Restriction map for
pFN21AE6747
(Linear) (Six-base) MAPSORT of: KIEE6747.seq Check: 8922 from: 1 to: 2568
KIEE6747.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 884 2529 2568
Size: 884 1645 39
Fragments arranged by size:
1645 884 39
AclI AA'CG_TT
Cuts at: 0 425 2568
Size: 425 2143
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1713 2568
Size: 260 1453 855
Fragments arranged by size:
1453 855 260
AflIII A'CryG_T
Cuts at: 0 743 938 1451 1886 2568
Size: 743 195 513 435 682
Fragments arranged by size:
743 682 513 435 195
AleI CACnn'nnGTG
Cuts at: 0 601 2568
Size: 601 1967
AlwNI CAG_nnn'CTG
Cuts at: 0 504 1011 2568
Size: 504 507 1557
Fragments arranged by size:
1557 507 504
ApoI r'AATT_y
Cuts at: 0 148 451 1120 1876 2470 2482 2506 2568
Size: 148 303 669 756 594 12 24 62
Fragments arranged by size:
756 669 594 303 148 62 24 12
AseI AT'TA_AT
Cuts at: 0 215 2194 2568
Size: 215 1979 374
Fragments arranged by size:
1979 374 215
AvaI C'yCGr_G
Cuts at: 0 310 991 1035 2568
Size: 310 681 44 1533
Fragments arranged by size:
1533 681 310 44
BamHI G'GATC_C
Cuts at: 0 1106 1551 2568
Size: 1106 445 1017
Fragments arranged by size:
1106 1017 445
BanI G'GyrC_C
Cuts at: 0 2500 2568
Size: 2500 68
BanII G_rGCy'C
Cuts at: 0 1036 2568
Size: 1036 1532
BbsI GAAGACnn'nnnn_
Cuts at: 0 641 2568
Size: 641 1927
BbvCI CC'TCA_GC
Cuts at: 0 76 977 2568
Size: 76 901 1591
Fragments arranged by size:
1591 901 76
BciVI GTATCCnnnnn_n'
Cuts at: 0 1789 2568
Size: 1789 779
BclI T'GATC_A
Cuts at: 0 2417 2568
Size: 2417 151
BfrBI ATG'CAT
Cuts at: 0 2237 2568
Size: 2237 331
BlpI GC'TnA_GC
Cuts at: 0 2275 2568
Size: 2275 293
Bme1580I G_kGCm'C
Cuts at: 0 61 1023 2568
Size: 61 962 1545
Fragments arranged by size:
1545 962 61
BmgBI CAC'GTC
Cuts at: 0 954 2568
Size: 954 1614
BmrI ACTGGGnnnn_n'
Cuts at: 0 526 2472 2568
Size: 526 1946 96
Fragments arranged by size:
1946 526 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 2568
Size: 1050 1518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1455 1586 1833 2568
Size: 1045 410 131 247 735
Fragments arranged by size:
1045 735 410 247 131
Bpu10I CC'TnA_GC
Cuts at: 0 76 977 2568
Size: 76 901 1591
Fragments arranged by size:
1591 901 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 16 107 2568
Size: 16 91 2461
Fragments arranged by size:
2461 91 16
BsaAI yAC'GTr
Cuts at: 0 744 2568
Size: 744 1824
BsaHI Gr'CG_yC
Cuts at: 0 2357 2568
Size: 2357 211
BsaWI w'CCGG_w
Cuts at: 0 14 2051 2568
Size: 14 2037 517
Fragments arranged by size:
2037 517 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2236 2266 2568
Size: 2236 30 302
Fragments arranged by size:
2236 302 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 784 1042 2568
Size: 784 258 1526
Fragments arranged by size:
1526 784 258
BseYI C'CCAG_C
Cuts at: 0 504 1861 1899 2103 2568
Size: 504 1357 38 204 465
Fragments arranged by size:
1357 504 465 204 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 963 2516 2568
Size: 814 149 1553 52
Fragments arranged by size:
1553 814 149 52
BsmI GAATG_Cn'
Cuts at: 0 1559 1865 2568
Size: 1559 306 703
Fragments arranged by size:
1559 703 306
Bsp1286I G_dGCh'C
Cuts at: 0 61 1023 1036 2568
Size: 61 962 13 1532
Fragments arranged by size:
1532 962 61 13
BspEI T'CCGG_A
Cuts at: 0 14 2568
Size: 14 2554
BspHI T'CATG_A
Cuts at: 0 1067 1328 2568
Size: 1067 261 1240
Fragments arranged by size:
1240 1067 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1019 2568
Size: 1019 1549
BsrBI CCG'CTC
Cuts at: 0 774 2568
Size: 774 1794
BsrDI GCAATG_nn'
Cuts at: 0 971 2098 2568
Size: 971 1127 470
Fragments arranged by size:
1127 971 470
BsrFI r'CCGG_y
Cuts at: 0 1349 2568
Size: 1349 1219
BsrGI T'GTAC_A
Cuts at: 0 1385 1463 2568
Size: 1385 78 1105
Fragments arranged by size:
1385 1105 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1011 2568
Size: 1011 1557
BstXI CCAn_nnnn'nTGG
Cuts at: 0 33 2568
Size: 33 2535
BstYI r'GATC_y
Cuts at: 0 782 813 999 1106 1551 2568
Size: 782 31 186 107 445 1017
Fragments arranged by size:
1017 782 445 186 107 31
Bsu36I CC'TnA_GG
Cuts at: 0 2260 2568
Size: 2260 308
BtsI GCAGTG_nn'
Cuts at: 0 735 2568
Size: 735 1833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1991 2026 2568
Size: 1991 35 542
Fragments arranged by size:
1991 542 35
DraIII CAC_nnn'GTG
Cuts at: 0 2139 2568
Size: 2139 429
EaeI y'GGCC_r
Cuts at: 0 702 1347 1457 1616 2568
Size: 702 645 110 159 952
Fragments arranged by size:
952 702 645 159 110
EarI CTCTTCn'nnn_
Cuts at: 0 49 160 170 1780 1993 2117 2165 2568
Size: 49 111 10 1610 213 124 48 403
Fragments arranged by size:
1610 403 213 124 111 49 48 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1012 2568
Size: 1012 1556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1045 1455 1586 1713 1833 2568
Size: 260 785 410 131 127 120 735
Fragments arranged by size:
785 735 410 260 131 127 120
EcoNI CCTnn'n_nnAGG
Cuts at: 0 274 644 2259 2568
Size: 274 370 1615 309
Fragments arranged by size:
1615 370 309 274
EcoO109I rG'GnC_Cy
Cuts at: 0 5 1008 1579 2568
Size: 5 1003 571 989
Fragments arranged by size:
1003 989 571 5
EcoRI G'AATT_C
Cuts at: 0 451 2568
Size: 451 2117
HaeII r_GCGC'y
Cuts at: 0 83 2275 2568
Size: 83 2192 293
Fragments arranged by size:
2192 293 83
HincII GTy'rAC
Cuts at: 0 1428 2343 2568
Size: 1428 915 225
Fragments arranged by size:
1428 915 225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1143 2267 2568
Size: 461 682 1124 301
Fragments arranged by size:
1124 682 461 301
MscI TGG'CCA
Cuts at: 0 704 1618 2568
Size: 704 914 950
Fragments arranged by size:
950 914 704
MslI CAynn'nnrTG
Cuts at: 0 388 601 1333 1684 2320 2568
Size: 388 213 732 351 636 248
Fragments arranged by size:
732 636 388 351 248 213
MspA1I CmG'CkG
Cuts at: 0 491 1548 2568
Size: 491 1057 1020
Fragments arranged by size:
1057 1020 491
NdeI CA'TA_TG
Cuts at: 0 1685 2568
Size: 1685 883
NsiI A_TGCA'T
Cuts at: 0 2239 2568
Size: 2239 329
NspI r_CATG'y
Cuts at: 0 393 933 942 1890 2568
Size: 393 540 9 948 678
Fragments arranged by size:
948 678 540 393 9
PasI CC'CwG_GG
Cuts at: 0 84 2568
Size: 84 2484
PciI A'CATG_T
Cuts at: 0 938 1886 2568
Size: 938 948 682
Fragments arranged by size:
948 938 682
PfoI T'CCnGG_A
Cuts at: 0 785 809 1563 1763 2568
Size: 785 24 754 200 805
Fragments arranged by size:
805 785 754 200 24
PmlI CAC'GTG
Cuts at: 0 744 2568
Size: 744 1824
PpuMI rG'GwC_Cy
Cuts at: 0 5 1008 2568
Size: 5 1003 1560
Fragments arranged by size:
1560 1003 5
PsiI TTA'TAA
Cuts at: 0 2071 2568
Size: 2071 497
PspXI vC'TCGA_Gb
Cuts at: 0 310 2568
Size: 310 2258
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2470 2502 2568
Size: 2470 32 66
Fragments arranged by size:
2470 66 32
PstI C_TGCA'G
Cuts at: 0 53 1007 2568
Size: 53 954 1561
Fragments arranged by size:
1561 954 53
PvuII CAG'CTG
Cuts at: 0 491 1548 2568
Size: 491 1057 1020
Fragments arranged by size:
1057 1020 491
SanDI GG'GwC_CC
Cuts at: 0 5 2568
Size: 5 2563
SapI GCTCTTCn'nnn_
Cuts at: 0 49 170 2568
Size: 49 121 2398
Fragments arranged by size:
2398 121 49
ScaI AGT'ACT
Cuts at: 0 2224 2568
Size: 2224 344
SexAI A'CCwGG_T
Cuts at: 0 1751 2135 2568
Size: 1751 384 433
Fragments arranged by size:
1751 433 384
SfcI C'TryA_G
Cuts at: 0 49 885 1003 1170 2568
Size: 49 836 118 167 1398
Fragments arranged by size:
1398 836 167 118 49
SmlI C'TyrA_G
Cuts at: 0 310 1035 2568
Size: 310 725 1533
Fragments arranged by size:
1533 725 310
SspI AAT'ATT
Cuts at: 0 191 1712 2192 2568
Size: 191 1521 480 376
Fragments arranged by size:
1521 480 376 191
StuI AGG'CCT
Cuts at: 0 98 2568
Size: 98 2470
StyI C'CwwG_G
Cuts at: 0 543 1590 2568
Size: 543 1047 978
Fragments arranged by size:
1047 978 543
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1433 1493 2568
Size: 1433 60 1075
Fragments arranged by size:
1433 1075 60
TatI w'GTAC_w
Cuts at: 0 380 825 1385 1463 2222 2568
Size: 380 445 560 78 759 346
Fragments arranged by size:
759 560 445 380 346 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2067 2099 2568
Size: 2067 32 469
Fragments arranged by size:
2067 469 32
Tth111I GACn'n_nGTC
Cuts at: 0 1519 2568
Size: 1519 1049
XbaI T'CTAG_A
Cuts at: 0 282 2568
Size: 282 2286
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2143 2568
Size: 2143 425
XhoI C'TCGA_G
Cuts at: 0 310 1035 2568
Size: 310 725 1533
Fragments arranged by size:
1533 725 310
XmnI GAAnn'nnTTC
Cuts at: 0 1738 2482 2568
Size: 1738 744 86
Fragments arranged by size:
1738 744 86
Enzymes that do cut:
AccI AclI AcuI AflIII AleI AlwNI ApoI AseI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI Bsu36I BtsI CspCI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
HincII MmeI MscI MslI MspA1I NdeI NsiI NspI
PasI PciI PfoI PmlI PpuMI PsiI PspXI PsrI
PstI PvuII SanDI SapI ScaI SexAI SfcI SmlI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI
BcgI BglI BglII BmtI BpuEI BsaBI BsiEI BsiHKAI
BsiWI BsmBI BssHII BssSI BstBI BstEII BstZ17I BtgI
BtgZI ClaI DraI DrdI EagI EcoICRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PflMI PmeI PpiI PshAI PspOMI PvuI
RsrII SacI SacII SalI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XmaI
ZraI