Restriction map for
pFN21AE6771
(Linear) (Six-base) MAPSORT of: KIEE6771.seq Check: 1854 from: 1 to: 1689
KIEE6771.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
Acc65I G'GTAC_C
Cuts at: 0 709 1091 1689
Size: 709 382 598
Fragments arranged by size:
709 598 382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1265 1665 1689
Size: 941 324 400 24
Fragments arranged by size:
941 400 324 24
AflII C'TTAA_G
Cuts at: 0 973 1689
Size: 973 716
AhdI GACnn_n'nnGTC
Cuts at: 0 279 1689
Size: 279 1410
AlwNI CAG_nnn'CTG
Cuts at: 0 445 1689
Size: 445 1244
ApaI G_GGCC'C
Cuts at: 0 468 1689
Size: 468 1221
AvaI C'yCGr_G
Cuts at: 0 212 1472 1689
Size: 212 1260 217
Fragments arranged by size:
1260 217 212
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1659 1689
Size: 1659 30
BanI G'GyrC_C
Cuts at: 0 94 339 427 709 1091 1264 1689
Size: 94 245 88 282 382 173 425
Fragments arranged by size:
425 382 282 245 173 94 88
BanII G_rGCy'C
Cuts at: 0 115 468 643 1330 1473 1523 1689
Size: 115 353 175 687 143 50 166
Fragments arranged by size:
687 353 175 166 143 115 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 1669 1689
Size: 596 1073 20
Fragments arranged by size:
1073 596 20
BbvCI CC'TCA_GC
Cuts at: 0 770 1689
Size: 770 919
BclI T'GATC_A
Cuts at: 0 837 1689
Size: 837 852
BfrBI ATG'CAT
Cuts at: 0 392 1689
Size: 392 1297
BglI GCCn_nnn'nGGC
Cuts at: 0 1285 1689
Size: 1285 404
Bme1580I G_kGCm'C
Cuts at: 0 39 97 468 1296 1689
Size: 39 58 371 828 393
Fragments arranged by size:
828 393 371 58 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 297 433 761 834 1090 1689
Size: 297 136 328 73 256 599
Fragments arranged by size:
599 328 297 256 136 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1476 1689
Size: 830 646 213
Fragments arranged by size:
830 646 213
Bpu10I CC'TnA_GC
Cuts at: 0 770 1689
Size: 770 919
BsaI GGTCTCn'nnnn_
Cuts at: 0 287 557 1630 1689
Size: 287 270 1073 59
Fragments arranged by size:
1073 287 270 59
BsaBI GATnn'nnATC
Cuts at: 0 839 1689
Size: 839 850
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 650 680 1689
Size: 650 30 1009
Fragments arranged by size:
1009 650 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 536 671 993 1436 1689
Size: 349 187 135 322 443 253
Fragments arranged by size:
443 349 322 253 187 135
BseYI C'CCAG_C
Cuts at: 0 90 98 1689
Size: 90 8 1591
Fragments arranged by size:
1591 90 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 1658 1689
Size: 630 1028 31
Fragments arranged by size:
1028 630 31
BsiEI CG_ry'CG
Cuts at: 0 31 1689
Size: 31 1658
BsiHKAI G_wGCw'C
Cuts at: 0 219 1689
Size: 219 1470
BsmI GAATG_Cn'
Cuts at: 0 394 1689
Size: 394 1295
Bsp1286I G_dGCh'C
Cuts at: 0 39 97 115 219 468 643 1296 1330
Size: 39 58 18 104 249 175 653 34
Cuts at: 1330 1473 1523 1689
Size: 143 50 166
Fragments arranged by size:
653 249 175 166 143 104 58 50
39 34 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 301 1689
Size: 301 1388
BsrDI GCAATG_nn'
Cuts at: 0 795 1001 1689
Size: 795 206 688
Fragments arranged by size:
795 688 206
BssHII G'CGCG_C
Cuts at: 0 60 1689
Size: 60 1629
BstEII G'GTnAC_C
Cuts at: 0 313 1689
Size: 313 1376
BstXI CCAn_nnnn'nTGG
Cuts at: 0 720 1275 1443 1689
Size: 720 555 168 246
Fragments arranged by size:
720 555 246 168
BstYI r'GATC_y
Cuts at: 0 774 1689
Size: 774 915
Bsu36I CC'TnA_GG
Cuts at: 0 13 1689
Size: 13 1676
BtgI C'CryG_G
Cuts at: 0 674 1689
Size: 674 1015
BtsI GCAGTG_nn'
Cuts at: 0 635 1689
Size: 635 1054
DraI TTT'AAA
Cuts at: 0 1514 1689
Size: 1514 175
EaeI y'GGCC_r
Cuts at: 0 28 573 1277 1393 1445 1689
Size: 28 545 704 116 52 244
Fragments arranged by size:
704 545 244 116 52 28
EagI C'GGCC_G
Cuts at: 0 28 1689
Size: 28 1661
EarI CTCTTCn'nnn_
Cuts at: 0 366 917 1535 1689
Size: 366 551 618 154
Fragments arranged by size:
618 551 366 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 941 1265 1476 1665 1689
Size: 830 111 324 211 189 24
Fragments arranged by size:
830 324 211 189 111 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 1689
Size: 124 1565
EcoO109I rG'GnC_Cy
Cuts at: 0 727 1689
Size: 727 962
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 999 1686 1689
Size: 999 687 3
Fragments arranged by size:
999 687 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 265 297 1581 1613 1689
Size: 97 32 136 32 1284 32 76
Fragments arranged by size:
1284 136 97 76 32 32 32
KpnI G_GTAC'C
Cuts at: 0 713 1095 1689
Size: 713 382 594
Fragments arranged by size:
713 594 382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1089 1689
Size: 1089 600
MscI TGG'CCA
Cuts at: 0 1395 1447 1689
Size: 1395 52 242
Fragments arranged by size:
1395 242 52
MspA1I CmG'CkG
Cuts at: 0 1282 1689
Size: 1282 407
NcoI C'CATG_G
Cuts at: 0 674 1689
Size: 674 1015
NsiI A_TGCA'T
Cuts at: 0 394 1689
Size: 394 1295
PasI CC'CwG_GG
Cuts at: 0 277 1689
Size: 277 1412
PflMI CCAn_nnn'nTGG
Cuts at: 0 349 1689
Size: 349 1340
PpuMI rG'GwC_Cy
Cuts at: 0 727 1689
Size: 727 962
PspOMI G'GGCC_C
Cuts at: 0 464 1689
Size: 464 1225
SapI GCTCTTCn'nnn_
Cuts at: 0 917 1689
Size: 917 772
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1285 1689
Size: 1285 404
SmaI CCC'GGG
Cuts at: 0 1474 1689
Size: 1474 215
SmlI C'TyrA_G
Cuts at: 0 973 1689
Size: 973 716
SpeI A'CTAG_T
Cuts at: 0 153 1689
Size: 153 1536
SspI AAT'ATT
Cuts at: 0 615 1085 1185 1689
Size: 615 470 100 504
Fragments arranged by size:
615 504 470 100
StyI C'CwwG_G
Cuts at: 0 674 734 1256 1689
Size: 674 60 522 433
Fragments arranged by size:
674 522 433 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 1689
Size: 112 1577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 650 682 1689
Size: 650 32 1007
Fragments arranged by size:
1007 650 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1617 1689
Size: 1617 72
XmaI C'CCGG_G
Cuts at: 0 1472 1689
Size: 1472 217
Enzymes that do cut:
Acc65I AcuI AflII AhdI AlwNI ApaI AvaI BaeI
BanI BanII BbsI BbvCI BclI BfrBI BglI Bme1580I
BmrI BpmI Bpu10I BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BssHII
BstEII BstXI BstYI Bsu36I BtgI BtsI DraI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FalI Hin4I KpnI
MmeI MscI MspA1I NcoI NsiI PasI PflMI PpuMI
PspOMI SapI SfiI SmaI SmlI SpeI SspI StyI
TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BcgI BciVI BglII BlpI BmgBI BmtI
BplI BpuEI BsaAI BsaHI BsaWI BsiWI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SnaBI SphI SrfI
StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI