Restriction map for
pFN21AE6775
(Linear) (Six-base) MAPSORT of: KIEE6775.seq Check: 6940 from: 1 to: 1905
KIEE6775.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 894 1888 1905
Size: 894 994 17
Fragments arranged by size:
994 894 17
AccI GT'mk_AC
Cuts at: 0 64 1638 1905
Size: 64 1574 267
Fragments arranged by size:
1574 267 64
Acc65I G'GTAC_C
Cuts at: 0 839 1905
Size: 839 1066
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1054 1062 1193 1905
Size: 607 447 8 131 712
Fragments arranged by size:
712 607 447 131 8
AleI CACnn'nnGTG
Cuts at: 0 52 1609 1905
Size: 52 1557 296
Fragments arranged by size:
1557 296 52
AlwNI CAG_nnn'CTG
Cuts at: 0 514 621 1905
Size: 514 107 1284
Fragments arranged by size:
1284 514 107
AvaI C'yCGr_G
Cuts at: 0 42 634 1178 1905
Size: 42 592 544 727
Fragments arranged by size:
727 592 544 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 831 864 1905
Size: 831 33 1041
Fragments arranged by size:
1041 831 33
BamHI G'GATC_C
Cuts at: 0 738 1905
Size: 738 1167
BanI G'GyrC_C
Cuts at: 0 839 1905
Size: 839 1066
BanII G_rGCy'C
Cuts at: 0 131 1061 1391 1441 1905
Size: 131 930 330 50 464
Fragments arranged by size:
930 464 330 131 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 783 1145 1905
Size: 783 362 760
Fragments arranged by size:
783 760 362
BbvCI CC'TCA_GC
Cuts at: 0 21 1905
Size: 21 1884
BciVI GTATCCnnnnn_n'
Cuts at: 0 974 1905
Size: 974 931
BglI GCCn_nnn'nGGC
Cuts at: 0 932 1046 1551 1905
Size: 932 114 505 354
Fragments arranged by size:
932 505 354 114
BglII A'GATC_T
Cuts at: 0 272 1905
Size: 272 1633
Bme1580I G_kGCm'C
Cuts at: 0 49 1905
Size: 49 1856
BmrI ACTGGGnnnn_n'
Cuts at: 0 1655 1684 1905
Size: 1655 29 221
Fragments arranged by size:
1655 221 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 496 1905
Size: 496 1409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1425 1873 1905
Size: 418 1007 448 32
Fragments arranged by size:
1007 448 418 32
Bpu10I CC'TnA_GC
Cuts at: 0 21 827 986 1905
Size: 21 806 159 919
Fragments arranged by size:
919 806 159 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 1888 1905
Size: 1888 17
BsaBI GATnn'nnATC
Cuts at: 0 460 1411 1905
Size: 460 951 494
Fragments arranged by size:
951 494 460
BsaWI w'CCGG_w
Cuts at: 0 207 1905
Size: 207 1698
BseYI C'CCAG_C
Cuts at: 0 149 707 1314 1384 1812 1905
Size: 149 558 607 70 428 93
Fragments arranged by size:
607 558 428 149 93 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 853 1905
Size: 323 530 1052
Fragments arranged by size:
1052 530 323
BsiEI CG_ry'CG
Cuts at: 0 717 1905
Size: 717 1188
BsiHKAI G_wGCw'C
Cuts at: 0 19 482 875 1596 1905
Size: 19 463 393 721 309
Fragments arranged by size:
721 463 393 309 19
BsmI GAATG_Cn'
Cuts at: 0 974 1658 1905
Size: 974 684 247
Fragments arranged by size:
974 684 247
Bsp1286I G_dGCh'C
Cuts at: 0 19 49 131 482 875 1061 1391 1441
Size: 19 30 82 351 393 186 330 50
Cuts at: 1441 1596 1905
Size: 155 309
Fragments arranged by size:
393 351 330 309 186 155 82 50
30 19
BspHI T'CATG_A
Cuts at: 0 794 1905
Size: 794 1111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 894 1888 1905
Size: 345 549 994 17
Fragments arranged by size:
994 549 345 17
BsrDI GCAATG_nn'
Cuts at: 0 1064 1905
Size: 1064 841
BsrFI r'CCGG_y
Cuts at: 0 155 1661 1905
Size: 155 1506 244
Fragments arranged by size:
1506 244 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1845 1905
Size: 1845 60
BstYI r'GATC_y
Cuts at: 0 272 738 1026 1905
Size: 272 466 288 879
Fragments arranged by size:
879 466 288 272
BtgI C'CryG_G
Cuts at: 0 245 698 1838 1905
Size: 245 453 1140 67
Fragments arranged by size:
1140 453 245 67
BtsI GCAGTG_nn'
Cuts at: 0 515 893 1743 1905
Size: 515 378 850 162
Fragments arranged by size:
850 515 378 162
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1596 1631 1905
Size: 1596 35 274
Fragments arranged by size:
1596 274 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1006 1905
Size: 1006 899
EaeI y'GGCC_r
Cuts at: 0 227 248 714 1758 1905
Size: 227 21 466 1044 147
Fragments arranged by size:
1044 466 227 147 21
EagI C'GGCC_G
Cuts at: 0 714 1905
Size: 714 1191
EarI CTCTTCn'nnn_
Cuts at: 0 1066 1081 1327 1905
Size: 1066 15 246 578
Fragments arranged by size:
1066 578 246 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 1905
Size: 199 1706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 607 1054 1062 1193 1425 1873 1905
Size: 418 189 447 8 131 232 448 32
Fragments arranged by size:
448 447 418 232 189 131 32 8
EcoO109I rG'GnC_Cy
Cuts at: 0 287 1038 1261 1905
Size: 287 751 223 644
Fragments arranged by size:
751 644 287 223
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1620 1652 1905
Size: 1620 32 253
Fragments arranged by size:
1620 253 32
KpnI G_GTAC'C
Cuts at: 0 843 1905
Size: 843 1062
MscI TGG'CCA
Cuts at: 0 229 250 1905
Size: 229 21 1655
Fragments arranged by size:
1655 229 21
MslI CAynn'nnrTG
Cuts at: 0 52 1519 1609 1905
Size: 52 1467 90 296
Fragments arranged by size:
1467 296 90 52
MspA1I CmG'CkG
Cuts at: 0 642 837 1082 1311 1560 1905
Size: 642 195 245 229 249 345
Fragments arranged by size:
642 345 249 245 229 195
NaeI GCC'GGC
Cuts at: 0 157 1905
Size: 157 1748
NcoI C'CATG_G
Cuts at: 0 1838 1905
Size: 1838 67
NgoMIV G'CCGG_C
Cuts at: 0 155 1905
Size: 155 1750
NspI r_CATG'y
Cuts at: 0 63 1449 1905
Size: 63 1386 456
Fragments arranged by size:
1386 456 63
PasI CC'CwG_GG
Cuts at: 0 546 1905
Size: 546 1359
PflMI CCAn_nnn'nTGG
Cuts at: 0 621 1905
Size: 621 1284
PfoI T'CCnGG_A
Cuts at: 0 1889 1905
Size: 1889 16
PpuMI rG'GwC_Cy
Cuts at: 0 287 1261 1905
Size: 287 974 644
Fragments arranged by size:
974 644 287
PshAI GACnn'nnGTC
Cuts at: 0 622 1905
Size: 622 1283
PstI C_TGCA'G
Cuts at: 0 1274 1559 1750 1905
Size: 1274 285 191 155
Fragments arranged by size:
1274 285 191 155
PvuII CAG'CTG
Cuts at: 0 642 1905
Size: 642 1263
RsrII CG'GwC_CG
Cuts at: 0 186 1905
Size: 186 1719
SanDI GG'GwC_CC
Cuts at: 0 287 1905
Size: 287 1618
SapI GCTCTTCn'nnn_
Cuts at: 0 1066 1905
Size: 1066 839
SfcI C'TryA_G
Cuts at: 0 1270 1555 1746 1905
Size: 1270 285 191 159
Fragments arranged by size:
1270 285 191 159
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1046 1905
Size: 1046 859
SmaI CCC'GGG
Cuts at: 0 1180 1905
Size: 1180 725
SphI G_CATG'C
Cuts at: 0 1449 1905
Size: 1449 456
SrfI GCCC'GGGC
Cuts at: 0 1180 1905
Size: 1180 725
SspI AAT'ATT
Cuts at: 0 295 1905
Size: 295 1610
StuI AGG'CCT
Cuts at: 0 1049 1202 1905
Size: 1049 153 703
Fragments arranged by size:
1049 703 153
StyI C'CwwG_G
Cuts at: 0 668 773 1153 1329 1838 1905
Size: 668 105 380 176 509 67
Fragments arranged by size:
668 509 380 176 105 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 247 568 1905
Size: 247 321 1337
Fragments arranged by size:
1337 321 247
TatI w'GTAC_w
Cuts at: 0 1013 1905
Size: 1013 892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 142 1614 1774 1905
Size: 142 1472 160 131
Fragments arranged by size:
1472 160 142 131
XmaI C'CCGG_G
Cuts at: 0 1178 1905
Size: 1178 727
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlwNI AvaI BaeI
BamHI BanI BanII BbsI BbvCI BciVI BglI BglII
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaBI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BstXI BstYI BtgI BtsI CspCI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I Hin4I KpnI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PflMI PfoI PpuMI PshAI PstI PvuII RsrII SanDI
SapI SfcI SfiI SmaI SphI SrfI SspI StuI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BbeI BcgI BclI BfrBI BlpI BmgBI BmtI BpuEI
BsaAI BsaHI BsaXI BseRI BsiWI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DraIII EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI SacI SacII SalI SbfI
ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI