Restriction map for
pFN21AE6773
(Linear) (Six-base) MAPSORT of: KIEE6773.seq Check: 3664 from: 1 to: 1800
KIEE6773.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 252 1800
Size: 252 1548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 620 676 1127 1184 1800
Size: 374 246 56 451 57 616
Fragments arranged by size:
616 451 374 246 57 56
AleI CACnn'nnGTG
Cuts at: 0 343 479 1800
Size: 343 136 1321
Fragments arranged by size:
1321 343 136
AlwNI CAG_nnn'CTG
Cuts at: 0 151 1800
Size: 151 1649
ApoI r'AATT_y
Cuts at: 0 799 1114 1378 1469 1790 1800
Size: 799 315 264 91 321 10
Fragments arranged by size:
799 321 315 264 91 10
BanI G'GyrC_C
Cuts at: 0 315 585 1273 1800
Size: 315 270 688 527
Fragments arranged by size:
688 527 315 270
BanII G_rGCy'C
Cuts at: 0 125 187 269 598 1800
Size: 125 62 82 329 1202
Fragments arranged by size:
1202 329 125 82 62
BbeI G_GCGC'C
Cuts at: 0 589 1800
Size: 589 1211
BbsI GAAGACnn'nnnn_
Cuts at: 0 557 664 1320 1800
Size: 557 107 656 480
Fragments arranged by size:
656 557 480 107
BclI T'GATC_A
Cuts at: 0 690 1800
Size: 690 1110
BglI GCCn_nnn'nGGC
Cuts at: 0 1243 1800
Size: 1243 557
BglII A'GATC_T
Cuts at: 0 1073 1800
Size: 1073 727
Bme1580I G_kGCm'C
Cuts at: 0 991 1800
Size: 991 809
BmgBI CAC'GTC
Cuts at: 0 495 1488 1800
Size: 495 993 312
Fragments arranged by size:
993 495 312
BmrI ACTGGGnnnn_n'
Cuts at: 0 1397 1800
Size: 1397 403
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 570 1800
Size: 570 1230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 995 1774 1800
Size: 859 136 779 26
Fragments arranged by size:
859 779 136 26
Bpu10I CC'TnA_GC
Cuts at: 0 641 1800
Size: 641 1159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1006 1800
Size: 640 366 794
Fragments arranged by size:
794 640 366
BsaHI Gr'CG_yC
Cuts at: 0 156 205 586 1800
Size: 156 49 381 1214
Fragments arranged by size:
1214 381 156 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 1800
Size: 28 1772
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 404 1546 1549 1800
Size: 404 1142 3 251
Fragments arranged by size:
1142 404 251 3
BseYI C'CCAG_C
Cuts at: 0 358 610 1800
Size: 358 252 1190
Fragments arranged by size:
1190 358 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 1800
Size: 1331 469
BsiHKAI G_wGCw'C
Cuts at: 0 1458 1800
Size: 1458 342
BsmI GAATG_Cn'
Cuts at: 0 642 1800
Size: 642 1158
Bsp1286I G_dGCh'C
Cuts at: 0 125 187 269 598 991 1458 1800
Size: 125 62 82 329 393 467 342
Fragments arranged by size:
467 393 342 329 125 82 62
BspHI T'CATG_A
Cuts at: 0 1194 1800
Size: 1194 606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 252 659 1353 1800
Size: 252 407 694 447
Fragments arranged by size:
694 447 407 252
BsrDI GCAATG_nn'
Cuts at: 0 1308 1800
Size: 1308 492
BsrFI r'CCGG_y
Cuts at: 0 582 1800
Size: 582 1218
BsrGI T'GTAC_A
Cuts at: 0 1641 1800
Size: 1641 159
BssSI C'ACGA_G
Cuts at: 0 339 1800
Size: 339 1461
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1462 1800
Size: 1462 338
BstXI CCAn_nnnn'nTGG
Cuts at: 0 481 1800
Size: 481 1319
BstYI r'GATC_y
Cuts at: 0 1073 1800
Size: 1073 727
BtgI C'CryG_G
Cuts at: 0 67 221 259 1318 1800
Size: 67 154 38 1059 482
Fragments arranged by size:
1059 482 154 67 38
EaeI y'GGCC_r
Cuts at: 0 64 296 1800
Size: 64 232 1504
Fragments arranged by size:
1504 232 64
EarI CTCTTCn'nnn_
Cuts at: 0 276 938 1130 1142 1160 1514 1535 1800
Size: 276 662 192 12 18 354 21 265
Fragments arranged by size:
662 354 276 265 192 21 18 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 620 676 859 995 1127 1184 1774
Size: 374 246 56 183 136 132 57 590
Cuts at: 1774 1800
Size: 26
Fragments arranged by size:
590 374 246 183 136 132 57 56
26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1427 1800
Size: 1427 373
EcoO109I rG'GnC_Cy
Cuts at: 0 1236 1800
Size: 1236 564
HaeII r_GCGC'y
Cuts at: 0 589 1800
Size: 589 1211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 97 129 293 325 354 386 1065
Size: 30 67 32 164 32 29 32 679
Cuts at: 1065 1097 1800
Size: 32 703
Fragments arranged by size:
703 679 164 67 32 32 32 32
30 29
HincII GTy'rAC
Cuts at: 0 710 747 897 1800
Size: 710 37 150 903
Fragments arranged by size:
903 710 150 37
KasI G'GCGC_C
Cuts at: 0 585 1800
Size: 585 1215
MfeI C'AATT_G
Cuts at: 0 1626 1800
Size: 1626 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 859 1765 1800
Size: 308 551 906 35
Fragments arranged by size:
906 551 308 35
MslI CAynn'nnrTG
Cuts at: 0 343 479 1199 1597 1800
Size: 343 136 720 398 203
Fragments arranged by size:
720 398 343 203 136
MspA1I CmG'CkG
Cuts at: 0 223 261 1800
Size: 223 38 1539
Fragments arranged by size:
1539 223 38
NaeI GCC'GGC
Cuts at: 0 584 1800
Size: 584 1216
NarI GG'CG_CC
Cuts at: 0 586 1800
Size: 586 1214
NcoI C'CATG_G
Cuts at: 0 67 1318 1800
Size: 67 1251 482
Fragments arranged by size:
1251 482 67
NdeI CA'TA_TG
Cuts at: 0 840 1760 1800
Size: 840 920 40
Fragments arranged by size:
920 840 40
NgoMIV G'CCGG_C
Cuts at: 0 582 1800
Size: 582 1218
NspI r_CATG'y
Cuts at: 0 618 1800
Size: 618 1182
PflMI CCAn_nnn'nTGG
Cuts at: 0 151 906 1747 1800
Size: 151 755 841 53
Fragments arranged by size:
841 755 151 53
PshAI GACnn'nnGTC
Cuts at: 0 159 1800
Size: 159 1641
SacII CC_GC'GG
Cuts at: 0 224 262 1800
Size: 224 38 1538
Fragments arranged by size:
1538 224 38
SapI GCTCTTCn'nnn_
Cuts at: 0 938 1800
Size: 938 862
SfoI GGC'GCC
Cuts at: 0 587 1800
Size: 587 1213
SmlI C'TyrA_G
Cuts at: 0 619 1021 1800
Size: 619 402 779
Fragments arranged by size:
779 619 402
SphI G_CATG'C
Cuts at: 0 618 1800
Size: 618 1182
SspI AAT'ATT
Cuts at: 0 100 762 1800
Size: 100 662 1038
Fragments arranged by size:
1038 662 100
StyI C'CwwG_G
Cuts at: 0 67 125 178 1318 1800
Size: 67 58 53 1140 482
Fragments arranged by size:
1140 482 67 58 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 202 1058 1311 1800
Size: 202 856 253 489
Fragments arranged by size:
856 489 253 202
TatI w'GTAC_w
Cuts at: 0 1641 1800
Size: 1641 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 327 359 1800
Size: 327 32 1441
Fragments arranged by size:
1441 327 32
XbaI T'CTAG_A
Cuts at: 0 193 1800
Size: 193 1607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 1316 1800
Size: 207 1109 484
Fragments arranged by size:
1109 484 207
XmnI GAAnn'nnTTC
Cuts at: 0 1381 1800
Size: 1381 419
Enzymes that do cut:
AarI AcuI AleI AlwNI ApoI BanI BanII BbeI
BbsI BclI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI BtgI EaeI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MfeI MmeI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PflMI PshAI SacII SapI SfoI SmlI SphI SspI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI
BlpI BmtI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BsrBI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MluI MscI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI Tth111I XhoI XmaI ZraI