Restriction map for
pFN21AE6759
(Linear) (Six-base) MAPSORT of: KIEE6759.seq Check: 455 from: 1 to: 1629
KIEE6759.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1629
Size: 786 843
AhdI GACnn_n'nnGTC
Cuts at: 0 1002 1629
Size: 1002 627
AleI CACnn'nnGTG
Cuts at: 0 929 1629
Size: 929 700
AlwNI CAG_nnn'CTG
Cuts at: 0 114 318 1023 1629
Size: 114 204 705 606
Fragments arranged by size:
705 606 204 114
ApaI G_GGCC'C
Cuts at: 0 160 1629
Size: 160 1469
ApoI r'AATT_y
Cuts at: 0 778 1509 1602 1629
Size: 778 731 93 27
Fragments arranged by size:
778 731 93 27
AvaI C'yCGr_G
Cuts at: 0 153 496 576 655 853 1061 1629
Size: 153 343 80 79 198 208 568
Fragments arranged by size:
568 343 208 198 153 80 79
BanI G'GyrC_C
Cuts at: 0 562 985 1629
Size: 562 423 644
Fragments arranged by size:
644 562 423
BanII G_rGCy'C
Cuts at: 0 160 334 1583 1629
Size: 160 174 1249 46
Fragments arranged by size:
1249 174 160 46
BbeI G_GCGC'C
Cuts at: 0 566 989 1629
Size: 566 423 640
Fragments arranged by size:
640 566 423
BbvCI CC'TCA_GC
Cuts at: 0 13 118 1629
Size: 13 105 1511
Fragments arranged by size:
1511 105 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 381 415 1629
Size: 381 34 1214
Fragments arranged by size:
1214 381 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1055 1629
Size: 1055 574
BlpI GC'TnA_GC
Cuts at: 0 511 673 1629
Size: 511 162 956
Fragments arranged by size:
956 511 162
Bme1580I G_kGCm'C
Cuts at: 0 160 1629
Size: 160 1469
BmgBI CAC'GTC
Cuts at: 0 726 1629
Size: 726 903
BmrI ACTGGGnnnn_n'
Cuts at: 0 1285 1629
Size: 1285 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1158 1629
Size: 1158 471
Bpu10I CC'TnA_GC
Cuts at: 0 13 118 1629
Size: 13 105 1511
Fragments arranged by size:
1511 105 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 1629
Size: 849 780
BsaAI yAC'GTr
Cuts at: 0 507 1629
Size: 507 1122
BsaHI Gr'CG_yC
Cuts at: 0 563 881 986 1629
Size: 563 318 105 643
Fragments arranged by size:
643 563 318 105
BsaWI w'CCGG_w
Cuts at: 0 140 1629
Size: 140 1489
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1086 1116 1629
Size: 1086 30 513
Fragments arranged by size:
1086 513 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1018 1021 1629
Size: 1018 3 608
Fragments arranged by size:
1018 608 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 448 1075 1087 1629
Size: 343 105 627 12 542
Fragments arranged by size:
627 542 343 105 12
BsiEI CG_ry'CG
Cuts at: 0 348 352 387 914 1629
Size: 348 4 35 527 715
Fragments arranged by size:
715 527 348 35 4
BsiHKAI G_wGCw'C
Cuts at: 0 419 1583 1629
Size: 419 1164 46
Fragments arranged by size:
1164 419 46
Bsp1286I G_dGCh'C
Cuts at: 0 160 334 419 1583 1629
Size: 160 174 85 1164 46
Fragments arranged by size:
1164 174 160 85 46
BspHI T'CATG_A
Cuts at: 0 611 1284 1629
Size: 611 673 345
Fragments arranged by size:
673 611 345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1139 1629
Size: 1139 490
BsrFI r'CCGG_y
Cuts at: 0 347 351 960 1629
Size: 347 4 609 669
Fragments arranged by size:
669 609 347 4
BssHII G'CGCG_C
Cuts at: 0 300 435 520 935 1629
Size: 300 135 85 415 694
Fragments arranged by size:
694 415 300 135 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1023 1629
Size: 1023 606
BstEII G'GTnAC_C
Cuts at: 0 1117 1629
Size: 1117 512
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1298 1629
Size: 1298 331
BstYI r'GATC_y
Cuts at: 0 257 1629
Size: 257 1372
BtgI C'CryG_G
Cuts at: 0 599 1574 1629
Size: 599 975 55
Fragments arranged by size:
975 599 55
BtsI GCAGTG_nn'
Cuts at: 0 834 1024 1208 1629
Size: 834 190 184 421
Fragments arranged by size:
834 421 190 184
DraI TTT'AAA
Cuts at: 0 1548 1629
Size: 1548 81
EaeI y'GGCC_r
Cuts at: 0 31 89 169 349 384 402 596 602
Size: 31 58 80 180 35 18 194 6
Cuts at: 602 911 962 1629
Size: 309 51 667
Fragments arranged by size:
667 309 194 180 80 58 51 35
31 18 6
EagI C'GGCC_G
Cuts at: 0 349 384 911 1629
Size: 349 35 527 718
Fragments arranged by size:
718 527 349 35
EarI CTCTTCn'nnn_
Cuts at: 0 680 1039 1450 1629
Size: 680 359 411 179
Fragments arranged by size:
680 411 359 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 250 1629
Size: 250 1379
EcoICRI GAG'CTC
Cuts at: 0 1581 1629
Size: 1581 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1158 1629
Size: 786 372 471
Fragments arranged by size:
786 471 372
EcoO109I rG'GnC_Cy
Cuts at: 0 315 1362 1629
Size: 315 1047 267
Fragments arranged by size:
1047 315 267
EcoRI G'AATT_C
Cuts at: 0 778 1629
Size: 778 851
FseI GG_CCGG'CC
Cuts at: 0 355 1629
Size: 355 1274
FspI TGC'GCA
Cuts at: 0 457 763 1629
Size: 457 306 866
Fragments arranged by size:
866 457 306
FspAI rTGC'GCAy
Cuts at: 0 763 1629
Size: 763 866
HaeII r_GCGC'y
Cuts at: 0 240 538 566 989 1629
Size: 240 298 28 423 640
Fragments arranged by size:
640 423 298 240 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 988 1020 1086 1118 1629
Size: 988 32 66 32 511
Fragments arranged by size:
988 511 66 32 32
HincII GTy'rAC
Cuts at: 0 1266 1629
Size: 1266 363
KasI G'GCGC_C
Cuts at: 0 562 985 1629
Size: 562 423 644
Fragments arranged by size:
644 562 423
MscI TGG'CCA
Cuts at: 0 91 171 604 1629
Size: 91 80 433 1025
Fragments arranged by size:
1025 433 91 80
MslI CAynn'nnrTG
Cuts at: 0 283 929 1629
Size: 283 646 700
Fragments arranged by size:
700 646 283
MspA1I CmG'CkG
Cuts at: 0 45 111 272 432 585 1629
Size: 45 66 161 160 153 1044
Fragments arranged by size:
1044 161 160 153 66 45
NaeI GCC'GGC
Cuts at: 0 353 1629
Size: 353 1276
NarI GG'CG_CC
Cuts at: 0 563 986 1629
Size: 563 423 643
Fragments arranged by size:
643 563 423
NcoI C'CATG_G
Cuts at: 0 1574 1629
Size: 1574 55
NgoMIV G'CCGG_C
Cuts at: 0 351 1629
Size: 351 1278
NotI GC'GGCC_GC
Cuts at: 0 384 1629
Size: 384 1245
NspI r_CATG'y
Cuts at: 0 103 1629
Size: 103 1526
PflMI CCAn_nnn'nTGG
Cuts at: 0 318 1629
Size: 318 1311
PfoI T'CCnGG_A
Cuts at: 0 815 1629
Size: 815 814
PmlI CAC'GTG
Cuts at: 0 507 1629
Size: 507 1122
PpuMI rG'GwC_Cy
Cuts at: 0 1362 1629
Size: 1362 267
PspOMI G'GGCC_C
Cuts at: 0 156 1629
Size: 156 1473
PspXI vC'TCGA_Gb
Cuts at: 0 496 1061 1629
Size: 496 565 568
Fragments arranged by size:
568 565 496
PstI C_TGCA'G
Cuts at: 0 743 1019 1629
Size: 743 276 610
Fragments arranged by size:
743 610 276
PvuII CAG'CTG
Cuts at: 0 272 1629
Size: 272 1357
RsrII CG'GwC_CG
Cuts at: 0 143 1629
Size: 143 1486
SacI G_AGCT'C
Cuts at: 0 1583 1629
Size: 1583 46
ScaI AGT'ACT
Cuts at: 0 745 1629
Size: 745 884
SexAI A'CCwGG_T
Cuts at: 0 1039 1629
Size: 1039 590
SfcI C'TryA_G
Cuts at: 0 639 739 1015 1629
Size: 639 100 276 614
Fragments arranged by size:
639 614 276 100
SfoI GGC'GCC
Cuts at: 0 564 987 1629
Size: 564 423 642
Fragments arranged by size:
642 564 423
SmaI CCC'GGG
Cuts at: 0 155 855 1629
Size: 155 700 774
Fragments arranged by size:
774 700 155
SmlI C'TyrA_G
Cuts at: 0 496 828 1061 1629
Size: 496 332 233 568
Fragments arranged by size:
568 496 332 233
StyI C'CwwG_G
Cuts at: 0 28 1244 1574 1613 1629
Size: 28 1216 330 39 16
Fragments arranged by size:
1216 330 39 28 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1105 1629
Size: 1105 524
TatI w'GTAC_w
Cuts at: 0 743 1629
Size: 743 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1029 1061 1629
Size: 1029 32 568
Fragments arranged by size:
1029 568 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 99 1251 1629
Size: 99 1152 378
Fragments arranged by size:
1152 378 99
XhoI C'TCGA_G
Cuts at: 0 496 1061 1629
Size: 496 565 568
Fragments arranged by size:
568 565 496
XmaI C'CCGG_G
Cuts at: 0 153 853 1629
Size: 153 700 776
Fragments arranged by size:
776 700 153
XmnI GAAnn'nnTTC
Cuts at: 0 845 1629
Size: 845 784
Enzymes that do cut:
AcuI AhdI AleI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbvCI BcgI BciVI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI
BssHII BstAPI BstEII BstXI BstYI BtgI BtsI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FseI FspI FspAI HaeII Hin4I HincII KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII
RsrII SacI ScaI SexAI SfcI SfoI SmaI SmlI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BclI BfrBI BglI BglII BmtI
BplI BsaI BsaBI BseYI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EcoNI EcoRV FalI HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NruI
NsiI PacI PasI PciI PmeI PpiI PshAI PsiI
PsrI PvuI SacII SalI SanDI SapI SbfI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI ZraI