Restriction map for
pFN21AE6770
(Linear) (Six-base) MAPSORT of: KIEE6770.seq Check: 694 from: 1 to: 1665
KIEE6770.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 984 1639 1665
Size: 984 655 26
Fragments arranged by size:
984 655 26
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 640 980 1665
Size: 528 112 340 685
Fragments arranged by size:
685 528 340 112
AflII C'TTAA_G
Cuts at: 0 168 1665
Size: 168 1497
AflIII A'CryG_T
Cuts at: 0 1084 1511 1665
Size: 1084 427 154
Fragments arranged by size:
1084 427 154
AleI CACnn'nnGTG
Cuts at: 0 292 545 1665
Size: 292 253 1120
Fragments arranged by size:
1120 292 253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1016 1665
Size: 1016 649
AlwNI CAG_nnn'CTG
Cuts at: 0 672 1069 1656 1665
Size: 672 397 587 9
Fragments arranged by size:
672 587 397 9
ApoI r'AATT_y
Cuts at: 0 244 310 319 402 1074 1665
Size: 244 66 9 83 672 591
Fragments arranged by size:
672 591 244 83 66 9
AvaI C'yCGr_G
Cuts at: 0 1339 1665
Size: 1339 326
BamHI G'GATC_C
Cuts at: 0 1560 1665
Size: 1560 105
BanI G'GyrC_C
Cuts at: 0 112 1665
Size: 112 1553
BanII G_rGCy'C
Cuts at: 0 335 498 1319 1665
Size: 335 163 821 346
Fragments arranged by size:
821 346 335 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 221 405 557 1665
Size: 221 184 152 1108
Fragments arranged by size:
1108 221 184 152
BciVI GTATCCnnnnn_n'
Cuts at: 0 742 1665
Size: 742 923
BglII A'GATC_T
Cuts at: 0 1051 1665
Size: 1051 614
BlpI GC'TnA_GC
Cuts at: 0 1062 1665
Size: 1062 603
Bme1580I G_kGCm'C
Cuts at: 0 611 741 1246 1277 1665
Size: 611 130 505 31 388
Fragments arranged by size:
611 505 388 130 31
BmgBI CAC'GTC
Cuts at: 0 606 1247 1512 1665
Size: 606 641 265 153
Fragments arranged by size:
641 606 265 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 461 1665
Size: 461 1204
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1599 1665
Size: 1599 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 490 925 1025 1665
Size: 358 132 435 100 640
Fragments arranged by size:
640 435 358 132 100
Bpu10I CC'TnA_GC
Cuts at: 0 1482 1665
Size: 1482 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 1665
Size: 574 1091
BsaI GGTCTCn'nnnn_
Cuts at: 0 1628 1665
Size: 1628 37
BsaWI w'CCGG_w
Cuts at: 0 1568 1665
Size: 1568 97
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1479 1509 1516 1546 1665
Size: 1479 30 7 30 119
Fragments arranged by size:
1479 119 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1270 1665
Size: 1270 395
BseYI C'CCAG_C
Cuts at: 0 1595 1665
Size: 1595 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 1665
Size: 183 1482
BsiEI CG_ry'CG
Cuts at: 0 1569 1665
Size: 1569 96
BsiHKAI G_wGCw'C
Cuts at: 0 335 1665
Size: 335 1330
BsiWI C'GTAC_G
Cuts at: 0 107 1665
Size: 107 1558
BsmI GAATG_Cn'
Cuts at: 0 348 1267 1665
Size: 348 919 398
Fragments arranged by size:
919 398 348
BsmBI CGTCTCn'nnnn_
Cuts at: 0 596 1665
Size: 596 1069
Bsp1286I G_dGCh'C
Cuts at: 0 335 498 611 741 1246 1277 1319 1665
Size: 335 163 113 130 505 31 42 346
Fragments arranged by size:
505 346 335 163 130 113 42 31
BspHI T'CATG_A
Cuts at: 0 743 818 1304 1665
Size: 743 75 486 361
Fragments arranged by size:
743 486 361 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 984 1044 1639 1665
Size: 984 60 595 26
Fragments arranged by size:
984 595 60 26
BsrBI CCG'CTC
Cuts at: 0 1061 1665
Size: 1061 604
BsrDI GCAATG_nn'
Cuts at: 0 644 887 1446 1665
Size: 644 243 559 219
Fragments arranged by size:
644 559 243 219
BsrFI r'CCGG_y
Cuts at: 0 1145 1665
Size: 1145 520
BsrGI T'GTAC_A
Cuts at: 0 392 1665
Size: 392 1273
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1499 1665
Size: 1499 166
BstEII G'GTnAC_C
Cuts at: 0 630 1665
Size: 630 1035
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 1665
Size: 210 1455
BstYI r'GATC_y
Cuts at: 0 1051 1560 1665
Size: 1051 509 105
Fragments arranged by size:
1051 509 105
Bsu36I CC'TnA_GG
Cuts at: 0 182 1106 1665
Size: 182 924 559
Fragments arranged by size:
924 559 182
BtgI C'CryG_G
Cuts at: 0 1520 1665
Size: 1520 145
BtsI GCAGTG_nn'
Cuts at: 0 1500 1562 1645 1665
Size: 1500 62 83 20
Fragments arranged by size:
1500 83 62 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1509 1544 1665
Size: 1509 35 121
Fragments arranged by size:
1509 121 35
DraIII CAC_nnn'GTG
Cuts at: 0 860 1665
Size: 860 805
DrdI GACnn_nn'nnGTC
Cuts at: 0 1150 1665
Size: 1150 515
EaeI y'GGCC_r
Cuts at: 0 1602 1665
Size: 1602 63
EarI CTCTTCn'nnn_
Cuts at: 0 257 430 1590 1665
Size: 257 173 1160 75
Fragments arranged by size:
1160 257 173 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 88 148 1665
Size: 82 6 60 1517
Fragments arranged by size:
1517 82 60 6
EcoICRI GAG'CTC
Cuts at: 0 333 1665
Size: 333 1332
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 490 528 640 925 980 1025 1665
Size: 358 132 38 112 285 55 45 640
Fragments arranged by size:
640 358 285 132 112 55 45 38
EcoO109I rG'GnC_Cy
Cuts at: 0 420 656 791 1094 1548 1665
Size: 420 236 135 303 454 117
Fragments arranged by size:
454 420 303 236 135 117
EcoRI G'AATT_C
Cuts at: 0 402 1665
Size: 402 1263
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 846 1665
Size: 846 819
FspI TGC'GCA
Cuts at: 0 283 1665
Size: 283 1382
FspAI rTGC'GCAy
Cuts at: 0 283 1665
Size: 283 1382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 516 548 1320 1352 1380 1412 1665
Size: 516 32 772 32 28 32 253
Fragments arranged by size:
772 516 253 32 32 32 28
HindIII A'AGCT_T
Cuts at: 0 1137 1665
Size: 1137 528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1274 1665
Size: 1274 391
MscI TGG'CCA
Cuts at: 0 1604 1665
Size: 1604 61
MslI CAynn'nnrTG
Cuts at: 0 208 292 545 748 994 1665
Size: 208 84 253 203 246 671
Fragments arranged by size:
671 253 246 208 203 84
MspA1I CmG'CkG
Cuts at: 0 522 1240 1665
Size: 522 718 425
Fragments arranged by size:
718 522 425
NspI r_CATG'y
Cuts at: 0 197 390 399 978 993 1665
Size: 197 193 9 579 15 672
Fragments arranged by size:
672 579 197 193 15 9
PasI CC'CwG_GG
Cuts at: 0 724 1665
Size: 724 941
PfoI T'CCnGG_A
Cuts at: 0 335 416 503 1454 1665
Size: 335 81 87 951 211
Fragments arranged by size:
951 335 211 87 81
PpuMI rG'GwC_Cy
Cuts at: 0 420 656 1665
Size: 420 236 1009
Fragments arranged by size:
1009 420 236
PshAI GACnn'nnGTC
Cuts at: 0 661 1514 1665
Size: 661 853 151
Fragments arranged by size:
853 661 151
PstI C_TGCA'G
Cuts at: 0 1131 1593 1652 1665
Size: 1131 462 59 13
Fragments arranged by size:
1131 462 59 13
PvuII CAG'CTG
Cuts at: 0 522 1665
Size: 522 1143
SacI G_AGCT'C
Cuts at: 0 335 1665
Size: 335 1330
SfcI C'TryA_G
Cuts at: 0 1127 1589 1648 1665
Size: 1127 462 59 17
Fragments arranged by size:
1127 462 59 17
SmlI C'TyrA_G
Cuts at: 0 168 589 1665
Size: 168 421 1076
Fragments arranged by size:
1076 421 168
SphI G_CATG'C
Cuts at: 0 390 993 1665
Size: 390 603 672
Fragments arranged by size:
672 603 390
StyI C'CwwG_G
Cuts at: 0 116 707 1665
Size: 116 591 958
Fragments arranged by size:
958 591 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 918 1487 1665
Size: 918 569 178
Fragments arranged by size:
918 569 178
TatI w'GTAC_w
Cuts at: 0 392 755 1665
Size: 392 363 910
Fragments arranged by size:
910 392 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1479 1498 1511 1530 1665
Size: 1479 19 13 19 135
Fragments arranged by size:
1479 135 19 19 13
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AlfI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BciVI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI FspAI Hin4I HindIII MmeI
MscI MslI MspA1I NspI PasI PfoI PpuMI PshAI
PstI PvuII SacI SfcI SmlI SphI StyI TaqII
TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BclI BfrBI BglI BmtI BsaAI BsaBI
BsaHI BspEI BssHII BssSI BstBI BstZ17I BtgZI ClaI
DraI EagI EcoNI EcoRV FseI HaeII HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI