Restriction map for
pFN21AE6765
(Linear) (Six-base) MAPSORT of: KIEE6765.seq Check: 8780 from: 1 to: 3057
KIEE6765.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AatII G_ACGT'C
Cuts at: 0 615 3057
Size: 615 2442
AccI GT'mk_AC
Cuts at: 0 561 2186 3057
Size: 561 1625 871
Fragments arranged by size:
1625 871 561
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 909 1572 1874 2110 2305 3057
Size: 909 663 302 236 195 752
Fragments arranged by size:
909 752 663 302 236 195
AflIII A'CryG_T
Cuts at: 0 266 3057
Size: 266 2791
AleI CACnn'nnGTG
Cuts at: 0 2710 3057
Size: 2710 347
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2267 3057
Size: 2267 790
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2929 2961 3057
Size: 2929 32 96
Fragments arranged by size:
2929 96 32
AlwNI CAG_nnn'CTG
Cuts at: 0 91 2457 3057
Size: 91 2366 600
Fragments arranged by size:
2366 600 91
ApaI G_GGCC'C
Cuts at: 0 1556 2919 3057
Size: 1556 1363 138
Fragments arranged by size:
1556 1363 138
ApoI r'AATT_y
Cuts at: 0 685 982 2010 2101 3057
Size: 685 297 1028 91 956
Fragments arranged by size:
1028 956 685 297 91
AvaI C'yCGr_G
Cuts at: 0 555 1518 3057
Size: 555 963 1539
Fragments arranged by size:
1539 963 555
BamHI G'GATC_C
Cuts at: 0 2693 2741 3057
Size: 2693 48 316
Fragments arranged by size:
2693 316 48
BanI G'GyrC_C
Cuts at: 0 452 816 1056 1780 1902 1997 3057
Size: 452 364 240 724 122 95 1060
Fragments arranged by size:
1060 724 452 364 240 122 95
BanII G_rGCy'C
Cuts at: 0 302 419 544 1046 1143 1556 2360 2919
Size: 302 117 125 502 97 413 804 559
Cuts at: 2919 2942 3057
Size: 23 115
Fragments arranged by size:
804 559 502 413 302 125 117 115
97 23
BbeI G_GCGC'C
Cuts at: 0 2001 3057
Size: 2001 1056
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 2487 2511 2562 3057
Size: 584 1903 24 51 495
Fragments arranged by size:
1903 584 495 51 24
BciVI GTATCCnnnnn_n'
Cuts at: 0 153 2554 3019 3057
Size: 153 2401 465 38
Fragments arranged by size:
2401 465 153 38
BfrBI ATG'CAT
Cuts at: 0 1251 3057
Size: 1251 1806
BglI GCCn_nnn'nGGC
Cuts at: 0 1900 2133 3057
Size: 1900 233 924
Fragments arranged by size:
1900 924 233
BglII A'GATC_T
Cuts at: 0 960 3031 3057
Size: 960 2071 26
Fragments arranged by size:
2071 960 26
BlpI GC'TnA_GC
Cuts at: 0 92 1966 3057
Size: 92 1874 1091
Fragments arranged by size:
1874 1091 92
Bme1580I G_kGCm'C
Cuts at: 0 1061 1486 1556 2436 2919 3057
Size: 1061 425 70 880 483 138
Fragments arranged by size:
1061 880 483 425 138 70
BmgBI CAC'GTC
Cuts at: 0 933 3057
Size: 933 2124
BmrI ACTGGGnnnn_n'
Cuts at: 0 859 2445 2937 3057
Size: 859 1586 492 120
Fragments arranged by size:
1586 859 492 120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1743 2590 3057
Size: 1743 847 467
Fragments arranged by size:
1743 847 467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 273 1034 1166 3057
Size: 85 188 761 132 1891
Fragments arranged by size:
1891 761 188 132 85
Bpu10I CC'TnA_GC
Cuts at: 0 489 3057
Size: 489 2568
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2395 3057
Size: 2395 662
BsaI GGTCTCn'nnnn_
Cuts at: 0 173 406 2419 3057
Size: 173 233 2013 638
Fragments arranged by size:
2013 638 233 173
BsaAI yAC'GTr
Cuts at: 0 267 2370 2679 3057
Size: 267 2103 309 378
Fragments arranged by size:
2103 378 309 267
BsaHI Gr'CG_yC
Cuts at: 0 612 1998 3057
Size: 612 1386 1059
Fragments arranged by size:
1386 1059 612
BsaWI w'CCGG_w
Cuts at: 0 66 1034 2267 3057
Size: 66 968 1233 790
Fragments arranged by size:
1233 968 790 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 531 561 2929 2959 3057
Size: 334 30 167 30 2368 30 98
Fragments arranged by size:
2368 334 167 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 72 462 818 1142 1163 1567 1732
Size: 28 44 390 356 324 21 404 165
Cuts at: 1732 2942 2950 3057
Size: 1210 8 107
Fragments arranged by size:
1210 404 390 356 324 165 107 44
28 21 8
BseYI C'CCAG_C
Cuts at: 0 22 84 798 1101 2190 2831 3057
Size: 22 62 714 303 1089 641 226
Fragments arranged by size:
1089 714 641 303 226 62 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 2734 3057
Size: 268 2466 323
Fragments arranged by size:
2466 323 268
BsiHKAI G_wGCw'C
Cuts at: 0 302 419 731 1143 2347 2738 3057
Size: 302 117 312 412 1204 391 319
Fragments arranged by size:
1204 412 391 319 312 302 117
Bsp1286I G_dGCh'C
Cuts at: 0 302 419 544 731 1046 1061 1143 1486
Size: 302 117 125 187 315 15 82 343
Cuts at: 1486 1556 2347 2360 2436 2738 2919 2942 3057
Size: 70 791 13 76 302 181 23 115
Fragments arranged by size:
791 343 315 302 302 187 181 125
117 115 82 76 70 23 15 13
BspEI T'CCGG_A
Cuts at: 0 66 1034 3057
Size: 66 968 2023
Fragments arranged by size:
2023 968 66
BspHI T'CATG_A
Cuts at: 0 689 2780 3057
Size: 689 2091 277
Fragments arranged by size:
2091 689 277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 316 590 3057
Size: 316 274 2467
Fragments arranged by size:
2467 316 274
BsrBI CCG'CTC
Cuts at: 0 485 3057
Size: 485 2572
BsrDI GCAATG_nn'
Cuts at: 0 896 1161 1605 3017 3057
Size: 896 265 444 1412 40
Fragments arranged by size:
1412 896 444 265 40
BsrFI r'CCGG_y
Cuts at: 0 1207 2296 2482 2553 3057
Size: 1207 1089 186 71 504
Fragments arranged by size:
1207 1089 504 186 71
BsrGI T'GTAC_A
Cuts at: 0 1925 3057
Size: 1925 1132
BssSI C'ACGA_G
Cuts at: 0 730 2920 3057
Size: 730 2190 137
Fragments arranged by size:
2190 730 137
BstBI TT'CG_AA
Cuts at: 0 2805 3057
Size: 2805 252
BstEII G'GTnAC_C
Cuts at: 0 2217 3057
Size: 2217 840
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1114 3057
Size: 1114 1943
BstYI r'GATC_y
Cuts at: 0 698 960 2693 2717 2741 2994 3031 3057
Size: 698 262 1733 24 24 253 37 26
Fragments arranged by size:
1733 698 262 253 37 26 24 24
BstZ17I GTA'TAC
Cuts at: 0 2187 3057
Size: 2187 870
Bsu36I CC'TnA_GG
Cuts at: 0 445 3057
Size: 445 2612
BtgI C'CryG_G
Cuts at: 0 572 1490 2176 2378 2753 2975 3057
Size: 572 918 686 202 375 222 82
Fragments arranged by size:
918 686 572 375 222 202 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 750 2376 3057
Size: 750 1626 681
Fragments arranged by size:
1626 750 681
BtsI GCAGTG_nn'
Cuts at: 0 1185 1203 1484 2249 3012 3057
Size: 1185 18 281 765 763 45
Fragments arranged by size:
1185 765 763 281 45 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1143 1178 3057
Size: 1143 35 1879
Fragments arranged by size:
1879 1143 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 617 3057
Size: 617 2440
EaeI y'GGCC_r
Cuts at: 0 409 791 888 1670 3057
Size: 409 382 97 782 1387
Fragments arranged by size:
1387 782 409 382 97
EarI CTCTTCn'nnn_
Cuts at: 0 298 928 1519 1585 2635 2707 2890 2896
Size: 298 630 591 66 1050 72 183 6
Cuts at: 2896 3057
Size: 161
Fragments arranged by size:
1050 630 591 298 183 161 72 66
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1151 1226 1612 3057
Size: 1151 75 386 1445
Fragments arranged by size:
1445 1151 386 75
EcoICRI GAG'CTC
Cuts at: 0 300 417 1141 3057
Size: 300 117 724 1916
Fragments arranged by size:
1916 724 300 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 273 909 1034 1166 1572 1874 2110
Size: 85 188 636 125 132 406 302 236
Cuts at: 2110 2305 3057
Size: 195 752
Fragments arranged by size:
752 636 406 302 236 195 188 132
125 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 1148 2141 3057
Size: 115 1033 993 916
Fragments arranged by size:
1033 993 916 115
EcoO109I rG'GnC_Cy
Cuts at: 0 80 853 1023 1552 2915 3057
Size: 80 773 170 529 1363 142
Fragments arranged by size:
1363 773 529 170 142 80
EcoRI G'AATT_C
Cuts at: 0 2010 3057
Size: 2010 1047
EcoRV GAT'ATC
Cuts at: 0 1311 3057
Size: 1311 1746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1596 1839 3057
Size: 1596 243 1218
Fragments arranged by size:
1596 1218 243
HaeII r_GCGC'y
Cuts at: 0 2001 2041 3057
Size: 2001 40 1016
Fragments arranged by size:
2001 1016 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 147 179 334 366 1514 1546 3057
Size: 147 32 155 32 1148 32 1511
Fragments arranged by size:
1511 1148 155 147 32 32 32
HincII GTy'rAC
Cuts at: 0 2265 3057
Size: 2265 792
KasI G'GCGC_C
Cuts at: 0 1997 3057
Size: 1997 1060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1931 3022 3057
Size: 746 1185 1091 35
Fragments arranged by size:
1185 1091 746 35
MscI TGG'CCA
Cuts at: 0 1672 3057
Size: 1672 1385
MslI CAynn'nnrTG
Cuts at: 0 1489 2332 2584 2710 3057
Size: 1489 843 252 126 347
Fragments arranged by size:
1489 843 347 252 126
MspA1I CmG'CkG
Cuts at: 0 88 675 1223 1648 2685 3057
Size: 88 587 548 425 1037 372
Fragments arranged by size:
1037 587 548 425 372 88
NaeI GCC'GGC
Cuts at: 0 1209 2298 3057
Size: 1209 1089 759
Fragments arranged by size:
1209 1089 759
NarI GG'CG_CC
Cuts at: 0 1998 3057
Size: 1998 1059
NcoI C'CATG_G
Cuts at: 0 1490 2975 3057
Size: 1490 1485 82
Fragments arranged by size:
1490 1485 82
NgoMIV G'CCGG_C
Cuts at: 0 1207 2296 3057
Size: 1207 1089 761
Fragments arranged by size:
1207 1089 761
NsiI A_TGCA'T
Cuts at: 0 1253 3057
Size: 1253 1804
PasI CC'CwG_GG
Cuts at: 0 2437 3057
Size: 2437 620
PflMI CCAn_nnn'nTGG
Cuts at: 0 2457 3057
Size: 2457 600
PfoI T'CCnGG_A
Cuts at: 0 101 419 1011 1143 3057
Size: 101 318 592 132 1914
Fragments arranged by size:
1914 592 318 132 101
PmlI CAC'GTG
Cuts at: 0 267 2679 3057
Size: 267 2412 378
Fragments arranged by size:
2412 378 267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2929 2961 3057
Size: 2929 32 96
Fragments arranged by size:
2929 96 32
PpuMI rG'GwC_Cy
Cuts at: 0 80 853 1023 3057
Size: 80 773 170 2034
Fragments arranged by size:
2034 773 170 80
PsiI TTA'TAA
Cuts at: 0 2965 3057
Size: 2965 92
PspOMI G'GGCC_C
Cuts at: 0 1552 2915 3057
Size: 1552 1363 142
Fragments arranged by size:
1552 1363 142
PstI C_TGCA'G
Cuts at: 0 170 317 3057
Size: 170 147 2740
Fragments arranged by size:
2740 170 147
PvuII CAG'CTG
Cuts at: 0 88 1648 3057
Size: 88 1560 1409
Fragments arranged by size:
1560 1409 88
SacI G_AGCT'C
Cuts at: 0 302 419 1143 3057
Size: 302 117 724 1914
Fragments arranged by size:
1914 724 302 117
SanDI GG'GwC_CC
Cuts at: 0 80 1023 3057
Size: 80 943 2034
Fragments arranged by size:
2034 943 80
SapI GCTCTTCn'nnn_
Cuts at: 0 2635 3057
Size: 2635 422
ScaI AGT'ACT
Cuts at: 0 1792 3057
Size: 1792 1265
SfcI C'TryA_G
Cuts at: 0 166 313 3057
Size: 166 147 2744
Fragments arranged by size:
2744 166 147
SfoI GGC'GCC
Cuts at: 0 1999 3057
Size: 1999 1058
SmlI C'TyrA_G
Cuts at: 0 2410 3057
Size: 2410 647
StyI C'CwwG_G
Cuts at: 0 704 1490 2975 3057
Size: 704 786 1485 82
Fragments arranged by size:
1485 786 704 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 2156 2310 3057
Size: 826 1330 154 747
Fragments arranged by size:
1330 826 747 154
TatI w'GTAC_w
Cuts at: 0 1790 1925 3057
Size: 1790 135 1132
Fragments arranged by size:
1790 1132 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 531 563 1351 1383 3057
Size: 531 32 788 32 1674
Fragments arranged by size:
1674 788 531 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 729 3057
Size: 729 2328
XmnI GAAnn'nnTTC
Cuts at: 0 2095 3057
Size: 2095 962
ZraI GAC'GTC
Cuts at: 0 613 3057
Size: 613 2444
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
PasI PflMI PfoI PmlI PpiI PpuMI PsiI PspOMI
PstI PvuII SacI SanDI SapI ScaI SfcI SfoI
SmlI StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI ApaLI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BclI
BmtI BsaBI BsiEI BsiWI BsmI BsmBI BssHII BstAPI
ClaI DraI DraIII EagI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NspI PacI PciI PmeI PshAI PspXI PsrI PvuI
RsrII SacII SalI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI