Restriction map for
pFN21AE6763
(Linear) (Six-base) MAPSORT of: KIEE6763.seq Check: 6846 from: 1 to: 2520
KIEE6763.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AatII G_ACGT'C
Cuts at: 0 947 1808 2156 2520
Size: 947 861 348 364
Fragments arranged by size:
947 861 364 348
AccI GT'mk_AC
Cuts at: 0 737 914 2520
Size: 737 177 1606
Fragments arranged by size:
1606 737 177
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1680 2284 2285 2520
Size: 412 1268 604 1 235
Fragments arranged by size:
1268 604 412 235 1
AflIII A'CryG_T
Cuts at: 0 587 929 997 1853 2520
Size: 587 342 68 856 667
Fragments arranged by size:
856 667 587 342 68
AlwNI CAG_nnn'CTG
Cuts at: 0 487 849 2520
Size: 487 362 1671
Fragments arranged by size:
1671 487 362
ApaI G_GGCC'C
Cuts at: 0 2238 2520
Size: 2238 282
ApoI r'AATT_y
Cuts at: 0 148 647 1111 2520
Size: 148 499 464 1409
Fragments arranged by size:
1409 499 464 148
AvaI C'yCGr_G
Cuts at: 0 241 2520
Size: 241 2279
BamHI G'GATC_C
Cuts at: 0 627 2024 2520
Size: 627 1397 496
Fragments arranged by size:
1397 627 496
BanII G_rGCy'C
Cuts at: 0 86 95 2118 2238 2520
Size: 86 9 2023 120 282
Fragments arranged by size:
2023 282 120 86 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 1312 2520
Size: 212 1100 1208
Fragments arranged by size:
1208 1100 212
BbvCI CC'TCA_GC
Cuts at: 0 1672 2239 2520
Size: 1672 567 281
Fragments arranged by size:
1672 567 281
BciVI GTATCCnnnnn_n'
Cuts at: 0 1756 2520
Size: 1756 764
BclI T'GATC_A
Cuts at: 0 527 1778 2381 2520
Size: 527 1251 603 139
Fragments arranged by size:
1251 603 527 139
BglI GCCn_nnn'nGGC
Cuts at: 0 2084 2520
Size: 2084 436
BglII A'GATC_T
Cuts at: 0 710 971 2520
Size: 710 261 1549
Fragments arranged by size:
1549 710 261
BlpI GC'TnA_GC
Cuts at: 0 80 2520
Size: 80 2440
Bme1580I G_kGCm'C
Cuts at: 0 1014 2238 2520
Size: 1014 1224 282
Fragments arranged by size:
1224 1014 282
BmrI ACTGGGnnnn_n'
Cuts at: 0 2436 2520
Size: 2436 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 706 2412 2520
Size: 706 1706 108
Fragments arranged by size:
1706 706 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 202 307 490 643 1036 2049 2520
Size: 34 168 105 183 153 393 1013 471
Fragments arranged by size:
1013 471 393 183 168 153 105 34
Bpu10I CC'TnA_GC
Cuts at: 0 691 1672 2239 2520
Size: 691 981 567 281
Fragments arranged by size:
981 691 567 281
BsaAI yAC'GTr
Cuts at: 0 1000 1812 2520
Size: 1000 812 708
Fragments arranged by size:
1000 812 708
BsaBI GATnn'nnATC
Cuts at: 0 1744 2520
Size: 1744 776
BsaHI Gr'CG_yC
Cuts at: 0 614 944 1805 2153 2520
Size: 614 330 861 348 367
Fragments arranged by size:
861 614 367 348 330
BsaWI w'CCGG_w
Cuts at: 0 499 2520
Size: 499 2021
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 32 62 862 892 1071 1101 1393 1423
Size: 32 30 800 30 179 30 292 30
Cuts at: 1423 2200 2230 2309 2339 2520
Size: 777 30 79 30 181
Fragments arranged by size:
800 777 292 181 179 79 32 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 1032 2520
Size: 37 995 1488
Fragments arranged by size:
1488 995 37
BseYI C'CCAG_C
Cuts at: 0 950 1176 1518 1828 2006 2312 2520
Size: 950 226 342 310 178 306 208
Fragments arranged by size:
950 342 310 306 226 208 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1247 2392 2407 2520
Size: 916 331 1145 15 113
Fragments arranged by size:
1145 916 331 113 15
BsiHKAI G_wGCw'C
Cuts at: 0 86 95 2273 2520
Size: 86 9 2178 247
Fragments arranged by size:
2178 247 86 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 409 1281 2144 2520
Size: 409 872 863 376
Fragments arranged by size:
872 863 409 376
Bsp1286I G_dGCh'C
Cuts at: 0 86 95 1014 2118 2238 2273 2520
Size: 86 9 919 1104 120 35 247
Fragments arranged by size:
1104 919 247 120 86 35 9
BspHI T'CATG_A
Cuts at: 0 1058 1775 2520
Size: 1058 717 745
Fragments arranged by size:
1058 745 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2231 2520
Size: 2231 289
BsrBI CCG'CTC
Cuts at: 0 264 558 2520
Size: 264 294 1962
Fragments arranged by size:
1962 294 264
BsrFI r'CCGG_y
Cuts at: 0 520 2520
Size: 520 2000
BstAPI GCAn_nnn'nTGC
Cuts at: 0 487 2415 2520
Size: 487 1928 105
Fragments arranged by size:
1928 487 105
BstBI TT'CG_AA
Cuts at: 0 15 2520
Size: 15 2505
BstYI r'GATC_y
Cuts at: 0 627 710 971 2024 2520
Size: 627 83 261 1053 496
Fragments arranged by size:
1053 627 496 261 83
BtgI C'CryG_G
Cuts at: 0 268 1046 1338 1954 2109 2127 2520
Size: 268 778 292 616 155 18 393
Fragments arranged by size:
778 616 393 292 268 155 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 948 2520
Size: 948 1572
BtsI GCAGTG_nn'
Cuts at: 0 488 961 2520
Size: 488 473 1559
Fragments arranged by size:
1559 488 473
DraI TTT'AAA
Cuts at: 0 119 2490 2520
Size: 119 2371 30
Fragments arranged by size:
2371 119 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 2158 2520
Size: 2158 362
EaeI y'GGCC_r
Cuts at: 0 2085 2520
Size: 2085 435
EarI CTCTTCn'nnn_
Cuts at: 0 1440 1884 2129 2520
Size: 1440 444 245 391
Fragments arranged by size:
1440 444 391 245
EcoICRI GAG'CTC
Cuts at: 0 84 93 2520
Size: 84 9 2427
Fragments arranged by size:
2427 84 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 202 307 412 490 643 1036 1680
Size: 34 168 105 105 78 153 393 644
Cuts at: 1680 2049 2284 2285 2520
Size: 369 235 1 235
Fragments arranged by size:
644 393 369 235 235 168 153 105
105 78 34 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 853 2520
Size: 853 1667
EcoO109I rG'GnC_Cy
Cuts at: 0 2235 2391 2520
Size: 2235 156 129
Fragments arranged by size:
2235 156 129
HaeII r_GCGC'y
Cuts at: 0 849 1347 2520
Size: 849 498 1173
Fragments arranged by size:
1173 849 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 719 751 1280 1312 1393 1425 2309 2341
Size: 719 32 529 32 81 32 884 32
Cuts at: 2341 2520
Size: 179
Fragments arranged by size:
884 719 529 179 81 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 357 2520
Size: 357 2163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1473 1907 2520
Size: 758 715 434 613
Fragments arranged by size:
758 715 613 434
MscI TGG'CCA
Cuts at: 0 2087 2520
Size: 2087 433
MslI CAynn'nnrTG
Cuts at: 0 892 1852 2520
Size: 892 960 668
Fragments arranged by size:
960 892 668
MspA1I CmG'CkG
Cuts at: 0 865 1120 1512 2010 2294 2520
Size: 865 255 392 498 284 226
Fragments arranged by size:
865 498 392 284 255 226
NcoI C'CATG_G
Cuts at: 0 1046 2127 2520
Size: 1046 1081 393
Fragments arranged by size:
1081 1046 393
NspI r_CATG'y
Cuts at: 0 933 1857 2520
Size: 933 924 663
Fragments arranged by size:
933 924 663
PciI A'CATG_T
Cuts at: 0 929 1853 2520
Size: 929 924 667
Fragments arranged by size:
929 924 667
PfoI T'CCnGG_A
Cuts at: 0 467 2020 2498 2520
Size: 467 1553 478 22
Fragments arranged by size:
1553 478 467 22
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 961 993 2520
Size: 961 32 1527
Fragments arranged by size:
1527 961 32
PshAI GACnn'nnGTC
Cuts at: 0 1235 2520
Size: 1235 1285
PspOMI G'GGCC_C
Cuts at: 0 2234 2520
Size: 2234 286
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 583 615 2520
Size: 583 32 1905
Fragments arranged by size:
1905 583 32
PstI C_TGCA'G
Cuts at: 0 857 1511 2015 2103 2520
Size: 857 654 504 88 417
Fragments arranged by size:
857 654 504 417 88
PvuII CAG'CTG
Cuts at: 0 1120 1512 2010 2520
Size: 1120 392 498 510
Fragments arranged by size:
1120 510 498 392
SacI G_AGCT'C
Cuts at: 0 86 95 2520
Size: 86 9 2425
Fragments arranged by size:
2425 86 9
SapI GCTCTTCn'nnn_
Cuts at: 0 1440 2520
Size: 1440 1080
ScaI AGT'ACT
Cuts at: 0 2188 2520
Size: 2188 332
SexAI A'CCwGG_T
Cuts at: 0 1766 2520
Size: 1766 754
SfcI C'TryA_G
Cuts at: 0 853 1507 1869 2011 2099 2520
Size: 853 654 362 142 88 421
Fragments arranged by size:
853 654 421 362 142 88
SmlI C'TyrA_G
Cuts at: 0 241 2520
Size: 241 2279
SspI AAT'ATT
Cuts at: 0 1134 2520
Size: 1134 1386
StuI AGG'CCT
Cuts at: 0 346 2520
Size: 346 2174
StyI C'CwwG_G
Cuts at: 0 678 1046 2127 2520
Size: 678 368 1081 393
Fragments arranged by size:
1081 678 393 368
SwaI ATTT'AAAT
Cuts at: 0 119 2520
Size: 119 2401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 964 2520
Size: 964 1556
TatI w'GTAC_w
Cuts at: 0 590 2186 2520
Size: 590 1596 334
Fragments arranged by size:
1596 590 334
XbaI T'CTAG_A
Cuts at: 0 297 2520
Size: 297 2223
XhoI C'TCGA_G
Cuts at: 0 241 2520
Size: 241 2279
XmnI GAAnn'nnTTC
Cuts at: 0 647 661 1330 2446 2520
Size: 647 14 669 1116 74
Fragments arranged by size:
1116 669 647 74 14
ZraI GAC'GTC
Cuts at: 0 945 1806 2154 2520
Size: 945 861 348 366
Fragments arranged by size:
945 861 366 348
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApaI ApoI AvaI
BamHI BanII BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII MmeI MscI
MslI MspA1I NcoI NspI PciI PfoI PpiI PshAI
PspOMI PsrI PstI PvuII SacI SapI ScaI SexAI
SfcI SmlI SspI StuI StyI SwaI TaqII TatI
XbaI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BcgI BfrBI BmgBI BmtI BpuEI BsaI
BsiEI BsiWI BsmI BspEI BsrDI BsrGI BssHII BssSI
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EagI
EciI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpuMI PsiI PspXI PvuI RsrII SacII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI TstI Tth111I XcmI XmaI