Restriction map for
pFN21AE6752
(Linear) (Six-base) MAPSORT of: KIEE6752.seq Check: 849 from: 1 to: 1875
KIEE6752.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 406 1263 1875
Size: 406 857 612
Fragments arranged by size:
857 612 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 508 1366 1875
Size: 301 207 858 509
Fragments arranged by size:
858 509 301 207
AflIII A'CryG_T
Cuts at: 0 24 986 1109 1511 1875
Size: 24 962 123 402 364
Fragments arranged by size:
962 402 364 123 24
AleI CACnn'nnGTG
Cuts at: 0 1252 1875
Size: 1252 623
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 185 1875
Size: 185 1690
AlwNI CAG_nnn'CTG
Cuts at: 0 136 1011 1305 1641 1875
Size: 136 875 294 336 234
Fragments arranged by size:
875 336 294 234 136
ApaI G_GGCC'C
Cuts at: 0 752 1875
Size: 752 1123
AvaI C'yCGr_G
Cuts at: 0 751 1849 1875
Size: 751 1098 26
Fragments arranged by size:
1098 751 26
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1202 1235 1875
Size: 1202 33 640
Fragments arranged by size:
1202 640 33
BanI G'GyrC_C
Cuts at: 0 322 1875
Size: 322 1553
BanII G_rGCy'C
Cuts at: 0 752 906 1133 1875
Size: 752 154 227 742
Fragments arranged by size:
752 742 227 154
BbsI GAAGACnn'nnnn_
Cuts at: 0 591 665 1212 1291 1875
Size: 591 74 547 79 584
Fragments arranged by size:
591 584 547 79 74
BclI T'GATC_A
Cuts at: 0 1098 1875
Size: 1098 777
BfrBI ATG'CAT
Cuts at: 0 1601 1875
Size: 1601 274
BglII A'GATC_T
Cuts at: 0 176 1875
Size: 176 1699
BlpI GC'TnA_GC
Cuts at: 0 1127 1438 1726 1875
Size: 1127 311 288 149
Fragments arranged by size:
1127 311 288 149
Bme1580I G_kGCm'C
Cuts at: 0 325 752 1875
Size: 325 427 1123
Fragments arranged by size:
1123 427 325
BmrI ACTGGGnnnn_n'
Cuts at: 0 1143 1875
Size: 1143 732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 991 1875
Size: 684 307 884
Fragments arranged by size:
884 684 307
Bpu10I CC'TnA_GC
Cuts at: 0 1760 1875
Size: 1760 115
BsaI GGTCTCn'nnnn_
Cuts at: 0 28 763 1875
Size: 28 735 1112
Fragments arranged by size:
1112 735 28
BsaAI yAC'GTr
Cuts at: 0 987 1875
Size: 987 888
BsaBI GATnn'nnATC
Cuts at: 0 1714 1875
Size: 1714 161
BsaWI w'CCGG_w
Cuts at: 0 1739 1875
Size: 1739 136
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 759 1603 1875
Size: 202 557 844 272
Fragments arranged by size:
844 557 272 202
BseYI C'CCAG_C
Cuts at: 0 259 1634 1816 1875
Size: 259 1375 182 59
Fragments arranged by size:
1375 259 182 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1875
Size: 1276 599
BsiHKAI G_wGCw'C
Cuts at: 0 734 906 1133 1479 1747 1812 1875
Size: 734 172 227 346 268 65 63
Fragments arranged by size:
734 346 268 227 172 65 63
BsmI GAATG_Cn'
Cuts at: 0 340 1875
Size: 340 1535
Bsp1286I G_dGCh'C
Cuts at: 0 325 734 752 906 1133 1479 1747 1812
Size: 325 409 18 154 227 346 268 65
Cuts at: 1812 1875
Size: 63
Fragments arranged by size:
409 346 325 268 227 154 65 63
18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 406 1263 1875
Size: 406 857 612
Fragments arranged by size:
857 612 406
BsrBI CCG'CTC
Cuts at: 0 693 1875
Size: 693 1182
BsrDI GCAATG_nn'
Cuts at: 0 169 410 851 1019 1875
Size: 169 241 441 168 856
Fragments arranged by size:
856 441 241 169 168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 705 1875
Size: 705 1170
BstEII G'GTnAC_C
Cuts at: 0 912 1875
Size: 912 963
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1057 1875
Size: 1057 818
BstYI r'GATC_y
Cuts at: 0 117 176 1496 1853 1875
Size: 117 59 1320 357 22
Fragments arranged by size:
1320 357 117 59 22
BtgI C'CryG_G
Cuts at: 0 245 1080 1319 1875
Size: 245 835 239 556
Fragments arranged by size:
835 556 245 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1701 1875
Size: 1701 174
BtsI GCAGTG_nn'
Cuts at: 0 1384 1835 1875
Size: 1384 451 40
Fragments arranged by size:
1384 451 40
ClaI AT'CG_AT
Cuts at: 0 1208 1875
Size: 1208 667
EaeI y'GGCC_r
Cuts at: 0 330 638 1381 1559 1875
Size: 330 308 743 178 316
Fragments arranged by size:
743 330 316 308 178
EarI CTCTTCn'nnn_
Cuts at: 0 505 946 1549 1875
Size: 505 441 603 326
Fragments arranged by size:
603 505 441 326
EcoICRI GAG'CTC
Cuts at: 0 904 1131 1875
Size: 904 227 744
Fragments arranged by size:
904 744 227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 508 684 991 1366 1875
Size: 301 207 176 307 375 509
Fragments arranged by size:
509 375 307 301 207 176
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1249 1875
Size: 1249 626
EcoO109I rG'GnC_Cy
Cuts at: 0 1144 1302 1875
Size: 1144 158 573
Fragments arranged by size:
1144 573 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1371 1875
Size: 1371 504
HaeII r_GCGC'y
Cuts at: 0 1558 1875
Size: 1558 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 363 395 506 538 1875
Size: 102 32 229 32 111 32 1337
Fragments arranged by size:
1337 229 111 102 32 32 32
HindIII A'AGCT_T
Cuts at: 0 722 1875
Size: 722 1153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 1496 1875
Size: 702 794 379
Fragments arranged by size:
794 702 379
MscI TGG'CCA
Cuts at: 0 640 1383 1561 1875
Size: 640 743 178 314
Fragments arranged by size:
743 640 314 178
MslI CAynn'nnrTG
Cuts at: 0 352 535 946 1252 1875
Size: 352 183 411 306 623
Fragments arranged by size:
623 411 352 306 183
MspA1I CmG'CkG
Cuts at: 0 79 369 595 764 1572 1875
Size: 79 290 226 169 808 303
Fragments arranged by size:
808 303 290 226 169 79
NcoI C'CATG_G
Cuts at: 0 245 1875
Size: 245 1630
NsiI A_TGCA'T
Cuts at: 0 1603 1875
Size: 1603 272
NspI r_CATG'y
Cuts at: 0 28 1113 1515 1875
Size: 28 1085 402 360
Fragments arranged by size:
1085 402 360 28
PasI CC'CwG_GG
Cuts at: 0 1011 1875
Size: 1011 864
PciI A'CATG_T
Cuts at: 0 24 1109 1511 1875
Size: 24 1085 402 364
Fragments arranged by size:
1085 402 364 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 1305 1515 1875
Size: 1305 210 360
Fragments arranged by size:
1305 360 210
PfoI T'CCnGG_A
Cuts at: 0 182 772 875 1875
Size: 182 590 103 1000
Fragments arranged by size:
1000 590 182 103
PmlI CAC'GTG
Cuts at: 0 987 1875
Size: 987 888
PpuMI rG'GwC_Cy
Cuts at: 0 1144 1875
Size: 1144 731
PspOMI G'GGCC_C
Cuts at: 0 748 1875
Size: 748 1127
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1694 1726 1875
Size: 1694 32 149
Fragments arranged by size:
1694 149 32
PstI C_TGCA'G
Cuts at: 0 1571 1779 1875
Size: 1571 208 96
Fragments arranged by size:
1571 208 96
PvuII CAG'CTG
Cuts at: 0 79 369 595 1875
Size: 79 290 226 1280
Fragments arranged by size:
1280 290 226 79
SacI G_AGCT'C
Cuts at: 0 906 1133 1875
Size: 906 227 742
Fragments arranged by size:
906 742 227
SanDI GG'GwC_CC
Cuts at: 0 1144 1875
Size: 1144 731
SapI GCTCTTCn'nnn_
Cuts at: 0 1549 1875
Size: 1549 326
SexAI A'CCwGG_T
Cuts at: 0 992 1875
Size: 992 883
SfcI C'TryA_G
Cuts at: 0 222 918 1262 1567 1775 1875
Size: 222 696 344 305 208 100
Fragments arranged by size:
696 344 305 222 208 100
SmlI C'TyrA_G
Cuts at: 0 1849 1875
Size: 1849 26
StyI C'CwwG_G
Cuts at: 0 245 1589 1875
Size: 245 1344 286
Fragments arranged by size:
1344 286 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1440 1875
Size: 1440 435
TatI w'GTAC_w
Cuts at: 0 1468 1875
Size: 1468 407
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 768 800 1875
Size: 768 32 1075
Fragments arranged by size:
1075 768 32
XbaI T'CTAG_A
Cuts at: 0 120 1875
Size: 120 1755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 804 1875
Size: 252 552 1071
Fragments arranged by size:
1071 552 252
XhoI C'TCGA_G
Cuts at: 0 1849 1875
Size: 1849 26
XmnI GAAnn'nnTTC
Cuts at: 0 233 1875
Size: 233 1642
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI AvaI
BaeI BanI BanII BbsI BclI BfrBI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI
PspOMI PsrI PstI PvuII SacI SanDI SapI SexAI
SfcI SmlI StyI TaqII TatI TstI XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BcgI BciVI BglI BmgBI BmtI BplI
BpuEI BsaHI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PmeI PpiI PshAI PsiI PspXI PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XmaI ZraI