnGTC Cuts at: 0 237 1701 Size: 237 1464 EaeI y'GGCC_r Cuts at: 0 455 1701 Size: 455 1246 EarI CTCTTCn'nnn_ Cuts at: 0 26 242 479 540 1701 Size: 26 216 237 61 1161 Fragments arranged by size: 1161 237 216 61 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 55 1701 Size: 55 1646 EcoICRI GAG'CTC Cuts at: 0 390 1701 Size: 390 1311 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 94 202 247 266 307 406 427 Size: 34 60 108 45 19 41 99 21 Cuts at: 427 655 789 1150 1312 1543 1561 1664 1701 Size: 228 134 361 162 231 18 103 37 Fragments arranged by size: 361 231 228 162 134 108 103 99 60 45 41 37 34 21 19 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 610 1596 1701 Size: 610 986 105 Fragments arranged by size: 986 610 105 EcoO109I rG'GnC_Cy Cuts at: 0 863 1701 Size: 863 838 FspI TGC'GCA Cuts at: 0 1276 1701 Size: 1276 425 HaeII r_GCGC'y Cuts at: 0 117 606 1701 Size: 117 489 1095 Fragments arranged by size: 1095 489 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1090 1122 1701 Size: 1090 32 579 Fragments arranged by size: 1090 579 32 KasI G'GCGC_C Cuts at: 0 602 1701 Size: 602 1099 KpnI G_GTAC'C Cuts at: 0 326 1701 Size: 326 1375 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 551 1701 Size: 551 1150 MscI TGG'CCA Cuts at: 0 457 1701 Size: 457 1244 MspA1I CmG'CkG Cuts at: 0 225 570 1383 1632 1701 Size: 225 345 813 249 69 Fragments arranged by size: 813 345 249 225 69 NarI GG'CG_CC Cuts at: 0 603 1701 Size: 603 1098 NheI G'CTAG_C Cuts at: 0 489 1701 Size: 489 1212 NspI r_CATG'y Cuts at: 0 748 1701 Size: 748 953 PflMI CCAn_nnn'nTGG Cuts at: 0 165 194 225 1644 1701 Size: 165 29 31 1419 57 Fragments arranged by size: 1419 165 57 31 29 PfoI T'CCnGG_A Cuts at: 0 284 404 805 1701 Size: 284 120 401 896 Fragments arranged by size: 896 401 284 120 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1370 1402 1701 Size: 1370 32 299 Fragments arranged by size: 1370 299 32 PpuMI rG'GwC_Cy Cuts at: 0 863 1701 Size: 863 838 PstI C_TGCA'G Cuts at: 0 483 1094 1124 1217 1475 1532 1572 1622 Size: 483 611 30 93 258 57 40 50 Cuts at: 1622 1701 Size: 79 Fragments arranged by size: 611 483 258 93 79 57 50 40 30 PvuII CAG'CTG Cuts at: 0 225 570 1383 1701 Size: 225 345 813 318 Fragments arranged by size: 813 345 318 225 SacI G_AGCT'C Cuts at: 0 392 1701 Size: 392 1309 SapI GCTCTTCn'nnn_ Cuts at: 0 26 479 1701 Size: 26 453 1222 Fragments arranged by size: 1222 453 26 SbfI CC_TGCA'GG Cuts at: 0 1622 1701 Size: 1622 79 ScaI AGT'ACT Cuts at: 0 1219 1701 Size: 1219 482 SfcI C'TryA_G Cuts at: 0 479 1090 1120 1213 1471 1528 1568 1618 Size: 479 611 30 93 258 57 40 50 Cuts at: 1618 1701 Size: 83 Fragments arranged by size: 611 479 258 93 83 57 50 40 30 SfoI GGC'GCC Cuts at: 0 604 1701 Size: 604 1097 SmlI C'TyrA_G Cuts at: 0 1231 1306 1462 1701 Size: 1231 75 156 239 Fragments arranged by size: 1231 239 156 75 StuI AGG'CCT Cuts at: 0 763 1701 Size: 763 938 StyI C'CwwG_G Cuts at: 0 705 1701 Size: 705 996 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 109 1411 1701 Size: 109 1302 290 Fragments arranged by size: 1302 290 109 TatI w'GTAC_w Cuts at: 0 1217 1701 Size: 1217 484 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1006 1038 1701 Size: 1006 32 663 Fragments arranged by size: 1006 663 32 Tth111I GACn'n_nGTC Cuts at: 0 1507 1701 Size: 1507 194 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 586 1497 1701 Size: 586 911 204 Fragments arranged by size: 911 586 204 XmnI GAAnn'nnTTC Cuts at: 0 652 1701 Size: 652 1049 ZraI GAC'GTC Cuts at: 0 26 1701 Size: 26 1675 Enzymes that do cut: AarI AatII Acc65I AcuI AlfI AloI AlwNI AvaI AvrII BanI BanII BbeI BbsI BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI KpnI MmeI MscI MspA1I NarI NheI NspI PflMI PfoI PpiI PpuMI PstI PvuII SacI SapI SbfI ScaI SfcI SfoI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BsaAI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I ClaI CspCI DraIII EagI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI