Restriction map for
pFN21AE6753
(Linear) (Six-base) MAPSORT of: KIEE6753.seq Check: 4813 from: 1 to: 2007
KIEE6753.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 1472 1546 2007
Size: 5 1467 74 461
Fragments arranged by size:
1467 461 74 5
AflIII A'CryG_T
Cuts at: 0 1607 2007
Size: 1607 400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 291 2007
Size: 291 1716
AlwNI CAG_nnn'CTG
Cuts at: 0 150 1171 1539 2007
Size: 150 1021 368 468
Fragments arranged by size:
1021 468 368 150
ApoI r'AATT_y
Cuts at: 0 967 995 1185 1207 1261 1318 2007
Size: 967 28 190 22 54 57 689
Fragments arranged by size:
967 689 190 57 54 28 22
AvaI C'yCGr_G
Cuts at: 0 562 2007
Size: 562 1445
BamHI G'GATC_C
Cuts at: 0 298 532 2007
Size: 298 234 1475
Fragments arranged by size:
1475 298 234
BanI G'GyrC_C
Cuts at: 0 1141 1445 2007
Size: 1141 304 562
Fragments arranged by size:
1141 562 304
BanII G_rGCy'C
Cuts at: 0 1357 1768 2007
Size: 1357 411 239
Fragments arranged by size:
1357 411 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 655 2007
Size: 655 1352
BclI T'GATC_A
Cuts at: 0 596 2007
Size: 596 1411
BlpI GC'TnA_GC
Cuts at: 0 767 1529 2007
Size: 767 762 478
Fragments arranged by size:
767 762 478
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 770 2007
Size: 304 466 1237
Fragments arranged by size:
1237 466 304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 526 876 1583 1772 2007
Size: 139 387 350 707 189 235
Fragments arranged by size:
707 387 350 235 189 139
Bpu10I CC'TnA_GC
Cuts at: 0 85 1450 1762 2007
Size: 85 1365 312 245
Fragments arranged by size:
1365 312 245 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 22 829 872 2007
Size: 22 807 43 1135
Fragments arranged by size:
1135 807 43 22
BsaBI GATnn'nnATC
Cuts at: 0 1816 2007
Size: 1816 191
BsaWI w'CCGG_w
Cuts at: 0 383 2007
Size: 383 1624
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1581 1597 1611 1627 2007
Size: 1581 16 14 16 380
Fragments arranged by size:
1581 380 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 206 1696 2007
Size: 206 1490 311
Fragments arranged by size:
1490 311 206
BseYI C'CCAG_C
Cuts at: 0 791 827 1556 1885 2007
Size: 791 36 729 329 122
Fragments arranged by size:
791 729 329 122 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 598 2007
Size: 532 66 1409
Fragments arranged by size:
1409 532 66
BsiHKAI G_wGCw'C
Cuts at: 0 1768 2007
Size: 1768 239
Bsp1286I G_dGCh'C
Cuts at: 0 1357 1768 2007
Size: 1357 411 239
Fragments arranged by size:
1357 411 239
BsrDI GCAATG_nn'
Cuts at: 0 400 2007
Size: 400 1607
BsrGI T'GTAC_A
Cuts at: 0 2000 2007
Size: 2000 7
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1201 2007
Size: 1201 806
BstEII G'GTnAC_C
Cuts at: 0 1842 2007
Size: 1842 165
BstXI CCAn_nnnn'nTGG
Cuts at: 0 367 2007
Size: 367 1640
BstYI r'GATC_y
Cuts at: 0 198 298 332 532 1811 2007
Size: 198 100 34 200 1279 196
Fragments arranged by size:
1279 200 198 196 100 34
Bsu36I CC'TnA_GG
Cuts at: 0 1849 2007
Size: 1849 158
BtgI C'CryG_G
Cuts at: 0 895 1014 1919 2007
Size: 895 119 905 88
Fragments arranged by size:
905 895 119 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 733 957 2007
Size: 733 224 1050
Fragments arranged by size:
1050 733 224
BtsI GCAGTG_nn'
Cuts at: 0 78 650 1079 2007
Size: 78 572 429 928
Fragments arranged by size:
928 572 429 78
EaeI y'GGCC_r
Cuts at: 0 190 399 2007
Size: 190 209 1608
Fragments arranged by size:
1608 209 190
EarI CTCTTCn'nnn_
Cuts at: 0 624 2007
Size: 624 1383
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1312 2007
Size: 1312 695
EcoICRI GAG'CTC
Cuts at: 0 1766 2007
Size: 1766 241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5 139 526 876 1472 1546 1583 1772
Size: 5 134 387 350 596 74 37 189
Cuts at: 1772 2007
Size: 235
Fragments arranged by size:
596 387 350 235 189 134 74 37
5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 637 1945 2007
Size: 637 1308 62
Fragments arranged by size:
1308 637 62
EcoO109I rG'GnC_Cy
Cuts at: 0 211 1090 1566 1594 2007
Size: 211 879 476 28 413
Fragments arranged by size:
879 476 413 211 28
HaeII r_GCGC'y
Cuts at: 0 855 1707 2007
Size: 855 852 300
Fragments arranged by size:
855 852 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 236 268 581 613 933 965 1332 1364
Size: 236 32 313 32 320 32 367 32
Cuts at: 1364 1597 1629 2007
Size: 233 32 378
Fragments arranged by size:
378 367 320 313 236 233 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 348 2007
Size: 348 1659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 984 2007
Size: 279 705 1023
Fragments arranged by size:
1023 705 279
MscI TGG'CCA
Cuts at: 0 401 2007
Size: 401 1606
MslI CAynn'nnrTG
Cuts at: 0 362 976 2007
Size: 362 614 1031
Fragments arranged by size:
1031 614 362
MspA1I CmG'CkG
Cuts at: 0 271 897 1248 1399 2007
Size: 271 626 351 151 608
Fragments arranged by size:
626 608 351 271 151
NcoI C'CATG_G
Cuts at: 0 1014 1919 2007
Size: 1014 905 88
Fragments arranged by size:
1014 905 88
NspI r_CATG'y
Cuts at: 0 1611 1726 2007
Size: 1611 115 281
Fragments arranged by size:
1611 281 115
PasI CC'CwG_GG
Cuts at: 0 821 1357 2007
Size: 821 536 650
Fragments arranged by size:
821 650 536
PciI A'CATG_T
Cuts at: 0 1607 2007
Size: 1607 400
PflMI CCAn_nnn'nTGG
Cuts at: 0 408 2007
Size: 408 1599
PfoI T'CCnGG_A
Cuts at: 0 155 227 1253 2007
Size: 155 72 1026 754
Fragments arranged by size:
1026 754 155 72
PpuMI rG'GwC_Cy
Cuts at: 0 211 1090 1594 2007
Size: 211 879 504 413
Fragments arranged by size:
879 504 413 211
PspXI vC'TCGA_Gb
Cuts at: 0 562 2007
Size: 562 1445
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 304 336 2007
Size: 304 32 1671
Fragments arranged by size:
1671 304 32
PstI C_TGCA'G
Cuts at: 0 126 1086 2007
Size: 126 960 921
Fragments arranged by size:
960 921 126
PvuII CAG'CTG
Cuts at: 0 271 2007
Size: 271 1736
SacI G_AGCT'C
Cuts at: 0 1768 2007
Size: 1768 239
SacII CC_GC'GG
Cuts at: 0 898 2007
Size: 898 1109
SexAI A'CCwGG_T
Cuts at: 0 632 2007
Size: 632 1375
SfcI C'TryA_G
Cuts at: 0 122 1082 2007
Size: 122 960 925
Fragments arranged by size:
960 925 122
SmlI C'TyrA_G
Cuts at: 0 562 2007
Size: 562 1445
StuI AGG'CCT
Cuts at: 0 514 2007
Size: 514 1493
StyI C'CwwG_G
Cuts at: 0 659 1014 1919 2007
Size: 659 355 905 88
Fragments arranged by size:
905 659 355 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 396 853 1570 2007
Size: 396 457 717 437
Fragments arranged by size:
717 457 437 396
TatI w'GTAC_w
Cuts at: 0 320 2000 2007
Size: 320 1680 7
Fragments arranged by size:
1680 320 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1205 1235 1237 1267 2007
Size: 1205 30 2 30 740
Fragments arranged by size:
1205 740 30 30 2
Tth111I GACn'n_nGTC
Cuts at: 0 1864 2007
Size: 1864 143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1354 2007
Size: 1354 653
XhoI C'TCGA_G
Cuts at: 0 562 2007
Size: 562 1445
XmnI GAAnn'nnTTC
Cuts at: 0 1485 2007
Size: 1485 522
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApoI AvaI BamHI BanI
BanII BbsI BclI BlpI BmrI BpmI Bpu10I BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BsrDI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII MmeI MscI MslI MspA1I
NcoI NspI PasI PciI PflMI PfoI PpuMI PspXI
PsrI PstI PvuII SacI SacII SexAI SfcI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII Bme1580I BmgBI BmtI BplI BpuEI BsaAI BsaHI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspOMI PvuI RsrII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XmaI ZraI