Restriction map for
pFN21AE6755
(Linear) (Six-base) MAPSORT of: KIEE6755.seq Check: 1838 from: 1 to: 1659
KIEE6755.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 1439 1659
Size: 1439 220
AclI AA'CG_TT
Cuts at: 0 1333 1659
Size: 1333 326
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1139 1262 1659
Size: 556 583 123 397
Fragments arranged by size:
583 556 397 123
AflIII A'CryG_T
Cuts at: 0 545 648 1659
Size: 545 103 1011
Fragments arranged by size:
1011 545 103
AleI CACnn'nnGTG
Cuts at: 0 607 1659
Size: 607 1052
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 1659
Size: 71 32 1556
Fragments arranged by size:
1556 71 32
ApaI G_GGCC'C
Cuts at: 0 674 1327 1659
Size: 674 653 332
Fragments arranged by size:
674 653 332
ApoI r'AATT_y
Cuts at: 0 858 883 962 1103 1659
Size: 858 25 79 141 556
Fragments arranged by size:
858 556 141 79 25
BamHI G'GATC_C
Cuts at: 0 1446 1659
Size: 1446 213
BanI G'GyrC_C
Cuts at: 0 69 1659
Size: 69 1590
BanII G_rGCy'C
Cuts at: 0 9 674 1256 1327 1535 1659
Size: 9 665 582 71 208 124
Fragments arranged by size:
665 582 208 124 71 9
BbeI G_GCGC'C
Cuts at: 0 73 1659
Size: 73 1586
BbsI GAAGACnn'nnnn_
Cuts at: 0 175 503 1540 1659
Size: 175 328 1037 119
Fragments arranged by size:
1037 328 175 119
BbvCI CC'TCA_GC
Cuts at: 0 1025 1162 1355 1659
Size: 1025 137 193 304
Fragments arranged by size:
1025 304 193 137
BciVI GTATCCnnnnn_n'
Cuts at: 0 1017 1659
Size: 1017 642
BlpI GC'TnA_GC
Cuts at: 0 686 1659
Size: 686 973
Bme1580I G_kGCm'C
Cuts at: 0 674 1327 1659
Size: 674 653 332
Fragments arranged by size:
674 653 332
BmrI ACTGGGnnnn_n'
Cuts at: 0 579 997 1452 1659
Size: 579 418 455 207
Fragments arranged by size:
579 455 418 207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1067 1659
Size: 1067 592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 643 1562 1659
Size: 455 188 919 97
Fragments arranged by size:
919 455 188 97
Bpu10I CC'TnA_GC
Cuts at: 0 1025 1162 1355 1659
Size: 1025 137 193 304
Fragments arranged by size:
1025 304 193 137
BsaAI yAC'GTr
Cuts at: 0 651 1659
Size: 651 1008
BsaHI Gr'CG_yC
Cuts at: 0 70 1659
Size: 70 1589
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 182 212 1258 1288 1659
Size: 182 30 1046 30 371
Fragments arranged by size:
1046 371 182 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1078 1282 1417 1555 1582 1659
Size: 1078 204 135 138 27 77
Fragments arranged by size:
1078 204 138 135 77 27
BseYI C'CCAG_C
Cuts at: 0 852 1030 1148 1659
Size: 852 178 118 511
Fragments arranged by size:
852 511 178 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1659
Size: 232 1427
BsiEI CG_ry'CG
Cuts at: 0 15 31 1659
Size: 15 16 1628
Fragments arranged by size:
1628 16 15
BsiHKAI G_wGCw'C
Cuts at: 0 614 687 1256 1391 1659
Size: 614 73 569 135 268
Fragments arranged by size:
614 569 268 135 73
BsmI GAATG_Cn'
Cuts at: 0 262 1659
Size: 262 1397
Bsp1286I G_dGCh'C
Cuts at: 0 9 614 674 687 1256 1327 1391 1535
Size: 9 605 60 13 569 71 64 144
Cuts at: 1535 1659
Size: 124
Fragments arranged by size:
605 569 144 124 71 64 60 13
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 126 1659
Size: 126 1533
BsrFI r'CCGG_y
Cuts at: 0 1572 1659
Size: 1572 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 479 1659
Size: 479 1180
BstYI r'GATC_y
Cuts at: 0 1446 1659
Size: 1446 213
Bsu36I CC'TnA_GG
Cuts at: 0 1042 1052 1659
Size: 1042 10 607
Fragments arranged by size:
1042 607 10
BtsI GCAGTG_nn'
Cuts at: 0 709 1005 1659
Size: 709 296 654
Fragments arranged by size:
709 654 296
EaeI y'GGCC_r
Cuts at: 0 12 28 1659
Size: 12 16 1631
Fragments arranged by size:
1631 16 12
EagI C'GGCC_G
Cuts at: 0 12 28 1659
Size: 12 16 1631
Fragments arranged by size:
1631 16 12
EarI CTCTTCn'nnn_
Cuts at: 0 478 1055 1238 1412 1659
Size: 478 577 183 174 247
Fragments arranged by size:
577 478 247 183 174
EcoICRI GAG'CTC
Cuts at: 0 1254 1659
Size: 1254 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 556 643 1139 1262 1562 1659
Size: 455 101 87 496 123 300 97
Fragments arranged by size:
496 455 300 123 101 97 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1381 1659
Size: 1381 278
EcoO109I rG'GnC_Cy
Cuts at: 0 670 671 1046 1237 1659
Size: 670 1 375 191 422
Fragments arranged by size:
670 422 375 191 1
HaeII r_GCGC'y
Cuts at: 0 73 1659
Size: 73 1586
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 715 747 1258 1290 1659
Size: 430 32 253 32 511 32 369
Fragments arranged by size:
511 430 369 253 32 32 32
HincII GTy'rAC
Cuts at: 0 1440 1659
Size: 1440 219
KasI G'GCGC_C
Cuts at: 0 69 1659
Size: 69 1590
MslI CAynn'nnrTG
Cuts at: 0 145 607 1659
Size: 145 462 1052
Fragments arranged by size:
1052 462 145
MspA1I CmG'CkG
Cuts at: 0 33 39 48 1034 1659
Size: 33 6 9 986 625
Fragments arranged by size:
986 625 33 9 6
NaeI GCC'GGC
Cuts at: 0 1574 1659
Size: 1574 85
NarI GG'CG_CC
Cuts at: 0 70 1659
Size: 70 1589
NgoMIV G'CCGG_C
Cuts at: 0 1572 1659
Size: 1572 87
NotI GC'GGCC_GC
Cuts at: 0 28 1659
Size: 28 1631
PasI CC'CwG_GG
Cuts at: 0 667 1659
Size: 667 992
PflMI CCAn_nnn'nTGG
Cuts at: 0 606 1659
Size: 606 1053
PmlI CAC'GTG
Cuts at: 0 651 1659
Size: 651 1008
PshAI GACnn'nnGTC
Cuts at: 0 1435 1659
Size: 1435 224
PspOMI G'GGCC_C
Cuts at: 0 670 1323 1659
Size: 670 653 336
Fragments arranged by size:
670 653 336
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 622 654 1659
Size: 622 32 1005
Fragments arranged by size:
1005 622 32
SacI G_AGCT'C
Cuts at: 0 1256 1659
Size: 1256 403
SalI G'TCGA_C
Cuts at: 0 1438 1659
Size: 1438 221
SapI GCTCTTCn'nnn_
Cuts at: 0 1238 1412 1659
Size: 1238 174 247
Fragments arranged by size:
1238 247 174
ScaI AGT'ACT
Cuts at: 0 1342 1659
Size: 1342 317
SfcI C'TryA_G
Cuts at: 0 434 1659
Size: 434 1225
SfoI GGC'GCC
Cuts at: 0 71 1659
Size: 71 1588
StyI C'CwwG_G
Cuts at: 0 73 1659
Size: 73 1586
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 866 1162 1659
Size: 866 296 497
Fragments arranged by size:
866 497 296
TatI w'GTAC_w
Cuts at: 0 1340 1659
Size: 1340 319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 640 672 1659
Size: 640 32 987
Fragments arranged by size:
987 640 32
XbaI T'CTAG_A
Cuts at: 0 1555 1659
Size: 1555 104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 603 1659
Size: 603 1056
XmnI GAAnn'nnTTC
Cuts at: 0 500 1659
Size: 500 1159
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AloI ApaI ApoI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrFI BstXI BstYI Bsu36I BtsI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MslI MspA1I NaeI NarI NgoMIV NotI PasI PflMI
PmlI PshAI PspOMI PsrI SacI SalI SapI ScaI
SfcI SfoI StyI TaqII TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI
AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BcgI BclI BfrBI BglI BglII BmgBI BmtI BpuEI
BsaI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI MscI NcoI NdeI NheI
NruI NsiI NspI PacI PciI PfoI PmeI PpiI
PpuMI PsiI PspXI PstI PvuI PvuII RsrII SacII
SanDI SbfI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XhoI XmaI ZraI