Restriction map for
pFN21AE6748
(Linear) (Six-base) MAPSORT of: KIEE6748.seq Check: 4872 from: 1 to: 1641
KIEE6748.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AatII G_ACGT'C
Cuts at: 0 968 1641
Size: 968 673
AccI GT'mk_AC
Cuts at: 0 1178 1641
Size: 1178 463
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1641
Size: 1177 464
AleI CACnn'nnGTG
Cuts at: 0 658 1641
Size: 658 983
AlwNI CAG_nnn'CTG
Cuts at: 0 453 1641
Size: 453 1188
ApoI r'AATT_y
Cuts at: 0 1119 1192 1641
Size: 1119 73 449
Fragments arranged by size:
1119 449 73
BanI G'GyrC_C
Cuts at: 0 1393 1641
Size: 1393 248
BanII G_rGCy'C
Cuts at: 0 643 1632 1641
Size: 643 989 9
Fragments arranged by size:
989 643 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 404 583 847 909 1641
Size: 404 179 264 62 732
Fragments arranged by size:
732 404 264 179 62
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 142 176 1641
Size: 142 34 1465
Fragments arranged by size:
1465 142 34
BclI T'GATC_A
Cuts at: 0 374 1641
Size: 374 1267
Bme1580I G_kGCm'C
Cuts at: 0 556 1085 1117 1385 1641
Size: 556 529 32 268 256
Fragments arranged by size:
556 529 268 256 32
BmgBI CAC'GTC
Cuts at: 0 927 1641
Size: 927 714
BmrI ACTGGGnnnn_n'
Cuts at: 0 1575 1641
Size: 1575 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 484 1026 1641
Size: 58 426 542 615
Fragments arranged by size:
615 542 426 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 766 1641
Size: 757 9 875
Fragments arranged by size:
875 757 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 412 1641
Size: 412 1229
BsaAI yAC'GTr
Cuts at: 0 489 1092 1641
Size: 489 603 549
Fragments arranged by size:
603 549 489
BsaBI GATnn'nnATC
Cuts at: 0 379 1641
Size: 379 1262
BsaHI Gr'CG_yC
Cuts at: 0 905 965 1641
Size: 905 60 676
Fragments arranged by size:
905 676 60
BsaWI w'CCGG_w
Cuts at: 0 294 794 1641
Size: 294 500 847
Fragments arranged by size:
847 500 294
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 850 1622 1641
Size: 850 772 19
Fragments arranged by size:
850 772 19
BseYI C'CCAG_C
Cuts at: 0 806 1641
Size: 806 835
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1641
Size: 904 737
BsmI GAATG_Cn'
Cuts at: 0 1107 1641
Size: 1107 534
BsmBI CGTCTCn'nnnn_
Cuts at: 0 182 1641
Size: 182 1459
Bsp1286I G_dGCh'C
Cuts at: 0 556 643 1085 1117 1385 1632 1641
Size: 556 87 442 32 268 247 9
Fragments arranged by size:
556 442 268 247 87 32 9
BspEI T'CCGG_A
Cuts at: 0 294 794 1641
Size: 294 500 847
Fragments arranged by size:
847 500 294
BspHI T'CATG_A
Cuts at: 0 383 1641
Size: 383 1258
BsrBI CCG'CTC
Cuts at: 0 683 1641
Size: 683 958
BsrDI GCAATG_nn'
Cuts at: 0 968 1641
Size: 968 673
BstAPI GCAn_nnn'nTGC
Cuts at: 0 882 1641
Size: 882 759
BstXI CCAn_nnnn'nTGG
Cuts at: 0 715 1641
Size: 715 926
BstYI r'GATC_y
Cuts at: 0 599 1533 1641
Size: 599 934 108
Fragments arranged by size:
934 599 108
Bsu36I CC'TnA_GG
Cuts at: 0 1066 1641
Size: 1066 575
BtgI C'CryG_G
Cuts at: 0 908 1148 1219 1539 1623 1641
Size: 908 240 71 320 84 18
Fragments arranged by size:
908 320 240 84 71 18
DrdI GACnn_nn'nnGTC
Cuts at: 0 196 1641
Size: 196 1445
EaeI y'GGCC_r
Cuts at: 0 1010 1170 1360 1641
Size: 1010 160 190 281
Fragments arranged by size:
1010 281 190 160
EarI CTCTTCn'nnn_
Cuts at: 0 631 935 1006 1641
Size: 631 304 71 635
Fragments arranged by size:
635 631 304 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 484 1026 1177 1641
Size: 58 426 542 151 464
Fragments arranged by size:
542 464 426 151 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 466 1021 1641
Size: 466 555 620
Fragments arranged by size:
620 555 466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1350 1641
Size: 1350 291
HincII GTy'rAC
Cuts at: 0 930 1641
Size: 930 711
HindIII A'AGCT_T
Cuts at: 0 323 1485 1641
Size: 323 1162 156
Fragments arranged by size:
1162 323 156
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1640 1641
Size: 743 897 1
Fragments arranged by size:
897 743 1
MscI TGG'CCA
Cuts at: 0 1012 1172 1362 1641
Size: 1012 160 190 279
Fragments arranged by size:
1012 279 190 160
MslI CAynn'nnrTG
Cuts at: 0 524 658 1641
Size: 524 134 983
Fragments arranged by size:
983 524 134
MspA1I CmG'CkG
Cuts at: 0 36 1366 1602 1641
Size: 36 1330 236 39
Fragments arranged by size:
1330 236 39 36
NcoI C'CATG_G
Cuts at: 0 908 1539 1641
Size: 908 631 102
Fragments arranged by size:
908 631 102
PmlI CAC'GTG
Cuts at: 0 1092 1641
Size: 1092 549
PstI C_TGCA'G
Cuts at: 0 86 1641
Size: 86 1555
PvuII CAG'CTG
Cuts at: 0 36 1641
Size: 36 1605
SapI GCTCTTCn'nnn_
Cuts at: 0 631 1006 1641
Size: 631 375 635
Fragments arranged by size:
635 631 375
SfcI C'TryA_G
Cuts at: 0 82 1268 1641
Size: 82 1186 373
Fragments arranged by size:
1186 373 82
SmlI C'TyrA_G
Cuts at: 0 745 772 1641
Size: 745 27 869
Fragments arranged by size:
869 745 27
SspI AAT'ATT
Cuts at: 0 11 246 1641
Size: 11 235 1395
Fragments arranged by size:
1395 235 11
StyI C'CwwG_G
Cuts at: 0 908 1397 1539 1641
Size: 908 489 142 102
Fragments arranged by size:
908 489 142 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1044 1641
Size: 1044 597
ZraI GAC'GTC
Cuts at: 0 966 1641
Size: 966 675
Enzymes that do cut:
AatII AccI AcuI AleI AlwNI ApoI BanI BanII
BbsI BcgI BclI Bme1580I BmgBI BmrI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BstAPI
BstXI BstYI Bsu36I BtgI DrdI EaeI EarI Eco57MI
EcoNI FalI HincII HindIII MmeI MscI MslI MspA1I
NcoI PmlI PstI PvuII SapI SfcI SmlI SspI
StyI TaqII ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BciVI BfrBI BglI
BglII BlpI BmtI BplI Bpu10I BsaXI BsiEI BsiHKAI
BsiWI BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI