Restriction map for
pFN21AE6764
(Linear) (Six-base) MAPSORT of: KIEE6764.seq Check: 4933 from: 1 to: 2850
KIEE6764.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
Acc65I G'GTAC_C
Cuts at: 0 1006 2850
Size: 1006 1844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 2203 2850
Size: 269 1934 647
Fragments arranged by size:
1934 647 269
AflIII A'CryG_T
Cuts at: 0 1094 1591 2850
Size: 1094 497 1259
Fragments arranged by size:
1259 1094 497
AhdI GACnn_n'nnGTC
Cuts at: 0 2455 2850
Size: 2455 395
AleI CACnn'nnGTG
Cuts at: 0 2293 2754 2850
Size: 2293 461 96
Fragments arranged by size:
2293 461 96
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2221 2284 2590 2850
Size: 2221 63 306 260
Fragments arranged by size:
2221 306 260 63
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 1975 2007 2850
Size: 202 32 1741 32 843
Fragments arranged by size:
1741 843 202 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 960 2702 2823 2850
Size: 960 1742 121 27
Fragments arranged by size:
1742 960 121 27
ApaI G_GGCC'C
Cuts at: 0 561 836 2850
Size: 561 275 2014
Fragments arranged by size:
2014 561 275
ApaLI G'TGCA_C
Cuts at: 0 1407 1626 2850
Size: 1407 219 1224
Fragments arranged by size:
1407 1224 219
AscI GG'CGCG_CC
Cuts at: 0 624 2850
Size: 624 2226
AvaI C'yCGr_G
Cuts at: 0 133 531 919 1300 1835 2375 2411 2444
Size: 133 398 388 381 535 540 36 33
Cuts at: 2444 2786 2850
Size: 342 64
Fragments arranged by size:
540 535 398 388 381 342 133 64
36 33
BamHI G'GATC_C
Cuts at: 0 1566 2850
Size: 1566 1284
BanI G'GyrC_C
Cuts at: 0 391 1006 1315 1468 1809 1893 2113 2850
Size: 391 615 309 153 341 84 220 737
Fragments arranged by size:
737 615 391 341 309 220 153 84
BanII G_rGCy'C
Cuts at: 0 272 283 368 561 836 1366 1706 1993
Size: 272 11 85 193 275 530 340 287
Cuts at: 1993 2605 2678 2696 2850
Size: 612 73 18 154
Fragments arranged by size:
612 530 340 287 275 272 193 154
85 73 18 11
BbeI G_GCGC'C
Cuts at: 0 395 1319 2117 2850
Size: 395 924 798 733
Fragments arranged by size:
924 798 733 395
BbvCI CC'TCA_GC
Cuts at: 0 2476 2850
Size: 2476 374
BfrBI ATG'CAT
Cuts at: 0 2079 2850
Size: 2079 771
BglI GCCn_nnn'nGGC
Cuts at: 0 2771 2850
Size: 2771 79
BlpI GC'TnA_GC
Cuts at: 0 1716 2695 2850
Size: 1716 979 155
Fragments arranged by size:
1716 979 155
Bme1580I G_kGCm'C
Cuts at: 0 561 836 1256 1411 1630 1814 2290 2850
Size: 561 275 420 155 219 184 476 560
Fragments arranged by size:
561 560 476 420 275 219 184 155
BmgBI CAC'GTC
Cuts at: 0 318 2850
Size: 318 2532
BmrI ACTGGGnnnn_n'
Cuts at: 0 936 2850
Size: 936 1914
BmtI G_CTAG'C
Cuts at: 0 428 2850
Size: 428 2422
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 351 2367 2850
Size: 351 2016 483
Fragments arranged by size:
2016 483 351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 247 454 1720 2347 2850
Size: 68 179 207 1266 627 503
Fragments arranged by size:
1266 627 503 207 179 68
Bpu10I CC'TnA_GC
Cuts at: 0 1330 2476 2850
Size: 1330 1146 374
Fragments arranged by size:
1330 1146 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2103 2737 2850
Size: 2103 634 113
Fragments arranged by size:
2103 634 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 497 2850
Size: 497 2353
BsaAI yAC'GTr
Cuts at: 0 657 1095 2850
Size: 657 438 1755
Fragments arranged by size:
1755 657 438
BsaHI Gr'CG_yC
Cuts at: 0 205 254 392 1316 2114 2850
Size: 205 49 138 924 798 736
Fragments arranged by size:
924 798 736 205 138 49
BsaWI w'CCGG_w
Cuts at: 0 499 1723 2850
Size: 499 1224 1127
Fragments arranged by size:
1224 1127 499
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 452 482 1661 1691 1732 1762 1921 1951
Size: 452 30 1179 30 41 30 159 30
Cuts at: 1951 1975 2005 2407 2437 2850
Size: 24 30 402 30 413
Fragments arranged by size:
1179 452 413 402 159 41 30 30
30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1147 1756 2850
Size: 1147 609 1094
Fragments arranged by size:
1147 1094 609
BseYI C'CCAG_C
Cuts at: 0 9 766 1086 1279 1711 2525 2540 2850
Size: 9 757 320 193 432 814 15 310
Fragments arranged by size:
814 757 432 320 310 193 15 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1377 1627 2504 2850
Size: 1377 250 877 346
Fragments arranged by size:
1377 877 346 250
BsiEI CG_ry'CG
Cuts at: 0 111 1194 2730 2850
Size: 111 1083 1536 120
Fragments arranged by size:
1536 1083 120 111
BsiHKAI G_wGCw'C
Cuts at: 0 1411 1630 1924 2554 2696 2850
Size: 1411 219 294 630 142 154
Fragments arranged by size:
1411 630 294 219 154 142
BsmBI CGTCTCn'nnnn_
Cuts at: 0 655 2850
Size: 655 2195
Bsp1286I G_dGCh'C
Cuts at: 0 272 283 368 561 836 1256 1366 1411
Size: 272 11 85 193 275 420 110 45
Cuts at: 1411 1630 1706 1814 1924 1993 2290 2554 2605
Size: 219 76 108 110 69 297 264 51
Cuts at: 2605 2678 2696 2850
Size: 73 18 154
Fragments arranged by size:
420 297 275 272 264 219 193 154
110 110 108 85 76 73 69 51
45 18 11
BspEI T'CCGG_A
Cuts at: 0 499 1723 2850
Size: 499 1224 1127
Fragments arranged by size:
1224 1127 499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 605 2213 2850
Size: 299 306 1608 637
Fragments arranged by size:
1608 637 306 299
BsrBI CCG'CTC
Cuts at: 0 66 2241 2850
Size: 66 2175 609
Fragments arranged by size:
2175 609 66
BsrDI GCAATG_nn'
Cuts at: 0 855 2850
Size: 855 1995
BsrFI r'CCGG_y
Cuts at: 0 432 608 844 1784 2774 2850
Size: 432 176 236 940 990 76
Fragments arranged by size:
990 940 432 236 176 76
BssHII G'CGCG_C
Cuts at: 0 428 624 2850
Size: 428 196 2226
Fragments arranged by size:
2226 428 196
BssSI C'ACGA_G
Cuts at: 0 1081 2850
Size: 1081 1769
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1493 1661 1919 2850
Size: 1493 168 258 931
Fragments arranged by size:
1493 931 258 168
BstEII G'GTnAC_C
Cuts at: 0 840 2705 2850
Size: 840 1865 145
Fragments arranged by size:
1865 840 145
BstXI CCAn_nnnn'nTGG
Cuts at: 0 90 1905 2493 2850
Size: 90 1815 588 357
Fragments arranged by size:
1815 588 357 90
BstYI r'GATC_y
Cuts at: 0 1305 1566 2850
Size: 1305 261 1284
Fragments arranged by size:
1305 1284 261
Bsu36I CC'TnA_GG
Cuts at: 0 2839 2850
Size: 2839 11
BtgI C'CryG_G
Cuts at: 0 369 577 649 699 1799 2339 2354 2651
Size: 369 208 72 50 1100 540 15 297
Cuts at: 2651 2766 2850
Size: 115 84
Fragments arranged by size:
1100 540 369 297 208 115 84 72
50 15
BtsI GCAGTG_nn'
Cuts at: 0 932 1073 1224 1345 1630 1753 2574 2850
Size: 932 141 151 121 285 123 821 276
Fragments arranged by size:
932 821 285 276 151 141 123 121
DraIII CAC_nnn'GTG
Cuts at: 0 1542 1919 2850
Size: 1542 377 931
Fragments arranged by size:
1542 931 377
DrdI GACnn_nn'nnGTC
Cuts at: 0 2833 2850
Size: 2833 17
EaeI y'GGCC_r
Cuts at: 0 108 1613 2099 2727 2850
Size: 108 1505 486 628 123
Fragments arranged by size:
1505 628 486 123 108
EagI C'GGCC_G
Cuts at: 0 108 2727 2850
Size: 108 2619 123
Fragments arranged by size:
2619 123 108
EarI CTCTTCn'nnn_
Cuts at: 0 288 949 1993 2068 2850
Size: 288 661 1044 75 782
Fragments arranged by size:
1044 782 661 288 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2122 2595 2850
Size: 2122 473 255
Fragments arranged by size:
2122 473 255
EcoICRI GAG'CTC
Cuts at: 0 2694 2850
Size: 2694 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 247 269 454 1720 2203 2347 2850
Size: 68 179 22 185 1266 483 144 503
Fragments arranged by size:
1266 503 483 185 179 144 68 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1300 2850
Size: 1300 1550
EcoO109I rG'GnC_Cy
Cuts at: 0 130 558 957 1505 2244 2346 2439 2449
Size: 130 428 399 548 739 102 93 10
Cuts at: 2449 2513 2850
Size: 64 337
Fragments arranged by size:
739 548 428 399 337 130 102 93
64 10
FspI TGC'GCA
Cuts at: 0 868 2850
Size: 868 1982
HaeII r_GCGC'y
Cuts at: 0 395 477 1319 2117 2702 2748 2850
Size: 395 82 842 798 585 46 102
Fragments arranged by size:
842 798 585 395 102 82 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 496 528 811 843 1511 1543 1921 1953
Size: 496 32 283 32 668 32 378 32
Cuts at: 1953 2407 2410 2439 2441 2442 2473 2850
Size: 454 3 29 2 1 31 377
Fragments arranged by size:
668 496 454 378 377 283 32 32
32 32 31 29 3 2 1
KasI G'GCGC_C
Cuts at: 0 391 1315 2113 2850
Size: 391 924 798 737
Fragments arranged by size:
924 798 737 391
KpnI G_GTAC'C
Cuts at: 0 1010 2850
Size: 1010 1840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 2510 2850
Size: 111 2399 340
Fragments arranged by size:
2399 340 111
MscI TGG'CCA
Cuts at: 0 1615 2101 2850
Size: 1615 486 749
Fragments arranged by size:
1615 749 486
MslI CAynn'nnrTG
Cuts at: 0 793 1115 2293 2754 2850
Size: 793 322 1178 461 96
Fragments arranged by size:
1178 793 461 322 96
MspA1I CmG'CkG
Cuts at: 0 46 371 414 579 651 1250 1475 1611
Size: 46 325 43 165 72 599 225 136
Cuts at: 1611 2279 2293 2334 2540 2850
Size: 668 14 41 206 310
Fragments arranged by size:
668 599 325 310 225 206 165 136
72 46 43 41 14
NaeI GCC'GGC
Cuts at: 0 434 610 2776 2850
Size: 434 176 2166 74
Fragments arranged by size:
2166 434 176 74
NarI GG'CG_CC
Cuts at: 0 392 1316 2114 2850
Size: 392 924 798 736
Fragments arranged by size:
924 798 736 392
NgoMIV G'CCGG_C
Cuts at: 0 432 608 2774 2850
Size: 432 176 2166 76
Fragments arranged by size:
2166 432 176 76
NheI G'CTAG_C
Cuts at: 0 424 2850
Size: 424 2426
NsiI A_TGCA'T
Cuts at: 0 2081 2850
Size: 2081 769
NspI r_CATG'y
Cuts at: 0 1107 1386 1595 2850
Size: 1107 279 209 1255
Fragments arranged by size:
1255 1107 279 209
PasI CC'CwG_GG
Cuts at: 0 987 1457 2850
Size: 987 470 1393
Fragments arranged by size:
1393 987 470
PciI A'CATG_T
Cuts at: 0 1591 2850
Size: 1591 1259
PflMI CCAn_nnn'nTGG
Cuts at: 0 1086 1205 1403 2823 2850
Size: 1086 119 198 1420 27
Fragments arranged by size:
1420 1086 198 119 27
PfoI T'CCnGG_A
Cuts at: 0 1241 1268 1736 2324 2850
Size: 1241 27 468 588 526
Fragments arranged by size:
1241 588 526 468 27
PmlI CAC'GTG
Cuts at: 0 1095 2850
Size: 1095 1755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1975 2007 2850
Size: 1975 32 843
Fragments arranged by size:
1975 843 32
PpuMI rG'GwC_Cy
Cuts at: 0 130 957 2244 2346 2439 2449 2850
Size: 130 827 1287 102 93 10 401
Fragments arranged by size:
1287 827 401 130 102 93 10
PspOMI G'GGCC_C
Cuts at: 0 557 832 2850
Size: 557 275 2018
Fragments arranged by size:
2018 557 275
PstI C_TGCA'G
Cuts at: 0 1219 2309 2579 2850
Size: 1219 1090 270 271
Fragments arranged by size:
1219 1090 271 270
PvuII CAG'CTG
Cuts at: 0 1475 1611 2540 2850
Size: 1475 136 929 310
Fragments arranged by size:
1475 929 310 136
SacI G_AGCT'C
Cuts at: 0 2696 2850
Size: 2696 154
SacII CC_GC'GG
Cuts at: 0 372 580 652 2850
Size: 372 208 72 2198
Fragments arranged by size:
2198 372 208 72
SanDI GG'GwC_CC
Cuts at: 0 130 2449 2850
Size: 130 2319 401
Fragments arranged by size:
2319 401 130
SapI GCTCTTCn'nnn_
Cuts at: 0 288 2850
Size: 288 2562
SbfI CC_TGCA'GG
Cuts at: 0 2309 2850
Size: 2309 541
ScaI AGT'ACT
Cuts at: 0 925 1390 1660 1819 2850
Size: 925 465 270 159 1031
Fragments arranged by size:
1031 925 465 270 159
SexAI A'CCwGG_T
Cuts at: 0 2246 2489 2850
Size: 2246 243 361
Fragments arranged by size:
2246 361 243
SfcI C'TryA_G
Cuts at: 0 1215 2305 2575 2850
Size: 1215 1090 270 275
Fragments arranged by size:
1215 1090 275 270
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2771 2850
Size: 2771 79
SfoI GGC'GCC
Cuts at: 0 393 1317 2115 2850
Size: 393 924 798 735
Fragments arranged by size:
924 798 735 393
SgrAI Cr'CCGG_yG
Cuts at: 0 432 2850
Size: 432 2418
SmaI CCC'GGG
Cuts at: 0 1837 2377 2446 2788 2850
Size: 1837 540 69 342 62
Fragments arranged by size:
1837 540 342 69 62
SmlI C'TyrA_G
Cuts at: 0 2118 2752 2850
Size: 2118 634 98
Fragments arranged by size:
2118 634 98
StyI C'CwwG_G
Cuts at: 0 836 1421 1898 1950 2193 2435 2850
Size: 836 585 477 52 243 242 415
Fragments arranged by size:
836 585 477 415 243 242 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1797 1911 2517 2850
Size: 1797 114 606 333
Fragments arranged by size:
1797 606 333 114
TatI w'GTAC_w
Cuts at: 0 923 1388 1658 1817 2850
Size: 923 465 270 159 1033
Fragments arranged by size:
1033 923 465 270 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 1153 1185 1418 1450 1661 1693
Size: 452 32 669 32 233 32 211 32
Cuts at: 1693 1732 1764 2850
Size: 39 32 1086
Fragments arranged by size:
1086 669 452 233 211 39 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 1428 2850
Size: 246 1182 1422
Fragments arranged by size:
1422 1182 246
XmaI C'CCGG_G
Cuts at: 0 1835 2375 2444 2786 2850
Size: 1835 540 69 342 64
Fragments arranged by size:
1835 540 342 69 64
XmnI GAAnn'nnTTC
Cuts at: 0 2002 2850
Size: 2002 848
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AleI AlfI AloI AlwNI
ApaI ApaLI AscI AvaI BamHI BanI BanII BbeI
BbvCI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI SmlI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI ApoI
AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI
BglII BsaBI BsiWI BsmI BspHI BsrGI BstBI BstZ17I
BtgZI ClaI CspCI DraI EcoRI EcoRV FalI FseI
FspAI HincII HindIII HpaI MfeI MluI NcoI NdeI
NotI NruI PacI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SgfI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI ZraI