Restriction map for
pFN21AE6754
(Linear) (Six-base) MAPSORT of: KIEE6754.seq Check: 9710 from: 1 to: 2142
KIEE6754.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 388 511 1304 2142
Size: 388 123 793 838
Fragments arranged by size:
838 793 388 123
AccI GT'mk_AC
Cuts at: 0 317 1846 2142
Size: 317 1529 296
Fragments arranged by size:
1529 317 296
AclI AA'CG_TT
Cuts at: 0 1142 2142
Size: 1142 1000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1735 1920 2142
Size: 1735 185 222
Fragments arranged by size:
1735 222 185
AflIII A'CryG_T
Cuts at: 0 1772 2142
Size: 1772 370
AleI CACnn'nnGTG
Cuts at: 0 208 2142
Size: 208 1934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1028 2002 2142
Size: 1028 974 140
Fragments arranged by size:
1028 974 140
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 2142
Size: 118 32 1992
Fragments arranged by size:
1992 118 32
AlwNI CAG_nnn'CTG
Cuts at: 0 552 2142
Size: 552 1590
ApoI r'AATT_y
Cuts at: 0 476 1369 2142
Size: 476 893 773
Fragments arranged by size:
893 773 476
AvaI C'yCGr_G
Cuts at: 0 451 684 872 2084 2142
Size: 451 233 188 1212 58
Fragments arranged by size:
1212 451 233 188 58
BanI G'GyrC_C
Cuts at: 0 358 400 859 1242 2016 2142
Size: 358 42 459 383 774 126
Fragments arranged by size:
774 459 383 358 126 42
BanII G_rGCy'C
Cuts at: 0 29 802 993 2142
Size: 29 773 191 1149
Fragments arranged by size:
1149 773 191 29
BbeI G_GCGC'C
Cuts at: 0 863 2020 2142
Size: 863 1157 122
Fragments arranged by size:
1157 863 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 88 834 840 2142
Size: 88 746 6 1302
Fragments arranged by size:
1302 746 88 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 264 282 298 316 498 532 2142
Size: 264 18 16 18 182 34 1610
Fragments arranged by size:
1610 264 182 34 18 18 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 924 2142
Size: 924 1218
BclI T'GATC_A
Cuts at: 0 149 2142
Size: 149 1993
BglII A'GATC_T
Cuts at: 0 1553 2142
Size: 1553 589
Bme1580I G_kGCm'C
Cuts at: 0 1360 2142
Size: 1360 782
BmrI ACTGGGnnnn_n'
Cuts at: 0 1310 2142
Size: 1310 832
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1381 1764 2142
Size: 529 852 383 378
Fragments arranged by size:
852 529 383 378
Bpu10I CC'TnA_GC
Cuts at: 0 1247 1258 1670 2030 2142
Size: 1247 11 412 360 112
Fragments arranged by size:
1247 412 360 112 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 2142
Size: 1435 707
BsaI GGTCTCn'nnnn_
Cuts at: 0 1794 1964 2142
Size: 1794 170 178
Fragments arranged by size:
1794 178 170
BsaBI GATnn'nnATC
Cuts at: 0 154 2142
Size: 154 1988
BsaHI Gr'CG_yC
Cuts at: 0 281 314 860 2017 2142
Size: 281 33 546 1157 125
Fragments arranged by size:
1157 546 281 125 33
BsaWI w'CCGG_w
Cuts at: 0 1946 2142
Size: 1946 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2086 2089 2116 2119 2142
Size: 2086 3 27 3 23
Fragments arranged by size:
2086 27 23 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 321 1384 2094 2142
Size: 321 1063 710 48
Fragments arranged by size:
1063 710 321 48
BseYI C'CCAG_C
Cuts at: 0 44 62 68 86 444 504 723 2142
Size: 44 18 6 18 358 60 219 1419
Fragments arranged by size:
1419 358 219 60 44 18 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 852 1019 2142
Size: 40 812 167 1123
Fragments arranged by size:
1123 812 167 40
BsiHKAI G_wGCw'C
Cuts at: 0 29 173 215 993 1259 1295 2142
Size: 29 144 42 778 266 36 847
Fragments arranged by size:
847 778 266 144 42 36 29
BsmI GAATG_Cn'
Cuts at: 0 788 2073 2142
Size: 788 1285 69
Fragments arranged by size:
1285 788 69
Bsp1286I G_dGCh'C
Cuts at: 0 29 173 215 802 993 1259 1295 1360
Size: 29 144 42 587 191 266 36 65
Cuts at: 1360 2142
Size: 782
Fragments arranged by size:
782 587 266 191 144 65 42 36
29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 388 511 1304 1344 2142
Size: 388 123 793 40 798
Fragments arranged by size:
798 793 388 123 40
BsrDI GCAATG_nn'
Cuts at: 0 1010 2142
Size: 1010 1132
BsrFI r'CCGG_y
Cuts at: 0 415 745 1251 2142
Size: 415 330 506 891
Fragments arranged by size:
891 506 415 330
BsrGI T'GTAC_A
Cuts at: 0 833 2142
Size: 833 1309
BssSI C'ACGA_G
Cuts at: 0 822 2110 2142
Size: 822 1288 32
Fragments arranged by size:
1288 822 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2130 2142
Size: 2130 12
BstEII G'GTnAC_C
Cuts at: 0 1107 2142
Size: 1107 1035
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1383 2142
Size: 1383 759
BstYI r'GATC_y
Cuts at: 0 1553 1750 2142
Size: 1553 197 392
Fragments arranged by size:
1553 392 197
BstZ17I GTA'TAC
Cuts at: 0 1847 2142
Size: 1847 295
Bsu36I CC'TnA_GG
Cuts at: 0 178 769 1640 2142
Size: 178 591 871 502
Fragments arranged by size:
871 591 502 178
BtgI C'CryG_G
Cuts at: 0 1079 1112 2142
Size: 1079 33 1030
Fragments arranged by size:
1079 1030 33
BtsI GCAGTG_nn'
Cuts at: 0 1012 1196 1281 2142
Size: 1012 184 85 861
Fragments arranged by size:
1012 861 184 85
DrdI GACnn_nn'nnGTC
Cuts at: 0 9 2142
Size: 9 2133
EaeI y'GGCC_r
Cuts at: 0 569 743 1893 2142
Size: 569 174 1150 249
Fragments arranged by size:
1150 569 249 174
EarI CTCTTCn'nnn_
Cuts at: 0 34 178 1462 2142
Size: 34 144 1284 680
Fragments arranged by size:
1284 680 144 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 529 2142
Size: 529 1613
EcoICRI GAG'CTC
Cuts at: 0 27 991 2142
Size: 27 964 1151
Fragments arranged by size:
1151 964 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1381 1735 1764 1920 2142
Size: 529 852 354 29 156 222
Fragments arranged by size:
852 529 354 222 156 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1977 2029 2142
Size: 1977 52 113
Fragments arranged by size:
1977 113 52
EcoO109I rG'GnC_Cy
Cuts at: 0 119 879 1333 1451 1811 2142
Size: 119 760 454 118 360 331
Fragments arranged by size:
760 454 360 331 119 118
EcoRI G'AATT_C
Cuts at: 0 1369 2142
Size: 1369 773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1772 2142
Size: 1772 370
HaeII r_GCGC'y
Cuts at: 0 863 2020 2142
Size: 863 1157 122
Fragments arranged by size:
1157 863 122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 347 379 1513 1545 1944 1976 2142
Size: 347 32 1134 32 399 32 166
Fragments arranged by size:
1134 399 347 166 32 32 32
HincII GTy'rAC
Cuts at: 0 1857 2037 2142
Size: 1857 180 105
Fragments arranged by size:
1857 180 105
HindIII A'AGCT_T
Cuts at: 0 1411 2142
Size: 1411 731
HpaI GTT'AAC
Cuts at: 0 1857 2142
Size: 1857 285
KasI G'GCGC_C
Cuts at: 0 859 2016 2142
Size: 859 1157 126
Fragments arranged by size:
1157 859 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1955 2142
Size: 1955 187
MslI CAynn'nnrTG
Cuts at: 0 208 247 1828 2044 2142
Size: 208 39 1581 216 98
Fragments arranged by size:
1581 216 208 98 39
MspA1I CmG'CkG
Cuts at: 0 90 308 549 727 811 1573 2127 2142
Size: 90 218 241 178 84 762 554 15
Fragments arranged by size:
762 554 241 218 178 90 84 15
NaeI GCC'GGC
Cuts at: 0 747 2142
Size: 747 1395
NarI GG'CG_CC
Cuts at: 0 860 2017 2142
Size: 860 1157 125
Fragments arranged by size:
1157 860 125
NcoI C'CATG_G
Cuts at: 0 1112 2142
Size: 1112 1030
NdeI CA'TA_TG
Cuts at: 0 1650 2142
Size: 1650 492
NgoMIV G'CCGG_C
Cuts at: 0 745 2142
Size: 745 1397
NspI r_CATG'y
Cuts at: 0 225 1776 2142
Size: 225 1551 366
Fragments arranged by size:
1551 366 225
PasI CC'CwG_GG
Cuts at: 0 795 1784 2142
Size: 795 989 358
Fragments arranged by size:
989 795 358
PciI A'CATG_T
Cuts at: 0 1772 2142
Size: 1772 370
PflMI CCAn_nnn'nTGG
Cuts at: 0 625 2142
Size: 625 1517
PpuMI rG'GwC_Cy
Cuts at: 0 119 1333 1451 1811 2142
Size: 119 1214 118 360 331
Fragments arranged by size:
1214 360 331 119 118
PstI C_TGCA'G
Cuts at: 0 1007 1231 1981 2142
Size: 1007 224 750 161
Fragments arranged by size:
1007 750 224 161
PvuII CAG'CTG
Cuts at: 0 90 549 811 2127 2142
Size: 90 459 262 1316 15
Fragments arranged by size:
1316 459 262 90 15
SacI G_AGCT'C
Cuts at: 0 29 993 2142
Size: 29 964 1149
Fragments arranged by size:
1149 964 29
SanDI GG'GwC_CC
Cuts at: 0 1811 2142
Size: 1811 331
SapI GCTCTTCn'nnn_
Cuts at: 0 34 178 2142
Size: 34 144 1964
Fragments arranged by size:
1964 144 34
SbfI CC_TGCA'GG
Cuts at: 0 1981 2142
Size: 1981 161
SfcI C'TryA_G
Cuts at: 0 655 731 1003 1227 1977 2142
Size: 655 76 272 224 750 165
Fragments arranged by size:
750 655 272 224 165 76
SfoI GGC'GCC
Cuts at: 0 861 2018 2142
Size: 861 1157 124
Fragments arranged by size:
1157 861 124
SmaI CCC'GGG
Cuts at: 0 686 2142
Size: 686 1456
SmlI C'TyrA_G
Cuts at: 0 872 1414 2142
Size: 872 542 728
Fragments arranged by size:
872 728 542
StuI AGG'CCT
Cuts at: 0 1277 2142
Size: 1277 865
StyI C'CwwG_G
Cuts at: 0 1112 1376 1917 2142
Size: 1112 264 541 225
Fragments arranged by size:
1112 541 264 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 324 331 622 1830 1852 2074 2142
Size: 324 7 291 1208 22 222 68
Fragments arranged by size:
1208 324 291 222 68 22 7
TatI w'GTAC_w
Cuts at: 0 833 2142
Size: 833 1309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2089 2121 2142
Size: 2089 32 21
Fragments arranged by size:
2089 32 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 792 2142
Size: 792 1350
XhoI C'TCGA_G
Cuts at: 0 872 2142
Size: 872 1270
XmaI C'CCGG_G
Cuts at: 0 684 2142
Size: 684 1458
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI AlfI AloI
AlwNI ApoI AvaI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI
MmeI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PpuMI PstI PvuII SacI
SanDI SapI SbfI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BfrBI
BglI BlpI BmgBI BmtI BplI BsaAI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BssHII BstBI BtgZI ClaI
CspCI DraI DraIII EagI EcoRV FseI FspI FspAI
KpnI MfeI MluI MscI NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XmnI ZraI