Restriction map for
pFN21AE6756
(Linear) (Six-base) MAPSORT of: KIEE6756.seq Check: 4931 from: 1 to: 2436
KIEE6756.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 1319 1360 2198 2436
Size: 707 612 41 838 238
Fragments arranged by size:
838 707 612 238 41
AflII C'TTAA_G
Cuts at: 0 1273 2436
Size: 1273 1163
AflIII A'CryG_T
Cuts at: 0 1693 2436
Size: 1693 743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1646 2436
Size: 1646 790
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 765 797 2436
Size: 765 32 1639
Fragments arranged by size:
1639 765 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1823 2436
Size: 1823 613
ApoI r'AATT_y
Cuts at: 0 202 704 863 950 1076 1704 2189 2436
Size: 202 502 159 87 126 628 485 247
Fragments arranged by size:
628 502 485 247 202 159 126 87
BanI G'GyrC_C
Cuts at: 0 2022 2436
Size: 2022 414
BanII G_rGCy'C
Cuts at: 0 234 2172 2384 2436
Size: 234 1938 212 52
Fragments arranged by size:
1938 234 212 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 673 2436
Size: 673 1763
BbvCI CC'TCA_GC
Cuts at: 0 650 2436
Size: 650 1786
BfrBI ATG'CAT
Cuts at: 0 1432 2436
Size: 1432 1004
BglI GCCn_nnn'nGGC
Cuts at: 0 729 1465 2436
Size: 729 736 971
Fragments arranged by size:
971 736 729
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1214 1594 2436
Size: 1214 380 842
Fragments arranged by size:
1214 842 380
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1184 2436
Size: 385 799 1252
Fragments arranged by size:
1252 799 385
Bpu10I CC'TnA_GC
Cuts at: 0 650 2436
Size: 650 1786
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1940 2436
Size: 1940 496
BsaI GGTCTCn'nnnn_
Cuts at: 0 41 2089 2436
Size: 41 2048 347
Fragments arranged by size:
2048 347 41
BsaAI yAC'GTr
Cuts at: 0 1318 2436
Size: 1318 1118
BsaHI Gr'CG_yC
Cuts at: 0 1106 2436
Size: 1106 1330
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1913 1943 2436
Size: 1913 30 493
Fragments arranged by size:
1913 493 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 449 592 2326 2436
Size: 344 105 143 1734 110
Fragments arranged by size:
1734 344 143 110 105
BsiHKAI G_wGCw'C
Cuts at: 0 234 480 1970 2127 2384 2436
Size: 234 246 1490 157 257 52
Fragments arranged by size:
1490 257 246 234 157 52
BsmI GAATG_Cn'
Cuts at: 0 464 1432 2424 2436
Size: 464 968 992 12
Fragments arranged by size:
992 968 464 12
Bsp1286I G_dGCh'C
Cuts at: 0 234 480 1970 2127 2172 2384 2436
Size: 234 246 1490 157 45 212 52
Fragments arranged by size:
1490 246 234 212 157 52 45
BsrDI GCAATG_nn'
Cuts at: 0 1244 1982 2436
Size: 1244 738 454
Fragments arranged by size:
1244 738 454
BsrGI T'GTAC_A
Cuts at: 0 1920 2436
Size: 1920 516
BssSI C'ACGA_G
Cuts at: 0 272 2436
Size: 272 2164
BstBI TT'CG_AA
Cuts at: 0 285 423 2436
Size: 285 138 2013
Fragments arranged by size:
2013 285 138
BtgI C'CryG_G
Cuts at: 0 1764 2436
Size: 1764 672
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1980 2436
Size: 1980 456
DraI TTT'AAA
Cuts at: 0 29 710 2436
Size: 29 681 1726
Fragments arranged by size:
1726 681 29
DraIII CAC_nnn'GTG
Cuts at: 0 615 2436
Size: 615 1821
EaeI y'GGCC_r
Cuts at: 0 1562 2139 2436
Size: 1562 577 297
Fragments arranged by size:
1562 577 297
EarI CTCTTCn'nnn_
Cuts at: 0 134 613 1361 1975 2177 2436
Size: 134 479 748 614 202 259
Fragments arranged by size:
748 614 479 259 202 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 481 2436
Size: 481 1955
EcoICRI GAG'CTC
Cuts at: 0 232 2382 2436
Size: 232 2150 54
Fragments arranged by size:
2150 232 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 707 1184 1319 1360 2198 2436
Size: 385 322 477 135 41 838 238
Fragments arranged by size:
838 477 385 322 238 135 41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 1390 2436
Size: 651 739 1046
Fragments arranged by size:
1046 739 651
FspI TGC'GCA
Cuts at: 0 1622 2436
Size: 1622 814
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 366 398 2436
Size: 304 32 30 32 2038
Fragments arranged by size:
2038 304 32 32 30
HindIII A'AGCT_T
Cuts at: 0 1301 2436
Size: 1301 1135
MfeI C'AATT_G
Cuts at: 0 266 1544 2436
Size: 266 1278 892
Fragments arranged by size:
1278 892 266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1619 2091 2180 2436
Size: 364 1255 472 89 256
Fragments arranged by size:
1255 472 364 256 89
MscI TGG'CCA
Cuts at: 0 1564 2436
Size: 1564 872
MspA1I CmG'CkG
Cuts at: 0 349 1820 2436
Size: 349 1471 616
Fragments arranged by size:
1471 616 349
NdeI CA'TA_TG
Cuts at: 0 1551 2436
Size: 1551 885
NsiI A_TGCA'T
Cuts at: 0 1434 2436
Size: 1434 1002
NspI r_CATG'y
Cuts at: 0 484 1282 1527 1697 2254 2285 2436
Size: 484 798 245 170 557 31 151
Fragments arranged by size:
798 557 484 245 170 151 31
PciI A'CATG_T
Cuts at: 0 1693 2436
Size: 1693 743
PfoI T'CCnGG_A
Cuts at: 0 1096 2436
Size: 1096 1340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1510 1542 2436
Size: 1510 32 894
Fragments arranged by size:
1510 894 32
PsiI TTA'TAA
Cuts at: 0 814 2436
Size: 814 1622
PstI C_TGCA'G
Cuts at: 0 1856 1981 2436
Size: 1856 125 455
Fragments arranged by size:
1856 455 125
PvuII CAG'CTG
Cuts at: 0 349 2436
Size: 349 2087
SacI G_AGCT'C
Cuts at: 0 234 2384 2436
Size: 234 2150 52
Fragments arranged by size:
2150 234 52
SapI GCTCTTCn'nnn_
Cuts at: 0 613 1975 2177 2436
Size: 613 1362 202 259
Fragments arranged by size:
1362 613 259 202
ScaI AGT'ACT
Cuts at: 0 1205 2436
Size: 1205 1231
SfcI C'TryA_G
Cuts at: 0 1322 1852 1977 2436
Size: 1322 530 125 459
Fragments arranged by size:
1322 530 459 125
SmlI C'TyrA_G
Cuts at: 0 1273 1955 2436
Size: 1273 682 481
Fragments arranged by size:
1273 682 481
SnaBI TAC'GTA
Cuts at: 0 1318 2436
Size: 1318 1118
SphI G_CATG'C
Cuts at: 0 1282 2254 2436
Size: 1282 972 182
Fragments arranged by size:
1282 972 182
SspI AAT'ATT
Cuts at: 0 1055 1597 2436
Size: 1055 542 839
Fragments arranged by size:
1055 839 542
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 880 2436
Size: 676 204 1556
Fragments arranged by size:
1556 676 204
TatI w'GTAC_w
Cuts at: 0 1203 1920 2436
Size: 1203 717 516
Fragments arranged by size:
1203 717 516
Enzymes that do cut:
AcuI AflII AflIII AlfI AloI AlwNI ApoI BanI
BanII BbsI BbvCI BfrBI BglI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BsgI BsiHKAI BsmI
Bsp1286I BsrDI BsrGI BssSI BstBI BtgI BtgZI DraI
DraIII EaeI EarI EciI EcoICRI Eco57MI FalI FspI
Hin4I HindIII MfeI MmeI MscI MspA1I NdeI NsiI
NspI PciI PfoI PpiI PsiI PstI PvuII SacI
SapI ScaI SfcI SmlI SnaBI SphI SspI TaqII
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BsaBI BsaWI BseRI BseYI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BssHII BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DrdI EagI EcoNI EcoO109I EcoRI EcoRV
FseI FspAI HaeII HincII HpaI KasI KpnI MluI
MslI NaeI NarI NcoI NgoMIV NheI NotI NruI
PacI PasI PflMI PmeI PmlI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI
StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI